In-Vitro Antioxidant, Antibacterial and Anticancer properties of Leaf Extract Annona Muricata

2021 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Anticancer Properties

Annona muricata is one of the important herbal plant that are widely used to treat antidiabetic, anti-inflammatory, insecticidal, antimalarial, anticancer, antibacterial and antioxidant activities. In the present study, extraction procedure was carried out in few step processes method. The extract contains high percentage of steroid, alkaloid, flavonoid, phenolic and saponin. The extract was used to study the antioxidant activity; antibacterial activities and anticancer activity by standard methods. The antioxidant activity was studied by using radical scavenging DPPH, FRAP and H2O2 method and disc diffusion method. The results of antibacterial activity of the following bacteria’s such as Pseudomonas, Staphylococcus, Bacillus, E.coli shows the maximum zone of inhibition against Staphylococcus aureus which is around 1.7 cm. The anticancer activity was carried out by MTT assay using Hep-G2 as cell line and results are reported in the paper.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt

2018 ◽  
Vol 26 (2) ◽  
pp. 099
Author(s):  
Adit Widodo Santoso ◽  
Adelina Simamora ◽  
Adelina Simamora ◽  
Kris Herawan Timotius ◽  
Kris Herawan Timotius
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Traditional Use ◽  
Myristica Fragrans

<p><em>Myristica fragrans</em> Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from <em>M.</em><em> </em><em>Fragrans</em><strong>. </strong>The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from <em>M.</em><em> </em><em>fragrans</em> mace.  Both WE and EO were evaluated for their α-glucosidase inhibitory activities <em>in vitro</em> and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC<sub>50</sub> = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm.  The strongest activity was observed against<em> Staphylococcus mutans</em>. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities. </p>

scholarly journals Determination of Mineral Composition and Phenolic Content and Investigation of Antioxidant, Antidiabetic, and Antibacterial Activities of Crocus sativus L. Aqueous Stigmas Extracts

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Lemiae Zaazaa ◽  
Hanae Naceiri Mrabti ◽  
Abdelaziz Ed-Dra ◽  
Khaoula Bendahbia ◽  
Hind Hami ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Crocus Sativus ◽  
Antidiabetic Activity ◽  
Diffusion Method ◽  
Dpph Radical Scavenging ◽  
Crocus Sativus L

The aim of this study is to investigate the in vitro antioxidant, antidiabetic, and antibacterial activities of Moroccan and Italian Crocus sativus (L.) stigmas extracts. The antioxidant activity was evaluated by DPPH radical scavenging assay, and the results showed that the Moroccan extract has a powerful antioxidant activity with an IC50 of 0.32 ± 0.059 μg/mL compared to the Italian extract (IC50 of 3.14 ± 0.021 μg/mL). Additionally, the antidiabetic activity was evaluated by using alpha-amylase and alpha-glucosidase inhibition assay, and both extracts showed significant antidiabetic activity. However, the antibacterial activity was evaluated by the disc diffusion method to determine the inhibitory diameters and microplate dilutions method to determine the minimum inhibitory concentration. Our findings revealed that both Moroccan and Italian extracts were more effective against Gram-positive than Gram-negative bacteria. From this study, we can conclude that the studied extracts of C. sativus are rich in natural compounds and could have a broad application in the pharmaceutical, food, and medical fields.

scholarly journals Phytochemical Studies Antibacterial and Antioxidant Activities of Aerial Parts of Ferula lutea (Poir.) Maire.

2019 ◽  
Vol 6 (1) ◽  
pp. 17-25
Author(s):  
Ibtissem Boulacel ◽  
Brahim Harkati ◽  
Radia Ayad ◽  
Ibrahim Demirtas ◽  
Hocine Laouer ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Aerial Parts ◽  
New Compounds

Abstract The focus of this paper was to assess the phytochemical composition, the antioxidant and antibacterial activities of the crude extracts of the aerial parts of Ferula lutea. Four new compounds were isolated from this genus, and their structures were primarily established by extensive spectroscopic analysis including 1D and 2D spectroscopic techniques. Total phenolics and flavonoids containing in ethyl acetate (AcOEt) and n-butanol (n-BuOH) extracts were quantified [199.1-184.02 mg gallic acid equivalent / g of dry weight], flavonoids [70.24-65.95 mg quercetin / g dry weight]. DDPH scavenging and phosphomolybdenum methods were used to evaluate in vitro antioxidant activity. The obtained results showed a significant antioxidant activity of the two aerial parts. Correlation analysis between the DPPH values and those for TAC indicates the possibility of the two models for evaluating antioxidants power from medicinal plants. Dichloromethane (CH2Cl2), AcOEt and n-BuOH extracts were screened against two gram-negative [Escherichia coli (E.coli) and Pseudo aeruginosa (P.aer)] and two gram-positive [Staphylococcus aureus (S.aur) and Bacillus (Bacil.)] bacteria using disc diffusion method. The results indicate a good inhibition of CH2Cl2 and EtAct extracts on the growth of (Bacil. and E.coli). Furthermore n-but extract showed a significant inhibitory effect only against E.coli.

scholarly journals QUANTITATIVE ANALYSIS OF NATURAL ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITIES OF CRUCIFERAE VEGETABLES

2017 ◽  
Vol 10 (4) ◽  
pp. 470
Author(s):  
Jamuna K S ◽  
Ramesh C K ◽  
Aditya Rao Sj ◽  
Paramesha M ◽  
Riaz Mahmood
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Plant Materials ◽  
Cruciferous Vegetable ◽  
The Relationship ◽  
In Vitro Antioxidant Activity

Objective: The aim of this research program is to evaluate the antioxidant potentialities comprehensively utilizing commonly available cruciferous vegetables of India, viz., cabbage, cauliflower, kohlrabi, and radish.Methods: The plant materials were procured and processed for cold extraction procedure using 70% ethanol. The extracts were primarily assessedfor their phytoconstituents and further for their in vitro antioxidant activity using various qualitative and quantitative estimations. The results of quantitative estimations were expressed in terms of equivalence with respective standards, and the antioxidant potentiality of various scavenging and chelating activities were expressed in terms of 50% effective concentration. Further, correlation studies were made between quantitative and qualitative assays to study the relationship between the effects of different phytoconstituents groups.Results: In this study, the results revealed that all the four cruciferous vegetable extracts possess potential antioxidant activities. Among the extractsunder study kohlrabi has recorded superior antioxidant potential than others. It is clear from the study that the tested cruciferous vegetablesmanifested differential expression of antioxidant capacity due to their phytoconstituents.Conclusion: From the results, it can be concluded that the Cruciferae vegetables have potent antioxidant activity contributing to the use for healthbenefits in addition to their nutritive role as vegetable.Keywords: Cruciferae vegetables, Phytochemicals, In vitro antioxidant activity, Correlation.

scholarly journals In Vitro Antibacterial and Antioxidant Activities of Roasted and Green Coffee Beans Originating from Different Regions of Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Tsedale Tasew ◽  
Yalemtsehay Mekonnen ◽  
Tegenu Gelana ◽  
Mesfin Redi-Abshiro ◽  
Bhagwan Singh Chandravanshi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Addis Ababa ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Coffee Bean ◽  
Green Coffee ◽  
Bacterial Strains ◽  
Coffee Shop

Coffee is among the most traded commodities and consumed beverages worldwide primarily for its stimulating effects. Moreover, coffee is known to contain various bioactive compounds with significant health benefits including antibacterial and antioxidant activities. However, Ethiopia as the origin of coffee and producer and exporter of varieties of Coffea arabica has made little study on the health aspects of this beverage. The aim of this study was to examine the antibacterial and antioxidant activities and content of coffee samples from different localities of Yorgacheffe and Jimma; and roasted, ground, and packed samples purchased from a coffee shop in Addis Ababa. Medium-roasted-boiled and lyophilized coffee extracts were tested on eight gram-negative and gram-positive bacterial strains. The agar-well diffusion method was used to test the extracts determining the minimum inhibitory and bactericidal concentrations. For coffee antioxidant activity and content, light-roasted (both field and shop samples) and green coffee bean extracts were tested using the free radical 2.2-diphenyl-l-pict1hydrazyl (DPPH) IC50 percent inhibition protocol. The samples showed strong antibacterial and antioxidant activity and substantial antioxidant content. Significant variation was noted in the antibacterial activities of the different coffee samples. Moreover, the growth-inhibitory strength of each coffee sample was variable for different test bacteria. A coffee sample with the best antibacterial activity had also the highest antioxidant activity/content. The shop coffee had the lowest bioactivity. The observed variations in the antibacterial and antioxidant activities among the samples probably indicate the diversity of the Ethiopian coffee related, among other factors, to the coffee plant genetics and agroecology.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

In Vitro Antimicrobial and Antioxidant Activities of Organic and Aqueous Extracts of Bacillus clausii KP10

2019 ◽  
Vol 41 (1) ◽  
pp. 161-161
Author(s):  
Farzana Nighat and Zahid Mushtaq Farzana Nighat and Zahid Mushtaq
Keyword(s):  
Hot Springs ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Current Case ◽  
Bacillus Clausii

Humans and plants are experiencing different kinds of diseases. Many of these diseases are mainly caused by microorganisms like fungi, bacteria and viruses that adversely affect their hosts. Bioactive compounds produced by bacteria can be used to treat the infections caused by these microorganisms. Free radicals that can cause serious damage to the cell can be repressed by antioxidants that are produced by bacteria as secondary compounds. The current case study was therefore planned to investigate the antimicrobial and antioxidant potentials of the organic and aqueous extracts of Bacillus clausii KP10 isolated recently by our group from hot springs of Tatta Pani, Poonch, Azad Kashmir. The antimicrobial activities were assessed using well diffusion method against two Gram positive bacteria; B. subtilis, and S. aureus, and two Gram negative bacteria; E. coli and P. multocida and three fungal strains; A. niger, A. parasiticus and A. ustus. Antioxidant activities of the extracts were done by DPPH free radical scavenging assay. In our experiments the maximum zones of inhibition were shown by ethanolic extract against P. multocida (21 mm), S. aureus (13 mm) and methanol soluble fraction showed zone of inhibition against A. ustus (21 mm) as compared to the standard ciprofloxacin for bacteria and as antifungal agents with terbinafine. In our studies n-hexane fraction of B. clausii KP10 showed maximum 88.67% antioxidant activity. Finally, results were analyzed statistically through analysis of variance (ANOVA). Hence it is proved that the bioactive fractions obtained from B. clausii KP10 are good source of antimicrobials and antioxidants and they can be used as medicines in future for the respective treatments.

scholarly journals Selenylation Modification of Atractylodes macrocephala Polysaccharide and Evaluation of Antioxidant Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ranran Hou ◽  
Qiu Li ◽  
Jie Liu ◽  
Yuanliang Hu
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Effect ◽  
Ftir Spectrometry ◽  
Mda Content ◽  
Selenylation Modification ◽  
Atractylodes Macrocephala

The Atractylodes macrocephala polysaccharide (AMP) was extracted by water extracting-alcohol precipitating method and further purified by DEAE column. After that, the polysaccharides were modified by nitric acid-sodium selenite method, and nine kinds of selenizing AMPs (sAMPs) were obtained, namely, from sAMP1 to sAMP9. AMP and sAMP were characterized using FTIR spectrometry. Then their antioxidant activities in vitro were measured by free radical-scavenging test. Among these, sAMP6 presented the strongest antioxidant effect. For the test in vivo, the chickens at day 14 vaccinated with ND vaccine were repeatedly vaccinated at day 28. The chickens in sAMP and AMP were injected respectively with 1 mg of sAMP6 and AMP and, in vaccination control (VC) and BC groups, injected with equal volume of normal saline. Respectively, after the first vaccine, on days 7, 14, 21, and 28, the serum GSH-Px and SOD activities and MDA content were determined. The results suggested that sAMP6 could significantly promote GSH-Px and SOD activities and decrease MDA content. All these results indicated that selenylation modification could significantly enhance the antioxidant activity of AMP.

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