scholarly journals ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOID DARI EKSTRAK DAUN JAMBU BIJI PUTIH (Psidium guajava Linn)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Egi Azikin Maulana ◽  
I. A. R. Astiti Asih ◽  
Made Arsa
Keyword(s):  
Antioxidant Activity ◽  
Psidium Guajava ◽  
Hexane Extract ◽  
Flavonoid Compound ◽  
Separation And Purification ◽  
Chromatography Method ◽  
Oh Groups ◽  
Butanol Extract ◽  
Dpph Method ◽  
Guava Leaf

Antioxidant is molecules that can inhibit the activity of free radical. White guava (Psidium guajava Linn) is one of many plants that have potential as antioxidant. This research was aimed to identify the flavonoid compounds and to determine IC50 values in the white guava leaf extract. Extraction was done by maceration method. The separation and purification was done by TLC and coloumn chromatography method. Flavonoid compound was identified using FTIR and UV-Vis spectrofotometry and antioxidant activity was tested with DPPH method. A 1000 grams of white guava leaf powder was extracted by n-hexane produced 103,40 grams of concentrated n-hexane extract and extraction procces by ethanol 70 % produced 128,49 grams of concetrated etanol extract. Eighty grams of ethanol extract partition produced 1.01 grams of n-hexane extract, 1.95 grams of chloroform extract, and 3.77 grams of n-butanol extract. The results of phytochemical test indicated that n-butanol extract positively contained flavonoids. The results of antioxidant activity towards DPPH from n-butanol extract showed IC50 of 37,14 ppm. The separation and purification n-butanol extract with n-hexane : ethyl acetate : n-butanol (8:2:1) as the eluent resulted 5 fractions (A, B, C, D, E), with C fraction positively contained flavonoids. FTIR analysis showed C isolates contained functional groups of CH aliphatic, CH aromatic, C = C aromatic, C = O, OH, and C - O. C fraction was analyzed with UV-Vis showing 2 peaks at ? 347.30 nm (band I) and ? 278.50 nm (band II) suggesting the presence of flavones group with possibility of OH groups attached to C-3, C-2’ atoms, ortho-dihydroxy groups attached to C-5', C- 6; C-4', C-5', and O-glycoside group at C-7 atom.

scholarly journals Kandungan Antioksidan Daun Mahang Damar (Macaranga triloba (Bl.) Muell Arg.)

2018 ◽  
Vol 3 (2) ◽  
pp. 122-131
Author(s):  
Pienyani Rosawanti ◽  
Dewi Sari Mulia ◽  
Syahrida Dian Ardhany
Keyword(s):  
Antioxidant Activity ◽  
Economic Value ◽  
Ethyl Acetate Fraction ◽  
Hexane Extract ◽  
Flavonoid Compound ◽  
Test Results ◽  
Average Value ◽  
Dpph Method ◽  
Similar Content

In the year 2015, the plants that initially grow a lot of wild is increasing in popularity because many contested by the citizens. The reason is because the plant has an economic value. Based on the literature review it is known that apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanolic extract of Macaranga gigantia leaf. Compounds that have potential as antioxidants are generally compounds of flavonoids, phenolics, and alkaloids. Flavonoid and polyphenolic compounds are antioxidant, antidiabetic, anticancer, antiseptic, and anti-inflammatory, while alkaloids have antineoplastic properties that also inhibit the growth of cancer cells. Phenol content, antioxidant activity and antibacterial activity were also found in Macaranga gigantea, M. hosei, M. hypoleuca, M. kingii, M. pruinosa and M. triloba (Lim et.al., 2014). This leads to the notion that mahang leaves were obtained and collected from the Central Kalimantan region where this study carried out also contained similar content and activity. Determination of the value of antioxidant activity in this study using DPPH method performed on n-hexane extract ofmahang leaf obtained by maceration. N-hexane extract of mahang leaf have antioxidant activity, this can be seen from the preliminary TLC test results where the spots produced when sprayed with DPPH fluoresce when viewed at 366 nm uv. The average value of IC50n-hexane extract of mahang leaves was 141.3 ppm.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

2019 ◽  
Vol 7 (1) ◽  
pp. 86-91
Author(s):  
Fredryk W Mandey ◽  
Endah Handayani ◽  
Wahyuni Eka Nanda ◽  
Alfian Noor
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Steroid Alkaloid ◽  
South Sulawesi ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

scholarly journals Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

2019 ◽  
Vol 7 (1) ◽  
pp. 86-91
Author(s):  
Fredryk W Mandey ◽  
Endah Handayani ◽  
Wahyuni Eka Nanda ◽  
Alfian Noor
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Steroid Alkaloid ◽  
South Sulawesi ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Sawo Manila (Acrhras Zapota L.) Menggunakan Metode DPPH (1,1-diphenyl-2-picrylhydrazil)

2021 ◽  
Vol 16 (1) ◽  
pp. 1
Author(s):  
Rugayyah Alyidrus ◽  
A. Suparlan Isya Syamsu ◽  
Nurjannah Nurjannah
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Wave Length ◽  
Ethanol Extract ◽  
Positive Control ◽  
Flavonoid Compound ◽  
Dpph Method

ABSTRACT Extract Ethanol of Manila Sapodilla (Acrhras zapota L.)  contain flavonoid compound with antioxidant activity. Aim of this research is to find out ethanol  extract of manila sapodilla (Acrhras zapota L.) has antioxidant activity with DPPH method (1,1-diphenyl-2-picrylhydrazil). Extract was made by using maceration  method by using ethanol liquid 70%. Ethanol extract of manila sapodilla (Acrhras zapota L.) was made main liquid 1000 ppm after that therewere dilution of 20, 40, 60, and 80 ppm. Positive control (Vitamin C) with concentration 2, 4, 6, and 8 ppm. Each of concentration was taken 1 ml then added 2 ml of DPPH (1,1-diphenyl-2-picrylhydrazil) liquid, and then it is  homogent and incubated during 30 minutes. After that, measurement of spectrophotometer UV-Vis was conducted in wave length of 517 nm. Result of data was analyzed by using absorbansi with grapic of concentration relationship with percentage of DPPH (1,1-diphenyl-2-picrylhydrazil) inhibition. Result of research shows that ethanol  extract of manila sapodilla (Acrhras zapota L.) was proven has the antioxidant activity very strong with IC50  8,2786 µg/mL. Keywords : Manila Sapodilla, DPPH, Antioxidant, Spectrophotometer. ABSTRAK Ekstrak etanol daun sawo manila (Acrhras zapota L.)  mengandung senyawa flavonoid yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini untuk mengetahui apakah ekstrak etanol daun sawo manila (Acrhras zapota L.) memiliki aktivitas antioksidan dengan metode DPPH (1,1-diphenyl-2-picrylhydrazil). Ekstrak dibuat dengan metode maserasi menggunakan pelarut etanol 70%. Ekstrak etanol daun sawo manila (Acrhras zapota L.) dibuat larutan induk 1000 ppm setelah  itu dilakukan pengenceran 20, 40, 60, dan 80 ppm. Kontrol positif (Vitamin C) dengan konsentrasi 2, 4, 6, dan 8 ppm. Dari masing-masing konsentrasi diambil 1 ml lalu di tambahkan 2 ml larutan DPPH (1,1-diphenyl-2-picrylhydrazil), kemudian dihomogenkan dan diinkubasi selama 30 menit. Setelah itu,dilakukan pengukuran spektrofotometer UV-Vis pada panjang gelombang 517 nm. Data hasil dianalisis pengukuran absorbansi menggunakan grafik hubungan konsentrasi dengan persen inhibisi DPPH (1,1-diphenyl-2-picrylhydrazil). Hasil penelitian menunjukkan bahwa ekstrak etanol daun sawo manila (Acrhras zapota L.) terbukti memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50  8,2786 µg/mL. Kata kunci : Sawo Manila, DPPH, Antioksidan, Spektrofotometer. 

scholarly journals Antioxidant Activity of Secondary Metabolite Compounds from Lichen Teloschistes flavicans

2021 ◽  
Vol 11 (6) ◽  
pp. 13878-13884
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Scientific Development ◽  
Molecular Formula ◽  
Separation And Purification ◽  
Specific Chemical ◽  
Antioxidants Activity ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values

In the last decade, lichen has become a research interest related to exploring secondary metabolites compounds. Lichen is a unique organism and contains specific chemical compounds rarely found in other natural sources; one species is the lichen Teloschistes flavicans. The objective study explores the toxicity effects and antioxidants activity properties from lichen Teloschistes flavicans isolate. The isolation of secondary metabolites was carried out by extraction, separation, and purification by means of gravity column chromatography, thin-layer chromatography, and purity tests. Toxicity activity test used the Mayer method by looking at the LC50 value, while the antioxidant activity test used the DPPH method by looking at the IC50 value. The results of the isolation obtained a needle-shaped crystal with the name of the compound is 3-[1'-(2",3"-dihydroxy-phenyl)-propyl]-7-hydroxy-chroman-4-one and the molecular formula C18H18O5. The toxicity test showed that extract and isolate compounds have LC50 values of 9.38 and 162.18 µg/mL, indicating that the isolated compound was toxic. The antioxidant test showed that the extract and isolated compounds have antioxidant activity with IC50 values of 54.05 and 127.38. The results of these studies provide scientific development of natural compounds that have the potential to act as antioxidants for diseases in the human body.

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals Antioxidant capacity profile of dewandaru leaf (extract eugenia uniflora l.): part of usadha Bali

2020 ◽  
Vol 4 (1) ◽  
pp. 87-98
Author(s):  
Puguh Santoso ◽  
Ni Luh Kade Arman Anita Dewi ◽  
Agus Adrianta
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chloroform Extract ◽  
Hexane Extract ◽  
Eugenia Uniflora ◽  
Butanol Extract ◽  
Free Radical Activity

Bali has around 50,000 usadha palm oil which is a source of untreated herbal medicine. One of the plants that have the potential as an antioxidant and is traditionally used by the community is Dewandaru (Eugenia uniflora L.) plant. Dewandaru containing tannins, flavonoids, and anthocyanins. Dewandaru plants have antibacterial activity, antioxidants, and antidotes to free radicals.  Making extract with six different solvents, among them methanol, ethyl acetate, n-butanol, chloroform, and n-hexane. The measurements were taken of the reduction of free radical activity by observing the absorbance at the maximum wavelength with a UV-Vis spectrophotometer alternately on the six samples. Secondary metabolites contained in Dewandaru leaves, namely flavonoids, tannins, and quinones. The value obtained is IC50 methanol extract 5,857 ppm; n-butanol extract 8,893 ppm; ethyl acetate extract 15,203 ppm; n-hexane extract 162.7315 ppm; and chloroform extract 75,873 ppm. Methanol extract, n-butanol, and ethyl acetate have very strong antioxidant activity because <50 ppm; n-hexane extract has weak antioxidant activity (ranging from 150-200 ppm), and chloroform extract has strong antioxidant activity (ranging from 50-100 ppm). Major conclusions: Dewandaru leaves has the potential as a natural antioxidant in the treatment section of Usadha Bali.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSRTRAK LUMUT HATI (Marchantia polymorpha)

KOVALEN ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 315-321
Author(s):  
Nurhaeni Nurhaeni ◽  
Gladys ◽  
Jaya Hardi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Hexane Extract ◽  
Marchantia Polymorpha ◽  
Dpph Method ◽  
Ethanol Fraction

The research is about antioxidant activity of the liverworts (Marchatia polymorpha) extract based on the level of a polar solvent. This research was done by maceration of liverworts by using n-hexane, ethyl acetate and ethanol solvent. The n-hexane, ethyl acetate, and ethanol fraction were tested the antioxidant activity by using DPPH method. The result was obtained for antioxidant activity (IC50) on n-hexane extract, ethyl acetate, ethanol, and vitamin C is 1065.33 ppm, 1326.52 ppm, 1876.11 ppm, and 13.45 ppm, respectively. The liverworts extract was identified contains secondary metabolites compounds of flavonoid, tannins, polyphenol, alkaloid, and steroid. Keywords: Liverwort extract, antioxidant, IC50

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