scholarly journals Morphological Peculiarities of Trophic Ulcers of Venous Etiology after Cord Blood Stem Cell Transplantation

2021 ◽  
Vol 6 (1) ◽  
pp. 37-45
Author(s):  
Y. V. Olinik ◽  
◽  
D. B. Dombrovskiy ◽  
I. S. Davydenko
Keyword(s):  
Cord Blood ◽  
Blood Vessels ◽  
Cell Transplantation ◽  
Granulation Tissue ◽  
Specific Volume ◽  
Main Group ◽  
Control Group ◽  
Fibrinoid Necrosis ◽  
Venous Ulcers ◽  
Willebrand Factor

The aim. The research deals with analysis of the degree of regenerative processes activity in patients with trophic venous ulcers of the lower extremities that do not heal for a long time on the background of cell transplantation of cord blood stem cells and without. The study involved 32 patients with long-term non-healing trophic venous ulcers of venous etiology. Results and discussion. The study results showed that the healing process of the main group patients began in the first days after transplantation to reduce pericellular edema and inflammatory hyperemia of the soft tissues around the ulcer. When examining the histological features of the central skin ulcers of patients in the control group on the fifth day after treatment, we found out that the bottom surface of the ulcers was covered with homogeneous masses of the type of fibrinoid necrosis. Ulcers had a variable depth, in some places reached the fiber, with sweat and sebaceous glands, hair follicles in the course of defects were completely destroyed, in these places there were hemorrhages and young granulation tissue. In patients of the main group, the surface of the bottom of the ulcer was covered with homogeneous masses of fibrinoid necrosis, but the masses of fibrinoid necrosis were visually expressed three times less than in the control group, but the most important was that they always alternated with "veins" consisting of cells type of lymphoid. Immunohistochemical analysis for Willebrand factor in patients of the main group revealed unevenly scattered small compact groups of positively stained cells, which should be evaluated as foci of neoplasms of blood vessels more evenly distributed among the blood vessels of granulation tissue, in contrast to the control group. On the 14th day of the clinical study at the bottom of the ulcer in the main group there were morphological signs of better maturation of granulation tissue, as seen by more uniform and intensive processes of collagen fiber formation (increase in specific volume of collagen fibers) and blood vessels (decrease in specific volume of blood vessels) and the maturation of lymphoid (polypotent) cells into fibroblasts with more complete production of vimentin in them and endothelial cells with more complete production of Willebrand factor in them. It should be noted that there was a more complete resorption of fibrinoid necrosis masses in the main observation group compared to the control group of patients, which should also contribute to faster and more complete healing of the ulcer

scholarly journals Features of laboratory markers in pregnant women with antiphospholipid syndrome and retrochorial hematoma

2021 ◽  
pp. 12-19
Author(s):  
Oleh Tomniuk
Keyword(s):  
Platelet Aggregation ◽  
T Lymphocytes ◽  
Pregnant Women ◽  
Nk Cells ◽  
Antiphospholipid Syndrome ◽  
Annexin V ◽  
Main Group ◽  
Control Group ◽  
Antiphospholipid Antibody ◽  
Willebrand Factor

The aim. Study of hemostasis, antiphospholipid antibody levels and immunological parameters in pregnant women with antiphospholipid syndrome (APS), in particular with retrochorial hematoma (RCH). Materials and methods. 90 women were selected and divided into two groups: the control group – 30 pregnant women with a normal pregnancy (without APS) and the main group – 60 pregnant women with APS. Women in the main group were diagnosed with APS before pregnancy. In turn, the main group was divided into two subgroups: 1 subgroup – 41 pregnant women without RCH and 2 subgroup – 19 pregnant women with RCH. The main indicators of hemostasis were determined in all pregnant women, namely: the degree and rate of platelet aggregation, Willebrand factor, D-dimers. In addition, the level of antiphospholipid antibodies (APLA), antibodies to β2-glycoprotein, to annexin V, to prothrombin was examined, and the level of annexin V was also determined. The absolute and relative content of Treg, CD3+, T-lymphocytes, CD4+ (T-helpers), CD8+ (cytotoxic T-lymphocytes), CD19+ (B-lymphocytes), CD16+CD56+ (NK cells), CD16+CD56+CD107a+ (activated NK cells). Results. The obtained results showed that in pregnant women with APS compared to pregnant women without APS there are statistically significantly higher values of the degree and rate of platelet aggregation (90.6±6.3% and 106.3±6.7% vs. 65.3±5.3 % and 73.4±5.6%, respectively). There were also higher values ​​of Willebrand factor and D-dimers (2.5±0.3 IU/ml and 378.1±34.3 ng/ml against 1.7±0.2 IU/ml and 268.1±27, 3 ng/ml, respectively). APLAs were significantly higher in pregnant women with APS compared with pregnant women in the control group, namely: 16.1±1.5 vs. 3.8±0.4 U/ml. With regard to antibodies to β2-glycoprotein, to annexin V, to prothrombin and to the level of annexin V, their values were also statistically significantly higher in the group of pregnant women with APS. In addition, the results of the study showed that pregnant women with APS showed changes in subpopulations of immunocompetent cells. However, examining the difference in hemostasis, antibody content, and level of lymphocyte subpopulations between pregnant women with APS with and without RCH, it was found that their shifts in pregnant women with RCH were more pronounced than in women without RCH. Conclusions. Pregnant women with APS are characterized by significantly more significant changes in hemostasis, manifested by activation of intravascular thrombosis. In addition, such pregnant women had a significantly higher concentration of autoantibodies. There are also changes in the immune system, in particular, a decrease in Treg-cells, which have the ability to reduce the specific proliferation and effector functions of lymphocytes, thereby participating in the pathogenesis of APS.

scholarly journals SPECIALTIES OF HYSTOMORPHOMETRICAL CHANGES IN PLACENTA OF WOMEN WITH EARLY AND LATE PREECLAMPSIA

2020 ◽  
Vol 73 (1) ◽  
pp. 151-155 ◽  
Author(s):  
Dmitro G. Konkov ◽  
Alina O. Piskun ◽  
Oksana A. Taran ◽  
Galyna V. Kostur
Keyword(s):  
Specific Gravity ◽  
Blood Vessels ◽  
Structural Transformation ◽  
Normal Pregnancy ◽  
Blood Stream ◽  
Main Group ◽  
Control Group ◽  
Early Form ◽  
Perinatal Pathology ◽  
Placental Blood

The aim: To find out typical pathomorphological differences in placenta of women with early and late preeclampsia. Materials and methods: Investigation includes 40 placentas from deliveries in women with preeclampsia (main group) and 40 placentas from physiological delivery in somatically healthy women, who had no complications during pregnancy (control group). Placentas in the main group were devided into two sub-groups (20 in each) – with early and late preeclampsia. Specialties of the blood vessels in normal pregnancy were investigated, and their structural transformation with the developement of preeclampsia, according to the appearence of perinatal pathology. Morphometrical data of the blood stream was investigated with the help of eyepiece and program Image Tools 3,6. Results: Significant decrease of weight (p<0,05), square and volume of placenta was common to early preeclampsia, comparing to the same characteristics in late Preeclampsia (PE). Specific gravity of villi without vessels, hardened blood vessels, hardened villi and fibrinoid altered vessels was increased statistically significantly (p<0,05) in placenta of women with early PE, comparing to women with late PE. The number of effective blood vessels crossings was determined mostly in late PE, comparing to the early form (p<0,05). Found out significant defferences (p<0,05) in changes of hystovasoarchitecture of placenta in early preeclampsia, according to the number of immature villi and villi with no signs of compensatory angiomatosis. Conclusions: Increased number of hypoplasia of placenta, breach of effective placental blood stream and significant decrease of compensatory and adaptive changes in placenta are more common to early PE, comparing to late PE.

scholarly journals Morphological Characteristics of Lungs with Experimental Peritonitis on the Background of Diabetes Mellitus

2020 ◽  
Vol 11 (1) ◽  
pp. 7511-7518
Keyword(s):  
Diabetes Mellitus ◽  
Blood Vessels ◽  
Histological Examination ◽  
Lung Tissue ◽  
Main Group ◽  
Control Group ◽  
Perivascular Edema ◽  
White Rats ◽  
Experimental Peritonitis ◽  
Intact Rats

Acute generalized peritonitis (AGP) and diabetes mellitus (DB) remains an urgent problem in modern medicine, as rapid disability and high mortality have been leading to cardiovascular disease and oncology pathology in recent years. The purpose is to study and evaluate the morphological changes in the lungs in animals with experimental AGP against the background of DM. Іn the experiment, 32 white rats were used. The main group consisted of 24 animals with simulated AGP against the background of CD; control group – 8 intact animals. The experimental CD was reproduced by intraperitoneal administration of streptozotocin at a dose of 60 mg/kg. On the 14th day after administration of streptozotocin, animals of the main group were injected with 10 % filtered stool suspension into the abdominal cavity of the study rats at a dose of 0.5 ml per 100 g of body weight. Removal of material for histological examination was carried out on days 1, 3, and 7. For the morphological study of the lungs of rats under experimental peritonitis, the method of light microscopy was used. Micropreparations were stained with hematoxylin and eosin, according to Hart, by the Van Gizon method. Histological examination of the lung tissue of animals with simulated AGP against the background of CD compared with intact rats on day 1 of the experiment showed a tendency for vasodilation. For 3 days, the lungs of the organs were observed in the lungs of animals; the wall was thickened in the vessels of large and medium caliber, the phenomena of mucoid edema and perivascular edema with small cell infiltration were noted. At day 7, significant changes in blood vessels and bronchi in all components were detected in the lungs of animals; Perivascular edema and inflammatory infiltration along the interval septa were observed in animals with simulated AGP against the background of CD in comparison with intact rats for 1 day of the experiment in lung tissue. The wall of small bronchi was thickened by leukocyte infiltration. For 3 days, the lungs of the animals were observed full blood vessels of the pulmonary components, the phenomena of mucoid edema were noted. For 7 days in the lungs of animals found the expansion of the lumen of the arteries and veins from their full blood, thickening of the outer shell of the vessel wall.

scholarly journals Fetal Sex and Fetal Environment Interact to Alter Diameter, Myogenic Tone, and Contractile Response to Thromboxane Analog in Rat Umbilical Cord Vessels

2021 ◽  
Vol 12 ◽  
Author(s):  
Benoit Sicotte ◽  
Michèle Brochu
Keyword(s):  
Cord Blood ◽  
Blood Vessels ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Contractile Response ◽  
Maximal Response ◽  
Myogenic Tone ◽  
Control Group ◽  
Control Groups ◽  
Umbilical Arteries

Fetal growth needs adequate blood perfusion from both sides of the placenta, on the maternal side through the uterine vessels and on the fetal side through the umbilical cord. In a model of intrauterine growth restriction (IUGR) induced by reduced blood volume expansion, uterine artery remodeling was blunted. The aim of this study is to determine if IUGR and fetus sex alter the functional and mechanical parameters of umbilical cord blood vessels. Pregnant rats were given a low sodium (IUGR) or a control diet for the last 7 days of pregnancy. Umbilical arteries and veins from term (22 day) fetal rats were isolated and set-up in wire myographs. Myogenic tone, diameter, length tension curve and contractile response to thromboxane analog U46619 and serotonin (5-HT) were measured. In arteries from IUGR fetuses, myogenic tone was increased in both sexes while diameter was significantly greater only in male fetuses. In umbilical arteries collected from the control group, the maximal contraction to U46619 was lower in females than males. Compared to the control groups, the maximal response decreased in IUGR male arteries and increased in female ones, thus abolishing the sexual dimorphism observed in the control groups. Reduced contractile response to U46619 was observed in the IUGR vein of both sexes. No difference between groups was observed in response to 5HT in arteries. In conclusion, the change in parameters of the umbilical cord blood vessels in response to a mild insult seems to show adaptation that favors better exchange of deoxygenated and wasted blood from the fetus to the placenta with increased myogenic tone.

Linezolid Use in Hematopoietic Stem Cell Transplantation: A Cautionary Tale

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4534-4534
Author(s):  
Alison M Gulbis ◽  
Musa Yilmaz ◽  
Dawen Sui ◽  
Roland L Bassett ◽  
Zandra R Rivera ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cord Blood ◽  
Cell Transplantation ◽  
Cumulative Incidence ◽  
Control Group ◽  
Cell Transplant ◽  
Gram Positive ◽  
Hematopoietic Stem ◽  
Cell Source

Abstract Abstract 4534 Background: Linezolid is an oxazolidinone antimicrobial often used to treat resistant gram-positive bacteria. Linezolid has been associated with mild, reversible, time-dependent myelosuppression, including thrombocytopenia, anemia, leukopenia, and pancytopenia. The hematologic effects most commonly reported are thrombocytopenia and anemia. In general, these effects have occurred with treatment durations of ≥ 14 days. Patients with underlying hematologic abnormalities may be more at risk for the development of linezolid-induced myelosuppression, but this is controversial. We hypothesized that use of linezolid before engraftment may delay hematopoietic recovery following stem cell transplant (SCT), and performed a matched controlled analysis to investigate this hypothesis. Methods: With approval from our institutional review board, we retrospectively evaluated 24 patients who received linezolid and compared them to 60 controls who did not receive linezolid from 1/1/1997 to 1/1/2010. Our SCT database was utilized to find matched controls and matching was based on the following: diagnosis; transplant type (autologous, matched sibling, matched unrelated, cord blood); cell source (peripheral blood, bone marrow, cord blood); transplant conditioning regimen; and age within 10 years. Patients then underwent further screening for study enrollment. Patients in the linezolid group were included if linezolid was administered at any time from the stem cell infusion (day 0) through engraftment of white cells but for a duration of at least 72 consecutive hours. Patients 1 year of age or older were included. The data were analyzed for the effects of linezolid on time to neutrophil (first of 3 consecutive days in which ANC > 500) and platelet engraftment (first of 7 consecutive days in which platelet count was> 20,000 without transfusion), and the cumulative incidence of engraftment of both neutrophil and platelets within the first 100 days post-transplant. We also studied the subgroup of controls that received vancomycin to treat gram-positive infections. Results: The linezolid and control groups were similar with respect to age (median 44 vs. 41 years), gender (50% vs. 58% male and 50% vs. 42% female) disease type (29% vs. 25% had AML or MDS), cell source (67% vs. 63% apheresis product), transplant type (67% vs. 63% allogeneic), ablative vs. non-ablative conditioning (79% vs. 83% myeloablative), and cell dose (median CD34 dose 4.52 × 106/kg vs. 4.34 × 106/kg). Table 1 shows engraftment data. The median time to engraftment (ANC plus platelets) for linezolid group vs. control group was 50 days (11 patients censored) vs. 15.5 days (10 patients censored). With regard to engraftment failures, 16% of patients in the linezolid group vs. 7% in the control group failed to reach ANC >500. Forty-six percent of patients in the linezolid group vs. 13% in the control group failed to achieve platelet engraftment >20,000. Figure 1 shows overall survival. Day 100 survival rates: 58% for the linezolid group vs. 92% for controls. The cumulative incidence of engraftment of both neutrophils and platelets (using death without engraftment as the competing risk) was significantly lower in the linezolid group (54%) compared to the control group (83%) (p=0.005) Conclusions: Linezolid does not significantly affect time to neutrophil engraftment, but it does appear to significantly prolong time to platelet engraftment when compared to patients who did not receive linezolid. Linezolid should be used cautiously early after stem cell transplantation. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Clinical and functional aspects of cord blood cell transplantation in patients with distal lesion of lower limb arteries

2020 ◽  
Vol 9 (2) ◽  
pp. 952-955
Keyword(s):  
Blood Cell ◽  
Cord Blood ◽  
Cell Transplantation ◽  
Laser Doppler Flowmetry ◽  
Conservative Therapy ◽  
Well Being ◽  
Laser Doppler ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Cord Blood Cell

To carry out a comparative analysis of the clinical condition of patients with distal lesions of the arterial bed using standard conservative therapy with cord blood cell transplantation and without it. The experimental group included patients with manifestations of chronic lower limb ischemia against the background of atherosclerosis, who underwent cord blood cell transplantation in combination with conservative therapy. The control group included patients with obliterating atherosclerosis and distal vascular lesions of the lower extremities, received a basic course of conservative therapy. To determine the quality of life before and after cord blood cell transplantation, we used a number of standardized questionnaires, which helped to determine the distance of intermittent claudication and make a subjective assessment of the personal well-being level of patients. To analyze the state of microcirculation in the ischemic limb, we used the laser Doppler flowmetry method. The following changes were observed after the cord blood cell transplantation: an improvement in the general state of patients, a decrease in the degree of ischemia according to Rutherford's classification, an increase in the distance and speed of painless walking during the first 3 months by 5-10%, and 1.3 times in 12 months compared with the control group. The improvement in the microcirculatory parameters of laser Doppler flowmetry was noted from the end of the first to the 12th month after transplantation, which indicated an improvement in microhemodynamics. The use of cord blood cell transplantation in order to stimulate angiogenesis allows one to obtain a long-term positive clinical effect, which manifests itself in the form of an increase in the distance and speed of painless walking, improved working capacity, and improved personal well-being of patients in the physical, psychological and socio-economic field, correlated with laser Doppler flowmetry data and expands the possibilities of successful treatment of patients with chronic ischemia of the lower extremities on the background of obliterating atherosclerosis.

Clinical efficacy of osteopathic correction in the complex treatment of patients with unoperated open-angle glaucoma

2020 ◽  
pp. 97-105
Author(s):  
E. N. Simakova ◽  
O. V. Stenkova
Keyword(s):  
Intraocular Pressure ◽  
Drug Therapy ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Randomized Study ◽  
Main Group ◽  
Control Group ◽  
Open Angle ◽  
Complex Treatment ◽  
Hypotensive Drug

Introduction. Glaucoma is one of the most significant eye diseases. It is often diagnosed, not always amenable to therapy, and can lead to a complete loss of visual functions. In recent years, the method of osteopathic correction has become widespread as one of the effective methods of treatment and rehabilitation of patients with pathologies of various body systems. In the pathogenesis of glaucoma, it is customary to distinguish a dystrophic concept, which considers primary open-angle glaucoma as a result of dystrophic changes in the connective tissue, as well as in the endothelial lining of the trabeculae and Schlemm′s canal, especially destructive changes in mitochondria and the alteration of their functional activity. A vascular concept is also distinguished. According to this concept, the central link in the pathogenesis of glaucoma is circulatory disorder in the ciliary vessels, ocular artery, and major vessels of the head and neck, it can be assumed that osteopathic correction in the treatment of patients with open-angle glaucoma will be pathogenetically substantiated and will have a positive effect on intraocular pressure and trophicity of the optic nerve. The goal of research — to study the influence of in osteopathic correction on the nature of unoperated glaucoma (stage IIA) and to substantiate the possibility of using osteopathic correction in the complex treatment of patients with this pathology.Materials and methods. A prospective controlled randomized study was conducted at 52 city polyclinics, branch 3, Moscow, from January 2018 to January 2019. 40 patients (70 eyes) aged 50 to 75 years with primary open-angle glaucoma IIA stage were examined. At this stage of the disease, patients most often seek medical care and the issue of conservative management is primarily considered. All patients were divided into two groups of 20 people: the main group and the control group. The treatment in the main group included hypotensive drug therapy and osteopathic correction. Patients of the control group received only drug therapy. All patients underwent ophthalmic (visometry, tonometry, perimetry) and osteopathic examination twice: before the treatment and after 3 months.Results. For patients with primary open-angle IIA non-operated glaucoma, regional (most often regions of the head, neck, dura mater) and local (abdominal diaphragm, iliac bones, hip and knee joints) somatic dysfunctions were the most typical. In the main group a statistically significant decrease in the frequency and severity of dysfunctions at all levels was stated. Also, in patients receiving osteopathic correction, a significant decrease in the level of intraocular pressure and perimetric indices was noted. In patients of the control group, no reliable changes in these indicators were obtained.Conclusion. The results obtained indicate that osteopathic correction is clinically effective in the complex treatment of patients with primary open-angle II A glaucoma.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

The effect of probiotics on the level of immunoglobulin A in women with violation of the biocenosis of the vagina

2017 ◽  
pp. 85-88
Author(s):  
O.I. Ostapenko ◽  
◽  
V.P. Kvashenko ◽  
I.K. Akimova ◽  
I.N. Nosova ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Lactobacillus Rhamnosus ◽  
Test System ◽  
Main Group ◽  
Reproductive Age ◽  
Local Action ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Local Immunity ◽  
Serum Iga

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina. Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment. A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets. Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal. Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina. Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

Sign in / Sign up

Export Citation Format

Share Document