scholarly journals Isolation of pathogenic microorganisms and determination of their antibiotic resistance patterns collected from different bakery products of Dhaka city

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
Author(s):  
K.K. Das ◽  
A. Sarkar ◽  
A. Hossain
Keyword(s):  
Pathogenic Bacteria ◽  
Microbial Count ◽  
Bakery Products ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
And Storage ◽  
Klebsiella Spp

Bakery products play an important part in the diet of daily life and provide vital nutrients to human. But pathogenic microorganisms may get entry to bakery items through improper handling and storage which may lead some disease to consumers. For this reason, the objective of this study was to detect pathogenic bacteria in different bakery products collected from different places of Dhaka city. In this study, commonly consumed bakery items such as sweets, biscuit, cake and bread samples were collected from different locations for the detection of pathogenic microorganism. Here, all the samples were found to be contaminated huge microbial count up to 106 CFU/g. Klebsiella spp. and Pseudomonas spp. were most predominate among all. On the other hand, Escherichia coli was totally absent in all the samples. This result indicated that bakery products prepared under unhygienic environments and which may serve as a reservoir of various pathogenic bacteria and most of them were showed resistance against common antibiotics. To ensure the health safety of consumer’s government should take necessary actions to educated food handlers.

scholarly journals Bacterial Contamination of Street Vended Food Pani Puri Available in Janakpurdham, Dhanusha

2019 ◽  
Vol 6 ◽  
pp. 70-75
Author(s):  
Nagendra Prasad Yadav ◽  
Rakesh Kumar Yadav ◽  
Satyam Prakash
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Food Samples ◽  
Microbial Count ◽  
Microbiology Laboratory ◽  
Dilution Technique ◽  
Bacillus Spp ◽  
Klebsiella Spp

Objectives: To determine the level of bacterial contamination in food (Panipuri) available in market of Janakpur, Dhanusha, Nepal. Methods: Total 120 samples of Panipuri (60 solid matters and 60 masalaa Pani) collected aseptically from the market were transported to the microbiology laboratory and processed for microbial count by serial dilution technique. Microbial load and presence of pathogen were detected in each sample. Results: Analysis of the food samples revealed that 70% of Panipuri samples had high loads of bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella spp, Pseudomonas spp, Bacillus spp.  Conclusion: This study depicted that the street vended food Panipuri of Janakpur are highly contaminated with pathogenic bacteria which can cause health risk to consumers. Thus, it is suggested that regular monitoring of the quality of street foods must be practiced to avoid any food bone illness in future.

scholarly journals Trends in antibiotic resistance patterns of methicillin resistant and methicillin sensitive Staphylococcus aureus in Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Abdullah Akhtar Ahmed ◽  
Md Abdus Salam ◽  
Md Shakhaowat Hossain ◽  
Md Babul Aktar ◽  
Nusrat Akhtar Juyee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Current Status ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Methicillin Resistant ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Background: Antimicrobial resistance in pathogens is a growing concern for health. It is a great concern around the globe about the threat of increasing antimicrobial resistance. In response to this concerns, medical experts have proposed initiatives to curtail the spread of antimicrobial resistance in pathogenic bacteria. We have taken this surveillance study in KYAMCH to contain the spread of antimicrobial resistance.Objectives: To determine the prevalence of MRSA from different clinical samples and to record its current status to commonly used anti Staphylococcus antibiotics.Methods: The clinical samples were taken to the laboratory and within four hours cultured for isolates and identification pathogens. Finally, antibiotic sensitivity testing of the isolated Staphylococcus aureus was performed. Results: More than 80% of MRSA isolates were resistant to ampicillin (98%) followed by amoxicillin, cefixime and azithromycin with 94%, 93.5% and 85% respectively and maximum sensitivity toward tobramycin with 86% followed by 85% gentamicin and 79% meropenem.Conclusion: The detected trend in antibiotic resistance patterns of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in this study is alarming. This has created a huge clinical burden in the hospital settings as well as in the community.KYAMC Journal Vol. 9, No.-1, April 2018, Page 6-10

scholarly journals Prevalence and Antibiogram Profiling of Rotten Fruits from Different Areas of Dhaka City, Bangladesh

2020 ◽  
pp. 1-9
Author(s):  
Mahmuda Akhter Akhi ◽  
Avijit Banik ◽  
Oshin Ghurnee ◽  
Nantu Chandra Das ◽  
Showmitro Nondi ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
E Coli ◽  
Daily Diet ◽  
Acinetobacter Spp ◽  
Klebsiella Spp

Fruits are highly nutritious, sources of vitamins, minerals, fibers etc. and these are part of our daily diet. However, during cultivation, harvesting, transportation, handling fruits get contaminated with pathogenic microorganisms which leads to severe problems to community. Owning to this point, in current research, bacteriological analysis was performed on Sofeda, Pineapple, Grape, Banana, Apple, Orange, Guava, papaya, Jujube and Starfruit. Total 50 samples were randomly collected from market and street vendors of Dhaka city. Higher numbers of rotten fruits were present in wholesale markets. 35 strains were isolated which included Salmonella spp., Acinetobacter spp., Klebsiella spp., Vibrio cholerae, Vibrio parahaemolyticus, Staphylocoous aureus and E. coli. They were identified using biochemical test and antibiogram for selective isolates. In case of drug resistance of isolates, majority exhibited resistance against Erythromycin, Vancomycin and Amoxycillin and showing sensitivity against Ciprofloxacin and Ceftriaxone. It was observed in the current study that 100% isolates were resistant against Erythromycin, followed by Amoxycillin 90.63% and vancomycin 86.25%, where only 35.27% isolates were resistant against Ciprofloxacin. In case of sensitivity 64.73% isolates were sensitive against Ciprofloxacin followed by Ceftriaxone 66.25%. 

scholarly journals Determination of Bacterial Factors causing Urinary Infections and its Antibiotic Resistance Patterns in Patients referred to Khorramabad Hospital, Iran

2018 ◽  
Vol 16 (4) ◽  
pp. 1-5 ◽  
Author(s):  
Fatemeh Saleh ◽  
Soheila Soleiman Nejad ◽  
Fatemeh Bahrami Chegeni ◽  
Saeede Jafari ◽  
Afshin Javanmard ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Infections ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Clinical Antibiotic Resistance Patterns Across 70 Countries

2020 ◽  
Author(s):  
Pablo Catalan ◽  
Carlos Reding ◽  
Jessica Blair ◽  
Ivana Gudelj ◽  
Jon Iredell ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Differential Resistance ◽  
Disruptive Selection ◽  
Antibiotic Resistant ◽  
Minimal Inhibitory Concentrations ◽  
Research Initiative ◽  
Clinical Resistance ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

We sought global patterns of antibiotic resistant pathogenic bacteria within the AMR Research Initiative database, Atlas. This consists of 6.5M clinical minimal inhibitory concentrations (MICs) observed in 70 countries in 633k patients between 2004 and 2017. Stratifying MICs according to pathogens (P), antibiotics (A) and countries (C), we found that the frequency of resistance was higher in Atlas than other publicly available databases. We determined global MIC distributions and, after showing they are coherent between years, we predicted MIC changes for 43 pathogens and 827 pathogen-antibiotic (PAs) pairings that exhibit significant resistance dynamics, including MIC increases and even decreases. However, many MIC distributions are multi-modal and some PA pairs exhibit sudden changes in MIC. We therefore analysed Atlas after replacing the clinical classification of pathogens into ‘susceptible’, ‘intermediate’ and ‘resistant’ with an information-optimal, cluster-based classifier to determine subpopulations with differential resistance that we denote S and R. Accordingly, S and R clusters for different PA pairs exhibit signatures of stabilising, directional and disruptive selection because their respective MICs can have different dynamics. Finally, we discuss clinical applications of a (R, dR/dt) ‘phase plane’ whereby the MIC of R is regressed against change in MIC (dR/dt), a methodology we use to detect PA pairs at risk of developing clinical resistance.

scholarly journals Microbiological surveillance of air and contact surface of pubic transports with its correlation to human infection risks

2021 ◽  
Vol 11 (1) ◽  
pp. 7-10
Author(s):  
Shah Murshid Uj Jaman Arowan ◽  
Kamal Kanta Das ◽  
Farahnaaz Feroz
Keyword(s):  
Air Pollution ◽  
Public Transport ◽  
Pathogenic Bacteria ◽  
Animal Health ◽  
Petri Dish ◽  
Human Infection ◽  
Microbial Count ◽  
Dhaka City ◽  
Negative Effects ◽  
Bacillus Spp

Over past few years, people are giving more attention to air pollution and its effects on human health. Due to the high population density in Bangladesh, air pollution is a major issue in metropolitan areas, especially in Dhaka city. Present study aims to isolate air-borne microbes from the inside environment of public transport and different types of microbes that are frequently encountered by commuters when they touch the interior surfaces of vehicles used for public transport in Dhaka City. Atmospheric load of microorganisms was measured with petri dish which was kept open for 30 minutes in the sampling locations and swabs were taken from interior surfaces of vehicles. Air samples collected from both bus and railway stations showed a high atmospheric microbial count (total viable bacteria and total fungal count were 140 to 776 CFU/plate/30 min and 27 to 168 CFU/plate/30 min, respectively). A similar outcome was found after analysis of swab samples of the contact surfaces of the vehicles as well. A total of four bacterial pathogens were identified from the interior surfaces of the vehicle including Escherichia coli, Bacillus spp., Pseudomonas spp. and Vibrio spp. which are known to be associated with gastrointestinal tract infection. To manage and control the environmental health risks caused by air pathogens, an authorized governmental agency should do continuous monitoring of air quality to reduce the negative effects and impacts of air pathogens on human and animal health. This research has shown a great concern to health practitioners in developing countries because these are pathogens that are mostly resistant to the commonly available antibiotics used in the treatment of infection associated with these pathogens. Most importantly, we need to raise awareness among the public in order to reduce the load and spread of pathogenic bacteria in the environment. Stamford Journal of Microbiology, Vol.11 (1) 2021: 7-10

scholarly journals Identification and Antimicrobial Resistance ofEnterococcusSpp. Isolated from the River and Coastal Waters in Northern Iran

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Majid Alipour ◽  
Reza Hajiesmaili ◽  
Maryam Talebjannat ◽  
Yousef Yahyapour
Keyword(s):  
Coastal Waters ◽  
Pathogenic Bacteria ◽  
Intestinal Tract ◽  
Species Level ◽  
Biochemical Tests ◽  
Northern Iran ◽  
Resistant Genes ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
Prevalent Species

As fecal streptococci commonly inhabit the intestinal tract of humans and warm blooded animals, and daily detection of all pathogenic bacteria in coastal water is not practical, thus these bacteria are used to detect the fecal contamination of water. The present study examined the presence and the antibiotic resistance patterns ofEnterococcusspp. isolated from the Babolrud River in Babol and coastal waters in Babolsar. Seventy samples of water were collected in various regions of the Babolrud and coastal waters. Isolated bacteria were identified to the species level using standard biochemical tests and PCR technique. In total, 70Enterococcusspp. were isolated from the Babolrud River and coastal waters of Babolsar.Enterococcus faecalis(68.6%) andEnterococcus faecium(20%) were the most prevalent species. Resistance to chloramphenicol, ciprofloxacin, and tetracyclin was prevalent. The presence of resistantEnterococcusspp. in coastal waters may transmit resistant genes to other bacteria; therefore, swimming in such environments is not suitable.

scholarly journals Presence of microorganisms in commonly used baby cosmetics, available in Dhaka City

2020 ◽  
Vol 9 (1) ◽  
pp. 9-11
Author(s):  
Farahnaaz Feroz ◽  
Kamal Kanta Das
Keyword(s):  
Production Process ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Storage Conditions ◽  
Dhaka City ◽  
Acceptable Limit ◽  
Fungal Load ◽  
Viable Bacteria ◽  
And Storage

Cosmetics especially baby products should be safe as children are immunocompromised which means their immune system is not developed enough to combat infections caused by the presence of any pathogenic bacteria. Different factors like chemical composition of cosmetics, handling and storage conditions can influence the chance of contamination of cosmetic products. This study aimed to evaluate the microbiological quality of different commercially available baby cosmetics such as body lotion, body wash or soap, baby shampoo, baby oil of different popular brands sold in Dhaka city. In the current investigation four different brands of cosmetics were subjected to bacteriological and mycological screening. Total viable bacteria and fungal load in all the samples of Brand 1 was in average of 105 cfu/g which exceeded the acceptable limit. The samples were found to contain pathogenic bacteria as well, especially Klebsiella spp. The findings indicate that the quality Brand 1 products were not satifactory and might cause harm to children and other consumers. Microbial load of other brands fell within the acceptable limit which indicate their good quality. The incidence of microorganisms in Brand 1 might be due to contamination during the production process or storage under unhygienic condition. Therefore in order to avoid infections in children due to microbial contamination of cosmetics items it is essential to take precautions during production process and storage in appropriate conditions and avoiding use of cosmetics products on damaged skin. Stamford Journal of Microbiology, Vol.9(1) 2019: 9-11

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