scholarly journals Pengaruh Ketinggian Tempat Tumbuh Terhadap Kandungan Fitokimia dan Antioksidan Ekstrak Akar Sawi Langit (Vernonia cinerea L.)

2021 ◽  
Vol 5 (2) ◽  
pp. 84-93
Author(s):  
Sri Lestari ◽  
Riska Desi Aryani ◽  
Dian Palupi
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Herbaceous Plant ◽  
Central Java ◽  
Antioxidant Content ◽  
Ic50 Value ◽  
The Tropics ◽  
Positive Results ◽  
Vernonia Cinerea

This study aims to determine the effect of altitude on the phytochemical and antioxidant content of sawi langit (Vernonia cinerea L.)  root. Sawi langit is a herbaceous plant that has potential as a medicinal plant. This plant can thrive in the tropics. In this study, sawi langit were taken from three altitudes, namely <400 masl, 401-800 masl and >801 masl in the Banyumas, central Java. The sample used was sawi langit root which was extracted using 3 types of solvents (methanol, ethyl acetate and n-hexan). Phytochemical tests showed positive results of the content of flavonoid, alkaloids, tannins, terpenoids and saponins compounds in the extract of sawi langit root from the three altitude with differences effectiveness of each solvent. At an altitude of <400 masl and extraction using methanol resulted  strong antioxidant activity with an IC50 value 78.56 ± 3.2  μg/ml compared to an altitude of 401-800 masl and >801 masl

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

2011 ◽  
Vol 76 (10) ◽  
pp. 1379-1386
Author(s):  
A Aziz-Ur-Rehman ◽  
Sehrish Gulzar ◽  
Muhammad Abbasi ◽  
Tayyaba Shahzadi ◽  
Tauheeda Riaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ic50 Value

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ? 0.71 ?g mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1? 0.92 ?g mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ? 27.1 ?g TE mL-1), inhibition of lipid peroxidation (60.93 ? 0.84 % at 500 ?g mL-1) and total phenolic content (95.5 ? 0.05 ?g GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions.

scholarly journals Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium

2018 ◽  
Vol 10 (1) ◽  
pp. 87-94
Author(s):  
Nuniek Ina Ratnaningtyas ◽  
Purnomowati Purnomowati ◽  
Endang Sri Purwati ◽  
Aisyah Tri Septiana ◽  
Nuraeni Ekowati ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Research Result ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Antioxidant Potential ◽  
Complete Medium ◽  
Central Java ◽  
Local Resources ◽  
Ic50 Value ◽  
Better Than

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Azimatur Rahmi ◽  
Tika Afriani ◽  
Linda Hevira ◽  
Wike Widiawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Activity Assay ◽  
Ic50 Value

The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.

scholarly journals KAJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN TEMBELEKAN (Lantana camara L) BERDASARKAN TINGKAT KEPOLARAN PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (3) ◽  
Author(s):  
Oktavian Mangela ◽  
Ahmad Ridhay ◽  
Musafira Musafira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Antioxidant Compounds ◽  
Ic50 Value

The investigation about the antioxidant activity of tembelekan (Lantana camara L) leaf extract based on level polar solvent . Has been done the aim of this study was to determine the IC50 value and to know the content of antioxidant compounds from tembelekan leaf extract based on the level of polar solvent the extraction of tembelekan leaf was done with maceration method . The result showed that ethyl acetate extract of tembelekan leaf has the highest antioxidant activity with IC50 value was 71,70 ppm. Keywords: Tembelekan ( Lantana Camara L ) , IC50 , Antioxidant , DPPH

scholarly journals UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Firdaus Firdaus ◽  
Jalifah Latip
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Vitamin ◽  
Activity Assay ◽  
Immersion Method ◽  
Ic50 Value ◽  
Measured Absorption ◽  
Hexane Extracts

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of  T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL. 

scholarly journals Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

2019 ◽  
Vol 7 (1) ◽  
pp. 86-91
Author(s):  
Fredryk W Mandey ◽  
Endah Handayani ◽  
Wahyuni Eka Nanda ◽  
Alfian Noor
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Steroid Alkaloid ◽  
South Sulawesi ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Positive Results

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

scholarly journals Penyarian Konstituen Organik Daging Buah Gaharu (Aquilaria malaccensis Lamk.) dengan Metode Maserasi Berbantu Microwave dan Uji Aktivitas Sebagai Antioksidan

KOVALEN ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Imran ◽  
Nurlian ◽  
L.A. Kadir ◽  
La Agusu ◽  
Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Fruit Pulp ◽  
Microwave Assisted ◽  
Organic Constituents ◽  
Aquilaria Malaccensis ◽  
Ic50 Value

This study was conducted to determine the organic constituents and to test the antioxidant activity and toxicity of gaharu fruit pulp extract (Aquilaria malaccensis Lamk.). Gaharu fruit pulp extract was obtained through Microwave Assisted Extraction (MAE) method and then extracted with a solvent with different polarity, namely ethyl acetate and n-hexane. The obtained yield of ethyl acetate extract of the gaharu fruit pulp was 40.827% w/v, whereas 0% w/v in n-hexane extract, therefore, no further test for n-hexane extract, so that the n-hexane extract was not tested for the next stage. The results of the organic constituents of the ethyl acetate extract of gaharu fruit pulp showed flavonoids, saponins, glycosides, phenols and tannins. Antioxidant activity testing was carried out using the DPPH method with vitamin C as a positive control. The results of the antioxidant activity test showed that IC50 of ethyl acetate extract of gaharu fruit pulp and vitamin C were 143.789ppm and 13.797ppm, respectively. Extracts from microwave-assisted maceration that were partitioned with ethyl acetate solvent were categorized into moderate antioxidants because the IC50 value was between 101-150ppm, while vitamin C was categorized as a strong antioxidant because the IC50 value was between 0-100ppm. Toxicity testing was also carried out on ethyl acetate extract of gaharu fruit pulp using the BSLT method. The results of the toxicity test of the ethyl acetate extract of gaharu fruit pulp showed activity with an LC50 value of 11.282ppm. Based on this research, the ethyl acetate extract of gaharu fruit pulp is considered to have an anticancer potential.

scholarly journals Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 198-205
Author(s):  
Nur Faidah ◽  
Nurhaeni ◽  
Ahmad Ridhay ◽  
Jusman ◽  
Abd. Rahman Razak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Allium Cepa ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Root Extract ◽  
Polar Solvents ◽  
Ic50 Value ◽  
Dpph Method ◽  
Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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