scholarly journals Kemampuan Bakteri Endofit dalam Mengendalikan Penyakit Rebah Kecambah dan Layu Sclerotium (Sclerotium rolfsii) pada Kedelai

2017 ◽  
Vol 1 (2) ◽  
pp. 52
Author(s):  
Husda Marwan
Keyword(s):  
Disease Severity ◽  
Endophytic Bacteria ◽  
Seed Treatment ◽  
Sclerotium Rolfsii ◽  
Soybean Seed ◽  
Wilt Disease ◽  
Healthy Plant ◽  
Diseased Plant ◽  
Damping Off

Endophytic bacteria have potency as candidates of biological control agents to plant disease, because the bacteria as reported to be associated with plant resistance to pathogens. The study aimed to determine the ability of endophytic bacteria to inhibit the growth of Sclerotium rolfsii in vitro, and suppress damping off disease incedence and Sclerotium wilt disease severity on soybean. The ability of endophytic bacteria to inhibit S. rolfsii was carried out on 44 isolates that isolated from soybean, rice and banana. The ability of endophytic bacteria to control damping off disease was carried out on 13 isolates were selected in vitro (BE-CW1, BE-M1, BE-M2, BE-M3, BE-M4, BE-S1, BE-S6, BE-S13, BE-S14, BE -BRRG2, BE-KH1), and also fungicides and two controls (healthy plant and diseased plant). The ability of endophytic bacteria to control Sclerotium wilt was carried out on 6 isolates which showed pressure on damping off disease (BE-M1, BE-M2, BE-M3, BE-M4, BE-CW1, BE-KH2) and 2 controls (healthy plants and diseased plants).The results showed that the thirteen isolates of endophytic bacteria could inhibit mycelial growth and germination S. rolfsii. Soybean seed treatment revealed that dipping the seed in endophytic bacterial isolates suppressed damping off disease incedence with a range of 37.4 to 49.9% and Sclerotium wilt disease severity of 20.1 to 85 % , as well as reduced the mortality of soybean by 26.3 to 84.4 % . BE-KH2 isolate was able to suppress Sclerotium wilt in soybean by 85% and reduce the mortality by 67.5%.

Endophytic Bacteria of Cotton and Sweet Corn for Providing Growth Promotion and Biological Disease Control

1996 ◽  
Author(s):  
Joseph W. Kloepper ◽  
Ilan Chet
Keyword(s):  
Seed Treatment ◽  
Protein Production ◽  
Sweet Corn ◽  
Growth Promotion ◽  
Sclerotium Rolfsii ◽  
Chitinolytic Enzymes ◽  
Low Concentrations ◽  
Bacillus Spp ◽  
Biological Control Potential

Endophytes were isolated from 16.7% of surface-disinfested seeds and 100% of stems and roots of field-growth plants. Strains from Israel with broad-spectrum in vitro antibiosis were mainly Bacillus spp., and some were chitinolytic. Following dipping of cut cotton roots into suspensions of these strains, endophytes were detected up to 72 days later by isolation and by autoradiograms of 14C-labelled bacteria. Selected endophytes exhibited biological control potential based on significant reductions in disease severity on cotton inoculated with Rhizoctonia solani or Fusarium oxysporum f. sp. vasinfectum as well as control of Sclerotium rolfsii on bean. Neither salicylic acid nor chitinase levels increased in plants as a result of endophytic colonization, suggesting that the observed biocontrol was not accounted for by PR protein production. Some biocontrol endophytes secreted chitinolytic enzymes. Model endophytic strains inoculated into cotton stems via stem injection showed only limited movement within the stem. When introduced into stems at low concentrations, endophytes increased in population density at the injection site. After examining several experimental and semi-practical inoculation systems, seed treatment was selected as an efficient way to reintroduce most endophytes into plants.

scholarly journals The Efficacy of Ethaboxam as a Soybean Seed Treatment Toward Phytophthora, Phytopythium, and Pythium in Ohio

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1421-1432 ◽  
Author(s):  
Kelsey Scott ◽  
Meredith Eyre ◽  
Dair McDuffee ◽  
Anne E. Dorrance
Keyword(s):  
Seed Treatment ◽  
Soybean Seed ◽  
Primary Objective ◽  
Phytophthora Species ◽  
Damping Off ◽  
Seed Treatments ◽  
Seedling Disease ◽  
Seed Decay ◽  
Fungicide Mixtures ◽  
Seed Rot

Phytophthora, Phytopythium, and Pythium species that cause early-season seed decay and pre-emergence and post-emergence damping off of soybean are most commonly managed with seed treatments. The phenylamide fungicides metalaxyl and mefenoxam, and ethaboxam are effective toward some but not all species. The primary objective of this study was to evaluate the efficacy of ethaboxam in fungicide mixtures and compare those with other fungicides as seed treatments to protect soybean against Pythium, Phytopythium, and Phytophthora species in both high-disease field environments and laboratory seed plate assays. The second objective was to evaluate these seed treatment mixtures on cultivars that have varying levels and combinations of resistance to these soilborne pathogens. Five of eight environments received adequate precipitation in the 14 days after planting for high levels of seedling disease development and treatment evaluations. Three environments had significantly greater stands, and three had significantly greater yield when ethaboxam was used in the seed treatment mixture compared with treatments containing metalaxyl or mefenoxam alone. Three fungicide formulations significantly reduced disease severity compared with nontreated in the seed plate assay for 17 species. However, the combination of ethaboxam plus metalaxyl in a mixture was more effective than either fungicide alone against some Pythium and Phytopythium species. Overall, our results indicate that the addition of ethaboxam to a fungicide seed treatment is effective in reducing seed rot caused by these pathogens commonly isolated from soybean in Ohio but that these effects can be masked when cultivars with resistance are planted.

Biological control of damping-off of sugar-beet and cotton with Chaetomium globosum or a fluorescent Pseudomonas sp.

1988 ◽  
Vol 34 (5) ◽  
pp. 631-637 ◽  
Author(s):  
D. Walther ◽  
D. Gindrat
Keyword(s):  
Biological Control ◽  
Sugar Beet ◽  
Seed Treatment ◽  
Pythium Ultimum ◽  
Chaetomium Globosum ◽  
Pseudomonas Sp ◽  
Damping Off ◽  
Antifungal Metabolites ◽  
Fluorescent Pseudomonas

Seed treatment with ascospores of Chaetomium globosum reduced damping-off of sugar-beet caused by seed-borne Phoma betae and soil-borne Pythium ultimum or Rhizoctonia solani in growth chamber experiments. Seed treatment with a fluorescent Pseudomonas sp. controlled Ph. betae and P. ultimum but not R. solani. Coating cotton seeds with ascospores controlled P. ultimum and R. solani damping-off. In some experiments, biological seed treatments were equally or more effective than seed treatment with captan. However, greater variability in disease control occurred with the antagonists than with captan. Fifty percent of freshly harvested ascospores of C. globosum germinated in 8 h on water agar. When ascospores were stored under air-dried conditions for 3 days to 2.5 years, germination increased to > 90%. Under same storage conditions, survival of Pseudomonas sp. was detected after 4 months. Antagonistic activities observed in vitro were hyphal coiling of C. globosum on R. solani, and mycostasis was induced by C. globosum or Pseudomonas sp. on agar and soil. The presumed cause of mycostasis is the diffusible antifungal metabolites which may also be involved in the biological control of damping-off.

scholarly journals Biological control of Ralstonia solanacearum (Smith), the causal pathogen of bacterial wilt disease by using Pantoea spp

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Sabry A. Hassan
Keyword(s):  
Biological Control ◽  
Cell Suspension ◽  
Disease Severity ◽  
Culture Filtrate ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Nucleotide Sequence Analysis ◽  
Tomato Root

Abstract Background Bacterial wilt of tomato (BWP) caused by Ralstonia solanacearum (Smith) is a very important disease. Biological control of this disease is a very important tool to protect the plant and environment from pollution of chemical control. Results Twenty isolates of genus, Pantoea were isolated from healthy tomato root. Out of 20 isolates, 2 strains, PHYTPO1 and PHYTPO2, showed highly antagonistic property to control the growth of R. solanacearum in vitro conditions. They were identified as P. agglomerans by using 16S rRNA nucleotide sequence analysis. The 2 isolates were selected to study their effect (as cell suspension or culture filtrate) on the bacterial wilt under greenhouse conditions. PHYTPO1 inhibited maximum growth reduction of R. solanacearum and formed 2.5 cm2 of inhibition zone, followed by 1.2 cm2 in PHYTOPO2 under in vitro conditions. Treating with both isolates of P. agglomerans was significantly reduced disease severity of tomato wilt disease. The disease severity was reduced to 74.1 when treated as cell suspension, while when treated as culture filtrate, it reduced the disease severity up to 69.4 than infected control. Conclusion The strains of Pantoea can be used as an ecofriendly method to control of the most economic pathogen of tomato under greenhouse conditions. Further study is needed to find an appropriate formulation and approving application of these bacteria under field conditions.

scholarly journals Efektivitas Limbah Cengkih dalam Menekan Perkembangan In Vitro Sclerotium rolfsii, Jamur Penyebab Damping Off Kacang Tanah

2021 ◽  
Vol 17 (1) ◽  
pp. 36-42
Author(s):  
Handry R D Amanupunyo ◽  
Nace E Tahitu ◽  
Gratiana N C Tuhumury
Keyword(s):  
Sclerotium Rolfsii ◽  
Plant Diseases ◽  
Damping Off ◽  
Flower Stalk ◽  
Colony Diameter ◽  
Randomized Design ◽  
Leaf Powder ◽  
Peanut Production ◽  
Completely Randomized Design

Various problems have caused the decline in peanut production, among others the low resistance of peanut plants to various diseases. One of the obstacles in peanut production is the attack of plant diseases, such as the fungus Sclerotium rolfsii Sacc. The use of plant-based insecticides can be an alternative to disease control in peanuts. This study aimed to obtain the appropriate and effective dose of clove leaves and flower stalks in suppressing the in vitro growth of the fungus S. rolfsii Sacc causing damping off in peanuts, and to test the effectiveness of clove leaves and flower stalks on the formation of sklerotia of the fungus. This study used ‘tuni’ clove powder from Waai Village, Salahutu District. This experimental study employed a completely randomized design (CRD) with 8 treatments and 3 replications. The results showed that the growth of the fungus S. rolfsii Sacc. was inhibited by clove flower stalk powder at a dose of 1.5 g per100 mL of PDA, which was indicated by the number of sklerotia formed of 13.67% and propagule weight of 0.12 g, followed by a dose of clove leaf powder of 1.5 g per100 mL PDA, with amount of sklerotia formed of 27.33% and propagule weight of 0.17 g. These results were statistically shown on the parameters: colony diameter, Sklerotia formation time, number of Sklerotia, and weight of propagules. Keywords: Clove powder, dosage, in vitro, Sclerotium rolfsii Sacc.   ABSTRAK Berbagai masalah telah menyebabkan turunnya produksi kacang tanah, diantaranya masih rendahnya ketahanan tanaman kacang tanah terhadap berbagai penyakit. Salah satu kendala dalam produksi kacang tanah adalah serangan penyakit tanaman, seperti jamur Sclerotium rolfsii Sacc. Penggunaan insektisida nabati dapat menjadi alternatif pengendalian penyakit pada kacang tanah. Penelitian ini bertujuan untuk memperoleh dosis daun dan tangkai bunga cengkih yang tepat dan efektif dalam menekan pertumbuhan jamur S. rolfsii Sacc penyebab damping off pada kacang tanah secara in vitro, serta menguji efektifitas daun dan tangkai bunga cengkih terhadap pembentukan sklerotia jamur. Penelitian ini menggunakan bubuk cengkih tuni, dari Desa Waai, Kecamatan Salahutu. Penelitian eksperimental ini menggunakan Rancangan Acak Lengkap (RAL) dengan 8 perlakuan dan 3 ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan jamur S. rolfsii Sacc. terhambat oleh bubuk tangkai bunga cengkih pada dosis 1,5 g per 100 mL PDA yang ditunjukan oleh jumlah sklerotia terbentuk 13,67% dan berat propagul 0,12 g dan diikuti oleh dosis bubuk daun cengkih 1,5 g per 100 mL PDA, dengan jumlah sklerotia 27,33% dan berat propagul 0,17 g. Hasil ini secara statistik ditunjukkan pada parameter: diameter koloni, waktu terbentuk sklerotia, jumlah sklerotia, dan berat propagul. Kata kunci: bubuk cengkih, dosis, in vitro, Sclerotium rolfsii Sacc.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT DARI TANAMAN PADI (Oryza sativa) SEBAGAI PENGENDALI PENYAKIT HAWAR DAUN BAKTERI AKIBAT Xanthomonas oryzae pv. oryzae

2018 ◽  
Vol 12 (1) ◽  
pp. 18-26
Author(s):  
Army Dita Serdani ◽  
Luqman Qurata Aini ◽  
Abdul Latief Abadi
Keyword(s):  
Oryza Sativa ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Xanthomonas Oryzae ◽  
Healthy Plant ◽  
Plant Tissues ◽  
Molecular Method ◽  
Corynebacterium Sp ◽  
Rice Tissues

Rice cultivation often face obstacles, and one of them is bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). The application of endophytic bacteria is one of solutions to overcome this problem. Endophytic bacteria are non-pathogenic bacteria, which live in plant tissues. These bacteria could be isolated from the plant tissues. They may adapt to the plant tissues and produce antibiosis that could increase the plant resistance. Therefore, objectives of the research were to isolate the endophytic bacteria from healthy plant tissues and to identify them through morphology, physiology, biochemistry, and molecular. Method of the research was explorative along with three principal activities, such as isolation, selection, and identification on the potential endophytic bacteria. The isolation of the endophytic bacteria from healthy rice tissues has resulted 53 isolates and five of them have antagonistic ability in vitro against Xoo. Isolate ak9 has the highest antagonistic ability, 7.67 mm, in comparison with other isolates. Results of identification showed that those five potential bacteria have close relations, such as da3 with Bacillus cereus, isolate ak9 with Burkholderia sp., isolate ak30 with Enterobacter sp and isolate da9, as well as ak15 are Corynebacterium sp.

scholarly journals Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

2020 ◽  
pp. 25-31
Author(s):  
Mahbuba Kaniz Hasna ◽  
Md. Abul Kashem ◽  
Farid Ahmed
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Disease Incidence ◽  
Sclerotium Rolfsii ◽  
Antagonistic Activity ◽  
Root Weight ◽  
Dual Culture ◽  
Collar Rot ◽  
Rot Disease

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment. 

scholarly journals Fungicide Seed Treatment Effects on Seedling Damping-off of Pumpkin Caused by Phytophthora capsici

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 63-68 ◽  
Author(s):  
M. Babadoost ◽  
S. Z. Islam
Keyword(s):  
Seed Germination ◽  
Untreated Control ◽  
Mycelial Growth ◽  
Seed Treatment ◽  
Culture Medium ◽  
Phytophthora Capsici ◽  
Field Trials ◽  
Seedling Vigor ◽  
Damping Off

Apron XL LS (mefenoxam) and Allegiance FL (metalaxyl) were highly inhibitory to growth of mycelium of Phytophthora capsici in vitro. Effective dose (ED50) of mefenoxam and metalaxyl for 50% inhibition of mycelial growth, for all five isolates of P. capsici tested, was 0.98 and 0.99 μg a.i./ml of culture medium, respectively. For mefenoxam at 200 μg a.i./ml, sporangium and zoospore germination were reduced by 92 and 96%, respectively, and 21 and 24%, respectively, for metalaxyl. In greenhouse studies, seed treatment with mefenoxam (0.42 ml of Apron XL LS/kg of seed) and metalaxyl (0.98 ml of Allegiance FL/kg of seed) significantly reduced pre- and post-emergence damping-off of seedlings caused by P. capsici in three pumpkin cultivars (Dickinson, Hybrid-401, and Hybrid-698) tested. Thirty-one days after seeding, at inoculum levels of 0, 90, 600, 1,400, and 4,000 CFU/g of soil, the average seedling stands for mefenoxam treatment were 98.4, 93.8, 88.3, 77.8, and 64.8%; for metalaxyl, 99.1, 85.3, 85.8, 73.5, and 59.3; and for the untreated control, 97.5, 55.2, 45.7, 37.0, and 22.9%, respectively. In field trials, the average seedling stands 35 days after seeding were 76.7, 74.7, and 44.9% for mefenoxam, metalaxyl, and untreated control, respectively. Seed treatment with mefenoxam or metalaxyl did not have any significant effect on either seed germination or seedling vigor.

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