scholarly journals Morphological and Anatomical Changes by Cochicine in Seedling of Impatiens balsamina L.

Author(s):  
Ni Made Sastriyani Wiendra ◽  
Made Pharmawati

Impatiens balsamina L. is a plant that is widely cultivated in Bali. The flower of this plant is used as a component of offering in Hindu religious ceremonies. The flower petals are thin and wilt easily, therefore genetic modification is needed to get better quality. This research aimed to study the effect of 0.01% colchicine with soaking periods of 4 hours, 8 hours, 12 hours and 24 hours to seedlings of I. balsamina. Seeds of I. balsamina were germinated and sprouted seeds were treated with 0.01% colchicine for 4 hours, 8 hours, 12 hours and 24 hours. The sprouted seeds were then transfer to polybag with soil media. The experiment was designed with randomized blok design with five replicates. Observations were done on plant height, length and width of leaf, stem diameter, number of stomata as well as observation on plant stem cortex cells. Results showed that colchicine reduced plant height at three weeks after planting, while plant stem diameter increased. Control plants had the smallest leaf length and width. The number of stomata decreased at plants derived from colchicine treated sprouted seeds and the lowest stomatal number observed was at soaking duration of 24 hours.

2020 ◽  
Vol 8 (2) ◽  
pp. 61
Author(s):  
. HOBIR

<p>Pengaruh ukuran dan perlakuan bibit terhadap petumbuhan dan produksi umbi iles-ilcs (Amorphophallus oncophyllus) ditcliti di Bogor dan Cikampek tahun 1993 1995, yang dilakukan dalam dua percobaan. Pada percobaan petama dipelajai pengaruh ukuran bibit utuh dan pada percobaan kedua pengaruh ukuran dan pcmbclahan bibit terhadap petumbuhan dan produksi umbi. Bibit utuh berasal dari umbi batang dengan ukuran 200 dan lOOg, seta umbi tetas dengan 5 dan 2.5g, sedangkan pcmbclahan bibit berasal dai umbi batang ukuran 200g, yang dibelah menjadi 2 bagian (a lOOg), 4 bagian (a SOg) dan 8 bagian (a 25g). Bibit dai pcrlakuan-perlakuan tersebut disemai di bak pasir di rumah kaca dan petumbuhannya diamati pada umur 1-2 bulan. Bibit yang bctunas normal ditanam di lapangan. Untuk percobaan percobaan petama (bibit utuh), bibit-bibit yang bctunas normal di tanam di Bogor dan Cikampek, sedang percobaan kedua (bibit dibelah) ditanam hanya di Bogor. Kedua percobaan tersebut dirancang secara acak kelompok dengan 5 ulangan. Parameter yang digunakan untuk menilai pengaruh perlakuan adalah tinggi tanaman dan diameter batang, panjang daun dan produksi umbi pada umur 6 dan 18 bulan setelah tanam, untuk bibit utuh dan 6 bulan untuk bibit yang dibelah. Hasil penelitian dapat disimpulkan sebagai berikut. Dai percobaan pertama (bibit utuh) temyata ukuran bibit tidak berpengaruh terhadap persentase pertunasan maupun petumbuhan di lapang. Komponen pertumbuhan dan hasil umbi sangat dipengaruhi oleh lokasi penanaman dan ukuran bibit. Komponen pertumbuhan, kecuali diameter batang, seta produksi umbi di Bogor nyata lebih tinggi dari pada di Cikampek. Pada bibit utuh, ukuran bibit 200g menghasilkan komponen petumbuhan dan produksi umbi paling tinggi dan berbeda dengan ukuran- ukuran lainnya, makin kecil ukuran bibit makin kecil nilai komponen petumbuhan dan produksi umbi. Dai percobaan kedua (pembelahan umbi) diperoleh bahwa pembelahan umbi dai 200g sampai 25g tidak mempengaruhi pertunasan di rumah kaca maupun tanaman yang tumbuh di lapangan. Tinggi batang lebih kecil pada bclahan 25g, diameter batang pada belahan 50g dan produksi umbi pada bclahan lOOg dibanding bibit utuh 200g. Berdasarkan data tersebut, untuk memproduksi bibit secara masal, umbi dapat dibelah sampai ukuran 25g dan dalam waktu 6 bulan dapat diperoleh umbi sebcrat + 200g dan umbi ini dapat menghasilkan umbi sekitar 1 kg umbi/batang dalam waktu 6 bulan.</p><p>Kata kunci: Amorphophallus oncophyllus. ukuran bibit, produksi</p><p> </p><p><strong>ABSTRACT</strong></p><p><strong>Effect of seed size and seed treatment on the growth and yield of Amorphophallus oncophyllus</strong></p><p>Efect of seed size and seed treatment on the growth and yield of Amorphophallus oncophyllus was studied in Bogor and Cikampek from 1993 to 1995, in two expeiments. The irst experiment studied the efect of the size of sound seed, and the second studied the efect of size and splitting seed on die growth and yield of tuber. In the first experiment the sound seed used were the true tuber with the size of 200 and lOOg and bulbil with the size of 5 and 2.5g. In the second expeiment, the tuber of 200g were splined into 2 parts (a lOOg), 4 parts (a 50g) and 8 parts (a 25g). The seeds were irstly planted in sand boxes in die glass house and evaluated for their sprouting at I -2 month ater planting. For the irst expeiment the normal sprouted seeds were planted in the ield in Bogor and Cikampek, while for the second expeiment, the normal sprouted seeds were planted in Bogor The two experiments were designed as a randomized block in 5 replicates. The parameters used for evaluating the effect of treatments were plant height, stem (pseudo-stem) diameter, leaf length and yield of tuber at 6 and 18 months ater planting for the irst and 6 months for the second expeiment. Results of the studies are summarized as follows. From the irst expeiment (sound seed) it was sevealed that seed size neither afected spronting percentage nor plant stand in the ield. The growth components and tuber yield were afected by planting location and size of seed. The growth components, except stem diameter, and tuber yield in Bogor significantly higher than those in Cikampek. From the second experiment it was shown that splitting seed rom 100 to 25g neither afected the sprouting percentage in the glass house nor plant stand in the ield. Plant height signiicantly decreased at the size of 25g, stem diameter al 50g and tuber yield at lOOg. Based on the data, for mass production of seed tuber, the tuber could be splined into 25g. Within 6 months the seed may produced a tuber of 300g and the tuber could produced 1 kg tuber within 6 months.</p><p>Key words : Amorphophallus oncophyllus. seed size, tuber yield</p>


2014 ◽  
Vol 36 (3) ◽  
pp. 693-703 ◽  
Author(s):  
Rodinei Facco Pegoraro ◽  
Bruna Aparecida Madureira de Souza ◽  
Victor Martins Maia ◽  
Uirá do Amaral ◽  
Marlon Cristian Toledo Pereira

This study aimed to evaluate the growth characteristics of irrigated Vitória pineapple plants grown in semi-arid conditions and determine its developmental stages based on those characteristics. It was used a randomized block design with four replicates. The experimental treatments were: plant harvest at 270, 330, 390, 450, 510, 570, 690, 750, and 810 days after planting (DAP). The following variables were determined: plant height, stem diameter, D-leaf length, D-leaf fresh and dry mass, biomass production of plants and plant parts (organs), and vegetative biomass. Five phenological stages are proposed based on vegetative biomass production: < 20% biomass production (V1); 21-40% (V2); 41-60% (V3); 61-80% (V4); and > 80% (V5). The maximum growth rate for plant height, D-leaf length, and stem diameter was observed at the end of the phenological stage V1 (390-411 DAP), and at the end of stage V5 these plant traits had average values of 106, 82, and 7 cm, respectively. The maximum biomass accumulation rates were observed at stages V4 and V5, resulting in a final fruit yield and total fresh biomass of 72 t ha-1 and 326 t ha-1, respectively. Finally, we estimated that 80% of the accumulated biomass may remain in the field after fruit and slip harvest, and could be incorporated as plant residue into the soil.


2019 ◽  
Vol 17 (1) ◽  
pp. 175-187
Author(s):  
R Rai ◽  
J Shrestha ◽  
JH Kim

An experiment was carried out using eight double cross F1s (DCF1s) as lines and two testers to obtain sixteen Line ×Tester hybrids for the evaluation of major growth and flowering traits thereby to understand the breeding potentiality of the parental lines. The performance of growth and flowering traits of all twenty-six genotypes were evaluated in a randomized complete block design (RCBD) with three replications. The analysis of variance (ANOVA) showed all the genotypes were significantly different for all agro-morphological traits under study. The estimated general combining ability (GCA) effects showed that parent 5(P5), were superior for plant height, stem diameter, number of leaves, leaf length, days to flowering and attitude of floral axis followed by parent 2(P2) for leaf length, leaf width, days to flowering and attitude of floral axis and parent7 (P7) for number of flower, flower diameter, bud length and attitude for floral axis. Likewise; tester 1 found to be best combiner with significant GCA effect for almost all growth and flowering traits (except number of leaves). The plant height, leaf width, number of flower and attitude of floral axis showed prevailing additive gene action while stem diameter, number of leaves, leaf length, days to flowering, flower diameter and bud length indicated predominance of dominance gene action. We can conclude that parent P5, parent P2 and parent P7 can be used as good mother lines and the tester 1, as good donor for the seed production. SAARC J. Agri., 17(1): 175-187 (2019)


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Bagus Santoso ◽  
Amarullah Amarullah ◽  
Dwi Santoso

ABSTRACT  Chili Plants (Capsicum annum L.) originating from the tropics and subtropicsAmericas, especially Colombia, South America, and continue to spread to Latin America. Evidence of chili cultivation was first discovered in Peru's historical siteand leftover seeds that are more than 5000 years old BC in a cave in Tehuacan, Mexico. The spread of chili to the whole world including countries in Asia, like Indonesia carried out by Spanish and Portuguese traders. The purpose of this study was to determine the effect of electromagnetic radiation on the growth and yield of red chilies in the city of Tarakan.This research was conducted from September 2018 to January 2019, at the screen house of the Faculty of Agriculture, Borneo Tarakan University. This study was prepared using factorial Randomized Block Design (RBD) with 5 treatments repeated 5 times. The factors studied were large electromagnetic radiation 56 microtesla with exposure time (P0) 0 minutes, (P1) 20 minutes, (P2) 40 minutes, (P3) 60 minutes, (P4) 80 minutes. Observation parameters; plant height, number of leaves, leaf length, leaf width, stem diameter and number of branches. Analysis of variance using variance ANOVA with a confidence level of 95% and if it is significantly different it will be continued with DMRT further testing.The results of the study show that: 1) Observation parameters that showed statistically significant differences were parameters of plant height, number of branches and number of leaves while parameters that did not show different results were parameters of leaf length, leaf width and stem diameter., 2) The best treatment for each observation parameter is P2 treatment (exposure to electromagnetic radiation for 40 minutes), and the lowest treatment is treatment P0 (without exposure to electromagnetic radiation). Suggestions from this study should be carried out further research on the use of electromagnetic radiation in the vegetative phase until the results of red chili plants (Capsicum annum L.)Keywords: chili, electromagnetics radiation, Randomized Block Design  ABSTRAK Tanaman cabai (Capsicum annum L.) berasal dari dunia tropika dan subtropika Benua Amerika, khususnya  Colombia, Amerika Selatan, dan terus menyebar ke Amerika Latin. Bukti budidaya cabai pertama kali ditemukan dalam tapak galian sejarah Peru dan sisaan biji yang telah berumur lebih dari 5000 tahun SM di dalam gua di Tehuacan, Meksiko. Penyebaran cabai ke seluruh dunia termasuk negara-negara di Asia, seperti Indonesia dilakukan oleh pedagang Spanyol dan Portugis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh radiasi elektromagnetik terhadap pertumbuhan dan hasil tanaman cabai merah di kota Tarakan. Penelitian ini dilaksanakan pada tahun 2018, di screen house Fakultas Pertanian Universitas Borneo Tarakan. Penelitian ini disusun dengan menggunakan Rancangan Acak Kelompok (RAK) faktorial dengan 5 perlakuan diulang sebanyak 5 kali. Faktor yang diteliti yaitu besar radiasi elektromagnetik 56 mikrotesla dengan lama pemaparan (P0) 0 menit, (P1) 20 menit, (P2) 40 menit, (P3) 60 menit, (P4) 80 menit. Parameter pengamatan; tinggi tanaman, jumlah daun, panjang daun, lebar daun, diameter batang dan jumlah cabang. Analisis ragam menggunakan sidik ragam Anova dengan tingkat kepercayaan 95% dan apabila berbeda nyata maka akan dilanjut dengan uji lanjut DMRT. Hasil penelitian menunjukkan bahwa: 1) Parameter pengamatan yang menunjukkan hasil berbeda nyata secara statistik adalah parameter tinggi tanaman, jumlah cabang dan jumlah daun sedangkan parameter yang tidak menunjukkan hasil yang berbeda nayata adalah parameter panjang daun, lebar daun dan diameter batang, 2) Perlakuan terbaik untuk setiap parameter pengamatan adalah perlakuan P2 (pemaparan radiasi elektromagnetik selama 40 menit), dan perlakuan terendah adalah perlakuan P0 (tanpa pemaparan radiasi elektromagnetik). Saran dari penelitian ini sebaiknya dilakukan penelitian lanjutan mengenai penggunaan radiasi elektromagnetik pada fase vegetatif sampai hasil tanaman cabai merah (Capsicum annum L.)Kata kunci : cabai merah, radiasi elektromagnetik, Rancangan Acak Kelompok


Author(s):  
J. Irawan ◽  
A. B. Saidi ◽  
W. Hadianto

This research aimed to observe the appearance of Coix lacryma-jobi L. as affected by number of seeds and plant spacing. Randomized Blok Design comprised of 2 factors (number of seeds, J; plant spacing, T) with 3 replications was arranged, while the parameters included plant height, stem diameter, number of leaf, length and width of leaf. The experiment was conducted at experimental field of Agriculture Faculty in Teuku Umar University, Meulaboh, from March to October 2017. The results showed that the number of seeds significantly affected plant height in 49 days after planting, 63 days after planting, and also showed a significant effect on leaf length in 77 days after planting. Statitical analysis showed the interaction effect among the factors. The most significant effect was observed in plant height and leaf length in 21 days after planting.


2014 ◽  
Vol 11 (4) ◽  
pp. 1441-1447
Author(s):  
Baghdad Science Journal

The experiment was carried out to study the effect of NPK and Inicium using two concentration for each of them 1 and 2 gm/L for NPK and 5 and 10 ml/L for Inicium on some physiological characters of Lycopersicon esculentum and mitotic division index of seedling radical apex. The results showed that all the characters increased plant height, stem diameter, leaves number, length of leaf, lobs number in one leaf, length of root and root diameter. The data showed that the percentage of cell division, mitotic division and Telophase stage increased in seedling radical apex comparison with control plants.


2016 ◽  
Vol 4 (11) ◽  
pp. 115-121
Author(s):  
Amana Mama ◽  
Jemal Jeylan ◽  
AbebeWoldesenbet Aseffa

The experiment was conducted under field condition at Jimma University College of Agricultures and Veterinary Medicine (JUCAVM) to determine the Effects of Different Rate of Organic and Inorganic Fertilizer on Growth and yield Components of Potato (Solanum tuberosumL).The experiment design used was randomized complete block design(RCBD). The experimental treatments were replicated three times. A Potato cultivar ‘Jalene’ was grown combined with Farmyard manure and nitrogen (F0N0), (F20N70), (F0N35),(F0N70), (F20N35), (F20N0) as a treatment.. Data were recorded on Potato leaf area, plant height, leaf length, leaf number, leaf diameter, stem diameter and stem number. The result revealed that the combination of various level of Farmyard manure and nitrogen statistically (p≤0.05) affected leaf area, plant height, leaf length, leaf number, leaf diameter, stem diameter and stem number. The maximum values for leaf area, plant height, leaf length, leaf number, leaf diameter, stem diameter and stem number were recorded from (F0N70, F20N70, F0N70, F20N35, F20N0, F20N70, F20N0) respectively .However The minimum values of all the parameters were recorded from the control treatment (F0 N0). From the study it could be concluded that Potato yield components were greatly affected by different rates of organic and inorganic fertilizers. The appropriate amount of different rates of fertilizers (farmyard manure and nitrogen) should be used to increase Potato growth and yield.


Author(s):  
Cándido Enrique Guerra Medina ◽  
CARLOS HUGO AVENDAÑO ARRAZATE ◽  
Oziel Dante Montañez-Valdez ◽  
Jose de Jesus Madonado Méndez ◽  
Alejandro Ley de Coss

Objetive: To evaluate the growth, chemical composition and quality of the silage of 45 d and 60 d cuts of Pennisetum purpureum sp. Gigante and Pennisetum purpureum cv. Taiwan grasses. Design/methodology/approximation: The study took place at the Germplasm Bank of the Rosario Izapa Experimental Field in Tuxtla Chico, Chiapas, Mexico. Chemical analysis of the silage determined crude protein (CP), crude fat (CF), crude fiber (CF), ash (A), neutral detergent fiber (NDF), acid detergent fiber (ADF), and pH. Plant height, leaf length, leaf width and stem diameter were also determined. A completely randomized design was used with three repetitions per treatment as per PROC GLM and means comparison using the Tukey procedure. Results: The CP content of both grasses decreased in the 45 d to 60 d period; from 7.18 % to 5.36 % in Taiwan grass and from 7.53 % to 6.05 % in Pennisetum sp. Gigante. Plant height, length and width of leaf, and stem diameter were greater in the Taiwan variety (P<0.05) at 30 d, 45 d and 60 d; the pH of the silage was similar in both grasses evaluated (P>0.05) at 45 d and 60 d. Study limitations /implications:  A review during low water periods is required (December-April). Findings/conclusions: Taiwan grass registered a greater growth rate in all periods; while Gigante grass showed higher nutritional value at 45 and 60 days of cutting.


2020 ◽  
Vol 8 (1) ◽  
pp. 111
Author(s):  
Fransiskus Palobo ◽  
Siska Tirajoh ◽  
Merlin K. Rumbarar ◽  
Muhammad Thamrin

One effort to increase corn production is through the use of new superior varieties (NSV) hybrid corn and composite corn. This study aims to determine the performance of growth and yields of four NSV hybrid corn and composite corn. The study was conducted in Merauke Regency, Papua since August to December 2018. The design used was Randomized Group Design (RGD) based on one factorial with 5 sub factors, each hybrid corn; HJ 21 Nasa variety, Nasa 29 variety and composite corn; Lamuru variety, Bisma variety, Sukmaraga variety. The results of the study at various ages of plant height were not affected significantly, the number of leaves were significantly affected by 30 days after planting (DAP) and at harvest time. Leaves width at various ages had no significant effect, while leaves length and stem diameter were all observed at the highest leaf length with the effect of 30 days after planting (DAP) Nasa 29 (75 cm) but 60 days after planting (DAP) and at harvest HJ 21 Agritan 93 cm. Then the largest stem diameter of Nasa 29 (1.7 cm) was 30 days after planting (DAP) and 60 days after planting (DAP)- at harvest was each 2.5 cm. Difference of the longest cob length of HJ 21 agritan 38.4 cm, diameter of the largest cob 5.1 cm, the highest number of lines per cob 15.8 seeds, the heaviest weight of 1000 HJ 21 Agritan 420.6 g, heaviest dry harvest Nasa 29 (12.4 t/ha) and the heaviest dry pipelines Nasa 29 (9.2 t/ha). The overall performance of the results significantly affected both hybrid corn and composite corn.


2012 ◽  
Vol 5 (1) ◽  
pp. 309-318 ◽  
Author(s):  
MI Kabir ◽  
MG Mortuza ◽  
MO Islam

The experiment was conducted to see the effect of nutrient spray on morphophysiological feature and growth of three orchid varieties namely Dendrobium Red Bull, D. Kasim Gold and D White 5 N. Results revealed that the morphophysiological and growth attributes significantly varied among the cultivars. D Red Bull showed the highest plant height, leaf length, leaf area and stem diameter among the varieties. D. White 5 N was superior in leaf number and total leaf area and D. Kasim Gold was superior in leaf area index and leaf width to the other varieties. On the other hand, the trend of increasing in leaf length, leaf width, leaf area index, leaf number, leaf area and total leaf area was the highest for N:P:K as 10:25:30. Plant height and stem diameter was maximum for N:P:K as 15:20:20. In conclusion, low level of nitrogen and high level of phosphorus and potassium was suitable for leaf length, leaf width, leaf area index, leaf number, leaf area and total leaf area while high level of nitrogen and low level of potassium was suitable for plant height and stem diameter. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11598 J. Environ. Sci. & Natural Resources, 5(1): 309-318, 2012


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