The Effect of Invigoration Using Polyethylene Glycol And Ultra  Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.)  Quality After Two Years Storage

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

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Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

Journal of Bioresource Management ◽

10.35691/jbm.1202.0195 ◽

2021 ◽

Vol 8 (3) ◽

pp. 57-66

Author(s):

Ratiba Bousba ◽

Rabah Bounar ◽

Narimene Sedrati ◽

Randa Lekhal ◽

Chourouk Hamla ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Durum Wheat ◽

Crop Production ◽

Germination Rate ◽

Inhibitory Effect ◽

Wheat Genotype ◽

Peg 6000 ◽

Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

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Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211013 ◽

2020 ◽

Vol 21 (10) ◽

Author(s):

ISKANDAR Z. SIREGAR ◽

KARIMA FAUZIAH MUHARAM ◽

Y. ARIS PURWANTO ◽

DEDE J. SUDRAJAT

Keyword(s):

Seed Germination ◽

Storage Time ◽

Physiological Activity ◽

Germination Rate ◽

Seed Viability ◽

Seed Priming ◽

Germination Capacity ◽

Gmelina Arborea ◽

Randomized Design ◽

Germination Characteristics

Abstract. Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564. Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year. On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.

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Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology

Horticulturae ◽

10.3390/horticulturae7070182 ◽

2021 ◽

Vol 7 (7) ◽

pp. 182

Author(s):

Junsik Ahn ◽

Soyeon Oh ◽

Yang Joo Kang ◽

KiBum Kim ◽

Sung-Kwon Moon ◽

...

Keyword(s):

Microbial Community ◽

Seed Germination ◽

Germination Rate ◽

Peanut Seed ◽

Arachis Hypogaea L ◽

Vigor Index ◽

Seedling Biomass ◽

Oak Tree ◽

Fermentation Period ◽

Seedling Characteristics

Peanut (Arachis hypogaea L.) seeds were germinated to investigate the effect of the fermentation period of oak tree sawdust on germination viability and seedling characteristics. Its germination rate, seedling weight, length, and total vigor index were assessed. The seeds were sown in oak tree sawdust fermented for 0, 30, 45, and 60 days. The germination rates of the seeds in fermented sawdust were significantly different. The seeds in the 45-day fermented sawdust produced the heaviest biomass weight (4.6 g) with the longest true leaf (1.7 cm) and hypocotyl (3.4 cm) resulting in the highest total vigor index (925.8). In contrast, seeds in 0-day fermented sawdust had the lowest total vigor index (18.3). Microbiome analysis showed that the microbial community in the sawdust changed as the fermentation progressed, indicating that the microbial community seems to affect seed germination physiology. Taken together, 45-day fermented sawdust is recommended for optimal peanut seed germination and seedling growth.

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Effects of Different Priming Treatments on Seed Germination Properties, Yield Components and Grain Yield of Lentil (Lens culinaris Medik.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319832 ◽

2015 ◽

Vol 43 (1) ◽

pp. 153-158 ◽

Cited By ~ 2

Author(s):

Faruk TOKLU

Keyword(s):

Seed Germination ◽

Grain Yield ◽

Yield Components ◽

Germination Rate ◽

Seedling Emergence ◽

Yield Component ◽

Field Conditions ◽

Distilled Water ◽

Peg 6000 ◽

Positive Effects

An experiment was conducted under laboratory and field conditions in order to evaluate the effects of different priming treatments, specifically KNO3 (1%), KCl (2%), KH2PO4 (1%), ZnSO4 (0.05%), PEG-6000 (20%), IBA (100 ppm), Mannitol (4%), GA3 (100 ppm) and distilled water, on seed germination properties and several agro-morphological plant characteristics of red lentil. Seeds not primed were used as a control. GA3 treatment increased shoot length. The control (non-primed seeds) treatment resulted in increased seedling root number and length. Distilled water, ZnSO4 and control treatments increased germination rate and percentage. In the pot experiments, GA3 treatment increased plant height and seedling emergence rate, whereas KCl treatment improved the number of nodules, as well as root and shoot dry weight when compared to the control. ZnSO4 treatment increased yield components and grain yield in field conditions. The results of this study showed that ZnSO4, GA3 and PEG-6000 seed priming treatments may be useful tools due to their positive effects on germination rate, germination percentage, yield component and grain yield of lentil.

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Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽

10.3390/horticulturae7110490 ◽

2021 ◽

Vol 7 (11) ◽

pp. 490

Author(s):

Saeng Geul Baek ◽

Jin Hyun Im ◽

Myeong Ja Kwak ◽

Cho Hee Park ◽

Mi Hyun Lee ◽

...

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Germination Rate ◽

Seed Viability ◽

Cold Stratification ◽

Dormancy Breaking ◽

Viability Test ◽

Physiological Dormancy ◽

Different Temperatures ◽

Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

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Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

10.21203/rs.3.rs-19878/v1 ◽

2020 ◽

Author(s):

Kaihui Zhai ◽

Guangwu Zhao ◽

Hongye Jiang ◽

Caixia Sun ◽

Jingyu Ren

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Seed Germination ◽

Transgenic Tobacco ◽

Germination Rate ◽

Myb Transcription Factor ◽

Transcription Factor Gene ◽

Factor Gene ◽

Myb Transcription Factors ◽

Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

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PENGGUNAAN Trichoderma Koningii PADA PERKECAMBAHAN SIRSAK (Annona muricata linn)

AGRI-SOSIOEKONOMI ◽

10.35791/agrsosek.15.3.2019.26496 ◽

2019 ◽

Vol 15 (3) ◽

pp. 563

Author(s):

Erida Derita Dalame ◽

Bertje Richard Albert Sumayku ◽

Jeany Polii - Mandang

Keyword(s):

Seed Germination ◽

Plant Protection ◽

Germination Rate ◽

Soaking Time ◽

The North ◽

North Sulawesi ◽

Trichoderma Koningii ◽

Randomized Design ◽

Vigor Index ◽

Two Factors

This study aims to determine the dose of Trichoderma koningii which can induce soursop germination; know the best soaking time that can affect the induction of soursop germination; to know the interaction between soaking time and Trichoderma koningii dose on soursop germination. This research was conducted in April - June 2019 at the Green House of Plant Sciences, Faculty of Agriculture, Sam Ratulangi University, Manado. The material used in this study was soursop seed obtained from Lota Village, Pineleng Tomohon Subdistrict, Trichoderma koningii with 106 solids obtained from the North Sulawesi Province Plant Protection Center Collection, Aquadest, 70% Alcohol, 80% Acetone, sandalready sifter and sterilized. This research was arranged in factorial with a Completely Randomized Design (CRD) consisting of two factors, namely 5 treatments of Trichoderma koningii dose and 4 immersion treatments repeated three times (3x) so that a total of 60 treatment units. Each treatment unituses 10 seeds. The results of this study indicated that the dose of Trichoderma koninggi 200 g without soaking can increase the germination power by 86.67% and the vigor index by 32.96%; Immersion with Koninggi Trichoderma suspension has not been able to induce soursop seed germination rate; Theinteraction between Trichoderma koningii dose and soaking time did not affect the speed of soursop seed germination.*eprm*

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Effects of Matric and Osmotic Priming Treatments on Broccoli Seed Germination

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.3.423 ◽

1996 ◽

Vol 121 (3) ◽

pp. 423-429 ◽

Cited By ~ 17

Author(s):

Lewis W. Jett ◽

Gregory E. Welbaum ◽

Ronald D. Morse

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Root Growth ◽

Growth Rates ◽

Germination Rate ◽

Membrane Integrity ◽

Seed Vigor ◽

Radicle Emergence ◽

The Mean ◽

Peg 8000

Priming, a controlled-hydration treatment followed by redrying, improves the germination and emergence of seeds from many species. We compared osmotic and matric priming to determine which was the most effective treatment for improving broccoli seed germination and to gain a greater understanding of how seed vigor is enhanced by priming. Broccoli (Brassica oleracea L. var. italica) seeds were osmotically primed in polyethylene glycol (PEG 8000) at -1.1 MPa or matrically primed in a ratio of 1.0 g seed:0.8 g synthetic calcium silicate (Micro-Cel E):1.8 ml water at -1.2 MPa. In the laboratory, germination rates and root lengths were recorded from 5 to 42C and 10 to 35C, respectively. Broccoli seeds germinated poorly at >35C. Root growth after germination was more sensitive to temperatures >30C and <15C than radicle emergence. Matric and osmotic priming increased germination rate in the laboratory, greenhouse, and field. However, matric priming had a greater effect on germination and root growth rates from 15 to 30C. Neither priming treatment affected minimum or maximum germination or root growth temperatures. Both priming treatments decreased the mean thermal time for germination by >35%. The greater germination performance of matrically primed seeds was most likely the result of increased oxygen availability during priming, increased seed Ca content, or improved membrane integrity.

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Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.474-476.36 ◽

2011 ◽

Vol 474-476 ◽

pp. 36-39 ◽

Cited By ~ 1

Author(s):

Yong Dong Sun ◽

Xin Zheng Li ◽

He Lian Yang ◽

Li Sun

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Seed Priming ◽

Distilled Water ◽

Nacl Solution ◽

Peg 6000 ◽

Germination Process ◽

Germination Characteristics ◽

Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

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GERMINATION OF STORED SEEDS OF FOUR TREE SPECIES FROM THE TROPICAL DRY FOREST OF MORELOS, MEXICO

Botanical Sciences ◽

10.17129/botsci.96 ◽

2014 ◽

Vol 92 (2) ◽

pp. 281 ◽

Cited By ~ 3

Author(s):

Martha Cervantes ◽

Eliane Ceccon ◽

Consuelo Bonfil

Keyword(s):

Seed Germination ◽

Seed Size ◽

Storage Time ◽

Germination Rate ◽

Tropical Dry Forest ◽

Dry Forest ◽

Tropical Dry Forests ◽

Germination Capacity ◽

Dry Forests ◽

And Storage

Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in Acacia bilimekii, Haematoxylum brasiletto, Lysiloma acapulcense, and L. divaricatum. Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two Lysiloma species, but in A. bilimekii there were large differences among provenances. Mean germination capacity was low in H. brasiletto after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests.

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