scholarly journals PENGARUH NAA DAN IBA TERHADAP PERAKARAN PURWOCENG (Pimpinella pruatjan Molk.) 77V VITRO

2020 ◽  
Vol 11 (4) ◽  
pp. 146
Author(s):  
SITTIFATMAH SYAHID ◽  
OTIH ROSTIANA ◽  
MIFTAKHUROHMAH MIFTAKHUROHMAH
Keyword(s):  
Tissue Culture ◽  
Root Induction ◽  
Planting Material ◽  
Randomized Design ◽  
Induction Technique ◽  
Medicinal Crops ◽  
Culture Performance ◽  
Completely Randomized Design ◽  
Plant Acclimatization

<p>ABSTRAK<br />Purwoceng (Pimpinella pruatjan Molk.) merupakan tanaman obat<br />langka yang cukup potensial untuk dikembangkan sebagai bahan baku<br />afrodisiak. Untuk mendukung budidaya tanaman ini diperlukan bahan<br />tanaman yang memadai. Perbanyakan in vitro purwoceng untuk<br />memperoleh bahan tanaman secara masal masih dibatasi oleh sulitnya<br />menginduksi akar, yang berakibat rendahnya keberhasilan aklimatisasi di<br />lapangan. Penelitian bertujuan untuk mendapatkan teknik induksi<br />perakaran dengan menggunakan dua jenis auksin (NAA dan IBA) pada<br />berbagai taraf konsentrasi yaitu 0; 0,1; 0,2; 0,4; 0,6; 0,8; 1,0; 1,5 dan 2,0<br />mg/1. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, Balai<br />Penelitian Tanaman Rempah dan Obat, Bogor dari bulan Januari 2003<br />sarnpai dengan Februari 2004. Rancangan percobaan yang digunakan<br />adalah acak lengkap dengan tiga ulangan. Setiap ulangan terdiri dari tiga<br />tunas. Parameter yang diamati adalah jumlah akar, panjang akar dan<br />jumlah daun layu serta penampakan kultur secara visual. Hasil penelitian<br />menunjukkan bahwa auksin NAA nyata menghasilkan jumlah akar lebih<br />banyak dan lebih panjang dari IBA. Penggunaan NAA 0,8 g/1 merupakan<br />konsentrasi terbaik untuk induksi akar. Tidak ada perbedaan yang nyata<br />dari penggunaan NAA atapun IBAterhadap parameter jumlah daun layu.<br />Kata kunci : Purwoceng, Pimpinella pruatjan Molk, tanaman obat,<br />pengatur tuumbuh , NAA, IBA, induksi akar, in vitro, Bogor</p><p><br />ABSTRACT<br />Effect of NAA and IBA on root induction of pruatjan<br />(Pimpinella pruatjan Molk.) in vitro<br />Pruatjan (Pimpinella pruatjan Molk.) is one of endangered species<br />which is potential to be developed as aphrodisiac source. To support<br />pruatjan cultivation , it is needed to prepare the planting material. In vitro<br />propagation of pruatjan is hampered by the difficulty in inducing the<br />normal roots which affect the successful plant acclimatization. The<br />objective of this research was to obtain the root induction technique using<br />two kinds of auxin (NAA and IBA ) at several concentrations i.e : 0; 0.1;<br />0.2; 0.4; 0.6; 0.8; 1.0; 1.5 and 2.0 mg/1. This experiment was conducted<br />from January 2003 to February 2004 at the Tissue Culture Laboratory of<br />Indonesian Spices and Medicinal Crops Research Institute (ISMECRI) in<br />Bogor. Experiment was designed as a completely randomized design with<br />three replications. Each replication consisted of three shoots. The<br />parameters observed were number of roots, length of roots, number of<br />senessence leaves and culture performance. The result showed that NAA<br />produced the greatest and the longest roots compared to that of DBA. The<br />use of NAA 0,8 mg/1 performed the best treatment to induce roots. The<br />number of senessence leaves was neither affected by NAA nor IBA.<br />Key words: Pruatjan, Pimpinella pruatjan Molk., medicinal plant,<br />growth regulator, NAA, IBA, root induction, in vitro</p>

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals Optimization of in vitro rooting protocol for tomato (Lycopersicon esculentum mill.) varieties

2020 ◽  
Vol 12 (3) ◽  
pp. 365-371
Author(s):  
Endalkachew Baye ◽  
Temesgen Matewos ◽  
Derbew Belew
Keyword(s):  
Lycopersicon Esculentum ◽  
Root Length ◽  
In Vitro Rooting ◽  
Root Induction ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill ◽  
Randomized Design ◽  
Optimized Protocol ◽  
Completely Randomized Design

In vitro rooting of micropropagated shoots were carried out with the aim of evaluating the root induction responses of two tomatoes (Lycopersicon esculentum MILL) varieties (Gelilema and Chali) using Indole -3- butyric acid (IBA). Seven levels of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg L-1) were used in a completely randomized design (CRD) in factorial combinations (seven level of IBA*two varieties) with three replications.  After the plantlets were kept in the rooting media for three weeks, data on rooting percentage, number of roots/shoots and root length in cm were collected. The analysis of variance showed that the interaction of IBA*Var was highly significantly different for rooting percentage, a number of roots/shoot and root length at p<0.01. The highest rooting percentage (100.00±0.00), number of roots/shoot (14.20±0.35) and root length (10.7±0.29) were received from Chali on free Murashige and Skoog medium (MS). At the same time, the lowest percentage of rooting (11.11±0.00), number of roots/shoot (0.887±0.19) and root length (1.00±0.00 cm) were obtained from Gelilema on MS+1.5mg/l IBA. For acclimatization, the in vitro rooted shoots were transplanted into plastic pots containing a mixture of oven sterilized soil and sand at a ratio of 2:1. After three weeks, a survival rate of 67.7% for Chali and 58.1% for Gelilema was obtained. From the above result, it can be concluded that free MS medium was the best for in vitro rooting of the two tomato varieties. The optimized protocol will be useful for rapid in vitro multiplication of the two tomato varieties.

scholarly journals Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽  
10.15575/1344 ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 97-109
Author(s):  
Lamro Purba ◽  
Erni Suminar ◽  
Denny Sobardini ◽  
Wieny Rizky ◽  
Syariful Mubarok
Keyword(s):  
Tissue Culture ◽  
Leaf Number ◽  
Shoot Induction ◽  
Seed Technology ◽  
Randomized Design ◽  
Rooting Media ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fast Time ◽  
Endogenous Auxin ◽  
Seed Technology ◽  
Randomized Design ◽  
Process Plants ◽  
Number Of Leaves ◽  
Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

scholarly journals In Vitro Screening Ketahanan Galur Padi (Oryza Sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Keracunan Besi (Fe)

2019 ◽  
Vol 19 (3) ◽  
pp. 241
Author(s):  
Onny Chrisna Pandu Pradana ◽  
Siti Novridha Andini
Keyword(s):  
Tissue Culture ◽  
Oryza Sativa ◽  
Iron Toxicity ◽  
In Vitro Screening ◽  
Culture Bottle ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
And Control

This research aimed to invstigate the response of paddy culture (B7 strain) assembled by Lampung State Polytechnic to the iron toxicity tolerance. The research was done at Plant Tissue Culture Laboratory, Lampung State Polytechnic, from July to September 2019. Treatments were single arranged in a completely randomized design with three replications. The treatment tried was five levels of Fe concentrations (5,6 ppm 28 ppm, 56 ppm, 84 ppm, 112 ppm, and control). Each replication consisted of three culture bottle containing one explant. The homogenity of data was tested using Barlett test. If the assumption were fulfilled then analysis of variance is executed using STATISTIX 10, and then followed by the Honest Significant Difference (HSD) test in 5% alpha for mean separation and RPA analysis. The result of this research showed that the B7 strain has tolerance to iron toxicity until 56 ppm of Fe concentration, it can be concluded from the PAR value of its strain (>0,50). Meanwhile in  84 and 112 ppm of Fe concentration, the RPA value of B7 starin (<0,50), and it is indicate that its strain is sensitive. 

RESPONSE OF TURMERIC SHOOT EXPLANT AFTER CYTOKININ WITH IN VITRO

2019 ◽  
Vol 24 (2) ◽  
pp. 143-152
Author(s):  
Marhan Nurullia ◽  
Erni Suminar Suminar ◽  
Anne Nurani
Keyword(s):  
Tissue Culture ◽  
Root Length ◽  
Air Flow ◽  
T Test ◽  
Shoot Height ◽  
Best Response ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Laminar Air Flow

This study was aimed at determining the response of turmeric shoot explants after the provision of various types and concentrations of cytokinins in vitro. This experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University from January to April 2018. The data were analyzed using T-Test. The experimental method used in this research was Completely Randomized Design (CRD). Explant planting was carried out in Laminar Air Flow. The experiment consisted of 7 treatments consisting of 4 replications and each test consisted of 4 units. Observation of this experiment was carried out for 12 MST. The main observations were made on the data that were tested statistically namely the percentage of explant growing shoots, percentage of explant growing roots, shoot height, number of tuns, number of roots and root length. The treatments consisted of Control, 2.5 mg L-1 BAP, 5 mg L-1 BAP, 0.5 mg L-1 TDZ, 1 mg L-1 TDZ, 0.01 mg L-1 Zeatin and 0.1 mg L Zeatin -1. The results show that the treatment of 1 mg L-1 TDZ shows the best response to the growth of turmeric explants by increasing the number of turmeric shoot explants than the others.RESPONS EKSPLAN TUNAS KUNYIT SETELAH SITOKININ SECARA IN VITROTujuan dari penelitian ini yaitu untuk melihat respons eksplan tunas kunyit terhadap pemberian berbagai jenis dan konsentrasi sitokinin secara in vitro. Percobaan ini dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Padjadjaran dari bulan Januari sampai April 2018. Hasil percobaan dianalisis dengan Sample T-Test. Metode percobaan yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL). Penanaman eksplan dilakukan di dalam Laminar Air Flow. Percobaan terdiri dari 7 perlakuan sebanyak 4 ulangan dan setiap ulangan terdiri dari 4 unit. Pengamatan percobaan ini dilakukan selama 12 MST. Pengamatan utama dilakukan terhadap data-data yang diuji secara statistik yakni persentase eksplan tumbuh tunas, persentse eksplan tumbuh akar, tinggi tunas, jumlah tunas, jumlah akar dan panjang akar. Perlakuan terdiri dari Kontrol; 2,5 mg L-1 BAP; 5 mg L-1 BAP; 0,5 mg L-1 TDZ; 1 mg L-1 TDZ; 0,01 mg L-1 Zeatin; dan 0,1 mg L-1 Zeatin. Hasil penelitian menunjukkan bahwa perlakuan 1 mg L-1 TDZ menunjukkan respons yang lebih baik terhadap pertumbuhan eksplan kunyit dengan meningkatkan jumlah tunas eksplan tanaman kunyit daripada yang lainnya.

scholarly journals INDUKSI DAN REGENERASI KALUS KELADI TIKUS (Typonium flagelliforme. Lodd. ) SECARA IN VITRO

2020 ◽  
Vol 13 (4) ◽  
pp. 142 ◽  
Author(s):  
SITTI FATIMAH SYAHID ◽  
NATALINI NOVA KRISTINA
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Carbon Source ◽  
Genetic Variability ◽  
In Vitro Culture ◽  
Basic Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Group B

<p>ABSTRAK<br />Keladi tikus umumnya diperbanyak secara vegetatif sehingga ragam<br />genetiknya sempit. Penelitian peningkatan keragaman genetik pada keladi<br />tikus melalui kultur in vitro telah dilakukan di Laboratorium Kultur<br />Jaringan, Balai Penelitian Tanaman Obat dan Aromatik (Balittro) Bogor<br />pada bulan April sampai Desember 2005. Bahan tanaman yang digunakan<br />adalah daun steril keladi tikus in vitro. Media dasar yang digunakan adalah<br />Murashige and Skoog (MS) yang diperkaya vitamin dari group B. Sebagai<br />sumber energi digunakan sukrosa sebanyak 30 g/l. Penelitian terdiri dari<br />dua tahap yaitu induksi dan regenerasi kalus. Perlakuan yang diuji pada<br />tahap I adalah beberapa taraf konsentrasi auksin (2,4-D) secara tunggal<br />maupun kombinasi dengan sitokinin (kinetin) terhadap induksi kalus yaitu<br />: 2,4-D 0,1 mg/l; 2,4-D 0,5 mg/l; 2,4-D 1,0 mg/l; 2,4-D 0,1 + kinetin 0,1<br />mg/l; 2,4-D 0,5 mg/l + kinetin 0,1 mg/l; 2,4-D 1.0 mg/l + kinetin 0,1 mg/l;<br />2,4-D 0,1 mg/l + kinetin 0,3 mg/l; 2,4-D 0,5 mg/l +kinetin 0,3 mg/l dan<br />2,4-D 1,0 mg/l + kinetin 0,3 mg/l. Tahap II adalah beberapa taraf<br />konsentrasi benzyl adenin untuk regenerasi kalus. Penelitian disusun<br />menggunakan rancangan acak lengkap dengan pola faktorial dan lima<br />ulangan, dan setiap ulangan terdiri dari satu eksplan. Faktor pertama<br />adalah asal kalus dan faktor kedua adalah beberapa taraf konsentrasi BA<br />yaitu : BA 0,1 mg/l ; BA 0,3 mg/l dan BA 0,5 mg/l. Parameter yang<br />diamati adalah waktu inisiasi kalus, struktur dan warna kalus, jumlah<br />tunas serta penampilan kultur secara visual. Hasil penelitian menunjukkan<br />bahwa kalus asal eksplan daun dapat diinduksi pada perlakuan 2,4-D 1,0<br />mg/l + kinetin 0,1 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l dengan<br />waktu inisiasi 8 sampai 10 minggu setelah perlakuan. Regenerasi kalus<br />terbaik diperoleh pada medium 2,4-D 1,0 mg/l + kinetin 0,3 mg/l<br />mengandung BA 0,3 mg/l dengan rata-rata tunas dan daun yang dihasilkan<br />sebanyak 13,2 tunas dan 4,4 daun.<br />Kata kunci : Keladi tikus, Typonium flagelliforme Lodd., induksi,<br />regenerasi kalus, in vitro</p><p><br />ABSTRACT<br />Induction and regeneration of Rodent tuber calli through<br />in vitro culture<br />Rodent tuber plant (Typonium flagelliforme Lodd) is commonly<br />propagated vegetatively, the repro its genetic variation is narrow. A<br />research to increase the genetic variability of the plant was conducted in<br />Tissue Culture Laboratory of the Indonesian Medicinal and Aromatic<br />Research Institute, Bogor from April to December 2005. The leaf of<br />Rodent tuber in vitro used as an explants. Murashige and Skoog (MS)<br />medium used as basic medium, supplemented with vitamin from B group,<br />sucrose 30 g/l was added into the medium as carbon source. The research<br />consist of two steps : 1) calli induction and 2) calli regeneration. The<br />treatment tested in first step : 2.4-D 0.1 mg/l; 2.4-D 0.5 mg/l; 2.4-D 1,0<br />mg/l; 2.4-D 0.1 + kinetin 0.1 mg/l; 2.4-D 0.5 mg/l + kinetin 0.1 mg/l; 2.4-<br />D 1.0 mg/l + kinetin 0,1 mg/l; 2.4-D 0.1 mg/l + kinetin 0.3 mg/l; 2.4-D 0.5<br />mg/l + kinetin 0.3 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l. In the<br />second steps, several concentration of BA were tested i.e: BA 0,1 mg/l ;<br />BA 0,3 mg/l and BA 0,5 mg/l. The experiment was arranged in<br />completely randomized design with factorial pattern. Each treatment<br />consist of five replications. The parameters observed were time of calli<br />initiation, texture, colour of calli and number of shoot and leaves in<br />regeneration. The result showed that calli can be induced on 2.4-D 1.0<br />mg/l + kinetin 0.1 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l, eight to ten<br />weeks after culture. The best medium for shoots regeneration contains 2.4-<br />D 1.0 mg/l + kinetin 0.3 mg/l with 0.3 mg/l BA, with mean result of 13.2<br />shoots and 4.4 leaves.<br />Key words : Rodent tuber, Typonium flagelliforme Lodd. bl , induction,<br />regeneration, calli, in vitro</p>

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