Germination and vigour in genotypes of forage sorghum at different levels of water and salt stress

Salinity and water deficiency are abiotic factors which have a strong impact on agricultural activities, especially in arid and semi-arid regions. The aim of this research was to analyse the tolerance of two genotypes of forage sorghum, EA 116 and BRS Ponta Negra, to different levels of water and salt stress during germination. The conditions of water and salt stress were simulated by moistening the substrate with a solution of mannitol and of sodium chloride (NaCl) respectively, with the water potential adjusted to 0 (distilled water), -0.1, -0.2, -0.3, -0.4, -0.5 and -0.6 MPa. The variables under analysis were germination percentage, first count, germination speed index, shoot and root length, seedling dry weight, electrolyte leakage and leaf Na+/K+ ratio. The experiment was conducted in a completely randomised design, and distributed in a 2 (genotypes) × 2 (stress condition) × 7 (osmotic potential) factorial scheme, with four replications. The EA 116 and BRS Ponta Negra genotypes show greater tolerance to water stress. The EA 116 cultivar maintained germination under water stress from 0 to -0.6 MPa and saline stress from 0 to -0.4 MPa.

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SEED POLYMORPHISM OF RHYNCOSIA CAPITATA (ROTH) DC. ENHANCE ITS TOLERANCE TO VARYING TYPE AND INTENSITY OF SALT STRESSES

JOURNAL OF WEED SCIENCE RESEARCH ◽

10.28941/pjwsr.v26i4.852 ◽

2020 ◽

Vol 27 (2) ◽

pp. 393-401

Author(s):

Tasawer Abbas

Keyword(s):

Salt Stress ◽

Weed Management ◽

Dry Weight ◽

Threshold Level ◽

Germination Percentage ◽

Seedling Length ◽

Wide Range ◽

Management Plans ◽

The Impact ◽

Different Levels

Increasing soil salinity due to climate change complicating weed management. Rhynchosia capitata is becoming an increasing problem in summer crops, such as cotton, soybean, pearl millet and mungbean worldwide. Study was conducted to evaluate the impact of four types of salts stresses (NaCl, Na2SO4, CaCl2 and NaHCO3) at six different levels (0, 50, 100, 150, 200 and 250 mM) on R. capitata seeds of different sizes including small, medium and large. Results revealed that R. capitata can germinate over a wide range of salt stress but as the salinity level was increased to 250 mM the germination percentage and seedling growth decreased significantly. Larger seeds have more potential to germinate and grow vigorously at an increased salt concentration as compared to medium and small seeds. Salt stress caused 40-73%, 59-96% and 40-100% inhibition in seed germination, seedling length and dry weight, respectively. Among various salt stresses CaCl2 showed less inhibition of R. capitata. The higher tolerance of this weed to wide range of salt stresses is alarming factor under current and anticipated increase in salinity, as it will disturb management plans by changing critical completion period and threshold level due to more adaptability of weed under stress than crop plants.

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APPRAISEMENT OF SALINITY STRESS ON GERMINATION AND SEEDLING DEVELOPMENT OF OKRA (Abelmoschus Esculentus L.)

Tropical Agroecosystems ◽

10.26480/taec.01.2021.52.56 ◽

2020 ◽

Vol 2 (1) ◽

pp. 52-56

Author(s):

Md. Morshedul Islam ◽

Akhinur Shila ◽

Pijush Kanti Jhan ◽

Mehede Hassan Rubel ◽

Kawsar Hossen ◽

...

Keyword(s):

Salt Stress ◽

Abiotic Factors ◽

Dry Weight ◽

Shoot Elongation ◽

Germination Percentage ◽

Seedling Development ◽

Saline Condition ◽

Department Of Agriculture ◽

Stress Effects ◽

Randomized Design

Salt stress is one of the most common abiotic factors which has a trouble impression on the seed germination and establishment of crops. A laboratory based incubation experiment was executed at the department of Agriculture, Noakhali Science and Technology University during the period from January to February 2019 to investigate the salt stress effects on germination and seedling development of okra. The experiment comprises on okra variety named ‘Green Finger F1’.There were eight treatments viz. T0 (0 mM NaCl), T1 (20 mM NaCl), T2 (50 mM NaCl), T3 (100 mM NaCl), T4 (150 mM NaCl), T5 (200 mM NaCl), T6 (250 mM NaCl) and T7 (300 mM NaCl) and the experiment was designed at Completely Randomized Design (CRD) with three replications. Different parameters like germination percentage, speed of germination, seedling height, shoot elongation rate, shoot fresh and dry weight, root length, root fresh and dry weight and finally vigor index were compared among the all treatments. From the findings of this research trial, it can be extracted that the treatments T0 to T2 okra performed well as compare to the other treatments. So this study clearly indicated that the okra could be a promising crop for coastal areas and lead to improvements in agricultural production where mild saline condition present. However, it will not grow well under a high saline condition.

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Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2018-0025 ◽

2018 ◽

Vol 51 (3) ◽

pp. 51-68 ◽

Cited By ~ 1

Author(s):

M.K. Hasan ◽

M.S. Islam ◽

M.R. Islam ◽

H.N. Ismaan ◽

A. El Sabagh

Keyword(s):

Salt Stress ◽

Salinity Tolerance ◽

Seedling Growth ◽

Negative Impact ◽

Germination Rate ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Sand Culture ◽

Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

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GERMINATION AND VIGOUR IN SEEDS OF THE COWPEA IN RESPONSE TO SALT AND HEAT STRESS

Revista Caatinga ◽

10.1590/1983-21252019v32n115rc ◽

2019 ◽

Vol 32 (1) ◽

pp. 143-151 ◽

Cited By ~ 1

Author(s):

Luma Rayane de Lima Nunes ◽

Paloma Rayane Pinheiro ◽

Charles Lobo Pinheiro ◽

Kelly Andressa Peres Lima ◽

Alek Sandro Dutra

Keyword(s):

Salt Stress ◽

Heat Stress ◽

High Temperature ◽

Low Temperature ◽

Vigna Unguiculata ◽

Salt Concentration ◽

Dry Weight ◽

Germination Percentage ◽

Different Types ◽

Different Temperatures

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.

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Water-Stress Influences on Three New Promising HLB-Tolerant Citrus Rootstocks

Horticulturae ◽

10.3390/horticulturae7100336 ◽

2021 ◽

Vol 7 (10) ◽

pp. 336

Author(s):

Lidia Aparicio-Durán ◽

Frederick G. Gmitter ◽

Juan M. Arjona-López ◽

Rocío Calero-Velázquez ◽

Áurea Hervalejo ◽

...

Keyword(s):

Water Stress ◽

Water Status ◽

Abiotic Factors ◽

Dry Weight ◽

Emerging Diseases ◽

Carrizo Citrange ◽

Citrus Rootstock ◽

Chlorophyll Index ◽

Stress Influences ◽

Water Treatments

Drought and flooding conditions are increasingly common abiotic factors that affect citrus crops in both the Mediterranean Basin and Florida. Furthermore, emerging diseases, such as Huanglongbing (HLB), are a potential risk for these crops in those producing areas. This study aimed to evaluate the behavior under water-stress treatments of three new citrus rootstocks (UFR-6, B11R5T60, and 2247 x 6070-02-2) with reported tolerance of HLB, comparing them with a common commercial citrus rootstock (Carrizo citrange). Four water conditions were established: Control, Medium Water Stress (MWS), Drought, and Flooding. Chlorophyll index (SPAD), growth in height, relative growth rate, biomass (fresh and dry weight) and plant water status were evaluated. Citru rootstock response were different for each genotype; Carrizo citrange was negatively affected by all water treatments in the chlorophyll index (SPAD) and biomass production. By contrast, UFR-6 showed a positive response in SPAD and growth under MWS and Drought, B11R5T60 displayed similar behavior to Control under all water stresses, and the response of 2247 x 6070-02-2 under MWS treatment was adequate but was not under Drought or Flooding conditions. Our study describes the behavior of these promising new citrus rootstocks against water stress; B11R5T60 exhibiting the best performance. These results can be useful for the citrus industry to address water-stress problems in these crops.

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Mitigation of salt stress in rice plant at germination stage by using methyl jasmonate

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v2i1.27572 ◽

2016 ◽

Vol 2 (1) ◽

pp. 74-81 ◽

Cited By ~ 2

Author(s):

Sakil Mahmud ◽

Shayla Sharmin ◽

Bishan Lal Das Chowdhury ◽

Mohammad Anowar Hossain ◽

Muhammad Javidul Haque Bhuiyan

Keyword(s):

Salt Stress ◽

Methyl Jasmonate ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

Alpha Amylase ◽

Rice Varieties ◽

Biochemical Component ◽

Germination Stage

To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 ?M MeJA and saline (12 dSm-1) + 20 ?M MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 ?M had a better effect over 20 ?M MeJA.Asian J. Med. Biol. Res. March 2016, 2(1): 74-81

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Effect of salt stress on some sweet corn (Zea mays L. var. saccharata) genotypes

Archives of Biological Sciences ◽

10.2298/abs150121062s ◽

2015 ◽

Vol 67 (3) ◽

pp. 993-1000 ◽

Cited By ~ 3

Author(s):

Lydia Shtereva ◽

Roumiana Vassilevska-Ivanova ◽

Tanya Karceva

Keyword(s):

Zea Mays ◽

Salt Stress ◽

Zea Mays L ◽

Sweet Corn ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

F1 Hybrid ◽

Salt Stress Condition ◽

Salinity Levels

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.

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Pastoral plants for rehabilitation of degraded soil in Tunisia: the case for use of Calicotome villosa and Genista spachiana (Fabaceae)

Biologija ◽

10.6001/biologija.v63i1.3471 ◽

2017 ◽

Vol 63 (1) ◽

Author(s):

Mechergui Kaouther ◽

Jaouadi Wahbi ◽

Khouja Mohamed Larbi

Keyword(s):

Salt Stress ◽

Polyethylene Glycol ◽

Water Stress ◽

Germination Percentage ◽

Hydrophilic Polymer ◽

Degraded Soil ◽

Germination Characteristics ◽

Germination Speed ◽

Degraded Ecosystems ◽

Mean Time

Calicotome villosa and Genista spachiana are classified as vulnerable in Tunisia and they are of great interest for the rehabilitation of degraded ecosystems, food for goats and camelids, and for the use of its essential oils in phytotherapy. The aim of this study was to examine the germination characteristics of both species through analysis of the effects of the salt stress and water stress on germination. We monitored and evaluated different germination characteristics (germination percentage, mean time to germination and germination speed) in the presence of the salt and water stress. Calicotome villosa and Genista spachiana can withstand salinity of up to 15 g/l of salt (31–37% germination at 15 g/l) and also tolerate large doses of PEG6000 (30–40% germination at –1.6 MPa: Polyethylene glycol (PEG) is a hydrophilic polymer). The tolerance of the two species to salinity and water stress allows them to be a source of food for goats and camelids during drought.

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Storage Period and Different Abiotic Factors Regulate Seed Germination of Two Apocynum Species — Cash Crops in Arid Saline Regions in the Northwestern China

Frontiers in Plant Science ◽

10.3389/fpls.2021.671157 ◽

2021 ◽

Vol 12 ◽

Author(s):

Li Jiang ◽

Chaowen She ◽

Changyan Tian ◽

Mohsin Tanveer ◽

Lei Wang

Keyword(s):

Seed Germination ◽

Abiotic Stresses ◽

Arid Regions ◽

Natural Fiber ◽

Abiotic Factors ◽

Light Condition ◽

Storage Period ◽

Germination Percentage ◽

Environmental Benefits ◽

Degraded Land

On degraded land in arid regions, cultivation of Apocynum species can provide significant environmental benefits by preventing soil erosion and desertification. Furthermore, Apocynum venetum and Apocynum pictum, which are mainly distributed in salt-barren lands in the northwestern region of China, are traditionally used to produce natural fiber and herbal tea. Direct sowing of both species may encounter various abiotic stresses such as drought and salinity. However, these effects on germination remain largely unknown, especially for seeds with different storage periods. The aim of this study was to evaluate the effects of storage period, light condition, temperature regime, drought, and salinity on germination performances of both species. Germination experiment was carried out in November 2017. There were four replicates for each treatment, and each petri dish contained 25 seeds. The results indicated that prolongation of storage period significantly decreased the germination percentage and velocity, especially under abiotic stresses. Light did not affect seed germination of A. venetum and A. pictum under any conditions. Seeds had better germination performance at 10/25 and 15/30°C than those of seeds incubated at any other temperatures. With the increase of polyethylene glycol (PEG) and salinity concentrations, seed germination for both species gradually decreased, especially for seeds stored for 2 years. Low PEG (0–20%) and salinity concentration (0–200 mM) did not significantly affect germination percentage of freshly matured seeds. However, long-time storage significantly decreased drought and salinity tolerance in A. venetum and A. pictum during germination stage. For saline soils in arid and semi-arid regions, freshly matured seeds or 1-year-stored seeds of both Apocynum species are recommended to be sown by using drip-irrigation in spring.

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Emergence and vegetative development of melon in function of the soil salinity

Australian Journal of Crop Science ◽

10.21475/ajcs.19.13.03.p1551 ◽

2019 ◽

Vol 13 ((03) 2019) ◽

pp. 458-464 ◽

Cited By ~ 1

Author(s):

Daniel Teixeira Pinheiro ◽

Fabio Delazari ◽

Carlos Nick ◽

Edson Marcio Mattiello ◽

Denise Cunha Fernandes dos Santos Dias

Keyword(s):

Soil Salinity ◽

Cucumis Melo ◽

Arid Regions ◽

Seedling Emergence ◽

Saline Stress ◽

Exchangeable Sodium ◽

Vegetative Development ◽

Percentage Points ◽

Cucumis Melo L ◽

Different Levels

Soil salinity is one of the main factors limiting the development and global agricultural productivity. Melon (Cucumis melo L.) is mainly produced in arid and semi-arid regions around the world, favoring the accumulation of soluble salts in the soil. Therefore, this study aimed to evaluate the emergence and vegetative development of melon genotype under different levels of soil salinity. The assessments were performed based on the exchangeable sodium percentage (ESP) at 0, 20, 25, 30 and 35%. The emergence was affected around 30 percentage points, by comparing the control and the highest dose (35%). The emergence speed and the emergence speed index parameters were also affected in a similar way, as well as the length and dry matter of the shoot. The growth of the plants was also affected by the saline stress, being superior in the control (0%) in relation to the treatments with NaCl. The leaf area increased between weeks 4 and 7 after the planting and was slightly superior in the control in relation to the plants submitted to saline stress (20, 25, 30 and 35% of exchangeable sodium). The melon is more sensible to the saline stress in the seedling emergence than in the vegetative development over time. The development of melon seedlings is mainly affected in exchangeable sodium values superior to 20%. The analyzed hybrid is sensible to the saline stress even in 20% of exchangeable sodium.

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