scholarly journals The effect of thyroid hormone deficiency on erythropoiesis in dogs

2019 ◽  
Vol 88 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Olga Aniołek
Keyword(s):  
Thyroid Hormone ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Clinical Symptoms ◽  
Cell System ◽  
Hormone Deficiency ◽  
Control Group ◽  
Average Mass ◽  
Packed Red Blood Cells ◽  
Experimental Group

This research aimed to evaluate the effect of thyroid hormone deficiency on the erythrocytic system in dogs. Dogs with clinical symptoms of hypothyreosis such as obesity, hyperpigmentation, and lethargy were selected. The dogs demonstrating breed predisposition to hypothyreosis were incorporated in the analysis: Dachshunds, Retrievers, and mixed-breed dogs. A detailed history was taken and clinical, hormonal, biochemical and haematological blood tests were performed. Peripheral blood samples were taken from 53 dogs. Finally, the dogs with the initial T4 (thyroxine) concentration < 1.3 µg/dl and animals demonstrating clinical improvement after a 2-month therapy with levothyroxine at a dose of 10 µg/kg administeredper ostwo times a day were qualified. The animals between 10 months to 13 years of age were divided into two groups: clinically healthy (control group, n = 35) and dogs presenting clinical symptoms of hypothyreosis (experimental group, n = 18). In this research, the broadly described normocytic normochromic non-regenerative anaemia was not diagnosed in dogs with hypothyreosis. However, a positive correlation between T4 and red blood cell indices such as the average mass of haemoglobin per red blood cell, concentration of haemoglobin in a given volume of packed red blood cells as well as a negative correlation with haematocrit value was discovered in the experimental group after the 2-month therapy with levothyroxine. These results point to the influence of thyroid hormones on erythropoiesis. This observation is partially consistent with other studies, which noted the casual link between the changes in red blood cell system and the function of thyroid in dogs and humans.

scholarly journals The effect of the red blood cell system disorders on the further development and productivity of Holstein calves that had had bronchopneumonia

2021 ◽  
Vol 91 (5) ◽  
pp. 473-481
Author(s):  
Yuriy Alekhin ◽  
◽  
Maksim Zhukov ◽  
Valentina Morgunova ◽  
Yuliya Dronova
Keyword(s):  
Blood Cell ◽  
Clinical Supervision ◽  
Red Blood Cell ◽  
Clinical Symptoms ◽  
Fetal Hemoglobin ◽  
Hypochromic Anemia ◽  
Cell System ◽  
Lactating Cows ◽  
Holstein Calves ◽  
Further Development

The aim of this research was to study the effect of red blood cell system disorders on the further development and productivity of calves that had had bronchopneumonia. The study included 170 Holstein heifers at the age of 180-195 days: healthy heifers (n = 92) and heifers with moderate bronchopneumonia (n = 78). All animals had been under constant clinical supervision for 27 months. Blood sampling was performed in 10 animals from each group at the beginning of the experiment and on day 22, and also when they reached a body weight of 380 kg (the first artificial insemination), 30 days before the planned calving date, and on days 7, 90, 180 of lactation. The blood samples obtained were examined by a hematological analyzer, and the content of fetal hemoglobin was determined by Singer’s method. It was shown that after completion of the course of treatment the clinical symptoms of bronchopneumonia disappeared in calves, but microcytic hypochromic anemia appeared. Further, in these animals an increase was registered in the age of fruitful fertilization by 10.8% and of the insemination index by - 36.7%. After calving and during lactation, the severity of hypochromia decreased, but there was a tendency for macrocytosis, which, combined with increased anisocytosis, indicated hypoplastic anemia. This form of anemia occurred against the background of pathological residual changes after pneumonia due to the depletion of the compensatory potential of the bone marrow and its functional overload during lactation. The operational anemia revealed in lactating cows caused a decrease in milk productivity by 23.2-26.7% (P<0.01).

scholarly journals PO-067 Effects of oral Lycium barbarum juice in red blood cell antioxidant biomarkers and physical function during 8 days of aerobic exercise

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Chaoge Wang ◽  
Xiquan Weng ◽  
Yu Chen ◽  
Jieru Lin
Keyword(s):  
Blood Cell ◽  
Aerobic Exercise ◽  
Red Blood Cells ◽  
Physical Function ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Control Group ◽  
Urine Protein ◽  
Lycium Barbarum ◽  
Experimental Group

Objective Lycium barbarum polysaccharide (LBP) is the main active components of Lycium barbarum, its benefits to anti-aging, vision, kidney, and liver functions. Nevertheless, there is still a scarcity of experimental evidence to support the effect of Lycium barbarum on aerobic exercise.This a randomized controlled trial was observed the effects of oral Lycium barbarum juice in red blood cell antioxidant biomarkers and physical function during 8 days of aerobic exercise. Methods 28 healthy male college students were divided into control group（16）and experimental group（12），and underwent interval running once every other day，total of 8 days. Exercise program: An exercise session includes two 30-minute aerobic exercises at 60%VO2max and a five-minute break. For the duration of the 8 days period, participants exercised one time every other day and the experimental group drank 100ml Lycium barbarum juice (each LBP content 360-440mg%) at bedtime every night. In ninth days, all the experimenters did exhaustive exercise with 80%VO2max on a treadmill with 8°.simultaneous recording of motion duration. The levels of red blood cell SOD, MDA, GSH-PX, serum CAT, serum TAC and other oxygenation stress markers and BLA, Glu, Urea, CK, Urine eight items and other physical function indexes of the subjects were determined before the experiment and after the completion of each intensity exercise. Differences between before and after intervention values were tested using a paired t test.And to compare the mean of outcomes in quantitative variables between the 2 groups, a independent t-test was used. The SPSS software (version 17, SPSS Inc, Chicago, IL, USA) was applied for data analysis and statistical significance was accepted at P < 0.05. Results (1)After 8 days of oral Lycium barbarum juice, the exhaustion time of exercise force in the experimental group was 30.76 ±12.19min, while the control group was 23.64±8.56min. Compared with the control group, the average exercise exhaustion time of the experimental group was prolonged 7.12min. (2)The red blood cell SOD in the two groups after 8 days of aerobic exercise had significant and significant improvement (P < 0.05, P < 0.01), and moreover, the increase of the experimental group was significantly higher than that of the control group (P < 0.05).As well as, the blood erythrocyte GSH-PX and serum TAC were significantly enhanced after the experiment (P < 0.01).It is suggested that increasing the levels of SOD and GSH-PX in vivo is beneficial in scavenging the free radicals produced by body movement. (3)After the 8 days oral Lycium barbarum juice, the decrease of MDA in blood red blood cells in the experimental group was greater than that of the control group (P < 0.01), indicating that the juice of Lycium barbarum could reduce the production of lipid peroxide products. (4) Exhaustion exercise after 8 days of oral Lycium barbarum juice, the physical function indexes of the experimental group, such as BLA, Urea, and CK were reduced. The positive rate of eight urine items was less than that in the control group, 8 in the control group, 2 for bilirubin positive, 3 in the urinary occult blood and 5 in the urine protein, while only 1 in the experimental group were positive for urine protein.   Conclusions Oral Lycium barbarum juice can improve the activity of antioxidant enzymes during aerobic exercise, reduce the formation of lipid peroxides in the body, protect the biological function of red blood cells, improve the physical function and postpone the production of sports fatigue.

scholarly journals Сосудистая проницаемость и микрогемореология при системной склеродермии: возможности метода конъюнктивальной микроскопии

2020 ◽  
pp. 23-28
Keyword(s):  
Systemic Sclerosis ◽  
Blood Cell ◽  
Key Words ◽  
Vascular Permeability ◽  
Red Blood Cell ◽  
Cell Aggregation ◽  
Control Group ◽  
Age Group ◽  
Red Blood Cell Aggregation ◽  
Blood Cell Aggregation

In this article, the authors draw attention of readers to the capabilities of the conjunctival microscopy (CM) the method for studying the processes of vascular permeability and microhemorheology in patients with systemic sclerosis (SS). An original simultaneous examination of 48 patients with SS (mean age 51±1,7 years) and a comparable age group (4,74±2,3 years) of the control group of people without any diseases that might affect microcirculation (MC) by the CM-method was performed. The results demonstrated high informativeness of the CM-method in the diagnostics and assessment of vascular permeability and intravascular red blood cell aggregation (RBCA) in SS. The main changes in MC during SS revealed by the CM-method are the increase in vascular permeability and enhancement of RBCA in all types of microvessels. Key words: vascular permeability, intravascular red blood cell aggregation, conjunctival microscopy, systemic sclerosis.

Transfusion Requirements in Nonmyeloablative Allogeneic Stem Cell Transplantation for Hematologic Malignancies.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4178-4178
Author(s):  
Jason W. Brown ◽  
Darrell J. Triulzi
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Platelet Transfusion ◽  
Transfusion Requirement ◽  
Hematologic Malignancies ◽  
Packed Red Blood Cells ◽  
Transfusion Requirements ◽  
Cell Transplants

Abstract Nonmyeloablative allogeneic stem cell transplants (NM-ASCT) are increasingly being utilized to treat hematologic malignancies in settings where high dose, fully ablative regimens would not be tolerated. We identified 10 male and 13 female patients (n=23) ranging in age from 19 to 63 years old (mean=48) representing all patients whom had undergone NM-ASCT in the last five years. NM-ASCT were performed for acute myelogenous leukemia (n=7), non-Hodgkin’s lymphoma (n=6), Hodgkin’s disease (n=4), multiple myeloma (n=3), myelodysplastic syndrome (n=2), and acute lymphoblastic leukemia (n=1). The majority of patients had undergone at least one previous stem cell transplant (n=16). The mean number of stem cells infused per kilogram at time of transplant was 4.72 X 106 ±1.97 X 106 stem cells and mean time to engraftment was 15.8 ±5.15 days (3 consecutive days with ANC &gt;500). All of the subjects required both platelet and red cell transfusion during the period of analysis. Mean transfusion requirements in the 7 days prior to transplantation were 3.1 ±5.9 units of platelets and 1.2 ±1.5 units of packed red blood cells. Mean platelet transfusion requirements 90 days post transplant were 73.7 ±79.7 units and mean red cell requirements 11.6 ±8.3 units. Total mean transfusion requirements during the time period analyzed were 76.9 ±82.2 units of platelets and 12.8 ±8.9 units of packed red blood cells. There was no significant correlation between the number of stem cells infused per kilogram on day of transplant, the number of previous transplants, or the time to engraftment with red blood cell or platelet transfusion requirements. Our data illustrate a higher proportion of patients requiring platelet transfusions (100% vs. 23%) and red blood cell transfusions (100% vs. 63%) as well as a higher platelet transfusion requirement (median 48 vs. 0 units) and packed red blood cell transfusion requirement (median 11 vs. 2 units) than other authors have reported in a 60 day period in HLA-matched sibling NM-ASCT [Weissinger et al. Blood 98(13):3584-8, 2001]. As is illustrated by the percentage of patients undergoing previous fully ablative stem cell transplants, our patients represent a heavily pretreated population and this may account for the higher transfusion requirements seen in this analysis. Although patients undergoing NM-ASCT receive reduced intensity therapy, in our analysis their transfusion requirements remain substantial.

Indicator Characterizing Carbonyl-Dependent Modification of Erythrocytic Superoxydismutase as a Biochemical Marker of Oxidative Stress in Coronary Heart Disease

Kardiologiia ◽  
2020 ◽  
Vol 60 (5) ◽  
pp. 57-61
Author(s):  
A. K. Tikhaze ◽  
V. Ya. Kosach ◽  
V. Z. Lankin ◽  
A. A. Panferova ◽  
M. D. Smirnova
Keyword(s):  
Oxidative Stress ◽  
Heart Disease ◽  
Stress Intensity ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Oxidative Modification ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Sod Activity ◽  
Stress Marker

Aim To study the oxidative modification of red blood cell Cu,Zn superoxide dismutase (SOD) in patients with ischemic heart disease (IHD) in vivo and in vitro to substantiate the use of a new oxidative stress marker.Material and methods Red blood cell Cu,Zn SOD was measured by depression of nitrotetrazolium blue reduction by the superoxide anion generated in xanthine oxidase xanthine oxidation. Red blood cell Cu,Zn SOD was measured immunochemically. The biochemical study was performed in the control group (patients with low extremity fracture without known history of cardiovascular diseases and hyperlipidemia) and in groups of patients with acute myocardial infarction, stable angina, and decompensated heart failure. For evaluation of oxidative stress intensity in IHD patients, an empirical SOD oxidative modification coefficient (OMCSOD) was proposed, which is a Cu,Zn SOD activity / Cu,Zn SOD content ratio.Results The red blood cell Cu,Zn SOD activity was significantly decreased in all IHD groups compared to the control group. Furthermore, OMCSOD was also considerably decreased in IHD patients, which warrants the use of this biochemical index as an oxidative stress marker.Conclusion It was shown that the Cu,Zn SOD modification was induced by interaction of the enzyme molecules with a natural dicarbonyl, malonic dialdehyde, and OMCSOD can be used for evaluation of oxidative stress intensity in IHD patients.

scholarly journals Red Blood Cell Stiffness Driving Patient Symptoms: A Study of Red Blood Cell Population Rigidity in Sickle Cell Patient Genotype SC Relation to Overlooked Clinical Symptoms

2021 ◽  
Author(s):  
Mark Shamoun ◽  
Mario Gutierrez ◽  
Omolola Eniola-Adefeso
Keyword(s):  
Blood Cell ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Clinical Symptoms ◽  
Severe Disease ◽  
Cell Stiffness ◽  
Severe Phenotype ◽  
Hematological Disease ◽  
Cell Deformability ◽  
Patients At Risk

Sickle cell disease (SCD) is a systemic hematological disease. Various genotypes of the disease exist; however, the two most common include hemoglobin SS (Hgb SS) and hemoglobin SC (Hgb SC) disease. Hgb SC is typically considered a less severe genotype; however, some patients with SC disease still have significant complications. Ektacytometry is utilized to measure red blood cell deformability in sickle cell patients and may help identify patients at risk for severe disease. We described a patient with genotype hemoglobin SC with a more severe phenotype, who we show to have very rigid red blood cells via ektacytometry.

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