scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized from Plant Latex

2020 ◽  
pp. 1579-1588
Author(s):  
Ghada Mohammed Saleh ◽  
Shaymaa Suhail Najim
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Medical Field ◽  
Gram Negative ◽  
Force Microscopy ◽  
Industrial Project ◽  
Atomic Force ◽  
Diffusion Assay ◽  
Plant Latex

     Nanoparticles produced by plants are preferred in the medical field for its safe and unpolluted product; it is also accepted as an ecofriendly, non-expensive, and non-toxic nanomaterial. In this study, silver nitrate was successfully used to produce silver nanoparticles (AgNPs) by the use extractsof 4 different latex-producing plants which belong to 2 families (Moraceae and Euphorbiaceae). The synthesis was proved by Atomic Force Microscopy (AFM).The sizes of the AgNP grains were estimated by Granularity Cumulating Distribution (GCD). The results revealed the production of AgNPs in different sizes of 103 and 82 nm using the Moraceae family and 77 and74nm using the Euphorbiaceae family.Antibacterial activity was also detected against both Gram positive and Gram negative pathogenic bacteria using the well diffusion assay. In conclusion, this source of nanoparticles can be a very useful industrial project in a goal to find new safe and economic alternatives to antibiotics.

scholarly journals Green Synthesis Concept of Nanoparticles From Environmental Bacteria and Their Effects on Pathogenic Bacteria

2020 ◽  
pp. 1289-1297
Author(s):  
Ghada Mohammed Saleh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Soil Bacteria ◽  
Pathogenic Bacteria ◽  
Pseudomonas Sp ◽  
Good Source ◽  
Force Microscopy ◽  
Atomic Force ◽  
Environmental Bacteria ◽  
Inhibition Zones

Soil bacteria play an interesting role in the reduction of Ag+ ions and the formation of silver nanoparticles (AgNPs), which may be a good source for nanoparticles and play a major role in nanotechnology applications. The concept of this project was to study the effects of these environmentally produced nanoparticles on the growth of some pathogenic bacteria. The environmental bacteria were isolated from soil, purified on broth cultures, and centrifuged, while the supernatant was extracted to detect its ability to convert silver nitrate to nanoparticles. The AgNPs was detected by Atomic Force Microscopy (AFM), while Granularity Cumulating Distribution (GCD) was employed to estimate the AgNPs sizes. The results showed the synthesis of AgNPs with sizes of 63.50nm and 45.81nm from the extracts of environmental Pseudomonas sp. and Enterobacter, respectively. The synthesized AgNPs from the extracts of all environmental bacteria showed antibacterial activity against some pathogenic bacteria (Gram positive and Negative) with variable inhibition zones. In conclusion, environmental bacteria can be a cheap source of nanoparticles.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

scholarly journals Enhancement in the antibacterial activity of cephalexin by its delivery through star-shaped poly(ε-caprolactone)-block-poly(ethylene oxide) coated silver nanoparticles

2020 ◽  
Vol 7 (10) ◽  
pp. 201097
Author(s):  
Sana Rahim ◽  
Samina Perveen ◽  
Shakil Ahmed ◽  
Muhammad Raza Shah ◽  
Muhammad Imran Malik
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Ethylene Oxide ◽  
Inhibitory Concentration ◽  
Bacterial Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biofilm Destruction ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

The antibacterial activity of silver nanoparticles (AgNPs) stabilized with a four-armed star-shaped poly(ε-caprolactone)-block-poly(ethylene oxide) copolymer [St-P(CL-b-EO)] and its application as a drug delivery vehicle for cephalexin (Cp) was evaluated against pathogenic Staphylococcus aureus . The prepared AgNPs were characterized by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, zeta sizer and atomic force microscopy (AFM). The antibacterial efficiency of Cp is enhanced several-fold by its delivery through complexation with St-P(CL-b-EO)-AgNPs, monitored by microplate assay and biofilm destruction studies. Finally, the visual destruction of bacterial cells and its biofilms by employing Cp and its conjugates at their minimum inhibitory concentration (MIC 50 ) and minimum biofilm inhibitory concentration (MBIC 50 ), respectively, is observed by topographic imaging by AFM. Enhanced antibacterial activity of St-P(CL-b-EO)-AgNPs loaded Cp is attributed to penetrative nature of the drug cargo St-P(CL-b-EO)-AgNPs towards the bacterial cell wall.

scholarly journals Green Sonochemical Synthesis of Silver Nanoparticles Using Adansonia Digitata Leaves Extract and Evaluation of Their Antibacterial Potential

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Wilson Mbiti Njue ◽  
Jackson Kilonzo Kithokoi ◽  
Jane Mburu ◽  
Henry Mwangi ◽  
Sauda Swaleh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Metal Nanoparticles ◽  
Pathogenic Bacteria ◽  
Cost Effective ◽  
Adansonia Digitata ◽  
Gram Negative ◽  
Selected Area Electron Diffraction ◽  
Average Size

Metal nanoparticles in the field of nanotechnology are of great interest to modern scientific research due to their size effects, medical uses and, catalytic, electronic and optical properties. Green synthesis of metal nanoparticles is a feasible alternative to chemical methods as it is environmentally friendly and cost effective. In continuation with our research on green synthesis of silver nanoparticles using Kenyan medicinal plants, we here report the synthesis of novel silver nanoparticles (AgNPs) on ultrasonic bath using Adansonia digitata leaves extracts and analysis of their antibacterial activity. The nanoparticles were characterized by UV-Vis, High Resolution Transmission Electron Microscopy (HRTEM), FTIR spectroscopy and Energy Dispersive X-ray (EDX). EDX analysis affirmed the nanoparticles were pure silver. Crystalline nature of the nanoparticles was confirmed by bright circular spots in the Selected Area Electron Diffraction (SAED) in HRTEM image. The AgNPs were spherical with an average size 13 nm. FTIR analysis showed strong –C=C- and –OH stretching bands due to compounds capping the nanoparticles. The synthesized AgNPs showed high inhibition zones of 17.1±0.130 mm towards Gram-negative bacteria E. coli and 12.9±0.082mm towards Gram positive bacteria S. aureus. The aqueous A. digitata extract had no effect on growth inhibition of test bacteria. The study showed that the silver nanoparticles synthesized from the plant’s leaves extract had antibacterial activity against both Gram negative and positive pathogenic bacteria. The nanoparticles can be utilized towards developing novel drugs useful in combating pathogens.

scholarly journals Biological Activity and Nanostructuration of Fe3O4-Ag/High Density Polyethylene Nanocomposites

2019 ◽  
Vol 3 (2) ◽  
pp. 34 ◽  
Author(s):  
Phuong Nguyen-Tri ◽  
Van Nguyen ◽  
Tuan Nguyen
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
High Density Polyethylene ◽  
High Density ◽  
Hybrid Nanoparticles ◽  
Hybrid Nanostructure ◽  
Ag Nps ◽  
Force Microscopy ◽  
Atomic Force ◽  
Growth Method

We report here the synthesis of uniform nanospheres-like silver nanoparticles (Ag NPs, 5–10 nm) and the dumbbell-like Fe3O4-Ag hybrid nanoparticles (FeAg NPs, 8–16 nm) by the use of a seeding growth method in the presence of oleic acid (OA)/oleylamine (OLA) as surfactants. The antibacterial activity of pure nanoparticles and nanocomposites by monitoring the bacterial lag–log growth has been investigated. The electron transfer from Ag NPs to Fe3O4 NPs which enhances the biological of silver nanoparticles has been proven by nanoscale Raman spectroscopy. The lamellae structure in the spherulite of FeAg NPs/High Density Polyethylene (HDPE) nanocomposites seems to play the key role in the antibacterial activity of nanocomposites, which has been proven by nanoscale AFM-IR. An atomic force microscopy coupled with nanoscale infrared microscopy (AFM-IR) is used to highlight the distribution of nanoparticles on the surface of nanocomposite at the nanoscale. The presence of FeAg NPs in PE nanocomposites has a better antibacterial activity than that reinforced by Ag NPs due to the faster Ag+ release rate from the Fe3O4-Ag hybrid nanoparticles and the ionization of Ag NPs in hybrid nanostructure.

scholarly journals Enhancement of Antibacterial Activity of Capped Silver Nanoparticles in Combination with Antibiotics, on Model Gram-Negative and Gram-Positive Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Aruna Jyothi Kora ◽  
Lori Rastogi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Sodium Dodecyl ◽  
Average Particle ◽  
Gram Positive ◽  
Yellow Colour ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Xrd Patterns ◽  
Diffusion Assay

The nanoparticles used in this study were prepared from AgNO3using NaBH4in the presence of capping agents such as citrate, sodium dodecyl sulfate, and polyvinylpyrrolidone. The formed nanoparticles were characterized with UV-Vis, TEM, and XRD. The generation of silver nanoparticles was confirmed from the appearance of yellow colour and an absorption maximum between 399 and 404 nm. The produced nanoparticles were found to be spherical in shape and polydisperse. For citrate, SDS, and PVP capped nanoparticles, the average particle sizes were38.3±13.5,19.3±6.0, and16.0±4.8 nm, respectively. The crystallinity of the nanoparticles in FCC structure is confirmed from the SAED and XRD patterns. Also, the combined antibacterial activity of these differently capped nanoparticles with selected antibiotics (streptomycin, ampicillin, and tetracycline) was evaluated on model Gram-negative and Gram-positive bacteria, employing disc diffusion assay. The activity of the tested antibiotics was enhanced in combination with all the stabilized nanoparticles, against both the Gram classes of bacteria. The combined effects of silver nanoparticles and antibiotics were more prominent with PVP capped nanoparticles as compared to citrate and SDS capped ones. The results of this study demonstrate potential therapeutic applications of silver nanoparticles in combination with antibiotics.

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

scholarly journals Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Royal Jelly ◽  
Fluorescence Emission ◽  
Spherical Particles ◽  
Ag Nps ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

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