scholarly journals Effect of pH extraction buffer on antioxidant enzymes activities in water lily’s leaves and petioles

Food Research ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 34-44
Author(s):  
Z. Mohd Zin ◽  
N.H. Yahaya ◽  
N. Bashah ◽  
K. Ibrahim ◽  
N.D. Rusli ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Ornamental Plants ◽  
Value Added ◽  
Urban Ecosystem ◽  
Scanning Calorimetry ◽  
Water Lily ◽  
Enzymes Activities ◽  
Extraction Buffer ◽  
Antioxidant Enzymes Activities ◽  
Ph Range

Water lily (Nymphaea antares) is one of the most valuable aquatic ornamental plants which has bright potential in the floriculture industry. It may be useful as an urban ecosystem and as a source of medicinal compounds. Due to its potential to become a new value-added product in the food industry, water lily (N. antares) was investigated in this study. Therefore, the goal of this study was to determine the nutritional content and antioxidant activity in water lily leaves and petioles with different pH extraction buffers. Water lily extract was obtained using three different pH extraction buffers, Tris buffer at pH 6.8, pH 7.1 and pH 7.8. The heat capacity of the extract was analysed using differential scanning calorimetry (DSC) and different functional groups were identified using Fourier transform infrared spectroscopy (FTIR). Thermal denaturation of the leaves sample was detected at 81.84°C. The antioxidant enzymes activities including catalase (CAT), peroxidase (POX), polyphenol oxidase (PPO), and superoxide dismutase (SOD) were determined in the leaves and petioles of water lily. In leaves, PPO activity was found to be higher in samples with pH 7.1 of the extraction buffer while there were no significant differences for activities of CAT, POX and SOD in all samples. In petioles, PPO and POX activity were found to be higher in samples with pH 7.8 and 7.1 of extraction buffer, respectively. Thus, the study found that a pH range of 7 to 9 extraction buffers did not greatly affect most of the analysis performed.

EFFECTS OF COPPER ON ANTIOXIDANT ENZYMES ACTIVITIES AND METALLOTHIONEIN CONCENTRATION OF BELLAMYA PURIFICATA

2009 ◽  
Vol 33 (4) ◽  
pp. 717-725 ◽  
Author(s):  
Qing-Shun ZHANG ◽  
Jian-Jun HOU ◽  
Xiang-Jiang LIU ◽  
Jie-Xuan LUO ◽  
Bang-Xi XIONG
Keyword(s):  
Antioxidant Enzymes ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

scholarly journals Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

2013 ◽  
Vol 19 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Jyoti R. Rout ◽  
Shidharth S. Ram ◽  
Ritarani Das ◽  
Anindita Chakraborty ◽  
Mathummal Sudarshan ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Withania Somnifera ◽  
Protein Profile ◽  
Copper Stress ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

scholarly journals Physiological Effect of Kinetin on the Photosynthetic Apparatus and Antioxidant Enzymes Activities During Production of Anthurium

2018 ◽  
Vol 4 (5) ◽  
pp. 182-192 ◽  
Author(s):  
Fabiana B. de MOURA ◽  
Marcos R. da S. VIEIRA ◽  
Adriano do N. SIMÕES ◽  
Sérgio L. FERREIRA-SILVA ◽  
Carlos A.V. de SOUZA ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Photosynthetic Apparatus ◽  
Physiological Effect ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

scholarly journals Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 157 ◽  
Author(s):  
Shahid Rajput ◽  
Cong Zhang ◽  
Yue Feng ◽  
Xiao Wei ◽  
Mahmoud Khalil ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Basal Diet ◽  
Mitochondrial Pathway ◽  
Bursa Of Fabricius ◽  
Enzymes Activities ◽  
Study Results ◽  
Mrna And Protein Expression ◽  
Antioxidant Enzymes Activities

Aflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopathology, oxidative stress, and apoptosis via the mitochondrial pathway in the bursa of Fabricius (BF) of broilers. One hundred forty-four one-day old Cobb chicks were randomly assigned into four treatment groups of six replicates (6 birds each replicate) for 28 days. Groups were fed on the following four diets; (1) Basal diet without addition of PCs or AFB1 (Control); (2) basal diet supplemented with 1 mg/kg AFB1 from contaminated corn (AFB1); (3) basal diet supplemented with 250 mg/kg PCs (PCs); and (4) basal diet supplemented with 1 mg/kg AFB1 + 250 mg/kg PCs (AFB1+ PCs). The present study results showed that antioxidant enzymes activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) in AFB1 treated group were (p < 0.05) decreased, whereas malondialdehyde (MDA) contents were significantly increased in comparison with the control group. Furthermore, we found that dietary PCs treatment ameliorated AFB1-induced oxidative stress in the BF through inhibiting the accumulation of MDA content and enhancing the antioxidant enzymes activities (T-SOD, CAT, GSH-Px, and GST). Similarly, PCs markedly enhanced messenger RNA (mRNA) expression of antioxidant genes (SOD, CAT, GPx1, and GST) in comparison with AFB1 group. Moreover, histological results showed that PCs alleviated AFB1-induced apoptotic cells in the BF of broilers. In addition, both mRNA and protein expression results manifested that mitochondrial-apoptosis-associated genes (Bax, caspase-9, caspase-3, and p53 and cytochrome c) showed up-regulation, while (Bcl-2) showed down-regulation in AFB1 fed group. The supplementation of PCs to AFB1 diet significantly reversed the mRNA and protein expression of these apoptosis-associated genes, as compared to the AFB1 group. Our results demonstrated that PCs ameliorated AFB1-induced oxidative stress by modulating the antioxidant defense system and apoptosis in the BF through mitochondrial pathway in broilers.

The protective effect of olive cake treatment on oxidant/antioxidant biomarkers, on serum, red blood cells and liver, in streptozotocin-induced diabetic rats fed cholesterol-enriched diet

2019 ◽  
Vol 50 (4) ◽  
pp. 785-798
Author(s):  
Yahiaoui Zidan ◽  
Sherazede Bouderbala ◽  
Cherrad Hayet ◽  
Bouchenak Malika
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Diabetic Rats ◽  
Male Rats ◽  
Olive Cake ◽  
Content Type ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs) and liver, in streptozotocin (STZ)-induced-diabetic rat fed cholesterol-enriched diet. Design/methodology/approach Hypercholesterolemic male rats were rendered diabetic (HC-D) by a single intraperitoneal injection dose of STZ (35 mg/kg BW). HC-D rats were divided into two groups fed for 28d a diet supplemented with OC at 7.5 percent (HC-D-OC) or not (HC-D). A control group (C) was submitted to standard diet containing 20 per cent casein for the same experimental period. Findings RBCs, serum and liver thiobarbituric acid reactive substances (TBARS) contents were significantly increased in HC-D, compared to C group (p = 0.04, p = 0.02 and 0.03). These values were significantly decreased (48 per cent and 64 per cent; p = 0.02 and p = 0.0007) in serum and liver of HC-D-OC vs HC-D group. In RBCs, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities were, respectively, 1.5, 2- and 1.7-fold higher (p = 0.03, p = 0.008 and p = 0.03) in HC-D group compared to HC group. In serum and liver, SOD, CAT and GST activities were, respectively, 1.3-, 2.6- and 1.6-fold increased (p = 0.03, p = 0.007 and p = 0.02). In HC-D-OC compared to HC-D group, RBCs glutathione peroxidase (GSH-Px), CAT and GST activities were, respectively, 2.1-, 3.3- and 2.1-fold higher (p = 0.04, p = 0.0009 and p = 0.03). In serum, SOD and CAT activities were, respectively, 1.5- and 1.9-fold increased (p = 0.02, p = 0.02). In liver, SOD, GSH-PX, CAT and GST activities were significantly increased (p = 0.005, p = 0.03, p = 0.02 and p = 0.04). Originality/value In diabetic rats-fed cholesterol-enriched diet, OC was able to reduce oxidative stress by decreasing lipid peroxidation and increasing antioxidant enzymes activities in serum, RBCs and liver.

scholarly journals Impacts of drought stress on growth, protein, proline, pigment content and antioxidant enzyme activities in rice (Oryza sativa L. var. BRRI dhan-24)

2020 ◽  
Vol 29 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Shamima Nasrin ◽  
Shukanta Saha ◽  
Hasna Hena Begum ◽  
Rifat Samad
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Oryza Sativa L ◽  
Proline Accumulation ◽  
The Other ◽  
Antioxidant Enzyme Activities ◽  
Total Chlorophyll ◽  
Enzymes Activities ◽  
Pigment Contents ◽  
Antioxidant Enzymes Activities

One of the major problems associated with rice cultivation and production is the deficiency of water resources. In here the effect of drought stress on growth, protein-, proline-, pigment contents and antioxidant enzymes activities in rice var. BRRI Dhan-24 was determined and analyzed under drought and wellwatered condition. Drought stress caused the decrease of growth and pigment contents - chlorophyll-a, b, a/b, total chlorophyll and carotenoids content of leaves of rice plants. On the other hand, the accumulation of protein, proline contents and antioxidant enzymes activities was increased under drought stress. It may be suggested that antioxidant enzymes activities and proline accumulation were associated with the growth of the plant and consequently with the mechanisms of drought tolerance in rice. Dhaka Univ. J. Biol. Sci. 29(1): 117-123, 2020 (January)

scholarly journals Fresh Bitter Melon Fruit (Momordica charantia) Attenuated Oxidative Stress, Fibrosis and Renal Injury in Carbon Tetrachloride Treated Rats

2018 ◽  
Vol 16 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Md Abu Taher Sagor ◽  
Hasan Mahmud Reza ◽  
Nabila Tabassum ◽  
Md Moshfequr Rahman ◽  
Md Ashraful Alam
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Carbon Tetrachloride ◽  
Renal Disease ◽  
End Stage Renal Disease ◽  
Immune Cell ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities ◽  
Stage Renal Disease ◽  
End Stage

Chemical or drug-induced kidney damage is increasing every year and the end-stage renal disease is becoming a burden for health care system of many countries. Oxidative stress may be a crucial pathway for the development of end-stage renal disease. Thus, natural antioxidant or plant-based therapy would be a better alternative to protect renal function against chemical-induced renal damage. To determine these aspects we evaluated renoprotective effects of M. charantia in carbon tetrachloride administered rats. A 10% w/w mixture of fresh fruits of M. charantia was given with the chow food every day to CCl4 treated rats. After fourteen days, all animals were sacrificed and the kidneys were examined to observe the possible protective effects of M. charantia against CCl4 induced toxicity. The CCl4 treated rats showed increased oxidative stress parameters and decreased antioxidant enzymes activities. Supplementation of 10% w/w M. charantia fruits in CCl4 administered rats prevented the oxidative stress and restored the antioxidant enzymes activities. M. charantia fruits supplementation also prevented the rise of uric acid and creatinine concentration in plasma of CCl4 treated rats. Furthermore, histological studies showed that supplementation of 10% w/w M. charantia fruits prevented the collagen deposition, immune cell migration and iron deposition in kidney sections of CCl4 treated rats. The results of this study revealed that the fruits of M. charantia may protect oxidative stress-mediated damage in kidneys due to CCl4 administration, which is mediated probably via the restoration of anti-oxidant enzyme functions.Dhaka Univ. J. Pharm. Sci. 16(2): 205-214, 2017 (December)

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