METHODS FOR QUANTITATIVE DETERMINATION  OF TOTAL FLAVONOIDS IN QUERCUS ROBUR L. BUDS

Currently, the actual task of modern pharmacy is to study the chemical composition and pharmacological properties of plant objects. Within the framework of this concept, it seems interesting to study Quercus robur L. buds. One of the promising groups of biologically active compounds of Quercus robur L. buds are flavonoids. This group of substances has a wide range of a pharmacological activity, which is significant in the creation of new medicines based on medicinal plant raw materials.The aim of the article was to work out methods for quantitative determination of total flavonoids in Quercus robur l. buds.Materials and methods. The research materials were aqueous-alcoholic extracts from Quercus robur L. buds with 70% ethyl alcohol which were analyzed by differential UV spectrophotometry on spectrophotometer “SF 2000” (Russia).Results. The methods for quantitative determination of total flavonoids in Quercus robur L. buds by differential UV spectrophotometry, has been developed using a standard sample of cynaroside at the analytical wavelength of 400 nm. The optimum parameters for the extraction of total flavonoids from Quercus robur L. buds have been determined. They are: the optimum extractant is 70% ethyl alcohol; the “raw material-extractant” ratio is 1:50; the extraction time is 120 min, the degree of atomization is 2 mm.The content of total flavonoids for Quercus robur L. buds has been determined; it varies from 0.27%±0.01 to 0.44%±0.02. These results make possible to recommend the content of total flavonoids for this type of raw materials not less than 0.25% as a lower limit.Conclusion. The data obtained in the course of the experiment, makes it possible to conclude that a further study of Quercus robur L. buds is promising, and it also contributes to the implementation of medicinal plant raw materials “Quercus robur L. buds” in the State Pharmacopoeia (Russia).

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Isolation and determination of some physical and chemical properties of polysaccharide from black elderberry flowers

Herald of Tver State University. Series: Biology and Ecology ◽

10.26456/vtbio163 ◽

2020 ◽

pp. 95-101

Author(s):

Игорь Анатольевич Сычев ◽

Татьяна Викторовна Алимкина

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Raw Materials ◽

Chemical Properties ◽

Physical And Chemical Properties ◽

Plant Raw Materials ◽

Physical And Chemical ◽

And Chemical Properties

Предложенный метод количественного определения восстанавливающих моносахаридов в полисахариде позволяет проводить стандартизацию лекарственного растительного сырья цветков бузины черной. На способ стандартизации лекарственного растительного сырья цветков бузины черной по содержанию восстанавливающих моносахаридов в полисахариде оформлено рационализаторское предложение № 1373 от 29.02.2016 г. РязГМУ. The proposed method for the quantitative determination of reducing monosaccharides in a polysaccharide makes it possible to standardize medicinal plant raw materials of black elderberry flowers. On the method of standardization of medicinal plant raw materials of black elderberry flowers in terms of the content of reducing monosaccharides in the polysaccharide, a rationalization proposal No. 1373 dated February 29, 2016 RyazSMU was issued.

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Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.

Drug development & registration ◽

10.33380/2305-2066-2020-9-4-107-114 ◽

2020 ◽

Vol 9 (4) ◽

pp. 107-114

Author(s):

A. E. Sukhanov ◽

A. N. Stavrianidi ◽

E. D. Kubasova ◽

A. S. Panasyuk ◽

O. V. Buyuklinskaya

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Plant Extracts ◽

Raw Materials ◽

Biologically Active ◽

Fenugreek Seeds ◽

Plant Raw Materials ◽

Object Of Study ◽

Trigonella Foenum Graecum L

Introduction. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts.Aim. Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds.Materials and methods. The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017.Results and discussion. Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed.Conclusion. It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.

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THE DEVELOPMENT OF METHOD OF QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN THE FLOWERS OF CRATAEGUS SUBMOLLIS SARG.

chemistry of plant raw material ◽

10.14258/jcprm.2019035201 ◽

2019 ◽

pp. 137-144 ◽

Cited By ~ 1

Author(s):

Vladimir Aleksandrovich Kurkin ◽

Ol'ga Yevgen'yevna Pravdivtseva ◽

Tat'yana Vladimirovna Morozova ◽

Anna Vladimirovna Kurkina ◽

Il'nur Khyasyainovich Shaikhutdinov ◽

...

Keyword(s):

Quantitative Determination ◽

Raw Materials ◽

Specific Weight ◽

Raw Material ◽

High Yield ◽

Total Flavonoids ◽

Plant Raw Materials ◽

The Russian Federation ◽

Promising Type

The flowers and fruits of various species of the hawthorn genus (Crataegus L.) are widely used in domestic and foreign medical practice as cardiotonic preparations. In our opinion, Crataegus submolls Sarg. is a perspective species for collection of the raw materials. Crataegus submolls Sarg. is successfully cultivated in the Russian Federation and is distinguished by its rapid growth and high yield compared with wild-growing species. Moreover, the fresh fruits of Crataegus submolls Sarg. are used in nutrition. The flowers of Crataegus submollis Sarg. contain flavonoids, among of which, like in the case of Hawthorn blood-red the hyperoside is present. It was determined that the maximum of the absorption curve of the solution of Crataegus submollis Sarg. flowers with a solution of aluminum chloride is 412 nm. The method of quantitative determination by the differential spectrophotometry of the total flavonoids calculated on hyperoside in the Crataegus submollis Sarg. flowers has been developed. It was established that the appropriate extragent for this raw material is 70% ethanol. The level of total flavonoid in Crataegus submollis Sarg. flowers has been studied. It was established that separate parts of Crataegus submollis Sarg. flowers differ markedly in the content of total flavonoids and their specific weight in raw materials. It was established that the content of total flavonoids calculated on hyperoside is varied from 2.05±0.08% tо 3.00±0.12%. It was determined that the content of total flavonoids calculated on hyperoside in different parts of hawthorn flowers ranges from 0.98% (receptacle and calyx) to 4.53% (petals). In our opinion, Crataegus submollis Sarg. flowers are the promising type of medicinal plant raw materials.

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Development of approaches to standardization of black walnut bark

Aspirantskiy Vestnik Povolzhiya ◽

10.17816/2072-2354.2020.20.1.131-136 ◽

2020 ◽

Vol 20 (1-2) ◽

pp. 131-136

Author(s):

Natalya I. Zimenkina ◽

Vladimir A. Kurkin

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Raw Materials ◽

Juglans Regia ◽

Black Walnut ◽

Juglans Nigra ◽

Raw Material ◽

Plant Raw Materials ◽

Plant Raw Material

Significance. Black Walnut (Juglans nigra L.) is a species of trees of the Walnut family (Juglandaceae). This plant of the genus Walnut (Juglans L.) has not been sufficiently studied unlike other species e.g. Juglans regia L. This medicinal plant raw material is quite perspective, its preparations have antimicrobial, general tonic effect. We can use its leaves, unripe fruit, and the bark. However they are not widely used in medicine. In order to introduce the plants of the Walnut genus to the State Pharmacopoeia of the Russian Federation (RF State Pharmacopoeia), it is necessary to conduct of pharmacognostic studies, to develop product specification file to confirm the identification and quality of medicinal plant raw materials. The aim of this study is to develop a method of quantitative determination of flavonoids in the bark of the black walnut (Juglans nigra L.). Materials and methods. Material of the study was black walnut bark, stocked during the sap flow period (April) in 2018. The bark was skived up to 15 cm long and 23 cm wide. The bark was air-dried with the protection from direct sun light. The end of the drying was checked by the brittleness of the bark. Results. The methods of the quantitative determination of flavonoids in walnut bark has been developed. We used the differential spectrophotometry taking into consideration state standard sample of rutin at the analytical wavelength of 416 nm. The error of a single determination with a confidence level of 95% is 1.20%. Conclusion. We used the developed technique and analyzed a number of samples of black walnut bark. The content of total flavonoids in the plant raw material is 5.13 0.02% (as calculated on rutin). The flavonoid content should be at least 4.0%.

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DEvELopMENT of TEOHNiQuEs FoR THE DETERMiNATioN oF MoisTuRE (Loss oN DRYING) oF MEDiOiNAL PLANT RAW MATERiALS oF MoRPHoLoGiOAL group “FRUiT” by infrared thermogravimetric method

Aspirantskiy Vestnik Povolzhiya ◽

10.17816/2072-2354.2019.19.1.13-18 ◽

2019 ◽

Vol 19 (1-2) ◽

pp. 13-18

Author(s):

D. A Zhdanov ◽

V. B Braslavsky

Keyword(s):

Medicinal Plant ◽

Russian Federation ◽

Raw Materials ◽

The State ◽

Moisture Loss ◽

Anethum Graveolens ◽

Thermogravimetric Method ◽

Plant Raw Materials ◽

The Russian Federation

The article is devoted to the development of new and perspective for the State Pharmacopoeia of the Russian Federation (SP RF) techniques for determining of the moisture (loss on drying) of medicinal plant raw materials of the morphological group “Fruit” and medicinal plant preparations by the use of infrared thermogravimetric (IRTGM) method. The techniques for determination of moisture (loss on drying) IRTGM-method as exemplified by the fruit of the following medicinal plants: Silybum marianum (L.) Gaertn., Rosa cinnamomea L. and Anethum graveolens L. were developed. The comparable results for determining of the moisture (loss on drying) of the medicinal plant raw materials of investigated plants were obtained by means of two methods: IRTGM and pharmacopoeial method, which allow us to recommend the developed techniques for inclusion into the relevant monographs of the State Pharmacopoeia of the Russian Federation.

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QUANTITATIVE DETERMINATION OF XANTHONE SUM IN TERMS OF MANGIFERIN IN AERIAN ORGANS OF SPECIES OF GENUS HEDYSARUM L. BY UV SPECTROPHOTOMETRY

chemistry of plant raw material ◽

10.14258/jcprm.2020034553 ◽

2020 ◽

pp. 179-186

Author(s):

Dzhavgarat Ruslanovna Imachuyeva ◽

Fatima Kazbekovna Serebryanaya ◽

Ifrat Nazimovich Zilfikarov

Keyword(s):

Quantitative Determination ◽

Raw Materials ◽

Chemical Properties ◽

Raw Material ◽

Uv Spectrophotometry ◽

Highly Sensitive ◽

Material Procurement ◽

Physical And Chemical ◽

Study Species

Preparing of the antiviral and antibacterial medicinal plants is a promising task of native pharmacy. In this regard, it is interesting to study species of the genus penny, namely Hedysarum caucasicum M.Bieb., Hedysarum grandiflorum Pall., Hedysarum daghestanicum Rupr. ex. Boiss., the regions of distribution, chemical composition and known pharmacological properties will allow to justify directions of their rational use, as well as creation of new phytopreparations of antiviral action on the basis of medicinal raw materials. During the complex pharmacognostic studies of three species of Hedysarum L. we have developed the method for isolation and quantitative determination of the sum of xanthones in terms of mangiferin by UV spectrophotometry. The technique takes into account the basic physical and chemical properties of xanthones, is characterized by reproducibility, high accuracy, simplicity of implementation, allows to carry out both screening evaluation of various raw objects containing mangiferin derivatives, as well as standardization of the prepared medicinal plant raw material of the penny. The developed methods are tested on the aerian organs of Hedysarum L. species, collected and dried taking into account the rules and requirements of drug raw material procurement. As a result, Hedysarum caucasicum M.Bieb herb (0.624%) was found to be the highest quantitative amount of xanthone sum in terms of mangiferin. The obtained results show the prospect of further research of the aerian part of the Hedysarum caucasicum M.Bieb. as an additional source of mangiferin. The method of quantitative determination of xanthone sum in the list for mangiferin in the above-ground part of Hedysarum caucasicum M.Bieb; Based on the obtained data, it has been found that the proposed technique is highly sensitive, reproducible and usable.

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FLAVONOIDS FROM THE BUDS OF THE HORSE CHESTNUT (AESCULUS HIPPOCASTANUM L.)

chemistry of plant raw material ◽

10.14258/jcprm.2020036330 ◽

2020 ◽

pp. 123-129

Author(s):

Vladimir Aleksandrovich Kurkin ◽

Pavel Viktorovich Belov

Keyword(s):

Medicinal Plant ◽

Raw Materials ◽

Uv Spectroscopy ◽

Raw Material ◽

Aesculus Hippocastanum ◽

Horse Chestnut ◽

Plant Raw Materials ◽

Wide Range ◽

New Type ◽

First Time

The buds of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae family) is a new promising species of medicinal plant raw material. The prospect of studying the buds of this plant is due to the content of flavonoids in them, which have a wide range of pharmacological effects. Flavonoids are interesting for their capillary-strengthening action, which allows them to be used in the treatment of chronic venous insufficiency. This fact is consistent with the practice of using drugs from other organs of horse chestnut (seeds, leaves) as venotonic and angioprotective drugs. In addition, flavonoids are also characterized by antioxidant, anti-inflammatory, antimicrobial and antifungal activity. The actuality of the study of horse chestnut buds as a new type of medicinal plant material is also supported by the need to develop of the new effective and safe drugs based on the raw materials of this plant. The aim of this study was to determine the flavonoid composition of the buds of Aesulus hippocastanum. In the course of the work from the buds of horse chestnut by the method of column chromatography on silica gel L 100/160 was for the first time there were isolated and characterized using 1H-NMR, 13C-NMR, UV spectroscopy and mass spectrometry the flavonoid compounds rhamnocitrin (7-O-methylkaempferol), and accompanying 7,4′-dimethylkaempferol. The dominant and diagnostic significant flavonoid for the horse chestnut buds is rhamnocitrin. The results indicate the prospects for further study of the buds of the horse chestnut as a new type of medicinal plant raw materials.

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The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material

JOURNAL of SIBERIAN MEDICAL SCIENCES ◽

10.31549/2542-1174-2021-4-17-26 ◽

2021 ◽

pp. 17-26

Author(s):

V.V. Velichko ◽

◽

D.S. Kruglov ◽

Keyword(s):

Spectrophotometric Determination ◽

Raw Materials ◽

Biologically Active ◽

Raw Material ◽

Health Improvement ◽

Plant Materials ◽

Plant Raw Materials ◽

Wide Range ◽

Vitamin Activity

Carotenoids are of interest as precursors of vitamin A, which has a wide range of pharmacological effects — antioxidant, immunomodulatory, participating in photoreception, etc. The aim of the research was to develop a technique for the spectrophotometric determination of the A-vitamin activity of carotenoids-rich medicinal plant raw materials (MPRM). The objects of research were the traditional sources of carotenoids — sea buckthorn, carrot, pumpkin and wild rose. A method is proposed for determining the content of individual carotenoids in MPRM by optical density at wavelengths corresponding to the characteristic peaks of α-carotene, β-carotene and lycopene. It has been shown that it is preferable to use isopropyl alcohol as the most effective solvent. Taking into account the content of specific carotenoids makes it possible to determine the A-vitamin activity of the sum of carotenoids extracted from plant materials, and, accordingly, to increase the efficiency of the use of herbal preparations and biologically active additives for health improvement.

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