Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.

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Identification and Heme Polymerization Inhibition Activity (HPIA) Assay of Ethanolic Extract and Fraction of Temu Mangga (Curcuma mangga Val.) Rhizome

Eksakta Jurnal Ilmu-Ilmu MIPA ◽

10.20885/eksakta.vol1.iss1.art10 ◽

2020 ◽

Vol 20 (1) ◽

pp. 64-72

Author(s):

Dhina Fitriastuti ◽

Tatang Shabur Julianto ◽

Annisa Wahyu Nur Iman

Keyword(s):

Ethyl Acetate ◽

Antimalarial Activity ◽

Ethanolic Extract ◽

Soxhlet Extraction ◽

Inhibition Activity ◽

Ic50 Value ◽

Ic50 Values ◽

Vacuum Liquid Chromatography ◽

Active Substances ◽

Better Than

Curcuma mangga Val. is one of Indonesian herbs from Zingiberaceae family that is under explored and could contain potentially active substances to serve as an antimalarial. This research intends not only to examine the antimalarial activity by means of heme polymerization inhibitor mechanism by using the ethanolic extract and fraction of Curcuma mangga Val. but also to identify its compound classification. The extract of temu manga was obtained by Soxhlet extraction method using ethanol solvent followed by fractionation using Vacuum Liquid Chromatography with solvent sequence n-hexane, n-hexane: ethyl acetate (2:1), ethyl acetate and ethanol. The extract and fraction were analyzed by using LC-MS and GC-MS. Activities of hem polymerization inhibition showed by IC50 values which were obtained from analysis of relationship between concentration sample and the percentage of inhibition using the PROBIT on statistical software. The result of HPIA assay shows that the IC50 value of ethanolic extract and ethanolic fraction of Curcuma mangga Val. rhizome are 2.273 and 1.479 mg/mL, respectively. It clearly shows that the heme polymerization inhibition activity of ethanolic fraction relatively better than that of ethanolic extract. Phytochemical screening determines the ethanolic extract contains saponin, terpenoid, and phenol while the ethanolic fraction contains terpenoid. Thus, terpenoid compound is presumed to be the inhibitor of heme polymerization. The results of analysis with LC-MS and GC-MS showed that the active compounds suspected to inhibit heme polymerization in ethanolic extract and fraction were (E) -labda-8 (17), 12-dien-15,16-dial and di-n-octyl phthalate, respectively. Keywords: antimalarial, Curcuma mangga Val., heme polymerization

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Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v4i1.4707 ◽

2015 ◽

Vol 4 (1) ◽

pp. 21

Author(s):

Nyi Mekar Saptarini ◽

Irma Erika Herawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Ficus Benjamina ◽

Water Fraction ◽

Free Radical Formation ◽

Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as Ficus benjamina and Annona reticulata. The study aimed to compare the antioxidant activity of extracts and fractions of Ficus benjamina and Annona reticulata leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC50 values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC50 value of Ficus benjamina was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC50 value of Annona reticulata was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the Ficus benjamina water fraction was the best antioxidant compared to other extract and fraction.

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Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

Journal of Chemistry ◽

10.1155/2013/250739 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Muhammad Asam Raza ◽

Rukhsana Kausar ◽

Faraz Ali Rana ◽

Muhammad Danish ◽

Durre Shahwar ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Natural Antioxidants ◽

Stem Bark ◽

Antioxidant Potential ◽

Total Phenolic ◽

Strong Correlations ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

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Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

International Journal of Public Health Science (IJPHS) ◽

10.11591/.v4i1.4707 ◽

2015 ◽

Vol 4 (1) ◽

pp. 21

Author(s):

Nyi Mekar Saptarini ◽

Irma Erika Herawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Ficus Benjamina ◽

Water Fraction ◽

Free Radical Formation ◽

Annona Reticulata

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PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33524 ◽

2019 ◽

pp. 1-6

Author(s):

FONA QORINA ◽

ADE ARSIANTI ◽

QOTRUNNADA FITHROTUNNISA ◽

NADZILA ANINDYA TEJAPUTRI

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Folk Medicine ◽

Parasitic Infection ◽

Ethanolic Extract ◽

Positive Control ◽

Annona Muricata ◽

Tropical Plant ◽

Ic50 Value ◽

Dpph Method

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

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Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

Stamford Journal of Pharmaceutical Sciences ◽

10.3329/sjps.v3i1.6802 ◽

1970 ◽

Vol 3 (1) ◽

pp. 68-74 ◽

Cited By ~ 1

Author(s):

Fatema Tuz Zohera ◽

Md Razibul Habib ◽

Mohammad Zafar Imam ◽

Md Ehsanul Hoque Mazumder ◽

Md Sohel Rana

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Reducing Power ◽

Antioxidant Potential ◽

Dependent Manner ◽

Ic50 Value ◽

Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

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TOTAL PHENOLIC CONTENTS, ANTIOXIDANT, ANTICANCER AND ANTIDIABETIC PROPERTIES OF Myrmecodia tuberosa (RUBIACEAE)

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v9i3.1007 ◽

2013 ◽

Vol 9 (3) ◽

pp. 2000-2004

Author(s):

Saidi Rasemi ◽

Dr Heng Yen Khong ◽

Rohaya Ahmad ◽

Mizaton Hazizul Hasan

Keyword(s):

Ethyl Acetate ◽

Ethanolic Extract ◽

Bark Extract ◽

Total Phenolic ◽

Cytotoxicity Test ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Hela Cell Lines ◽

Ic50 Value ◽

Cancer Agent

Myrmecodia tuberosa, locally known as “Sarang Semut”, belongs to the Rubiaceae family. The aim of the study is to investigate the total phenolic contents and bioactivity of the crude extract of M. tuberosa. The tuber, bark and leaves of the plant were cut, washed and air-dried. The plant was extracted with ethyl acetate and ethanol to yield EtOAc and EtOH crudes. The crudes were then tested for antioxidant 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay, α-glucosidase assay for anti-diabetic activity and [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] MTT Assay for cytotoxicity test. The results showed that bark ethanolic extract demonstrated the highest DPPH scavenging of 95.16% while other crudes were ranging from 92.77% to 94.55% scavenging of DPPH compared to ascorbic acid. The tuber ethyl acetate extract demonstrated the highest inhibition of α -glucosidase enzyme with 72.58% while other crudes were ranging from 63.27% to 72.46% inhibition. In addition, the ethanolic tuber extract exhibited the strongest cytotoxicity against Human colorectal Cancer (HT-29) and Cervical Cancer (HeLa) cell lines with the IC50 value of 16 µg/mL and 14 µg/mL respectively. Meanwhile, the ethanolic bark extract exhibited the strongest cytotoxicity against Human Breast Cancer (MCF-7) with IC50 value of 6.0µg/mL. Furthurmore, the tuber extracts contained the highest phenolic content with 1087mg GAE/ g extract compared to the leaves and barks of the plants. The findings suggest that the whole part of M. tuberosa is a potential natural source for anti-oxidative, anti-diabetic and anti-cancer agent.

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ASSESSMENT OF PHYSICOCHEMICAL PROPERTIES AND PHYTOCHEMICAL CONSTITUENTS ALONG WITH ANTIBACTERIAL POTENTIAL OF TARENNA POLYCARPA LEAF FRACTIONS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23641 ◽

2018 ◽

Vol 11 (4) ◽

pp. 78

Author(s):

Bunga Rimta Barus ◽

Panal Sitorus ◽

Masfria Masfria

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Physicochemical Analysis ◽

World Health ◽

Phytochemical Screening ◽

Phytochemical Constituents ◽

Health Organization ◽

Better Than

Objective: The aim was to investigate the physicochemical analysis, preliminary phytochemical screening and further evaluation of its antibacterial activity from morbesi-besi (Tarenna polycarpa) leaf ethyl acetate and hexane fractions (HF).Methods: The physicochemical parameters were carried out as per the World Health Organization (WHO) guideline from its ethanolic extract. The phytochemical screening was performed as per Indonesia Pharmacopoeia and conventional method on its ethyl acetate and HF. Ethyl acetate and HF at different concentration were evaluated using agar well diffusion assay.Result: The plant was found to be free from contaminations. Morbesi-besi leaf fractions are composed of flavonoids, glycosides, saponins, tannins, steroids, and triterpenoids. The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The antibacterials assay showed that ethyl acetate fraction was better than HF from the inhibition zone.Conclusion: From the outcomes of this study, morbesi-besi leaf (T. polycarpa) possess potential antibacterial activity.

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Pengaruh Ketinggian Tempat Tumbuh Terhadap Kandungan Fitokimia dan Antioksidan Ekstrak Akar Sawi Langit (Vernonia cinerea L.)

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2021.5.2.84-93 ◽

2021 ◽

Vol 5 (2) ◽

pp. 84-93

Author(s):

Sri Lestari ◽

Riska Desi Aryani ◽

Dian Palupi

Keyword(s):

Antioxidant Activity ◽

Medicinal Plant ◽

Ethyl Acetate ◽

Herbaceous Plant ◽

Central Java ◽

Antioxidant Content ◽

Ic50 Value ◽

The Tropics ◽

Positive Results ◽

Vernonia Cinerea

This study aims to determine the effect of altitude on the phytochemical and antioxidant content of sawi langit (Vernonia cinerea L.) root. Sawi langit is a herbaceous plant that has potential as a medicinal plant. This plant can thrive in the tropics. In this study, sawi langit were taken from three altitudes, namely <400 masl, 401-800 masl and >801 masl in the Banyumas, central Java. The sample used was sawi langit root which was extracted using 3 types of solvents (methanol, ethyl acetate and n-hexan). Phytochemical tests showed positive results of the content of flavonoid, alkaloids, tannins, terpenoids and saponins compounds in the extract of sawi langit root from the three altitude with differences effectiveness of each solvent. At an altitude of <400 masl and extraction using methanol resulted strong antioxidant activity with an IC50 value 78.56 ± 3.2 μg/ml compared to an altitude of 401-800 masl and >801 masl

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Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants

Hemijska industrija ◽

10.2298/hemind140830013j ◽

2016 ◽

Vol 70 (1) ◽

pp. 99-106 ◽

Cited By ~ 1

Author(s):

Violeta Jakovljevic ◽

Jasmina Milicevic ◽

Gorica Ðelic ◽

Miroslav Vrvic

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Reducing Power ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Total Phenolic ◽

Scavenging Activity ◽

Flavonoid Content ◽

Dpph Scavenging

The antioxidant activity, total phenolic and flavonoid content of ethanolic, acetone and ethyl acetate extracts of Ruscus hypoglossum L. and Ruscus aculeatus L. (aerial parts) from Serbia were investigated in this paper. The best total antioxidant capacity (23.329 ?g AA g-1) and the highest DPPH scavenging activity (IC50 = 182.54 ?g mL-1) were found in acetone and ethyl acetate extract of R. aculeatus L. Ethanolic extract of R. hypoglossum L. showed the highest ABTS radical cation scavenging activity (IC50 = 3.04 ?g mL-1) as well as reducing power (IC50 = 0.143 ?g mL-1). The best inhibitory activity against lipid peroxidation (IC50 =651 ?g mL-1) and the best ferrous ion chelating ability (IC50 = 110 ?g mL-1) were found in acetone and ethyl acetate extract of R. hypoglossum L. The highest total phenolic (8.569 mg GAE g-1) and flavonoid content (0.136 mg RU g-1) were found in ethanolic and acetone extract of R. hypoglossum L. and R. aculeatus L, respectively.

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