TTC test on individual milk samples

In this research work the concentration of zinc, copper, lead, chromium, cadmium, and nickel in cow milk samples obtained from four different grazing areas (kakuri, kudendan, malali, kawo) of Kaduna metropolis. The samples were digested by wet digestion technique .The trace element were determined using bulk scientific model VPG 210 model Atomic Absorption Spectrophotometer (AAS).. The concentration of the determined heavy metal were The result revealed that Cr, Ni and Cd were not detected in milk samples from Kawo, Malali and Kudendan whereas lead (Pb) is detected in all samples and found to be above the stipulated limits of recommended dietary allowance (NRC,1989) given as 0.02mg/day. Cu and Zn are essential elements needed by the body for proper metabolism and as such their deficiency or excess is very dangerous for human health. However, they were found in all samples and are within the recommended limits while Cd (2.13 – 3.15 mg/kg) in milk samples from Kakuri was found to be above such limit (0.5mg/day). Cow milk samples analyzed for heavy metals in this research work pose a threat of lead and cadmium toxicity due to their exposure to direct sources of air, water and plants in these grazing areas, thereby, resulting to a potential health risk to the consumers.

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Relationships among macro-minerals, other selected serum markers of bone profile and milk components of dairy cows during late-lactation

Annals of Animal Science ◽

10.2478/aoas-2020-0094 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Ryszard Mordak ◽

Zbigniew Dobrzański ◽

Robert Kupczyński

Keyword(s):

Dairy Cows ◽

Milk Fat ◽

Dry Matter ◽

Inorganic Phosphorus ◽

Serum Markers ◽

Laboratory Diagnostics ◽

Lactation Period ◽

Milk Samples ◽

Milk Components ◽

Macro Mineral

AbstractTesting blood and milk parameters as well as analysing the relationships among these markers is very useful for monitoring the internal homeostasis and health in high-yielding dairy cows during various production periods. The aim of the study was to assess the correlations (relationships) among macro-minerals, such as calcium (Ca), inorganic phosphorus (P), magnesium (Mg), other selected bone profile markers, such as total protein (TP), albumin, activity of alkaline phosphatase (ALP) measured in serum and selected milk components such as number of somatic cells (SCC), colony-forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), dry matter (DM), non-fat dry matter (FDM) and milk production in late-lactation cows. Both blood and milk samples were collected from 11 clinically healthy milking cows during the late-lactation period. The cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples for laboratory and statistical analysis. Significant correlations were observed between: Mg and MP, Mg and FDM, ALP and SCC, TP and SCC, TP and MP, TP and FDM, albumin and MP, albumin and FDM, P and Mg, Mg and albumin, and between TP and albumin. When monitoring macro-mineral homeostasis and mammary gland health, especially in intensively fed high-yielding dairy cows correlations between these markers should be considered. The revealed correlations can allow for deeper comparative laboratory diagnostics of homeostasis and can be especially useful for laboratory monitoring of the potential risk of subclinical macro-mineral deficiency in high-yielding dairy cows.

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Immunodetection of Ostertagia ostertagi in Cattle Milk Samples in Wasit Province

Journal of Education College Wasit University ◽

10.31185/eduj.vol1.iss28.29 ◽

2017 ◽

Vol 1 (28) ◽

pp. 637-648

Author(s):

Abbas Hassan Khlaty Al-Sray

Keyword(s):

Milk Production ◽

Herd Size ◽

Elisa Test ◽

Production Factor ◽

Size Factor ◽

Ostertagia Ostertagi ◽

Milk Samples ◽

Management Group ◽

Breed Group ◽

Age Factor

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ³18 liters/day group, 14.29 % for ³10-18 liters/day group, and 25 % for < 10 liters/day group; in age factor, 13.41% for ³3-6 years group, and 14.75% for >6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for <25 (cow/herd) group, and 17.78% for ³ 25 (cow/herd) group. Statistically, the significant differences (P£ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.

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Technical note: Development of a closed-tube isothermal multiple self-matching-initiated amplification assay for visual detection of Staphylococcus aureus in milk samples

Journal of Dairy Science ◽

10.3168/jds.2020-19023 ◽

2020 ◽

Author(s):

Xuelong Chen ◽

Honghai Wang ◽

Chang Liu ◽

Yanping Qi ◽

Weina Guo

Keyword(s):

Staphylococcus Aureus ◽

Visual Detection ◽

Technical Note ◽

Milk Samples ◽

Closed Tube ◽

Amplification Assay

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The Effectiveness of Amoxicillin and Cefalexin in Two Species from Staphylococcus and Bacillus Isolated from Bovine Mastitis in Sudan

Journal of Regenerative Biology and Medicine ◽

10.37191/mapsci-2582-385x-1(1)-004 ◽

2019 ◽

Author(s):

Reem Rabie Mohammed Salih

Keyword(s):

Bacillus Cereus ◽

Bovine Mastitis ◽

Bacillus Coagulans ◽

Staph Aureus ◽

Milk Samples ◽

Sensitivity Tests ◽

Bacillus Spp ◽

Staphylococcus Spp

In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.

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A Novel Solid-phase Microextraction Method Coupled with HPLC for Determination of Four Sulfonamides in Milk Samples

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION) ◽

10.3724/sp.j.1096.2013.20889 ◽

2013 ◽

Vol 41 (4) ◽

pp. 529

Author(s):

Ying PENG ◽

Huan HE ◽

Cheng SUN ◽

Ya-Ling ZHANG ◽

Wen-Chao LI ◽

...

Keyword(s):

Solid Phase Microextraction ◽

Solid Phase ◽

Milk Samples

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Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

The Open Biotechnology Journal ◽

10.2174/187407070190130137 ◽

2019 ◽

Vol 13 (1) ◽

pp. 137-145 ◽

Cited By ~ 1

Author(s):

Tzonka Godjevargova ◽

Zlatina Becheva ◽

Yavor Ivanov ◽

Andrey Tchorbanov

Keyword(s):

Monoclonal Antibody ◽

Magnetic Nanoparticles ◽

Polyclonal Antibody ◽

Polyclonal Antibodies ◽

Food Poisoning ◽

Staphylococcal Enterotoxin ◽

Staphylococcal Enterotoxin A ◽

Fluorescence Immunoassay ◽

Magnetic Separator ◽

Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

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Quality Assessment Of Milk Samples Of Amreli Region With Nanotechnology Based Dipstick And Other Known Technology: A Comparative Study

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/11.2/18 ◽

2018 ◽

Vol 11 (2) ◽

pp. 313-317

Author(s):

Rakesh B Kabariya

Keyword(s):

Comparative Study ◽

Quality Assessment ◽

Milk Samples

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Biofilm Formation and Associated Genes in Listeria Monocytogenes

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i3.7079 ◽

2017 ◽

Vol 12 (3) ◽

Author(s):

S. R. Warke ◽

V. C. Ingle ◽

N. V. Kurkure ◽

P. A. Tembhurne ◽

Minakshi Prasad ◽

...

Keyword(s):

Public Health ◽

Listeria Monocytogenes ◽

Multiplex Pcr ◽

Food Processing ◽

Biofilm Formation ◽

Milk Samples ◽

Biofilm Production ◽

Food Borne ◽

Serious Infections ◽

Microtiter Assay

Listeria monocytogenes, an opportunistic food borne pathogen can cause serious infections in immunocompromised individuals. L. monocytogenes is capable of producing biofilm on the surface of food processing lines and instruments.The biofilm transfers contamination to food products and impose risk to public health. In the present study biofilm producing ability of L. monocytogenes isolates were investigated phenotypically and genotypically by microtiter assay and multiplex PCR, respectively. Out of 38 L. monocytogenes isolates 14 were recovered from animal clinical cases, 12 bovine environment and 12 from milk samples. A total of 3 (21.42%) clinical, 2 (16.66%) environment and 3 (25%) milk samples respectively, revealed biofilm production in microtiter assay. Cumulative results showed that 23 (60.52%) out of 38 strains of L. monocytogenes were positive for luxS and flaA gene and 1 (2.63%) was positive only for the flaA gene.

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