scholarly journals APPRAISEMENT OF SALINITY STRESS ON GERMINATION AND SEEDLING DEVELOPMENT OF OKRA (Abelmoschus Esculentus L.)

2020 ◽  
Vol 2 (1) ◽  
pp. 52-56
Author(s):  
Md. Morshedul Islam ◽  
Akhinur Shila ◽  
Pijush Kanti Jhan ◽  
Mehede Hassan Rubel ◽  
Kawsar Hossen ◽  
...  

Salt stress is one of the most common abiotic factors which has a trouble impression on the seed germination and establishment of crops. A laboratory based incubation experiment was executed at the department of Agriculture, Noakhali Science and Technology University during the period from January to February 2019 to investigate the salt stress effects on germination and seedling development of okra. The experiment comprises on okra variety named ‘Green Finger F1’.There were eight treatments viz. T0 (0 mM NaCl), T1 (20 mM NaCl), T2 (50 mM NaCl), T3 (100 mM NaCl), T4 (150 mM NaCl), T5 (200 mM NaCl), T6 (250 mM NaCl) and T7 (300 mM NaCl) and the experiment was designed at Completely Randomized Design (CRD) with three replications. Different parameters like germination percentage, speed of germination, seedling height, shoot elongation rate, shoot fresh and dry weight, root length, root fresh and dry weight and finally vigor index were compared among the all treatments. From the findings of this research trial, it can be extracted that the treatments T0 to T2 okra performed well as compare to the other treatments. So this study clearly indicated that the okra could be a promising crop for coastal areas and lead to improvements in agricultural production where mild saline condition present. However, it will not grow well under a high saline condition.

2019 ◽  
Vol 31 (2) ◽  
pp. 44-52
Author(s):  
Sirwa A. Qadir

Drought is one of the most critical abiotic factors especially in warm dry areas yielding limited crop. Ten wheat genotypes tested for drought tolerance at germination stage. Polyethylene Glycol-8000 used to induce -1.7 and -3.5 bars osmotic potential as compared to control treatment with three replications in factorial experiment with completely Randomized Design(CRD).The studied parameters showed a decreasing in style of response to increment of PEG concentration.The lowest  Final  Germination  Percentage  (FGP)  mean  recorded was 73.23 % under -3.5 bars as compared to control. Means of Daily Germination time (MDG), Germination Index (GI) and Coefficient of Velocity Germination (CVG) have been decreased from 4.04, 3.23 and 81.64 at control treatment to 2.46, 1.77 and 69.21 at severe drought level. But the highest CVG; 83.34 recorded by Azady under 160 g/ L PEG. In addition under -3.5 bar treatment lowest means of shoot and root length recorded; 3.34 and 1.92 cm as compared to control treatment. While, lowest shoot weight and whole seedling weight; 0.07 and 0.079 g recorded by Abu-ghreb, but lowest root dry weight 0.008 g was recorded by Adena. While, a significant increase observed in proline content in all genotypes at 160 g PEG-8000/ L medium. Ezz, Sham-6, Azady, Rabeaa and Riagary seedlings’ accumulated more proline as compared to Tammuz-2, Adena, Abu-ghreb, Abehade and Ebba-99. According to the studied parameters a dendrogram constructed. The genotypes classified into two groups. Resistant; include Ezz, Sham-6, Azady, Rabeaa and Rizgary. Sensitive; includes Tammuz-2, Adena, Abu- ghreb, Abehade and Ebba-99.


2020 ◽  
pp. 1975-1986
Author(s):  
Luma Rayane de Lima Nunes ◽  
Miller Clementino Cruz ◽  
Charles Lobo Pinheiro ◽  
Geocleber Gomes de Sousa ◽  
Alek Sandro Dutra

Salinity and water deficiency are abiotic factors which have a strong impact on agricultural activities, especially in arid and semi-arid regions. The aim of this research was to analyse the tolerance of two genotypes of forage sorghum, EA 116 and BRS Ponta Negra, to different levels of water and salt stress during germination. The conditions of water and salt stress were simulated by moistening the substrate with a solution of mannitol and of sodium chloride (NaCl) respectively, with the water potential adjusted to 0 (distilled water), -0.1, -0.2, -0.3, -0.4, -0.5 and -0.6 MPa. The variables under analysis were germination percentage, first count, germination speed index, shoot and root length, seedling dry weight, electrolyte leakage and leaf Na+/K+ ratio. The experiment was conducted in a completely randomised design, and distributed in a 2 (genotypes) × 2 (stress condition) × 7 (osmotic potential) factorial scheme, with four replications. The EA 116 and BRS Ponta Negra genotypes show greater tolerance to water stress. The EA 116 cultivar maintained germination under water stress from 0 to -0.6 MPa and saline stress from 0 to -0.4 MPa.


2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.


2019 ◽  
Vol 32 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Luma Rayane de Lima Nunes ◽  
Paloma Rayane Pinheiro ◽  
Charles Lobo Pinheiro ◽  
Kelly Andressa Peres Lima ◽  
Alek Sandro Dutra

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.


2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  


2016 ◽  
Vol 2 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Sakil Mahmud ◽  
Shayla Sharmin ◽  
Bishan Lal Das Chowdhury ◽  
Mohammad Anowar Hossain ◽  
Muhammad Javidul Haque Bhuiyan

To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 ?M MeJA and saline (12 dSm-1) + 20 ?M MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 ?M had a better effect over 20 ?M MeJA.Asian J. Med. Biol. Res. March 2016, 2(1): 74-81


2015 ◽  
Vol 67 (3) ◽  
pp. 993-1000 ◽  
Author(s):  
Lydia Shtereva ◽  
Roumiana Vassilevska-Ivanova ◽  
Tanya Karceva

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.


Author(s):  
Panayiota PAPASTYLIANOU ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS ◽  
Dimitrios BILALIS

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


2013 ◽  
Vol 35 (3) ◽  
pp. 368-373 ◽  
Author(s):  
Rodrigo de Góes Esperon Reis ◽  
Humberto Pereira da Silva ◽  
José Maria Gomes Neves ◽  
Renato Mendes Guimarães

It was aimed to verify the effect of different methodologies of osmopriming on physiological quality of gherkin seeds. Prior, it has been characterized the initial profile of the gherkin seeds. Then, the seeds were osmoprimed in gerboxes containing two blotter papers wetted with osmotic solutions in a volume equal to three times the paper dry weight and kept in BOD at 15 °C. After the priming, the seeds were dried at room temperature for 48 hours. Then, the following variables were evaluated: seeds moisture content, percentage of germination, percentage of emergence, emergence speed index and electrical conductivity. The analyses of variances were realized according to a randomized design in a factorial scheme 3 x 2 x 4: three solutes (polyetilene glycol 6000 - PEG, potassium nitrate - KNO3 and PEG + KNO3), two osmotic potentials (-0.55 and -1.10 MPa) and four times of priming (24, 48, 72 and 96 hours), followed by analyses of regression. It is concluded that the priming has no effect on germination and affects positively the vigor of the gherkin seeds lots; osmopriming with potassium nitrate is effective in improving the physiological quality of gherkin seeds lot.


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