scholarly journals Ground Applications of Vectobac® WDG with A1 Super-Duty Mist Sprayer® and Micronair® AU5000 Atomizer for Suppression of Aedes aegypti Populations in the Florida Keys

2021 ◽  
Vol 37 (4) ◽  
pp. 271-279
Author(s):  
Heidi L. Murray ◽  
Catherine A. Pruszynski ◽  
Lawrence J. Hribar
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Florida Keys ◽  
Application Rate ◽  
Residential Area ◽  
Distilled Water ◽  
Bacillus Thuringiensis Israelensis ◽  
Key West ◽  
F1 Generation

ABSTRACT Since 2011, the Florida Keys Mosquito Control District (FKMCD) has used the WALS® application strategy with VectoBac® WDG containing Bacillus thuringiensis israelensis via helicopter in Key West for the control of Aedes aegypti larval populations. In 2018, FKMCD conducted a study to determine the effectiveness of using a trailer-mounted A1 Super Duty Mist Sprayer® (A1 Mist Sprayers) with a Micronair® AU5000 (Micron Group) atomizer to apply VectoBac WDG by ground at the rate of 0.5 lb/acre (0.56 kg/ha). Bioassay cups were placed in a residential area encompassing open, moderate, and heavy cover scenarios between 0 and 300 ft (0–91.44 m) perpendicular to the spray line. An application rate of 0.5 lbs/acre (0.56 kg/ha) was used. Bioassay cups were collected after application and returned to the laboratory where 100 ml of distilled water and 10 F1 generation Ae. aegypti larvae were added. Laval mortality was monitored at 2, 4, and 24 h. Three separate runs were completed during the summer of 2018. Average larval mortality at 24 h was >90%. The field trial demonstrated sufficient efficacy to introduce this method of larviciding into operational use.

scholarly journals Application Efficacy of Vectobac WDG Against Larval Aedes aegypti Using Thermal Fog Technology

2018 ◽  
Vol 34 (1) ◽  
pp. 75-77
Author(s):  
J. A. Knapp ◽  
C. M. Waits ◽  
A. K. C. Briley ◽  
J. E. Cilek ◽  
A. G. Richardson ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Open Field ◽  
Forest Canopy ◽  
Larval Mortality ◽  
Tropical Environment ◽  
Bacillus Thuringiensis Israelensis ◽  
Key West ◽  
Operational Efficacy

ABSTRACT The effectiveness of thermal fog application of Bacillus thuringiensis israelensis (Vectobac® WDG) against larval Aedes aegypti was evaluated in open and forest canopied environments in northeastern Florida, using a handheld IGEBA® TF34 thermal fogger (with water conversion kit). The product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. After application, late-2nd to early-3rd instars of Ae. aegypti were added to each cup. Complete larval mortality occurred 48 h after treatment, 8 m away from the spray line in the open field while 63.4% larval mortality was achieved at this same distance in the forest canopy at 72 h posttreatment. Operational efficacy was then evaluated in an urban tropical environment in Key West, FL. In this study, empty plastic cups were placed in cryptic areas of a commercial lobster and crab trap yard. The 0.1-ha area was treated with Vectobac WDG at the maximum label rate using the same equipment. Aedes aegypti larval mortality from treated cups was >99% at 24 h posttreatment, whereas complete mortality was achieved at 48 h after application.

scholarly journals Larvicidal Effectiveness of Azolla pinnata against Aedes aegypti (Diptera: Culicidae) with Its Effects on Larval Morphology and Visualization of Behavioural Response

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Nor Shaida Husna Zulkrnin ◽  
Nurul Nadiah Rozhan ◽  
Nur Amanina Zulkfili ◽  
Nik Raihan Nik Yusoff ◽  
Mohd Sukhairi Mat Rasat ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Control Program ◽  
Test Method ◽  
World Health ◽  
Azolla Pinnata ◽  
Agricultural Field ◽  
Aquatic Fern ◽  
Significant Difference

Dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. Aedes aegypti (L.) (Diptera: Culicidae) is a primary vector for dengue viral infections for humans. Current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. The extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and development of insecticide resistance on mosquitoes. Azolla pinnata is an aquatic fern and predominantly used as feed in poultry industry and as fertilizer in agricultural field for enhancing the fertility of rice paddy soil. The present study was conducted to explore the larvicidal efficacy of A. pinnata using fresh and powdered form against late third-stage larvae (6 days, 5 mm in larvae body length) of Ae. aegypti (L.) (Diptera: Culicidae). The larvicidal bioassays were performed using World Health Organization standard larval susceptibility test method for different concentration for powdered and fresh A. pinnata. Powdered A. pinnata concentration used during larvicidal bioassay ranges from 500ppm to 2000ppm; meanwhile, fresh A. pinnata ranges from 500ppm to 9,000,000 ppm. The highest mortality was at 1853 ppm for powdered A. pinnata compared with fresh A. pinnata at 2,521,535 ppm, while the LC50 for both powdered and fresh A. pinnata recorded at 1262 ppm and 1853 ppm, respectively. Finally, the analysis of variance (ANOVA) showed significant difference on Ae. aegypti larval mortality (F=30.439, df=1, p≤0.001) and concentration (F=20.002, df=1, p≤0.001) compared to powdered and fresh A. pinnata at 24-hour bioassay test. In conclusion, the powdered A. pinnata serves as a good larvicidal agent against Ae. aegypti (L.) (Diptera: Culicidae) and this study provided information on the lethal concentration that may have potential for a more eco-friendly Aedes mosquito control program.

Human Blood Feeding by Aedes aegypti (Diptera: Culicidae) in the Florida Keys and a Review of the Literature

2020 ◽  
Vol 57 (5) ◽  
pp. 1640-1647
Author(s):  
Catherine A Pruszynski ◽  
Tanise Stenn ◽  
Carolina Acevedo ◽  
Andrea L Leal ◽  
Nathan D Burkett-Cadena
Keyword(s):  
United States ◽  
Aedes Aegypti ◽  
Human Blood ◽  
Florida Keys ◽  
Blood Feeding ◽  
The United States ◽  
High Rate ◽  
Surveillance Program ◽  
Key West ◽  
Local Transmission

Abstract Aedes aegypti L. is considered to have a proclivity for feeding on human blood even when other hosts are available. However, few studies have demonstrated host use by this mosquito in the continental United States, where local transmission of dengue, Zika, and chikungunya viruses has been recently documented. This study investigated the bloodmeal sources of female Ae. aegypti in the subtropical city of Key West and the surrounding county in Florida with the goal of identifying preferred hosts. Blood-engorged Ae. aegypti mosquitoes were collected from BG Sentinel traps used as part of a routine surveillance program in the Florida Keys (Monroe County, Florida). Bloodmeal samples were analyzed using PCR assays, sequencing, and comparison with reference sequences in GenBank. Aedes aegypti females from Key West fed predominantly on humans (79.6%) and did not differ statistically from females collected from the rest of the Florida Keys (69.5%). Culex quinquefasciatus Say (Diptera: Culicidae), considered a host generalist, was collected and analyzed from the same sites for comparative purposes. Females of Cx. quinquefasciatus fed predominantly (70.7%) on birds and nonhuman mammals in the Florida Keys, corroborating the validity of molecular assay breadth and demonstrating that given the same group of available hosts Ae. aegypti selects humans. Our results indicated that Ae. aegypti has a high rate of human-biting in a subtropical area within the United States, supporting its role in recent local transmission of dengue and other viruses.

scholarly journals How long is long enough? Decreasing effects in Aedes aegypti larval mortality by plant extracts over time

2021 ◽  
Vol 56 (2) ◽  
pp. 338-345
Author(s):  
Gilberto Dinis Cozzer ◽  
Renan de Souza Rezende ◽  
Junir Antônio Lutinski ◽  
Walter Antônio Roman ◽  
Maria Assunta Busato ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Plant Extracts ◽  
Mosquito Control ◽  
Population Control ◽  
Larval Mortality ◽  
Residual Effect ◽  
Ilex Paraguariensis ◽  
Short Time Scale ◽  
Human Intoxication ◽  
Short Time

Aedes aegypti has overcome all kinds of mosquito control attempts over the last century. Strategies for population control resorts to the use of synthetic insecticides, which can lead to problems like human intoxication and environmental contamination. The effects of Bacillus thuringiensis var. israelensis (Bti), Ilex paraguariensis (yerba mate), and Ilex theezans (caúna herb) extracts against A. aegypti larvae were evaluated. The bioassays were conducted under controlled laboratory conditions of temperature (27 ± 3°C) and photoperiod (12 h). Hydroalcoholic extract of the leaves of I. theezans displayed better residual effect compared to the aqueous extract of I. paraguariensis fruits. The strongest residual effect of I. theezans was probably due to the presence of certain chemicals in its leaves, such as coumarins, hemolytic saponins, and cyanogenic glucosides, which were absent in I. paraguariensis. The results herein contributed to the prospection of natural insecticides and opened the possibility for subsequent studies on the use of plant extracts in field situations in a short-time scale.

scholarly journals From Surveillance To Control: Evaluation of A Larvicide Intervention Against Aedes aegypti In Brownsville, Texas

2019 ◽  
Vol 35 (3) ◽  
pp. 233-237
Author(s):  
Selene M. Garcia-Luna ◽  
Luis Fernando Chaves ◽  
José G. Juarez ◽  
Bethany G. Bolling ◽  
Arturo Rodriguez ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
South Texas ◽  
Bacillus Thuringiensis Israelensis ◽  
Department Of State ◽  
Texas Department ◽  
Control Evaluation ◽  
The City ◽  
Contingency Contract ◽  
Local Transmission

ABSTRACT South Texas is recognized as a potential area for the emergence and re-emergence of mosquito-borne diseases due to recent circulation of Zika, chikungunya, and dengue viruses. During 2017, high Aedes aegypti abundance found in the city of Brownsville, TX, in combination with the previous year's local transmission of Zika virus, triggered the activation of the Texas Department of State Health Services Emergency Mosquito Control Contingency Contract. A contract with the Clarke Environmental and Mosquito Control was a response to control Ae. aegypti, using a ground-based wide-area larvicide spray (WALS™) containing Bacillus thuringiensis israelensis. The WALS application was evaluated through a field-based bioassay and by comparing surveillance data pre- and post-WALS application. The WALS application bioassay demonstrated that the larvicide was effective up to 60 m into the target properties. Additionally, the number of Ae. aegypti captured in traps decreased in the WALS intervention areas compared with the untreated control areas, with an estimated 29% control.

scholarly journals How long is long enough? Decreasing on plant extract lethal effects, over time, affecting Aedes aegypti larval mortality

2019 ◽  
Author(s):  
Gilberto Dinis Cozzer ◽  
Renan de Souza Rezende ◽  
Junir Antônio Lutinski ◽  
Walter Antônio Roman Junior ◽  
Maria Assunta Busato ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Population Control ◽  
Larval Mortality ◽  
Food Resource ◽  
Residual Effect ◽  
Alternative Methods ◽  
Ilex Paraguariensis ◽  
Short Time Scale ◽  
Over Time

AbstractThe mosquito Aedes aegypti has overcome all kinds of human being mosquito control attempts over the last century. Strategies for vector population control resorts to the use of synthetic insecticides, which can lead to problems of intoxication in humans and environmental contamination. We evaluated the effects of Bacillus thuringiensis var. israelensis (Bti), Ilex paraguariensis (mate-herb) and Ilex theezans (caúna-herb) extracts against A. aegypti larvae mortality. The bioassays were conducted under controlled laboratory conditions of temperature (27±3°C) and photoperiod (12h). Hydroalcoholic I. theezans leaves extract displayed better residual effect compared to I. paraguariensis fruit aqueous extract. Variation in larval mortality was also observed in the exposure periods (low after a few weeks). Low mortality after a few weeks may mean increased the food for mosquito in a oppose effect over time. The residual effect of Bti was observed during the 56 days of the study duration (100% of mortality). The strongest residual effect of I. theezans was probably due to the presence of chemical on its leaves, such coumarins, hemolytic saponins and cyanogenic glucosides, absent in I. paraguariensis. On the other hand, alternative methods to vector control present risks in a long term scale by reversal of larvicide effect into food resource. Our results contributed to the prospection of natural insecticides and open the possibility for subsequent studies of the use of plant extracts in field situations in a short time scale.

scholarly journals Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti (The Effectiveness of Citronella Extract (Cymbopogon nardus) as Larvaside of Aedes aegypti)

2019 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Makkiah Makkiah ◽  
Cristina L Salaki ◽  
Berty Assa
Keyword(s):  
Experimental Study ◽  
Regression Analysis ◽  
Aedes Aegypti ◽  
Crude Extract ◽  
Larval Stage ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Probit Regression ◽  
Extract Concentration ◽  
Cymbopogon Nardus

Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti (The Effectiveness of Citronella Extract (Cymbopogon nardus) as Larvaside of Aedes aegypti) Makkiah*), Cristina L Salaki **), Berty AssaProgram Studi Entomologi Pascasarjana Universitas Sam RatulangiManado, 95155*Email: [email protected],**Email korespondensi: [email protected]  (Article History: Received 20-10-2019; Revised 27-11-2019; Accepted 30-12-2019) Abstrak Pengendalian nyamuk sebagai vektor utama penularan penyakit DBD hanya dapat dilakukkan dengan cara memutuskan rantai penularannya melalui pengendalian nyamuk. Pengendalian nyamuk dapat dilakukan pada tahap larva dengan menggunakan biolarvasida yang terbuat dari bahan alami dan salah satunya adalah dengan menggunakan ekstrak kasar dari tanaman serai wangi (Cymbopogon nardus). Penelitian ini bertujuan untuk menguji efektivitas ekstrak serai wangi sebagai larvasida larva Aedes aegypti instar III dan IV. Penelitian ini adalah penelitian eksperimental menggunakan rancangan acak lengkap. Larva yang digunakan adalah larva instar III dan IV, dengan  perlakuan konsentrasi ekstrak serai wangi 20%, 30%, 40%, dan 50% dalam 250 ml air. Pada penelitian ini terdapat kontrol yaitu aquades yang tidak ditambahkan larutan apapun. Pengamatan kematian larva dilakukan pada jam ke 1, 3, 6, 12, 24, dan jam ke 48 dengan replikasi sebanyak 4 kali. Data presentase mortalitas larva digunakan untuk menghitung nilai LC50 dan LT50 dengan menggunakan analisisi Probit. Hasil penelitian menunjukkan ekstrak serai wangi efektif dalam mematikan 50% dari populasi  larva uji  dengan  nilai LC50 pada konsentrasi 36,48%serta waktu yang di butuhkan untuk mematikan  50% populasi larva uji adalah 10,45 jam. Kata kunci : Ekstrak, Cymbopogon nardus, larvasida, Aedes aegypti Abstract Control of mosquitoes as the main vector of transmission of DHF can only be done by breaking the chain of transmission through mosquito control. Mosquito control can be carried out at the larval stage by using biolarvasides made from natural materials, one of which is by using a crude extract from the citronella plant (Cymbopogon nardus). This research is to find out the effectiveness of citronella extract as larvae of Aedes aegypti larvae instar III and IV. This research is a pure experimental study using a complete random design. The larvae used were instar larvae III and IV, with the treatment of citronella extract concentration of 20%, 30%, 40%, and 50% in 250 ml of water. In this study, there was a control i.e. aquades which did not add any solution. Observation of larvae death was done at 1 hour, 2 hours. 4 hours, 12 hours, 24 hours and 28 hours with replication 4 times. Data on larval mortality were used to calculate LC50 and LT 50 values using Probit regression analysis. The results showed that citronella extract was effective in killing 50% of the population of test larvae with LC50 values at a concentration of 36.48% and the time needed to kill 50% of the population of test larvae was 10,450 hours.Key words:  Extract, Cymbopogon nardus, larvasida, Aedes aegypti

scholarly journals Effectiveness of adulticide and larvicide in controlling high densities of Aedes aegypti in urban environments

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0246046
Author(s):  
André B. B. Wilke ◽  
Chalmers Vasquez ◽  
Augusto Carvajal ◽  
Monica Ramirez ◽  
Gabriel Cardenas ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Relative Abundance ◽  
Urban Areas ◽  
Mosquito Control ◽  
Urban Environments ◽  
Baseline Survey ◽  
Dade County ◽  
Bacillus Thuringiensis Israelensis ◽  
Breeding Habitats ◽  
And Control

Current management and control of Aedes aegypti populations in urban areas are based on the spraying of insecticides. Here, we evaluated the effectiveness of spraying larvicide (Bacillus thuringiensis israelensis) using a truck-mounted Buffalo Turbine and adulticide (Deltamethrin) using a Grizzly ULV Sprayer in an urban area with high densities of Ae. aegypti and many cryptic and difficult to reach aquatic breeding habitats. Experiments were conducted in a tire shop located in Miami-Dade County, Florida with approximately 100,000 used airplane tires. Insecticide interventions were performed after a baseline survey consisting of 3 weeks of collections, followed by two insecticide interventions: (i) application of the adulticide followed by the application of larvicide on the subsequent week; and (ii) application of both adulticide and larvicide on two consecutive weeks. The first insecticide intervention resulted in a non-significant decrease in the relative abundance of Ae. aegypti. On the other hand, the second insecticide intervention significantly reduced the Ae. aegypti relative abundance (P < 0.002). Our results demonstrated that the combined insecticide interventions on two consecutive weeks significantly reduced the relative abundance of Ae. aegypti. This result indicated that the larvicide was successfully propelled reaching cryptical and difficult to reach aquatic habitats. However, even though the number of mosquitoes was greatly reduced, it was still greatly above the 10-mosquito threshold by trap night used by the Miami-Dade Mosquito Control Division to deploy an inspector to survey the area. Considering the lack of new and effective mosquito control tools, efficient and mobile insecticide propellers such as Buffalo Turbine can be of great help to manage mosquito populations in urban areas.

scholarly journals Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti

2019 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Makkiah Makkiah ◽  
Christina L Salaki ◽  
Berty Assa
Keyword(s):  
Experimental Study ◽  
Regression Analysis ◽  
Aedes Aegypti ◽  
Crude Extract ◽  
Larval Stage ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Probit Regression ◽  
Extract Concentration ◽  
Cymbopogon Nardus

Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti (The Effectiveness of Citronella Extract (Cymbopogon nardus) as Larvaside of Aedes aegypti) Makkiah*), Christina L Salaki **), Berty AssaProgram Studi Entomologi Pascasarjana Universitas Sam RatulangiManado, 95155*Email: [email protected],**Email korespondensi: [email protected]  (Article History: Received 20-10-2019; Revised 27-11-2019; Accepted 30-12-2019) AbstrakPengendalian nyamuk sebagai vektor utama penularan penyakit DBD hanya dapat dilakukan dengan cara memutuskan rantai penularannya melalui pengendalian nyamuk. Pengendalian nyamuk dapat dilakukan pada tahap larva dengan menggunakan biolarvasida yang terbuat dari bahan alami dan salah satunya adalah dengan menggunakan ekstrak kasar dari tanaman serai wangi (Cymbopogon nardus). Penelitian ini bertujuan untuk menguji efektivitas ekstrak serai wangi sebagai larvasida larva Aedes aegypti instar III dan IV. Penelitian ini adalah penelitian eksperimental menggunakan rancangan acak lengkap. Larva yang digunakan adalah larva instar III dan IV, dengan  perlakuan konsentrasi ekstrak serai wangi 20%, 30%, 40%, dan 50% dalam 250 ml air. Pada penelitian ini terdapat kontrol yaitu aquades yang tidak ditambahkan larutan apapun. Pengamatan kematian larva dilakukan pada jam ke 1, 3, 6, 12, 24, dan jam ke 48 dengan replikasi sebanyak 4 kali. Data presentase mortalitas larva digunakan untuk menghitung nilai LC50 dan LT50 dengan menggunakan analisisi Probit. Hasil penelitian menunjukkan ekstrak serai wangi efektif dalam mematikan 50% dari populasi  larva uji  dengan  nilai LC50 pada konsentrasi 36,48%serta waktu yang di butuhkan untuk mematikan  50% populasi larva uji adalah 10,45 jam. Kata kunci : Ekstrak, Cymbopogon nardus, larvasida, Aedes aegypti AbstractControl of mosquitoes as the main vector of transmission of DHF can only be done by breaking the chain of transmission through mosquito control. Mosquito control can be carried out at the larval stage by using biolarvasides made from natural materials, one of which is by using a crude extract from the citronella plant (Cymbopogon nardus). This research is to find out the effectiveness of citronella extract as larvae of Aedes aegypti larvae instar III and IV. This research is a pure experimental study using a complete random design. The larvae used were instar larvae III and IV, with the treatment of citronella extract concentration of 20%, 30%, 40%, and 50% in 250 ml of water. In this study, there was a control i.e. aquades which did not add any solution. Observation of larvae death was done at 1 hour, 2 hours. 4 hours, 12 hours, 24 hours and 28 hours with replication 4 times. Data on larval mortality were used to calculate LC50 and LT 50 values using Probit regression analysis. The results showed that citronella extract was effective in killing 50% of the population of test larvae with LC50 values at a concentration of 36.48% and the time needed to kill 50% of the population of test larvae was 10,450 hours.Keywords:  Extract, Cymbopogon nardus, larvasida, Aedes aegypti

scholarly journals Larvicidal potentiality, longevity and fecundity inhibitory activities of Bacillus sphaericus (Bs G3-IV) on vector mosquitoes, Aedes aegypti and Culex quinquefasciatus

2012 ◽  
Vol 44 (3) ◽  
pp. 15 ◽  
Author(s):  
Arjunan Nareshkumar ◽  
Kadarkarai Murugan ◽  
Indra Baruah ◽  
Pari Madhiyazhagan ◽  
Thiyagarajan Nataraj
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Bacillus Sphaericus ◽  
Control Agent ◽  
Vector Borne Diseases ◽  
Vector Population ◽  
Intervention Measures ◽  
Vector Borne

Intervention measures to control the transmission of vector-borne diseases include control of the vector population. In mosquito control, synthetic insecticides used against both the larvae (larvicides) and adults (adulticides) create numerous problems, such as environmental pollution, insecticide resistance and toxic hazards to humans. In the present study, a bacterial pesticide,<em> Bacillus sphaericus</em> (Bs G3-IV), was used to control the dengue and filarial vectors, <em>Aedes aegypti</em> and <em>Culex quinquefasciatus</em>. <em>Bacillus sphaericus </em>(Bs G3-IV) was very effective against<em> Aedes aegypti</em> and <em>Culex quinquefasciatus</em>, showing significant larval mortality. Evaluated lethal concentrations (LC<sub>50</sub> and LC<sub>90</sub>) were age-dependent, with early instars requiring a lower concentration compared with later stages of mosquitoes. <em>Culex quinquefasciatus</em> was more susceptible to <em>Bacillus sphaericus</em> (Bs G3-IV) than was <em>Aedes aegypti</em>. Fecundity rate was highly reduced after treatment with different concentrations of <em>Bacillus sphaericus</em> (Bs G3-IV). Larval and pupal longevity both decreased after treatment with <em>Bacillus sphaericus</em> (Bs G3-IV), total number of days was lower in the <em>B. sphaericus</em> treatments compared with the control. Our results show the bacterial pesticide <em>Bacillus sphaericus </em>(Bs G3-IV) to be an effective mosquito control agent that can be used for more integrated pest management programs.

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