PHEROMONES FROM MALE DOMESTIC MICE INFLUENCES THE SEXUAL FUNCTION AND GONADAL STEROIDS LEVELS OF FEMALE LABORATORY RATS

It is well established that the behavior of different mammalians, including rodents, may undergo profound changes in the presence of individuals of other species. Therefore, the aim of this study was to verify if the presence of pheromones from male mice could influence the reproductive parameters of female rats. Twenty Swiss (SW) male mice and 16 Long Evans (LE) female Rats were housed in separate rooms, with communication in the ceiling in its entire length. A continuous flow of air was planned to pass through the room of the mice before the room of the rats and finally be exchanged with the external environment. During 45 days, vaginal smears were collected. Relative weights of reproductive organs, estradiol, progesterone, and dehydroepiandrosterone levels were also measured. The total duration of the estrous cycle and relative organ weight remained unchanged. On the other hand, the proestrus and estrus phases, as well as the estradiol levels were increased, while the diestrus phase was significantly reduced. The results have shown that maintaining LE female rats in the presence of pheromones from male SW mice can significantly change the sexual function and gonadal steroid levels.

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PHYSIOLOGICAL EFFECTS OF THE PINEAL GLAND IN ANDROGEN-STERILIZED FEMALE RATS

Acta Endocrinologica ◽

10.1530/acta.0.0630667 ◽

1970 ◽

Vol 63 (4) ◽

pp. 667-678 ◽

Cited By ~ 4

Author(s):

Russel J. Reiter

Keyword(s):

Pineal Gland ◽

Reproductive Organs ◽

Female Rats ◽

Inhibitory Influence ◽

Light Deprivation ◽

Vaginal Smears ◽

Adult Rats ◽

Androgen Treatment ◽

Postnatal Treatment ◽

Cervical Ganglia

ABSTRACT The influence of early androgen treatment, light deprivation (by blinding), pinealectomy and superior cervical ganglionectomy on the reproductive system of female rats was tested. Early postnatal treatment of rats with testosterone propionate caused adult rats to exhibit the characteristic signs of androgen sterilization; these included polyfollicular ovaries, normal-sized uteri and persistent vaginal cornification. If early androgentreated rats also were blinded the ovaries were smaller in size and contained fewer follicles, the uteri were greatly reduced in size and the incidence of vaginal oestrus was decreased by approximately 50% If in addition to blinding, androgen-sterilized animals were subjected to either removal of the pineal gland or superior cervical ganglia, the reproductive organs and the vaginal smears were indistinguishable from those of testosterone-treated rats with eyes. These data indicate that the inhibitory influence of blinding on the pituitary-ovarian axis was mediated through the sympathetic nervous system and the pineal gland. The restraining influence of light deprivation on the growth of the reproductive organs was not permanent as illustrated by the fact that if these animals were kept to 120 days of age the ovaries and uteri grew to the same level as those of pinealectomized control rats.

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Effect of methanolic extract of Amaranthus viridis leaves on reproductive functions in wistar female rats

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i6-s.3775 ◽

2019 ◽

Vol 9 (6-s) ◽

pp. 119-126

Author(s):

Mataphouet Emmanuel AFFY ◽

Wahon Marie-Odile TOVI ◽

N’guessan Ernest ZOUGROU ◽

Koffi KOUAKOU

Keyword(s):

Methanolic Extract ◽

Day Treatment ◽

Total Duration ◽

Female Rats ◽

Histological Structure ◽

Female Rat ◽

Pharmacological Effects ◽

Vaginal Smears ◽

Estrogenic Potential ◽

Significant Difference

Objective : The aim of this study is to determine the pharmacological effects and the estrogenic properties of Amaranthus viridis leaves on the reproductive function of animal model (female rat). Methods : Vaginal smears performed 9 days before treatment allowed to select female rats having alternated on two cycles a regularity. Thereafter, the selected rats were administered by gavage daily for 28 days taking care of smear every morning at 7am from the first day of treatment follow the evolution of the cycle. For this study 20 nulliparous rats, 2 months old, weighing between 120-150 g. The first group (control) was administered with olive oil and the other three batches received respectively the doses 200, 400 and 600 mg/kg of the methanolic extract of Amaranthus viridis. At the end of the 28-day treatment, ovary and uterine horn were removed, histological and hormonal parameters were studied for determine pharmacological effects of methanolic extract of Amaranthus viridis. Results : The extract caused a disturbances of the cycle according to the doses administered. Disturbances at doses 200 and 400 mg/kg PC are significant. The calculation of the total duration of the different phases of the cycle revealed very significant increases in the estrous phase (P<0.01) by 22.79 % and 17.13 % at the respective doses of 200 and 400 mg/kg b.w compared to control. Non-significant difference was recorded on FSH, LH and estradiol level. On progesterone level, administration of the methanolic extract showed a significant difference at dose of 600 mg/kg b.w compared to control. On histological structure of the ovary, the presence of active and degenerate corpus luteum, secondary follicles depending on the dose administered were recored. Conclusion : The results showed that the methanolic extract of Amaranthus viridis contain estrogenic substances or estrogen-like substances according to a dose-dependent mechanism, with high estrogenic potential at doses of 200 and 400 mg/kg b.w . Keywords: vaginal smears, Amaranthus viridis, methanolic extract, histology

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DEVELOPMENT OF FEMALE RATS INJECTED SHORTLY AFTER BIRTH WITH TESTOSTERONE OR »ANABOLIC STEROIDS«

Acta Endocrinologica ◽

10.1530/acta.0.0450402 ◽

1964 ◽

Vol 45 (3) ◽

pp. 402-414 ◽

Cited By ~ 11

Author(s):

Dora Jacobsohn

Keyword(s):

Ovarian Function ◽

Testosterone Propionate ◽

Anabolic Steroids ◽

Newborn Infants ◽

Reproductive Organs ◽

Female Rats ◽

Corpora Lutea ◽

List Type ◽

Vaginal Smears ◽

Early Effects

ABSTRACT The present work is concerned with early effects of a single injection of 1.5 mg testosterone propionate into 5 day old female rats (injected rats). Effects of »anabolic steroids« given to rats shortly after birth, generally on the 5th day, were also studied. Untreated littermates served as controls. As early as 5 days after injection (rat's age 10 days) a reduction in ovarian weight was noticeable. At 10 days after injection the ovaries weighed less than those of controls. Vaginal smears showing cornified cells were obtained from injected rats about 5 days prior to controls. At the controls' first oestrous the ovaries of the injected rats weighed approximately 60 per cent of those of the controls. At this time the ovaries of the controls contained large follicles but no corpora lutea. Confirming previous results of other workers, injected rats observed for about 2 months presented constant vaginal oestrous and ovaries without corpora lutea. The ovaries weighed less than half those of the controls. This result was obtained irrespective of whether one ovary had been removed a fortnight previously, or not. Rats given 1.5 mg testosterone propionate at 20 days of age did not show any persistent effects. Records of weight of body, pituitary gland and adrenals were also in agreement with results of other investigators. Results obtained from similar experiments with »anabolic steroids« (19-nor-testosterone, 1-methyl-androst-1-enolone-oenanthate and methan-drostenolone = Durabol, Primobolan and Dianabol, respectively) indicated that, in the doses studied so far, a depression of endocrine activities and/or persistent defects in the development of female accessory reproductive organs and ovarian function may occur in rats after an injection given within 24 hours after birth or at 5 days of age. The importance of further investigations with regard to the clinical use of »anabolic steroids«, particularly in newborn infants, is discussed.

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Pelvic Impairments

Guides Newsletter ◽

10.1001/amaguidesnewsletters.2018.julaug02 ◽

2018 ◽

Vol 23 (4) ◽

pp. 9-10

Author(s):

James Talmage ◽

Jay Blaisdell

Keyword(s):

Pelvic Ring ◽

Reproductive Organs ◽

High Impact ◽

Pelvic Fractures ◽

Motor Dysfunction ◽

Impact Event ◽

Concomitant Injuries ◽

Rating Process ◽

Pass Through ◽

A Minor

Abstract Pelvic fractures are relatively uncommon, and in workers’ compensation most pelvic fractures are the result of an acute, high-impact event such as a fall from a roof or an automobile collision. A person with osteoporosis may sustain a pelvic fracture from a lower-impact injury such as a minor fall. Further, major parts of the bladder, bowel, reproductive organs, nerves, and blood vessels pass through the pelvic ring, and traumatic pelvic fractures that result from a high-impact event often coincide with damaged organs, significant bleeding, and sensory and motor dysfunction. Following are the steps in the rating process: 1) assign the diagnosis and impairment class for the pelvis; 2) assign the functional history, physical examination, and clinical studies grade modifiers; and 3) apply the net adjustment formula. Because pelvic fractures are so uncommon, raters may be less familiar with the rating process for these types of injuries. The diagnosis-based methodology for rating pelvic fractures is consistent with the process used to rate other musculoskeletal impairments. Evaluators must base the rating on reliable data when the patient is at maximum medical impairment and must assess possible impairment from concomitant injuries.

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Effect of Exposure to Mixture of Heavy Metals Arsenic, Cadmium and Lead on Reproductive Function and Oxidative Stress in Female Rats

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i03.9075 ◽

2017 ◽

Vol 9 (03) ◽

Author(s):

Choudhuri D. ◽

Bhattacharjee T.

Keyword(s):

Oxidative Stress ◽

Heavy Metals ◽

Ascorbic Acid ◽

Reproductive Function ◽

Reproductive Organs ◽

Female Rats ◽

Corpora Lutea ◽

Oestrus Cycle ◽

Implantation Sites ◽

Live Fetus

Background : Toxicological consequences arising from exposure to mixtures of heavy metals especially at low, chronic and environmentally relevant doses are poorly recognised. In the present study, we evaluated effects of chronic exposure to combinations of three metals arsenic (As), cadmium (Cd) and lead (Pb) present frequently in drinking water on reproductive function and oxidative damage caused to reproductive organs of female rats. Method : Female rats were exposed to mixture of metals (As, Cdand Pb) for 90 consecutive days. The gain in body weight and weight of reproductive organs were recorded following autopsy on 91 stday. The oestrus cycle were monitored during entire treatment period. Numbers of corpora lutea, implantation sites, live fetus and survival of the fetus were evaluated in rats mated successfully with untreated male after completion of their respective treatment. Ovarian cholesterol, protein, ascorbic acid and enzyme Δ 5 -3β HSD levels were estimated. Serum levels of steroid hormones oestrogen and progesterone were estimated. Histopathological picture of both ovary and uterus were assessed. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPX) activity, amount of reduced glutathione (GSH) and malondyaldehyde (MDA) in blood, ovary and uterus were measured as biomarkers of oxidative stress. Results : The treated rats showed reduced body weight gain and reduction in the weight of ovary and uterus. Oestrus cycle was disrupted with continuous diestrous in treated animals. Number of corpora lutea, implantation sites and live fetus and the survival of fetus evaluate were reduced significantly in treated groups. The levels of ovarian cholesterol and ascorbic acid increased in treated rats with decrease Δ5 -3β HSD level. There was reduction in serum level of both the ovarian steroid hormones oestrogen and progesterone. The protein levels did not differ between the groups. There was a significant increase in levels of MDA and decrease in levels of all the antioxidant enzymes in treated group. Conclusion : The results revealed there was disruption to reproductive functions with decrease in stereoidogenic activity and associated oxidative stress in female rats treated with combination of mixture of metals (Cd, As and Pb) at low dose for 90 consecutive days.

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Longitudinal Melatonin Production in Female Laboratory Rats During 1997-2006: Possible Modulatory Effects of Changing Solar Activity

Current Aging Science ◽

10.2174/1874609811205030004 ◽

2013 ◽

Vol 5 (3) ◽

pp. 186-194 ◽

Cited By ~ 3

Author(s):

Hella Bartsch ◽

Dieter Mecke ◽

Hansgeorg Probst ◽

Heinz Kupper ◽

Eckhard Seebald ◽

...

Keyword(s):

Solar Activity ◽

Laboratory Rats ◽

Melatonin Production ◽

Female Laboratory

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Comparative effects of Orchis anatolica vs. the red Korean Panax ginseng treatments on testicular structure and function of adult male mice

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2012-0035 ◽

2015 ◽

Vol 12 (1) ◽

Author(s):

Nabil A. Khouri ◽

Haytham M. Daradka ◽

Mohammed Z. Allouh ◽

Ahmad S. Alkofahi

Keyword(s):

Panax Ginseng ◽

Male Fertility ◽

Virgin Female ◽

Reproductive Organs ◽

Serum Markers ◽

Structure And Function ◽

Control Group ◽

Male Mice ◽

Fertility Potential ◽

And Function

Abstract: The effects of: Both plants were administered orally to two separate mice groups at a dose of 800 mg/kg/day for 35 days and compared with control group. After treatment, 5 mice of each group were sacrificed and total mice weights, reproductive organs’ weights, spermatogenesis, and androgenic serum markers were investigated. The remaining mice from all groups were allowed to mate with virgin female mice to explore male fertility potential.: Results indicated that body and organs’ weights were increased significantly in mice treated with: We can conclude that

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Kindlin-2 in Sertoli cells is essential for testis development and male fertility in mice

Cell Death and Disease ◽

10.1038/s41419-021-03885-4 ◽

2021 ◽

Vol 12 (6) ◽

Author(s):

Xiaochun Chi ◽

Weiwei Luo ◽

Jiagui Song ◽

Bing Li ◽

Tiantian Su ◽

...

Keyword(s):

Sertoli Cells ◽

Male Fertility ◽

Nuclear Translocation ◽

Hippo Pathway ◽

Reproductive Organs ◽

Male Reproduction ◽

Barrier Structure ◽

Male Mice ◽

Sperm Development

AbstractKindlin-2 is known to play important roles in the development of mesoderm-derived tissues including myocardium, smooth muscle, cartilage and blood vessels. However, nothing is known for the role of Kindlin-2 in mesoderm-derived reproductive organs. Here, we report that loss of Kindlin-2 in Sertoli cells caused severe testis hypoplasia, abnormal germ cell development and complete infertility in male mice. Functionally, loss of Kindlin-2 inhibits proliferation, increases apoptosis, impairs phagocytosis in Sertoli cells and destroyed the integration of blood-testis barrier structure in testes. Mechanistically, Kindlin-2 interacts with LATS1 and YAP, the key components of Hippo pathway. Kindlin-2 impedes LATS1 interaction with YAP, and depletion of Kindlin-2 enhances LATS1 interaction with YAP, increases YAP phosphorylation and decreases its nuclear translocation. For clinical relevance, lower Kindlin-2 expression and decreased nucleus localization of YAP was found in SCOS patients. Collectively, we demonstrated that Kindlin-2 in Sertoli cells is essential for sperm development and male reproduction.

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Neospora caninum (Protozoa: Apicomplexa) infections in rats

Canadian Journal of Zoology ◽

10.1139/z90-236 ◽

1990 ◽

Vol 68 (7) ◽

pp. 1595-1599 ◽

Cited By ~ 12

Author(s):

David S. Lindsay ◽

J. P. Dubey

Keyword(s):

Rattus Norvegicus ◽

Neospora Caninum ◽

Clinical Signs ◽

Hepatic Necrosis ◽

Female Rats ◽

Methylprednisolone Acetate ◽

Laboratory Rats ◽

Experimental Inoculation ◽

Caninum Tachyzoites ◽

Subcutaneous Inoculation

The susceptibility of laboratory rats (Rattus norvegicus) to experimental inoculation with tachyzoites of Neospora caninum was examined. Groups of female rats were intramuscularly injected with 0, 2, or 4 mg of methylprednisolone acetate (MPA) 7 days prior to, and on the day of, subcutaneous inoculation with 0 or 1.5 × 105 tachyzoites. Clinical signs of disease or deaths did not occur in rats given nothing or only N. caninum tachyzoites. Rats given only 4 mg MPA failed to grow as well as rats given nothing or only N. caninum tachyzoites but were otherwise healthy. All of 20 rats given 4 mg MPA and tachyzoites died of hepatitis and pneumonia within 12 days postinoculation. Hepatic necrosis was the most striking lesion seen in these rats, and other milder lesions consisted of pneumonia, encephalitis, and myositis. The response of rats given 2 mg MPA and tachyzoites was less severe. Three of 20 rats died with encephalitis, myositis, hepatitis, and pancreatitis. Mild lesions, but no N. caninum tachyzoites, were seen in 3 of 14 rats inoculated only with tachyzoites. Rats given the 4 mg MPA treatment and inoculated with N. caninum tachyzoites appear to be suitable subjects for examining acute neosporosis and could be used in studies designed to examine treatment of acute disease.

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