scholarly journals The Standardization of Aronia melanocarpa Fruits Growing on the Territory of Orenburg Region

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Shmygareva A. A. ◽  
Sankov A. N. ◽  
Dudarenkova M. R. ◽  
Kochukova A. A. ◽  
Dorokhina O. A. ◽  
...  
Keyword(s):  
Ethyl Alcohol ◽  
Extraction Time ◽  
Relative Degree ◽  
Confidence Probability ◽  
Total Flavonoids ◽  
Orenburg Region ◽  
Aronia Melanocarpa ◽  
Analytical Wavelength ◽  
Direct Spectrophotometry

For standardization of Aronia melanocarpa fruits the method of direct spectrophotometry at analytical wavelength 534 nm was used1 . The optimal extraction conditions of flavonoids from fruits of Aronia melanocarpa Elliot. - extractant is 96% ethyl alcohol; the ratio of "raw-extractant" - 1:30; extraction time -30 min1 . The relative degree of the determination of the total flavonoids in fruits of Aronia melanocarpa Elliot. in used method with confidence probability 0,95 is no more than ±3,64%. The content of total flavonoids in fruits of Aronia melanocarpa Elliot. varied from 1,6% to 7,5% (calculated on cyanidin-3-O-glikozid).

scholarly journals The Standardization of Crataegus sanguinea Fruits Growing on the Territory of Orenburg Region

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Shmygareva A. A. ◽  
Sankov A. N. ◽  
Dudarenkova M. R. ◽  
Kochukova A. A. ◽  
Dorokhina O. A. ◽  
...  
Keyword(s):  
Ethyl Alcohol ◽  
Extraction Time ◽  
Relative Degree ◽  
Confidence Probability ◽  
Total Flavonoids ◽  
Orenburg Region ◽  
Differential Spectrophotometry ◽  
Analytical Wavelength

For standardization of Crataegus sanguinea fruits the method of differential spectrophotometry at analytical wavelength 412 nm was used1. The optimal extraction conditions of flavonoids from fruits of Crataegus sanguinea Pall. – extractant is 70% ethyl alcohol; the ratio of “raw-extractant” – 1:30; extraction time -60 min1. The relative degree of the determination of the total flavonoids in fruits of Crataegus sanguinea Pall. in used method with confidence probability 0,95 is no more than ±3,5%. The content of total flavonoids in fruits of Crataegus sanguinea Pall. varied from 0,18±0,001% to 0,22±0,002% (calculated on hyperoside).

scholarly journals Extraction and Study Anthocyanins Plant Material

2019 ◽  
Vol 5 (4) ◽  
pp. 69-75
Author(s):  
L. Solominova ◽  
S. Onina ◽  
G. Kozlova
Keyword(s):  
Quantitative Determination ◽  
Plant Material ◽  
Water Soluble ◽  
Qualitative And Quantitative ◽  
Aronia Melanocarpa ◽  
Mahonia Aquifolium ◽  
Direct Spectrophotometry ◽  
Maximum Content ◽  
Made In

The paper presents the results of the qualitative and quantitative determination of some anthocyanins (in particular, cyanidin-3-glycoside and cyanidin-3-galactoside) in the fruits of Aronia melanocarpa (Michx.) and Mahonia aquifolium (Pursh) Nutt. The reaction of extracting water-soluble dyes on the solution medium is also presented. The method of direct spectrophotometry was used. All calculations are made in the program STATISTICA 6. It is determined that the maximum content of anthocyanins is typical for fruit chokeberry Aronia. The possibility of using natural dye from chokeberry fruits in the industry is shown.

scholarly journals METHODS FOR QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN QUERCUS ROBUR L. BUDS

2021 ◽  
Vol 9 (5) ◽  
pp. 356-366
Author(s):  
N. A. Ryabov ◽  
V. M. Ryzhov ◽  
V. A. Kurkin
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Ethyl Alcohol ◽  
Quercus Robur ◽  
Raw Materials ◽  
Uv Spectrophotometry ◽  
Total Flavonoids ◽  
Plant Raw Materials ◽  
Wide Range

Currently, the actual task of modern pharmacy is to study the chemical composition and pharmacological properties of plant objects. Within the framework of this concept, it seems interesting to study Quercus robur L. buds. One of the promising groups of biologically active compounds of Quercus robur L. buds are flavonoids. This group of substances has a wide range of a pharmacological activity, which is significant in the creation of new medicines based on medicinal plant raw materials.The aim of the article was to work out methods for quantitative determination of total flavonoids in Quercus robur l. buds.Materials and methods. The research materials were aqueous-alcoholic extracts from Quercus robur L. buds with 70% ethyl alcohol which were analyzed by differential UV spectrophotometry on spectrophotometer “SF 2000” (Russia).Results. The methods for quantitative determination of total flavonoids in Quercus robur L. buds by differential UV spectrophotometry, has been developed using a standard sample of cynaroside at the analytical wavelength of 400 nm. The optimum parameters for the extraction of total flavonoids from Quercus robur L. buds have been determined. They are: the optimum extractant is 70% ethyl alcohol; the “raw material-extractant” ratio is 1:50; the extraction time is 120 min, the degree of atomization is 2 mm.The content of total flavonoids for Quercus robur L. buds has been determined; it varies from 0.27%±0.01 to 0.44%±0.02. These results make possible to recommend the content of total flavonoids for this type of raw materials not less than 0.25% as a lower limit.Conclusion. The data obtained in the course of the experiment, makes it possible to conclude that a further study of Quercus robur L. buds is promising, and it also contributes to the implementation of medicinal plant raw materials “Quercus robur L. buds” in the State Pharmacopoeia (Russia).

Optimization of Extraction Parameters of Reverse Iontophoretic Determination of Blood Glucose in an Artificial Skin Model

2020 ◽  
Vol 16 (6) ◽  
pp. 722-737
Author(s):  
Cigdem Yengin ◽  
Emrah Kilinc ◽  
Fatma Gulay Der ◽  
Mehmet Can Sezgin ◽  
Ilayda Alcin
Keyword(s):  
Ionic Strength ◽  
Body Fluids ◽  
Extraction Time ◽  
Artificial Skin ◽  
Dialysis Membrane ◽  
Skin Model ◽  
Non Invasive ◽  
Electrode Distance ◽  
Experimental Parameters

Background: Reverse İontophoresis (RI) is one of the promising non-invasive technologies. It relies on the transition of low magnitude current through the skin and thus glucose measurement becomes possible as it is extracted from the surface during this porter current flow. Objective: This paper deals with the development and optimization of an RI determination method for glucose. CE dialysis membrane based artificial skin model was developed and the dependence of RI extraction on various experimental parameters was investigated. Method: Dependence of RI extraction performance on noble electrodes (platinum, silver, palladium, ruthenium, rhodium) was checked with CA, CV and DPV, in a wide pH and ionic strength range. Optimizations on inter-electrode distance, potential type and magnitude, extraction time, gel type, membrane MWCO, usage frequency, pretreatment, artificial body fluids were performed. Results: According to the optimized results, the inter-electrode distance was 7.0 mm and silver was the optimum noble metal. Optimum pH and ionic strength were achieved with 0.05M PBS at pH 7.4. Higher glucose yields were obtained with DPV, while CA and CV achieved almost the same levels. During CA, +0.5V achieved the highest glucose yield and higher potential even caused a decrease. Glucose levels could be monitored for 24 hours. CMC gel was the optimum collection media. Pretreated CE membrane with 12kD MWCO was the artificial skin model. Pretreatment affected the yields while its condition caused no significant difference. Except PBS solution (simulated as artificial plasma), among the various artificial simulated body fluids, intestinal juice formulation (AI) and urine formulation U2 were the optimum extraction media, respectively. Conclusion: In this study, various experimental parameters (pretereatment procedure, type and MWCO values of membranes, inter-electrode distance, electrode material, extraction medium solvents, ionic strength and pH, collection medium gel type, extraction potential type and magnitude, extraction time and etc) were optimized for the non-invasive RI determination of glucose in a CE dialysis membrane-based artificial skin model and various simulated artificial body fluids.

scholarly journals Determination of Antioxidant Parameters of Pleurotus Mushrooms Growing on Different Wood Substrates

2017 ◽  
Vol 61 (4) ◽  
pp. 53-58
Author(s):  
I. Strapáč ◽  
M. Kuruc ◽  
M. Baranová
Keyword(s):  
Oyster Mushroom ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Fruiting Bodies ◽  
Pleurotus Pulmonarius ◽  
Antioxidant Parameters ◽  
Oyster Mushrooms ◽  
Maximum Content ◽  
Β Carotene

AbstractExtracts of the fruiting bodies of the Oyster mushroom (Pleurotus ostreatus) grown on wood substrates (beech, oak, linden, walnut, poplar) and extracts of the fruiting bodies of the Oyster mushroom (Pleurotus pulmonarius) grown in nature on aspen wood were used to determine the total phenols, total flavonoids, lycopene and β-carotene. The content of individual antioxidants varies considerably depending, not only on the substrate, but also on the extracting agents. The highest content of total phenols and total flavonoids was found in methanol and water extracts of the fruiting bodies of the Oyster mushrooms grown on oak and linden substrates. The maximum content of lycopene and β-carotene was determined in acetone and n-hexane (ratio 4 : 6) extracts of the fruiting bodies of the Oyster mushroom grown on an oak block. The results obtained in this study demonstrated that the quantitative and also probably the qualitative composition of the antioxidants in the fruiting bodies of Oyster mushrooms depended considerably on the substrate composition.

Determination of Ethyl Alcohol in Saliva

1936 ◽  
Vol 34 (4) ◽  
pp. 504-505 ◽  
Author(s):  
J. C. Abels
Keyword(s):  
Ethyl Alcohol

scholarly journals Variations in Triterpenoid Deposition in Cuticular Waxes during Development and Maturation of Selected Fruits of Rosaceae Family

2020 ◽  
Vol 21 (24) ◽  
pp. 9762
Author(s):  
Soyol Dashbaldan ◽  
Cezary Pączkowski ◽  
Anna Szakiel
Keyword(s):  
Metabolic Pathways ◽  
Cuticular Waxes ◽  
Rosa Rugosa ◽  
Phenological Stages ◽  
Aronia Melanocarpa ◽  
Fruit Samples ◽  
Black Chokeberry ◽  
Rosaceae Family ◽  
Fruit Surface

The process of fruit ripening involves many chemical changes occurring not only in the mesocarp but also in the epicarp, including changes in the triterpenoid content of fruit cuticular waxes that can modify the susceptibility to pathogens and mechanical properties of the fruit surface. The aim of the study was the determination of the ripening-related changes in the triterpenoid content of fruit cuticular waxes of three plant species from the Rosaceae family, including rugosa rose (Rosa rugosa), black chokeberry (Aronia melanocarpa var. “Galicjanka”) and apple (Malus domestica var. “Antonovka”). The triterpenoid and steroid content in chloroform-soluble cuticular waxes was determined by a GC-MS/FID method at four different phenological stages. The profile of identified compounds was rather similar in selected fruit samples with triterpenoids with ursane-, oleanane- and lupane-type carbon skeletons, prevalence of ursolic acid and the composition of steroids. Increasing accumulation of triterpenoids and steroids, as well as the progressive enrichment of the composition of these compounds in cuticular wax during fruit development, was observed. The changes in triterpenoid content resulted from modifications of metabolic pathways, particularly hydroxylation and esterification, that can alter interactions with complementary functional groups of aliphatic constituents and lead to important changes in fruit surface quality.

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