scholarly journals Pertumbuhan Bibit Salak [Salacca zalacca (Gaertner) Voss] Pondoh yang Diinokulasi dengan Isolat Bakteri Potensial di Tanah Regosol Darmaga

2015 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Fahrizal Hazra

<p>ABSTRACT</p><p>Snake fruit [Salacca zalacca (Gaertner) Voss] Pondoh is one of important fruits in Indonesia. The objective of the study was to determine the  influence of some selected potential microbes such as Phosphate Solubilizing Bacteria, Cellulolytic Bacteria, and  N2-fixing Bacteria (Azospirillum)  ongrowth of seedling  of  snake  fruit  Pondoh.  A  study  was  conducted  in  the nursery. The  experiment used  a  completely  randomized  design  with  treatments namely:  K0  (without  inoculum);  A1 (Azospirillum inoculum dose 5 ml), A2 (inoculum 25 ml); F1 (BPF inoculum dose 5 ml), F2 (dose 25 ml); S1; BPS inoculums (dose 5 ml), S2 (dose 25 ml); C1 (mixed bacterial inoculum A+F+S dose 5 ml), and C2 (A+F+S dose 25 ml). Plant height, P, C/N soil ratio, N were measured in 10 months for observing the impact of treatments. Results showed that the effect of inoculation of A2 to the amount of  N-total  (0.99%)  was significantly  different  compared  to  soil  with  K0;  and  not significantly different  from  the  amount  of  N-total  inoculation  A1,  F1,  F2, S1,  S2,  C1,  and  C2.  F2  increased availability of P (20.79 ppm) in the soil higher than  K0, A1, F1; and not significantly different from A2, S1, S2, C1, and C2.  K0 treatment gave  C/N ratio of soil significantly different from A1, A2, F1, F2, S1, S2, C1 and C2; BPS which showed activity in overhauling C-organic in the soil. Inoculation A2  and F2  increased  plant  height  15.48% and 9.31%  higher  than  the  control.  Inoculation  of  C2 differed  significantly  in improving  the  fresh  weight  compared  to  control;  and  not significantly different  from  A1,  A2,  F1,  F2,  S1,  S2,  and  C1  and  able  to increase  the  dry  weight  of  71.12% compared to the control.</p><p>Keywords: Azospirillum, Cellulolytic Bacteria, Phosphate Solubilizing Bacteria, Snake Fruit</p><p> </p><p>ABSTRAK</p><p>Salak Pondoh [Salacca zalacca (Gaertner) Voss] merupakan tanaman buah bernilai tinggi dan diminati  masyarakat.  Budidaya  salak  diawali  dengan pembibitan.  Penelitian  ini  bertujuan  menguji pengaruh  bakteri  potensial hasil  isolasi  dan  seleksi  dari  bakteri  pelarut  fosfat  (BPF), bakteri perombak  selulosa  (BPS),  dan  bakteri  pemfiksasi  N2  (Azospirillum) dalam  pertumbuhan  bibit tanaman  salak pondoh. Penelitian ini menggunakan rancangan acak lengkap satu faktor yang terdiri atas: K0 (tanpa inokulum/Kontrol);  A1  (inokulum  Azospirillum  5  ml), A2 (25  ml inokulum);  F1 (inokulum BPF dosis 5 ml), F2 (dosis 25 ml); S1 (inokulum BPS dosis 5  ml), S2 (dosis 25 ml); C1 (inokulum campuran A+F+S dosis 5 ml), dan C2 (dosis 25 ml).  Pengamatan dilakukan selama 10 minggu  sampai  bibit salak  Pondoh  memiliki dua daun.  Hasil penelitian menunjukkan  inokulasi A2dan S2  nyata  jumlah N-total (0.99%) di dalam tanah dibandingkan  dengan K0; tetapi  tidak berbeda nyata dibandingkan dengan perlakuan lain. Inokulasi F2 nyata meningkatkan P tersedia (20.79 ppm) di dalam tanah dibandingkan dengan K0, A1, F1; dan tidak berbeda nyata dengan A2, S1, S2, C1, dan C2. Induksi dengan seluruh isolat nyata terhadap C/N rasio tanah; yang menunjukkan adanya aktivitas BPS dalam  merombak C-organik  di dalam tanah.  Inokulasi  A2  dan  F2  mampu meningkatkan tinggi tanaman 9.31% dan 15.48% lebih  besar  dibandingkan kontrol. Inokulasi C2 nyata meningkatkan bobot basah bibit salak pondoh dibandingkan kontrol; tetapi tidak berbeda nyata dengan perlakuan lain serta mampu meningkatkan bobot kering 71.12% dibandingkan kontrol.</p><p>Kata kunci: Azospirillum, bakteri pelarut fosfat, bakteri perombak selulosa, Salak Pondoh</p>

2015 ◽  
Vol 2 (1) ◽  
pp. 609 ◽  
Author(s):  
Sri Widawati

<p>This study aimed to determine the extent of phosphate solubilizing bacteria resistant to salinity and still be able to provide P for paddy plant. Research using completely randomized design with fertilizer treatments: <br />(A) Bakteri Aerobacter aerogenes + Azotobacter indicus (B) Bakteri Bacillus thuringiensis + B. megaterium <br />+ Pseudomonas fluorescens, (C) Bakteri Nocardia mesentrica + Spirillum lipoferum, (D) Mix bakteri Pseudomonas fluorescens, Bacillus thuringiensis, B. megaterium, Nocardia mesentrica, Aerobacter aerogenes, Spirillum lipoferum, dan Azotobacter indicus, and (E) control (whitout inoculant), and salinity (NaCl): (1) 0% (0 grams / 7 kg soil), (2) 0.1% (7 grams / 7 kg soil), (3) 0.2% (14 g / 7 kg soil), (4) 0.3% (21 g / 7 kg soil), and (5) 0 , 4% (28 g / 7 kg soil). Thirty and one hundred days after transplanting (DAT), and then measured plant height, number of tillers, number and dry weight of whole grain paddy. The results showed that 0,1 % (7 gram/7 kg tanah) salinity is very good for the growth, activity and effectiveness of phosphate solubilizing bacteria and production of paddy, but 0.4% salinity (28 gr/7kg land) is still safe on the growth, activity and effectiveness of phosphate solubilizing bacteria (Pseudomonas fluorescens, Bacillus thuringiensis, <br />B. megaterium, Nocardia mesentrica, Aerobacter aerogenes, spirillum lipoferum and Azotobacter indicus) as biofertilizer or growth promoting rhizobacteria on growth and production of paddy.</p><p><br /><strong>Keywords</strong>: Salinity, Phosphate solubilizing bacteria, Paddy</p>


2018 ◽  
Vol 23 (1) ◽  
pp. 51 ◽  
Author(s):  
Tintin Suhartini ◽  
Try Zulchi

<p><strong>Abstract</strong></p><p>The research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&amp;#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage &lt;50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage &lt;70%, and 82 accesions were sensitive to highly-sensitive.</p><p><strong>Abstrak</strong></p><p>Penelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &amp;plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati &lt;50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati &lt;70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.</p>


Jurnal Agro ◽  
2022 ◽  
Vol 8 (2) ◽  
pp. 164-177
Author(s):  
Elis Kartika ◽  
Gusniwati Gusniwati ◽  
Made Deviani Duaja

Grafting bibit kopi memiliki tujuan menghasilkan tanaman dengan karakteristik terbaik dari dua varietas kopi yang disambungkan. Kopi robusta digunakan sebagai batang bawah, karena lebih tahan terhadap kondisi yang tidak menguntungkan di lahan gambut. Upaya peningkatan ketahanan batang bawah dapat memanfaatkan mikoriza dan disambungkan dengan kopi liberika sebagai batang atas. Penelitian ini bertujuan untuk  mendapatkan bibit kopi Liberika unggul hasil grafting dengan kopi Robusta bermikoriza serta mendapatkan panjang entres kopi Liberika yang mampu meningkatkan pertumbuhan bibit kopi Liberika hasil sambung pucuk. Percobaan ini menggunakan Rancangan Acak Lengkap faktorial dengan dua faktor dan empat ulangan. Faktor pertama adalah inokulasi mikoriza (tanpa aplikasi mikoriza dan aplikasi mikoriza gabungan Glomus sp-1a dan Glomus sp-3c) dan  faktor kedua berupa panjang entres (10, 15, 20 dan 25 cm). Parameter yang diamati adalah persentase keberhasilan sambungan, waktu pecah tunas, pertambahan tinggi bibit, pertambahan jumlah daun, jumlah tunas, bobot kering tunas, dan infeksi mikoriza. Hasil penelitian menunjukkan respons bibit kopi Liberika hasil grafting dengan bibit kopi Robusta bermikoriza terbaik diperoleh pada panjang entres 15 cm, sedangkan yang disambungkan dengan Robusta tidak bermikoriza diperoleh pada panjang entres 25 cm. Pertumbuhan bibit kopi Liberika hasil grafting dengan bibit kopi Robusta terbaik pada berbagai panjang entres diperoleh pada kopi Robusta bermikoriza.” The main goal of coffee grafting is to create a crop with the best characteristic of two coffee varieties in one plant. Robusta coffee is used as the rootstock, which is more resistant to constraints and unfavorable conditions in the peatland. The effort to increase rootstock resistance is inoculated by mycorrhizae and grafted with Liberica coffee as the scion. This study aimed to obtain the best scion length in order to increase the growth of Liberica coffee with Robusta coffee as the inoculated rootstock. The experiment used factorial completely randomized design with the first factor was mycorrhizae inoculation (without inoculation and inoculation of Glomus sp-1a and Glomus sp-3c combination) and the second factor was  the length of scion (10, 15, 20 and 25 cm). The variables observed were the percentage of success grafted plants, shoot break time, growth (plant height, number of leave, number of shoots, and shoot dry weight) and mycorrhizae infection. The results showed that the plant inoculated by mycorrhizae and scion lenght of 15 cm gave the best percentage of the success graft, shoot break time, and growth of scion. While, the root stock without inoculation showed the best result with the scion length 25 cm. The best growth of grafted plant was obtained in all scion length with mycorrhizae inoculation. 


2015 ◽  
Vol 2 (1) ◽  
pp. 54-65
Author(s):  
Nurul Hidayati ◽  
Asro Laelani Indrayanti

The purpose of this research is gaining influence boiler ash on growth and yield of tomatoes, (4) obtain optimal dosing of boiler ash is curuently applied to some media. This research was conducted at Jl. Manjuhan Palangkaraya. The time of this study will last for 4 months This study uses a completely randomized design factorial 2 factors. The first factor is the dose Abu boiler with a 5 stage treotment (without ash, 5 ton.ha-1, l0 ton ha-1, 15 ton.ha-1 and 20 ton.ha-1 . The second factor is the type of media with 3 levels of treatment (Soil sand, soil Podsolid and Peat. The treatment combinotion obtained as-mony as 15 combinations of treatment, with a repeat 3 times. The survey results reveoled that (1) the interaction of growth media and boiler ash dose showed significantly different results against 2,4 dan 6-wieks after planting variables plant height, amount of leaves on the age, 2, 4 and 6 weeks after, plantig, stem diameter at 4 weeks afier planting, and shoot dry weight lastest research. Treatment combination boiler ash 15 ton / ha and peat media (treatment b4t3) provide significant variahles plant height, leaf amount, diameter of stock, and shoot dry weight.


2018 ◽  
Vol 5 (3) ◽  
Author(s):  
A F A Putra ◽  
N D Hanafi ◽  
Hamdan ◽  
M Tafsin ◽  
R E Mirwandhono

This study aims to determine effects of production of Brachiaria decumbens grass on different drought stress treatments. Research was conducted at the Greenhouse Faculty of Agriculture University of North Sumatera from Desember 2016 until April 2017. The design used was Completely Randomized Design (CRD), with three treatments and seven replications. The treatment consisted of three levels of drought stress which were control (100% field capacity=fc), medium drought stress (25% fc), and heavy drought stress (50% fc). The variable were fresh weight production, dry weight production and plant height. The results showed that drought stress treatment control (100% fc) significantly (P<0,05) affect fresh weight production, dry weight production, but not plant height. It is concluded that increasing water volume of field capacity increase production Brachiaria decumbens grass.


2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Ekal Kurniawan ◽  
Arifah Rahayu ◽  
Yanyan Mulyaningsih

Katuk (Sauropus androgynus (L.) Merr.) is an indigenous leafy vegetable whose growth is affectedby nitrogen element. This study was aimed at assessing the agronomic performance of four katukaccessions grown with various rates of cattle urine. A factorial completely randomized design wasused. The first factor was katuk accession consisting of four levels, namely Pandeglang, Cianjur,Sukabumi, Leuwiliang, and Kemang. The second factor was cattle urine rates consisting of 5 levels,namely 0%R cattle urine (0.0 ml/plant), 50%R cattle urine (468.75 ml/plant), 100%R cattle urine(937.50 ml/plant), 150% R cattle urine (1406.25 ml/plant), and 100%R urea (8.2 g/plant). Thegrowth and productivity of katuk of Cianjur accession had the highest number of buds, total lengthof buds, number of leaves, number of leaflets, fresh weight, and dry weight. The utilization of 50%R,100%R, and 150%R of cattle urine was found to have higher fresh and dry weight than did theutilization of 0%R cattle urine and 100%R urea. The best quality katuk was Cianjur accessionwhich was found to have the highest vitamin C and chlorophyll contents. No significant effects ofall treatments were found in all vegetative variables except plant height and root fresh and dryweight. It was concluded that cattle urine could be used as a urea substitute.Key words: Sauropus androgynus, number of buds, dry weight, indegenous plant


2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Sy. Hasan Agil Riza Linda Rafdinal

Batik spinach (Amaranthus tricolor L.) is a plant that is rich in nutrients and can be used as diabetes medicine and dysentery. Therefore, increasing the growth of batik spinach plants needs to be done, one of which is the use of organic fertilizers, which can be used as rabbit biourin. The use of rabbit urine is done because rabbit urine has a high nutrient content compared to other livestock. The aim of the study was to determine the effect of biourin on the growth of batik spinach (Amaranthus tricolor L.). The study used a Completely Randomized Design (CRD) with 5 levels of concentration treatment consisting of controls, 25 ml / L, 30 ml / L, 35 ml / L, 40 ml / L, with 5 replications. The results showed that the administration of rabbit biourin significantly affected plant height, number of leaves, wet weight, dry weight and ratio of roots / canopy. The concentration of 25 ml / L gave the highest effect on the parameters of plant height, leaf number, root / crown ratio, wet weight, and dry weight.


2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Nurul Huda ◽  
Mukarlina Mukarlina ◽  
Elvi Rusmiyanto Pancaning Wardoyo

White jabon (Anthocephalus cadamba (Roxb.) Miq) are plants with high economic value. It can be propagated vegetatively using its shoot cuttings. One of the way to enhance the growth of shoot cuttings by submersion in green bean sprout extract was expected to produce shoot cuttings with better growth. This study aimed to determine the submersion effect of white jabon (A. cadamba) shoot cuttings in green bean (Vigna sinensis) sprout extract. The Completely Randomized Design (CRD) consisted of 5 levels of treatment were used. The cuttings were submerged in extract concentrations of 0%, 25%, 50%, 75%, and 100%. The study parameters of number of roots and leaves, plant height, wet and dry weight were observed. The results showed that the concentration treatment with 75% green bean sprout extract produced 9,25±2,50 root strands, 5,75±0,50 leaves, plant height of 20,13±1,11 cm, wet weight of 12,39±3,62 g, and dry weight of 1,51±0,42 g.


2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Ulfa Khaerunnisa ◽  
Arifah Rahayu ◽  
Yanyan Mulyaningsih

Katuk {Sauropus androgynus (L.) Merr} is a leafy vegetable which requires nitrogen for itsvegetative growth.  This study was aimed at assessing the agronomic performance of various accessions of katuk vegetable grown with different rates of urea fertilizer administration.  A completely randomized design with two factors was used.  The first factor was katuk accessions consisting of Sukaraja Sukabumi, Cugenang Cianjur, and Kemang Bogor.  The second factor was urea fertilizer administration in five rates, namely 0, 33.3, 66.7, 100%, and 133.3% of recommended rate (R).  Results showed that katuk plant of Sukabumi accession gave the best plant height and leaflet area.  Katuk plant of Cianjur accession had the highest values of plant height, number of buds, yield fresh weight, yield dry weight, and root length.  Katuk plant of Bogor accession was found to have the highest fresh root weight.  Urea administration of 66.7-133.3% R resulted in katuk plant with the best values of plant height, number of buds, stem diameter.  The widest leaf area was found in 100% R urea administration and the highest root weight was found in plants treated with 66.7% R urea administration.  The increase of urea fertilizer doses starting from 33.3% R in Cianjur and Bogor katuk accession increased the total harvest wet weight and dry weight, while in Sukabumi accession the significant increase in production was at a dose of 100% R and 133.3% R urea. The increase in vitamin C content was significant in the application of 133.3% R urea fertilizer.


2017 ◽  
Vol 3 (4) ◽  
pp. 157
Author(s):  
Nur Hidayaturrohmah ◽  
Sri Kasmiyati ◽  
Sucahyo . ◽  
Zane Vincēviča-Gaile

<p class="Els-Abstract-text">Plants growing in soil containing heavy metal  polutan such as chromium (Cr), lead (Pb), cadmium (Cd) and copper (Cu)  will be stunted, and  increase production of Reactive Oxygen Species (ROS). In dealing with the excess amount of ROS, plants have an enzymatic defense system, using superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). The aim of this study was to determine and analyze the <em>Sonchus oleraceus</em> (Linnaeus, 1753) plant response to heavy metals stress, seen from the growth and antioxidative defense enzymatically. Research carried out using a Completely Randomized Design (CRD) with four treatments and five replicates. The metal treatment was 10 mg · L<sup>–1</sup>. The presence of heavy metals in the growing medium significantly decreased the plant height and leaf area, so the impact is on the weight of wet and dry weight. The metal treatments of Cr, Cd, Pb and Cu increase the activity of SOD and APX enzymes but decrease the activity of the CAT enzyme. Chromium is a metal that has a significant influence on the growth and activity of SOD, APX, and CAT enzymes in <em>S. oleraceus</em>.</p><div><p class="Els-keywords"><strong>Keywords:</strong> antioxidative responses; growth; heavy metal; <em>Sonchus oleraceus; </em>toxicity.</p></div>


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