scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used as well as UV-Vis Spectrophotometry was used to measure vitamin C as a positive control measurement. The samples mixed with ethanol in various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619 mg/L, 16.575 mg/L, 14.729 mg/L, and 16.782 mg/L, respectively. Vitamin C as the positive control has IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 349
Author(s):  
Eklesia Pogaga ◽  
Paulina V. Y. Yamlean ◽  
Julianri S. Lebang
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Traditional Medicine ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Mulberry Leaves ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan  banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm  dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat  dengan  nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (3) ◽  
pp. 107
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is to determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used and UV-Vis spectrophotometry was used to measure Vitamin C as a positive control measurement. The samples mixed with ethanol in a various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619, 16.575, 14.729, and 16.782 mg/L, respectively. Vitamin C as positive control has an IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals The Measurement of Antioxidant Activity of Velvet Beans (Mucuna pruriens) and Velvet Beans (Mucuna pruriens) in Coffee Preparations

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 117-122
Author(s):  
Indah Tri Susilowati ◽  
Purwati Purwati
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Mucuna Pruriens ◽  
Methanol Extracts ◽  
Activity Test ◽  
Antioxidant Content ◽  
Ic50 Values

Antioxidant activity tests of methanol extracts of velvet beans (Mucuna pruriens) and velvet beans (Mucuna pruriens) in coffee preparations have been previously carried out by several researchers. This present study aims to examine the phytochemical and antioxidant activities of methanol extracts of Mucuna pruriens and Mucuna pruriens in coffee preparations. The methanol extract was tested for the phytochemical and antioxidant activities by applying DPPH (1,1-Dhiphenyl-2-picrylhydrazil) method. The results of the phytochemical test showed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations contained secondary metabolites of flavonoids, alkaloids, saponins, terpenoids, and tannins. The outcomes of the antioxidant activity test revealed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations had antioxidant activities, as indicated by the IC50 values of 42.09 ppm and 37.23 ppm. It was also revealed that the antioxidant content of Mucuna pruriens in coffee preparations was stronger than the content in the velvet beans alone.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Firdaus Firdaus ◽  
Jalifah Latip
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Vitamin ◽  
Activity Assay ◽  
Immersion Method ◽  
Ic50 Value ◽  
Measured Absorption ◽  
Hexane Extracts

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of  T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL. 

scholarly journals POTENSI EKSTRAK ETANOL DAUN ANDONG MERAH (Cordyline fruticosa (L.) A. Cheval) SEBAGAI ANTIOKSIDAN PENANGKAL RADIKAL DPPH

2021 ◽  
Vol 4 (1) ◽  
pp. 24
Author(s):  
Yuri Pratiwi Utami
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Traditional Medicinal Plants ◽  
Ic50 Value ◽  
Cordyline Fruticosa

AbstractAndong leaf is one of the traditional medicinal plants that is proven to have various properties including as a (natural) medicinal ingredient. Andong plants contain several chemical compounds including saponins, tannins, flavonoids, polyphenols, steroids. The purpose of this study was to determine the potential of red andong leaf extract (Cordyline fruticosa (L.) A. Cheval) as a radical scavenging antioxidant for DPPH. In this study red andong leaves (Cordyline fruticosa (L.) A. Cheval) were extracted using maceration method with 70% ethanol as solvent. The results showed that anthocyanins were cyanidin. Antioxidant activity was measured by reducing DPPH free radicals with UV-Vis spectrophotometry at a wavelength of 517 nm. The results showed that red andong red leaf extract had strong antioxidant potential with an IC50 value of 64.5197 g/mL against free radicals DPPH and vitamin C as a comparison showed a very strong antioxidant potential with an IC50 value of 2.12 g/mL.Keywords : Andong leaf, antioxidant, DPPH AbstrakDaun andong merupakan salah satu tanaman obat tradisional yang terbukti memiliki berbagai khasiat diantaranya sebagai bahan obat (alami). Tanaman andong mengandung beberapa senyawa kimia diantaranya yaitu saponin, tanin, flavonoid, polifenol, Steroida. Tujuan dari penelitian ini adalah untuk mengetahui potensi  ekstrak daun andong merah (Cordyline fruticosa (L.) A. Cheval) sebagai antioksidan penangkal  radikal DPPH. Pada penelitian ini daun andong merah (Cordyline fruticosa (L.) A. Cheval) diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 70%. Hasil penelitian menunjukkan  antosianin jenis  sianidin. Aktivitas antioksidan diukur melalui peredaman radikal bebas DPPH dengan spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil Penelitian menunjukkan ekstrak daun andong merah   memiliki potensi  antioksidan  kuat dengan nilai IC50 64.5197 µg/mL terhadap radikal bebas  DPPH dan vitamin C sebagai pembanding menunjukkan potensi antioksidan sangat kuat dengan nilai  IC50 2.12 µg/mL.Kata Kunci : Daun andong merah, antioksidan, DPPH

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

scholarly journals Fruit Pomological, Phytochemical Characteristic and Mineral Content of Rosehip Genotypes

2020 ◽  
pp. 18-23
Author(s):  
Nihal Acarsoy Bilgin ◽  
◽  
Adalet Mısırlı ◽  
Fatih Şen ◽  
Bilge Türk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Vitamin C ◽  
Mineral Content ◽  
Antioxidant Activities ◽  
Total Phenol ◽  
High Demand ◽  
Nutrition And Health ◽  
Mineral Contents

Rosehips, which are commonly grown in nature in shrub form, are always popular due to their importance in terms of nutrition and health. Because fruits are rich in vitamin C, total phenol and mineral contents and antioxidant activities. Nowadays, there is high demand for natural products. Rosehip grows spontaneously in Turkey. In this study, 9 individuals were examined. Consequently, significant differences were stated according to genotypes for pomological and phytochemical characteristics. Vitamin C, phenol and antioxidant activity were found to affect P and Zn nutrients. Additionally, it was detected that P, K, Ca, Mg, Na Fe, Cu, Zn and Mn contents ranged in 0.09-0.17%, 1.35-1.86%, 1.05-1.44%, 0.43-069%, 50.00-100.00 ppm 28.50-48.30 ppm, 3.30-7.00 ppm, 8.06-12.56 ppm and 50.85-85.35 ppm, respectively. In this context, one of the genotypes has been found promising due to its content.

scholarly journals Kandungan Fitokimia dan Bioaktivitas Ekstrak Metanol Biji Palem Putri (Veitchia merillii)

2016 ◽  
Vol 2 (1) ◽  
pp. 63-70
Author(s):  
Adawiah Adawiah
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Radical Scavenging ◽  
Positive Control ◽  
Antidiabetic Activity ◽  
Phytochemical Content ◽  
Sample Extract ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Alpha Glucosidase

Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of  0.82 ppm, the level of toxicity is very toxic with LC50 values ​​of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values ​​of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control.  Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of  0.82 ppm, the level of toxicity is very toxic with LC50 values ​​of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values ​​of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control.   Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity DOI: http://dx.doi.org/10.15408/jkv.v2i1.3076

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