scholarly journals EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG

2020 ◽  
Vol 8 (1) ◽  
pp. 69-80
Author(s):  
Mario H.Cantona ◽  
Maxs Urias Ebenheizer Sanam ◽  
Tri Utami ◽  
Tarsisius Considus Tophianong ◽  
Antin Y.N Widi
Keyword(s):  
Antibody Titer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Serum Samples ◽  
Average Value ◽  
Antibody Titers ◽  
Random Manner ◽  
Bali Cattle ◽  
Effect Of Age ◽  
Selection Of

Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of  SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.

scholarly journals An enzyme-linked immunosorbent assay for platelet compatibility testing

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 744-749 ◽  
Author(s):  
JD Tamerius ◽  
JG Curd ◽  
P Tani ◽  
R McMillan
Keyword(s):  
Antibody Titer ◽  
Immunosorbent Assay ◽  
Clinical Laboratory ◽  
Clinical Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Volunteer Donor ◽  
Antibody Titers ◽  
Test Serum ◽  
Selection Of

Abstract The selection of platelet donors for patients who are refractory to random donor platelets often presents a difficult clinical problem. We describe an enzyme-linked immunosorbent assay (ELISA) for evaluating alloantibodies in refractory patients. Platelets from prospective donors are immobilized on microtiter plates and, after incubation with test serum and washing, platelet-bound IgG is detected with enzyme- linked anti-human IgG. Platelets from 46 prospective donors were tested. Twenty-two were judged compatible (reciprocal of the antibody titer less than 16) and, of these, 15 were used as platelet donors; each gave a measurable platelet increment after transfusion. The magnitude of the response was roughly proportional to the assay results. Platelets from donors giving antibody titers less than 4 resulted in platelet increments at 1 hr ranging from 4,890 to 22,200 (median 12,600), while platelets from donors giving titers of 8 or 16 resulted in lesser increments (550–4548). Conversely, 5 of the 24 patients found incompatible by the assay (titer greater than 16) gave no platelet increment, and in 3 instances, the recipient developed fever and chills after the transfusion. The assay is sensitive, simple, and adaptable to the clinical laboratory. Platelets from volunteer donor panels can be plated and stored for up to 6 mo.

scholarly journals Seroprevalensi Penyakit Egg Drop Syndrome pada Itik di Desa Tumbak Bayuh, Kecamatan Mengwi, Badung, Bali

2019 ◽  
Vol 37 (2) ◽  
pp. 248
Author(s):  
Gusti Ayu Yuniati Kencana ◽  
I Made Kardena ◽  
Ni Wayan Apsari Shantika Pratistha
Keyword(s):  
Veterinary Medicine ◽  
Antibody Titer ◽  
Newcastle Disease ◽  
Test Results ◽  
Serum Samples ◽  
Protective Antibody ◽  
Serological Examination ◽  
Positive Serum ◽  
Antibody Titers

Egg Drop Syndrome can cause detrimental impacts on breeders due to reducing production and quality of theaffected eggs.This study aim was to determine the seroprevalece ofantibody against Egg Drop Syndrome (EDS) virus in ducks. Antibody titers examination was done from 75blood samplesof ducks thathave not been vaccinatedagainstEDS virus. The duck samples were collected from Tumbak Bayuh Village, Mengwi, Badung. Serological examination was held at the Virology Laboratory,Faculty of Veterinary Medicine, Udayana University by usingHaemagglutination Inhibition (HI) test. HI test results showed that 24 samples were positive contained antibody of the EDS,while 51 samples were negative. Range of EDS antibody titer observed was from24 to 27 HI units.  This results indicates that the ducks have protective antibody titer against EDS virus. The positive serum samples were also tested using HI test against Newcastle Disease (ND) virus with negative result. It can be concluded that the ducks in Tumbak Bayuh Village, Mengwi, Badung have 32% of antibody titer against EDS virus whichmight result from being exposed by EDS virus naturally.

scholarly journals An enzyme-linked immunosorbent assay for platelet compatibility testing

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 744-749
Author(s):  
JD Tamerius ◽  
JG Curd ◽  
P Tani ◽  
R McMillan
Keyword(s):  
Antibody Titer ◽  
Immunosorbent Assay ◽  
Clinical Laboratory ◽  
Clinical Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Volunteer Donor ◽  
Antibody Titers ◽  
Test Serum ◽  
Selection Of

The selection of platelet donors for patients who are refractory to random donor platelets often presents a difficult clinical problem. We describe an enzyme-linked immunosorbent assay (ELISA) for evaluating alloantibodies in refractory patients. Platelets from prospective donors are immobilized on microtiter plates and, after incubation with test serum and washing, platelet-bound IgG is detected with enzyme- linked anti-human IgG. Platelets from 46 prospective donors were tested. Twenty-two were judged compatible (reciprocal of the antibody titer less than 16) and, of these, 15 were used as platelet donors; each gave a measurable platelet increment after transfusion. The magnitude of the response was roughly proportional to the assay results. Platelets from donors giving antibody titers less than 4 resulted in platelet increments at 1 hr ranging from 4,890 to 22,200 (median 12,600), while platelets from donors giving titers of 8 or 16 resulted in lesser increments (550–4548). Conversely, 5 of the 24 patients found incompatible by the assay (titer greater than 16) gave no platelet increment, and in 3 instances, the recipient developed fever and chills after the transfusion. The assay is sensitive, simple, and adaptable to the clinical laboratory. Platelets from volunteer donor panels can be plated and stored for up to 6 mo.

scholarly journals Serological Evaluation of Specific-Antibody Levels in Patients Treated for Chronic Chagas' Disease

2007 ◽  
Vol 15 (2) ◽  
pp. 297-302 ◽  
Author(s):  
Olga Sánchez Negrette ◽  
Fernando J. Sánchez Valdéz ◽  
Carlos D. Lacunza ◽  
María Fernanda García Bustos ◽  
María Celia Mora ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Treatment Effectiveness ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serological Tests ◽  
Recombinant Antigens ◽  
Antibody Titers ◽  
Laboratory Procedures ◽  
The Individual ◽  
Antibody Levels

ABSTRACT Serological tests are the main laboratory procedures used for diagnosis during the indeterminate and chronic stages of Chagas' disease. A serological regression to negativity is the main criterion used to define parasitological cure in treated patients. The aim of this work was to monitor the individual specificities of antibody levels for 3 years posttreatment in 18 adult patients. Conventional serological techniques (hemagglutination assays and enzyme-linked immunosorbent assay [ELISA]) were modified by using recombinant antigens to detect early markers of treatment effectiveness. For this purpose, serum samples were taken before and during treatment and every 6 months after treatment for at least 3 years. When hemagglutination assays were used, a decrease in antibody levels was observed in only one patient. When ELISA with serum dilutions was used, antibody clearance became much more apparent: in 77.7% (14/18) of the patients, antibody titers became negative with time. This was observed at serum dilutions of 1/320 and occurred between the 6th and the 30th months posttreatment. The immune response and the interval for a serological regression to negativity were different for each patient. For some of the recombinant antigens, only 50% (9/18) of the patients reached the serological regression to negativity. Recombinant antigen 13 might be a good marker of treatment effectiveness, since 66.6% (six of nine) of the patients presented with an early regression to negativity for specific antibodies to this antigen (P = 0.002).

scholarly journals Detection of avian reovirus antibodies in layer birds of small scale commercial farms in Dinajpur district of Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 612-621 ◽  
Author(s):  
Abdus Salam ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Farzana Afroz
Keyword(s):  
Antibody Titer ◽  
Specific Antibody ◽  
Age Groups ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Avian Reovirus ◽  
Serum Samples ◽  
Aged Group ◽  
Test Kit

The present study was conducted on layer birds of different age groups to determine specific antibody titer level against avian reovirus (ARV) by indirect enzyme linked immunosorbent assay (iELISA) at Dinajpur district of Bangladesh. This study showed that ARV specific antibody positive cases were 84 out of 90 blood serum samples and the highest antibody titer was 26120 and lowest antibody titer was 288. The total 93.33% sera samples were showed positive result. The study showed that 100% sera sample were positive against ARV at 6 weeks of aged group and the highest, lowest and mean antibody titer were 13917, 4895 and 10269 respectively. On the other hand 88.88% sera sample were positive against ARV at 10 weeks of aged group and the highest, lowest and mean antibody titer were 9779, 288 and 5689.89 respectively. The sera sample collected from 14 weeks of aged group showed 88.88% positive and the highest, lowest and mean antibody titer were 11727, 871 and 5250 respectively. The sera sample collected from 18 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 24440, 1234 and 12648.89 respectively. The sera sample collected from 22 weeks of aged group were 100% positive against ARV and the highest, lowest and mean antibody titer were 26120, 1752 and 11373.89 respectively. The sera sample collected from 26 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 8566, 1630 and 4327.44 respectively. The sera sample collected from 30 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 13431, 1989 and 5890.56 respectively. The sera sample collected from 40 weeks of aged group showed 77.77% positive against ARV and the highest, lowest and mean antibody titer were 14618, 433 and 5103.22 respectively. The sera sample collected from 48 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 14553, 957 and 7436.5 respectively. In conclusion it is evident that avian reovirus-specific antibody was successfully detected through commercially available avian reovirus antibody test kit (ELISA kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely ARV.Asian J. Med. Biol. Res. December 2015, 1(3): 612-621

scholarly journals Detection of bluetongue virus antibodies in sheep from Paraná, Brazil

2020 ◽  
Vol 41 (3) ◽  
pp. 879
Author(s):  
Maria Carolina Ricciardi Sbizera ◽  
Luiz Fernando Coelho da Cunha Filho ◽  
Michele Lunardi ◽  
Simone Fernanda Nedel Pertile ◽  
Thais Helena Constantino Patelli ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Animal Health ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Serum Samples ◽  
Agar Gel ◽  
Serological Tests ◽  
High Occurrence ◽  
Predominant Factor ◽  
World Organization

Bluetongue (BT) is an infectious and non-contagious disease caused by bluetongue virus (BTV) belonging to the genus Orbivirus. It is transmitted by a hematophagous vector, Culicoides sp., to ruminants, particularly to sheep, which are most susceptible to this disease. The main serological tests are agar gel immunodiffusion (AGID), which is recommended by the World Organization for Animal Health (OIE), and the competitive enzyme-linked immunosorbent assay (cELISA), which has the advantage of no cross-reaction with other orbiviruses. The aim was to compare the results of these two tests by conducting them on sera collected from sheep in the state of Paraná, Brazil. From March to October 2017, serum samples were collected from 270 sheep from 10 farms in six mesoregions of Paraná. The samples were subjected to AGID and cELISA to detect antibodies against BTV. Based on the test results, we classified the sheep as low, moderate, and high occurrence. The results demonstrated that 64.81% (175/270) of the sheep were seropositive through the cELISA test, showing a high occurrence, and 41.11% (111/270) were seropositive through the AGID test, indicating a moderate occurrence. The concordance between the tests was moderate (0.51) as determined by the Kappa coefficient. Among the studied farms, 90% (9/10) presented at least one seropositive sheep, and the number of animals tested positive by the cELISA test was higher than those by the AGID test. Favorable climate, which favors the presence and multiplication of the culicoid vector and the occurrence of infection, was the biggest predominant factor responsible for the obtained results. The low occurrence in farms with milder climate suggest that the presence of antibodies also occurs due to the low pathogenicity of circulating serotypes in the different mesoregions studied. It is concluded that BTV infection is present in the sheep herds in Paraná, and the occurrence was moderate detected by AGID test and high detected by cELISA test.

scholarly journals Bovine Immunoglobulin E Levels of Bali Cattlesin Bangli and Nusa Penida island Bali Province, Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 64
Author(s):  
Ni Ketut Suwiti ◽  
Luh Gde Surya Heryani ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Nyoman Werdi Susari ◽  
I Nengah Kerta Besung ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Commercial Enzyme ◽  
Elisa Kit ◽  
Bali Cattle ◽  
Bovine Immunoglobulin

The aim of this research was to detect identify levels of Bovine Immunoglobulin E (BoIg.E), can be used as an indicator of response immune in bali cattle.  Eighty serum samples were collected from Nusa Penida and Bangli region. Bovine Ig.E levels was measured using a commercial Enzyme Linked Immunosorbent Assay (ELISA) Kit. The data were analysis based on differences of farming characteristics andgeographic. The result of research that, of BoIg.E level of bali cattle kept in Bangli (34.16258 ?g/ml), was higher than Nusa Penida (22.26047 ?g/ml). We conclude that there was a significant effect of differences of farming characteristics and geographic conditions.

Relationship between efficacy of denileukin diftitox (Dd) and antibody development in cutaneous T-cell lymphoma (CTCL): An analysis of two phase III studies

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e19534-e19534
Author(s):  
M. Acosta ◽  
M. Duvic ◽  
A. Lopez-Anaya
Keyword(s):  
Fusion Protein ◽  
Cell Lymphoma ◽  
Phase Iii ◽  
Test Results ◽  
Spearman Correlation ◽  
Serum Samples ◽  
Two Phase ◽  
Antibody Titers ◽  
Pre Treatment ◽  
Phase Iii Studies

e19534 Background: Dd is a novel IL-2 receptor-targeted recombinant fusion protein approved for persistent/recurrent CTCL expressing CD25. The effect of antibody titers on efficacy in CTCL was assessed in 2 phase III studies (11 & 14). Methods: Dd doses were 9 or 18 mcg/kg daily for 5 days, repeating every 21 days, up to 8 courses. Serum samples were collected prior to Dd infusion on day 1 of courses 1, 3, 5, and 7. Immune response to Dd was assessed using 2 enzyme-linked immunoassays: 1 for anti-IL-2 antibody (Ab) and the other for anti-Dd Ab. Immunogenicity data reflect percentage of subjects whose test results were considered positive for antibody to the intact Dd fusion protein. Results: In Study 11, 66% of subjects tested positive for anti-Dd Ab at baseline, possibly due to prior exposure to diphtheria toxin or its vaccine. In both studies, and for both doses, rapid increases in Ab titers were observed in nearly 100% of subjects from courses 1 to 3 (∼650-fold, 11; >=625-fold, 14). After 3 treatment courses, mean Ab levels stabilized and did not change appreciably with time. Pharmacokinetic (PK) parameters (Cmax and AUC) decreased substantially during the same period, but no relationship between Ab formation and clearance was identified. Low Spearman Correlation coefficient values indicated lack of a significant association (i) between the pre-treatment or treatment-related Ab levels and (ii) between PK changes for subjects who responded and subjects who did not respond. Of subjects positive for Ab at baseline, 43% (27/63) and 38% (13/34) responded to treatment, as compared to 50% (16/32) and 54% (7/13) for subjects who had no detectable Ab at baseline in Studies 11 and 14 respectively. There appeared to be higher incidences of response in subjects with lower initial Ab titers. Conclusions: In the studies analyzed, antibody formation in response to Dd exposure did not appear to compromise efficacy for treatment of CTCL. [Table: see text]

scholarly journals Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment

2006 ◽  
Vol 14 (2) ◽  
pp. 150-156 ◽  
Author(s):  
Silvio Bertini ◽  
Arnaldo L. Colombo ◽  
Helio K. Takahashi ◽  
Anita H. Straus
Keyword(s):  
Immune Response ◽  
Paracoccidioides Brasiliensis ◽  
Immunoglobulin M ◽  
Antifungal Treatment ◽  
Primary Immune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carbohydrate Structure ◽  
Dimorphic Fungus ◽  
Serum Samples ◽  
Antibody Titers

ABSTRACT Paracoccidioidomycosis (PCM) is a granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. The immunoglobulin classes and isotypes of antibodies directed to acidic glycosphingolipids (GSLs) and glucosylceramide of P. brasiliensis were determined by enzyme-linked immunosorbent assay of sera from 31 PCM patients. The reactivities of 38 serum samples were analyzed by considering the stage of treatment: before antifungal treatment (n = 10), during 1 to 4 months of treatment (T1-4; n = 9), during 5 to 12 months of treatment (T5-12; n = 9), and posttreatment (PT; n = 10). Sera from healthy subjects (n = 12) were used as controls. Only the GSL Pb-1 antigen, which presents the carbohydrate structure Galfβ1-6(Manα1-3)Manβ1, was reactive with the PCM patient sera. The PCM patient sera did not react with Pb-2, which lacks the Galf residue and which is considered the biosynthetic precursor of Pb-1, indicating that the Galf residue is essential for antibody reactivity. The Pb-1 glycolipid from nontreated patients elicited a primary immune response with immunoglobulin M (IgM) production and subsequent switching to IgG1 production. The IgG1 titer increased after the start of antifungal treatment (T1-4 group), and general decreases in the anti-Pb-1 antibody titers were observed after 5 months of treatment (T5-12 and PT groups). The Pb-1 antigen, an acidic GSL with terminal Galf residue, has potential application as an elicitor of the host immune response in patients with PCM.

scholarly journals Development of a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay for detecting canine distemper virus

2020 ◽  
Vol 104 (24) ◽  
pp. 10725-10735
Author(s):  
Yuan Zhang ◽  
Gang Xu ◽  
Lu Zhang ◽  
Jiakai Zhao ◽  
Pinpin Ji ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Canine Distemper Virus ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Canine Distemper ◽  
Serum Samples ◽  
Ideal Method

Abstract Canine distemper virus (CDV) infection causes mass mortality in diverse carnivore species. For effective virus surveillance, rapid and sensitive assays are needed to detect CDV in field samples. In this study, after BABL/c mice were immunized with recombinant CDV-fusion (F) protein, monoclonal antibodies (mAbs) against recombinant CDV-F protein (designated 1A5, 1A6, and 7D5) were produced using traditional hybridoma cell technology. Next, capture antibody (1A6, 800 ng/well) and horseradish peroxidase (HRP)–conjugated detection antibody (HRP-7D5, 1:100, 500 ng/well) were used in a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay (ELISA) for CDV detection after optimization of both mAb amounts per well using a checkerboard titration test. Based on sandwich ELISA test results for 120 known CDV-negative samples, the cutoff value for a positive result was set to an OD450 nm value ≥ 0.196. As compared with test results obtained from commercial immune colloidal gold test strips, the low limits of detection for the two assays were revealed to be 100 TCID50 per 100 μL. In addition, the sandwich ELISA agreed 100% and 96.4% with commercial immune colloidal gold test strips when testing serum and stool samples. The sandwich ELISA assay provided statistically similar CDV detection. Thus, the sandwich ELISA developed here to detect CDV in fecal and serum samples provided good sensitivity, high specificity, and good reproducibility and should serve as an ideal method for large-scale surveillance of CDV infections in carnivores. Key points • Three CDV mAbs that recognized different epitopes and bound to virion were generated. • The sandwich ELISA based mAbs to detect CDV in fecal and serum samples was developed. • The sandwich ELISA is an ideal method for detecting CDV infections in the field.

Sign in / Sign up

Export Citation Format

Share Document