Preparation of zinc oxide nanoparticles using punica granatum and elettaria cardamomum fruit extract and determining its antibacterial activity against lactobacillus

To determine the antibacterial activity of Zinc oxide nanoparticles synthesised using Punica granatum and Elettaria cardamomum fruit extract against Lactobacillus. Punica granatum and Elettaria cardamomum have been known for their antimicrobial, antioxidant and anti-inflammatory activity. The phytochemicals present in these fruits have experimented for the preparation of various metal and metal oxide nanoparticles. Zinc oxide is a widely used metal oxide nanoparticle known for its good antimicrobial activity against a host of microbes. The current study was conducted to determine its effect against Lactobacillus, a bacteria known for its role in the progression of dental caries. Preparation of fruit extract mediated zinc oxide nanoparticles. Determining the characteristics of the nanoparticles using UV spectroscopy and SEM. Analysing the activity of these nanoparticles against Lactobacillus using agar well diffusion method. The zone of inhibition increased if the concentration of the fruit mediated zinc oxide nanoparticles increased. But it was incomparable to the standards. Hence, further studies need to be conducted using different concentration of Punica granatum and Elettaria cardamomum to determine the optimum fruit extract required for the preparation of the nanoparticles. The resultant nanoparticles can be used as an effective antimicrobial agent against Lactobacillus

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Synthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Lippia adoensis (Koseret) and Evaluation of Its Antibacterial Activity

Journal of Chemistry ◽

10.1155/2020/7459042 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Meron Girma Demissie ◽

Fedlu Kedir Sabir ◽

Gemechu Deressa Edossa ◽

Bedasa Abdisa Gonfa

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Antibacterial Activity ◽

Medicinal Plant ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Leaf Extract ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Lippia Adoensis

The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.

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Utility of Zinc Oxide Nanoparticles Catalytic Activity in the Electrochemical Determination of Minocycline Hydrochloride

Polymers ◽

10.3390/polym12112505 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2505

Author(s):

Amal M. Al-Mohaimeed ◽

Wedad A. Al-Onazi ◽

Maha F. El-Tohamy

Keyword(s):

Zinc Oxide ◽

Metal Oxide ◽

Zinc Oxide Nanoparticles ◽

Metal Oxide Nanoparticles ◽

Electrochemical Determination ◽

Electrochemical Sensing ◽

Oxide Nanoparticles ◽

Regression Equations ◽

Minocycline Hydrochloride

The current work described the synthesis and characterization of zinc oxide nanoparticles (ZnONPs) and their electrocatalytic activity in the determination of minocycline hydrochloride (MCL). The unique features of metal oxide nanoparticles such as zinc oxide encourage the researchers to investigate the activity of metal oxide nanoparticles as remarkable semiconductor materials active in the electrochemical sensing determination. Herein, the suggested study displayed a comparative determination of minocycline hydrochloride using two conventional and modified ZnONPs-coated wire sensors. The recorded results showed the linear behavior of the enriched ZnONPs sensor over the 1.0 × 10−10–1.0 × 10−2 mol L−1 with respect to 1.0 × 10−6–1.0 × 10−2 mol L−1 for the conventional sensor. The two sensors are working in the pH range of 3–5 with regression equations EmV = (53.2 ± 0.5) log [MCL] + 448.8 and EmV = (58.7 ± 0.2) log [MCL] + 617.76 for conventional and enriched ZnONPs, respectively. The correlation coefficients were 0.9995 and 0.9998 for the previously mentioned sensors, respectively. The validity of the suggested analytical method was evaluated according to the recommended guidelines for methodology and drug analysis. The developed sensors were also used in the quantification of MCL in commercial formulations.

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Biosynthesis of zinc oxide nanoparticles by using fruits extracts of Ananas Comosus and its antibacterial activity

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v15n2.1217 ◽

2019 ◽

Vol 15 (2) ◽

pp. 268-273 ◽

Cited By ~ 3

Author(s):

Raja Adibah Raja Ahmad ◽

Zawati Harun ◽

Mohd Hafiz Dzarfan Othman ◽

Hatijah Basri ◽

Muhamad Zaini Yunos ◽

...

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Metallic Nanoparticles ◽

Ananas Comosus ◽

Biological Synthesis ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

X Ray

Biosynthesis of metallic nanoparticles using plants, enzymes, and microorganism have been known as eco-friendly alternatives to conventional physical and chemical methods. Recently, the biological synthesis of nanoparticles has been a keen interest amongst researchers and scientist due to its simple technique, eco-friendliness, non-toxic, inexpensive and potential to perform in antibacterial activity. Thus, in this current work, the synthesis of zinc oxide (ZnO) nanoparticles using reduction agent from fruit extracts of Ananas Comosus is reported. The biosynthesized zinc oxide was characterized using Field Emission Scanning Electron Microscope (FESEM) with Energy Dispersive X-ray analysis (EDX), UV-Vis absorption spectroscopy and X-ray diffraction (XRD). The average size of the nanoparticles was found to be in the range of 30-57nm. The antibacterial activity of ZnO nanoparticles was carried out via agar diffusion method against pathogenic organisms. It is observed that the biosynthesized ZnO in the process has the efficient antibacterial activity. In conclusion, the green synthesis of zinc oxide nanoparticles using the fruit extract of Ananas Comosus is considered as a potential additive to substitute other metal oxides such as silver (Ag) and titanium dioxide (TiO2)but also provide antibacterial effect that able to enhance the nanoparticle performance.

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Green Synthesis of Zinc Oxide Nanoparticles from Pomegranate (Punica granatum) Extracts and Characterization of Their Antibacterial Activity

Molecules ◽

10.3390/molecules25194521 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4521 ◽

Cited By ~ 1

Author(s):

Ugochi Lydia Ifeanyichukwu ◽

Omolola Esther Fayemi ◽

Collins Njie Ateba

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Electron Microscope ◽

Zinc Oxide Nanoparticles ◽

Punica Granatum ◽

Antimicrobial Efficacy ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Absorption Bands ◽

Scanning Electron

This study assessed the antimicrobial efficacy of synthesized zinc oxide nanoparticles produced using aqueous extracts of pomegranate leaves and flowers designated ZnO-NPs-PL, ZnO-NPs-PF. In the study, oxides of zinc were successfully employed to fabricate nanoparticles using extracts from leaves and flowers of pomegranate (Punica granatum). The nanoparticles obtained were characterized spectroscopically. X-ray diffractive analysis (XRD) revealed the elemental components and nature of the synthesized particles. The fabricated zinc oxide nanoparticle (ZnO-NPs) showed a crystalline structure. The morphology of the nanoparticles as shown by scanning electron microscopy (SEM) was unevenly spherical and the functional groups involved in stabilization, reduction and capping were confirmed using Fourier Transform Infra-Red (FT-IR) Spectroscopy. Confirmation of the nanoparticles by UV–Vis analysis showed absorption bands of 284 and 357 nm for pomegranate leaf and flower extract, respectively, mediated ZnO-NPs. Evaluation of the antimicrobial efficacy of the fabricated nanoparticles showed that ZnO-NPs were effective against all selected pathogenic strains, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Salmonella diarizonae, Salmonella typhi, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Moraxella catarrhalis, Aeromonas hydrophila and Listeria monocytogenes, used in the analysis. The effectiveness of these nanoparticles could be linked to their sizes and shapes as obtained using a transmission electron microscope (TEM) and scanning electron microscope (SEM). Our reports revealed that increasing the concentration of the nanoparticles resulted in an increase in the antibacterial activity exerted by the nanoparticles, thus suggesting that both ZnO-NPs can effectively be used as alternative antibacterial agents. Further research is required to assess their mechanisms of action and toxicity.

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Effect of Zinc Oxide Nanoparticles on Loaded Antibiotics Against Multidrug-Resistant Acinetobacter spp.

Avicenna Journal of Clinical Microbiology and Infection ◽

10.34172/ajcmi.2021.10 ◽

2021 ◽

Vol 8 (2) ◽

pp. 51-56

Author(s):

Behnaz Shokrollahi ◽

Akram Sadat Tabatabaee Bafroee ◽

Tayebeh Saleh

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Metal Oxide Nanoparticles ◽

Antibacterial Properties ◽

Multidrug Resistant ◽

Diffusion Method ◽

Resistant Bacteria ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray

Background: Metal oxide nanoparticles (NPs) have shown promising efficacy for combating bacterial resistance due to their antibacterial properties. This research investigated the effect of zinc oxide NPs (ZnO-NPs) on the antibacterial activity of conventional antibiotics including ciprofloxacin (CIP), cefotaxime (CTX), and colistin (CST) against multidrug-resistant Acinetobacter isolates. Methods: The disc diffusion method was performed to detect the pattern of antibiotic resistance in isolates. The synthesized ZnO-NPs via the solvothermal method were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). Finally, the broth microdilution technique was conducted to demonstrate the antibacterial activity of CIP, CTX, and CST antibiotics with and without a sub-inhibitory concentration of ZnO-NPs. Results: XRD, EDS, and FESEM results confirmed the crystalline structure of ZnO-NPs, and the average size was 100±58.68 nm. All isolates were discovered to be of multidrug-resistant (MDR) type and fully susceptible to CST. The antibacterial activity of CTX and CIP was restored when combined with a sub-inhibitory level of ZnO-NPs (0.25 mg/L), and the highest activity was obtained at the concentrations of 32 µg/mL CTX and 8 µg/ mL CIP. Eventually, ZnO-NPs showed a synergistic effect on the antibacterial properties of CST against MDR Acinetobacter. Conclusions: This research indicated that the combination of ZnO-NPs with some common antibiotics can be considered as a novel strategy for reducing the spread of antibiotic-resistant bacteria.

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Facile Green Synthetic Route to the Zinc Oxide (ZnONPs) Nanoparticles: Effect on Green Peach Aphid and Antibacterial Activity

Journal of Agricultural Science ◽

10.5539/jas.v9n2p131 ◽

2017 ◽

Vol 9 (2) ◽

pp. 131 ◽

Cited By ~ 2

Author(s):

Alaa Y. Ghidan ◽

Tawfiq M. Al-Antary ◽

Nidá M. Salem ◽

Akl M. Awwad

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Green Peach Aphid ◽

Punica Granatum ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

One Step ◽

Ft Ir ◽

Average Size

In this study, zinc oxide nanoparticles (ZnONPs) were synthesized using Punica granatum peel extract in one–step reaction at room temperature. Zinc oxide nanoparticles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet visible spectroscopy (UV-vis) and scanning electron microscopy (SEM). The UV-vis absorption spectrum shows an absorption band at 278 nm due to ZnO nanoparticles. XRD characterized the final product as highly crystalline ZnO with sizes in the range 10-40 nm. The SEM results reveal a presence of network of randomly oriented ZnO nanoplatelets with an average size of 40 nm and thicknesses of about 8 nm. This study determined the effect of zinc oxide nanoparticles on green peach aphid and antibacterial activity.

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Nano ZnO and its Perspective in Anti-Cancer Activities

Mapana Journal of Sciences ◽

10.12723/mjs.41.5 ◽

2017 ◽

Vol 16 (2) ◽

pp. 41-52

Author(s):

N Shobhaa ◽

N Nandab ◽

B M Nagabhushana

Keyword(s):

Zinc Oxide ◽

Metal Oxide ◽

Zinc Oxide Nanoparticles ◽

Metal Oxide Nanoparticles ◽

Nano Particles ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Large Bandwidth ◽

Anti Cancer ◽

Potential Applications

Several methods are used to counter the deadly disease cancer. Zinc oxide nanoparticles (ZnO NPs) is one of the metal oxide nanoparticles which had been used in anti-cancer activities due to its large bandwidth and high exciting binding energy and it has potential applications like antibacterial, antifungal, anti-diabetic, anti-inflammatory, wound healing, antioxidant, optic properties and also which holds promise to treat cancer effectively. Studies have shown that Zinc metal oxide nano particles induce cytotoxicity in cancer cells. The mechanism for antitumor could work through apoptosis or the generation of reactive oxygen species or and necrosis, among other possibilities. This review is on some of the most significant antitumor results obtained with zinc oxide nanoparticles depending on their size, surface morphology, methods of preparation and also cytotoxicity result.

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A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1397 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Hams H. H. Alfattli ◽

Ghufran Zuhair Jiber ◽

Ghaidaa Gatea Abbass

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Quercus Robur ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Significant Inhibition ◽

Oxide Nanoparticles ◽

Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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