scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENAPISAN FITOKIMIA DARI EKSTRAK DAUN PAKOASI DAN KLUWIH SEBAGAI SUMBER ANTIOKSIDAN ALAMI

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Nurlaila Agustikawati ◽  
Yayuk Andayani ◽  
Dedy Suhendra
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Leaf Analysis ◽  
Activity Test ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Bean Leaves

This study aims to determine the antioxidant activity and secondary metabolism of pakoasi leaf extract and kluwih leaf. Analysis using DDPH method (1,1-diphenyl-2-picrylhydrazyl). Result of antioxidant activity test in. Concentration Barriers (IC50). IC50 values for leaf extract of pakoasi and kluwih leaves were 89.659 μg / mL and 54.719 μg / mL greater than IC50 vitamin C of 46.74 μg / mL. Qualitative analysis of antioxidant compounds using TLC technique showed seven chromatogram profiles for pakoasi leaf extract and four chromatogram profiles for kluwih leaf extract. In addition, phytochemical analysis of leaf extract of pakoasi and kluwih leaves showed the presence of saponin compounds, tannins, phenols, flavonoids, steroids and triterpenoids. Based on the results of this analysis concluded that leaf extract of pakoasi and kluwih leaf as natural source of antioxidant which can be seen with IC50 value in the sample is in strong category and supported from phytolyte analysis result which according to phenol and flavonoid which act as antioxidant.Keywords: Antioxidant activity, phytochemical analysis ethanol extract, green bean leaves, kluwih leaves, leaves of pakoasi

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antioksidan dari Daun Nasi (Phrynium capitatum) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 11 ◽  
Author(s):  
Dwi Putra Wijaya ◽  
Jessy E. Paendong ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dpph Solution

Telah dilakukan penelitian tentang skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Skrining fitokimia meliputi pengujian alkaloid, flavonoid, saponin, steroid, triterpenoid dan fenolik. Uji aktivitas antioksidan dilakukan dengan penambahan 2 mL larutan DPPH dengan 0,5 mL larutan ekstrak dengan konsentrasi masing-masing 1000, 750, 500 dan 250 µg/mL. Data yang diproleh kemudian digunakan untuk menghitung nilai IC50 dari ekstrak etanol daun nasi. Hasil pengujian menunjukan bahwa ekstrak etanol daun nasi mengandung senyawa alkaloid, flavonoid, saponin, steroid dan fenolik. Ekstrak etanol daun nasi memiliki aktivitas antioksidan trrtinggi pada konsentrasi 1000 µg/mL sebesar 65,77 % dan semakin menurun aktivitasnya dengan berkurangnya konsentrasi ekstrak, konsentrasi 750, 500 dan 250 µg/mL masing-masing sebesar 53,96 %, 43,8 % dan 19,58 % dengan nilai IC50 pada konsentrasi 701,78 µg/mL.A research on phytochemicals screening and antioxidant activity test of daun nasi (Phrynium capitatum) using DPPH (1,1-diphenyl-2-picrylhydrazyl) method had been conducted. Test for alkaloids, flavonoids, saponins, steroids, triterpenoids and phenolic were included in phytochemical screening. Antioxidant activity was tested through the addition of 2 mL DPPH solution into 0.5 mL of the extract having a concentration of 1000, 750, 500 and 250 µg/mL respectively. The data obtained was used to calculate the IC50 value for the ethanol extract of daun nasi. The results showed that the ethanol extract of daun nasi contains alkaloids, flavonoids, saponins, steroids and phenolic. Ethanol extract of daun nasi has the highest antioxidant activity of 65.77% at concentration of 1000 µg/mL. Its activity decreased with decreasing concentration of the extract, which were 53.96 %, 43.8 % and 19.58 % at concentration of 750, 500 and 250 µg/mL respectively. IC50 values was found at a concentration of 701.78 µg/mL.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

scholarly journals Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

2021 ◽  
Vol 5 (4) ◽  
pp. 365-368
Author(s):  
Muthmainah Tuldjanah ◽  
Niluh Puspita Dewi ◽  
Dian Rahmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
Morinda Citrifolia ◽  
Absorption Method ◽  
Immersion Method ◽  
Activity Test ◽  
Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

scholarly journals KAJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN TEMBELEKAN (Lantana camara L) BERDASARKAN TINGKAT KEPOLARAN PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (3) ◽  
Author(s):  
Oktavian Mangela ◽  
Ahmad Ridhay ◽  
Musafira Musafira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Antioxidant Compounds ◽  
Ic50 Value

The investigation about the antioxidant activity of tembelekan (Lantana camara L) leaf extract based on level polar solvent . Has been done the aim of this study was to determine the IC50 value and to know the content of antioxidant compounds from tembelekan leaf extract based on the level of polar solvent the extraction of tembelekan leaf was done with maceration method . The result showed that ethyl acetate extract of tembelekan leaf has the highest antioxidant activity with IC50 value was 71,70 ppm. Keywords: Tembelekan ( Lantana Camara L ) , IC50 , Antioxidant , DPPH

scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

AKTIVITAS ANTIOKSIDAN EKSTRAK UMBI SUWEG (Amorphophallus paeoniifolius) DARI BERBAGAI TINGKAT POLARITAS PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Deddy Firman ◽  
Nurhaeni Nurhaeni ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Tuber Type

ABSTRACT Has done research about antioxidant activity of suweg tuber (Amorphophallus campamulatus) based on the level of polar solvent. This research begins with in phased maceration extraction using n-hexane, ethyl acetate dan ethanol. Next step was antioxidant activity test with tyocyanate to n-hexane extract and DPPH methods to ethanol extract, ethyl acetate and vitamin C. The result showed antioxidant activity in n-hexane extract i.e. 14,96 %, end 77,13 % BHT while in ethyl acetate, ethanol and vitamin C reached IC50 value are 458,102 ppm, 223,268 ppm dan 26,76 ppm. Keywords: Suweg tuber, type of solvent, maceration, antioxidant

scholarly journals UJI FITOKIMIA DAN PENENTUAN Inhibition Concentration 50% PADA BEBERAPA TUMBUHAN OBAT DI PULAU TIDORE

2014 ◽  
Vol 14 (2) ◽  
pp. 95
Author(s):  
Wiwin Abdullah ◽  
Max Revolta J. Runtuwene ◽  
Vanda Selvana Kamu
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Seed Extract ◽  
Test Results ◽  
Active Compounds ◽  
Inhibition Concentration ◽  
Dpph Method ◽  
Ic50 Values

ABSTRAK Penelitian ini dilakukan untuk mengetahui senyawa aktif yang terkandung melalui pengujian fitokimia dan aktivitas antioksidan pada tumbuhan obat di pulau Tidore. Uji aktivitas antioksidan menggunakan metode DPPH dilanjutkan dengan perhitungan Inhibition Concentration 50% (IC50). Hasil yang diperoleh adalah biji buah mojoi terkandung senyawa alkaloid dan saponin, buah coro terkandung alkaloid, flavonoid, dan saponin, pada daun ofo terkandung  alkaloid, tanin, flavonoid, steroid, dan saponin dan pada rimpang kuso mafola terkandung alkaloid, tanin, flavonoid, steroid, dan saponin. Nilai IC50 sebagai berikut biji rimpang kuso mafola 37,30 ppm, buah coro 250,17 ppm, daun ofo 976,10 ppm  dan buah mojoi 1001, 07 ppm. Kata kunci :Tumbuhan  obat, DPPH, antioksidan dan uji fitokimia. PHYTOCHEMICALS TEST AND DETERMINATION Inhibition Concentration 50% ON SOME MEDICINAL PLANTS IN THE TIDORE ISLAND ABSTRACT This study was conducted to determine the active compounds contained in an assessment of phytochemical and antioxidant activity in the medicinal plants of Tidore island. The test antioxidant activity was used DPPH method. In the test results to the phytochemical , that mojoi fruit seeds contained alkaloids and saponins, fruit coro (alkaloids, flavonoids, and saponins), ofo leaves (alkaloids, tannins, flavonoids, steroids, and saponins) and ethanol extract of rhizome kusomafola (alkaloids, tannins, flavonoids, steroids, and saponins. In  calculation of IC50 values ​​for rhizome kusomafola 37.30 ppm, 250.17 ppm coro fruit, 976.10 ppm ofo leaf extract, and fruit seed extract mojoi 1001.07 ppm. Keywords: Medicinal plants, DPPH, antioxidant and phytochemical test.

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