Evaluation of the Ethyl Acetate Extract of the Roots of Avicennia marina as Potential Anticancer Drug

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

Download Full-text

Invitro Anticancer Activity of Ethyl Acetate extract of Trichopus zeylanicus. Ssp. Travancoricus Gaertn on Human Cervical Cancer Cell Line HeLa

Asian journal of biochemical and pharmaceutical research ◽

10.24214/ajbpr/9/1/3542 ◽

2019 ◽

Vol 9 (1) ◽

Keyword(s):

Cervical Cancer ◽

Ethyl Acetate ◽

Anticancer Activity ◽

Ethyl Acetate Extract ◽

Cancer Cell Line ◽

Cervical Cancer Cell Line ◽

Cell Line Hela ◽

Human Cervical Cancer Cell ◽

Line Hela ◽

Human Cervical Cancer

Download Full-text

Antioxidant and chemical constituents of ethyl acetate extract of Tamarix aphylla leaveas in Saudi Arabia

Pakistan Journal of Botany ◽

10.30848/pjb2020-6(33) ◽

2020 ◽

Vol 52 (6) ◽

Author(s):

Monira R. Al-Othman ◽

Fatimh S Alkhattaf ◽

Abeer R. Abd El-Aziz

Keyword(s):

Saudi Arabia ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents

Download Full-text

Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

Phytothérapie ◽

10.3166/phyto-2018-0106 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S119-S129

Author(s):

I. Namoune ◽

B. Khettal ◽

A.M. Assaf ◽

S. Elhayek ◽

L. Arrar

Keyword(s):

Ethyl Acetate ◽

Methanolic Extract ◽

Ethyl Acetate Extract ◽

Interleukin 1 ◽

Total Phenolic ◽

Dependent Manner ◽

Aqueous Extracts ◽

Traditional Use ◽

Marrubium Vulgare ◽

Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

Download Full-text

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

Current Drug Discovery Technologies ◽

10.2174/1570163817666200727101300 ◽

2020 ◽

Vol 17 ◽

Author(s):

Shubhaisi Das ◽

Sunanda Burman ◽

Goutam Chandra

Keyword(s):

Ethyl Acetate ◽

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Positive Control ◽

Leaf Extracts ◽

Ms Analysis ◽

Ft Ir ◽

Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

Download Full-text

Phytochemical Screening, Antimicrobial and Antioxidant Activities of Selected Mangrove Species

Current Bioactive Compounds ◽

10.2174/1573407214666180808121118 ◽

2020 ◽

Vol 16 (2) ◽

pp. 152-163 ◽

Cited By ~ 2

Author(s):

Nilesh L. Dahibhate ◽

Utpal Roy ◽

Kundan Kumar

Keyword(s):

Staphylococcus Epidermidis ◽

Methanol Extract ◽

Ethyl Acetate Extract ◽

Antioxidant Activities ◽

Avicennia Marina ◽

Total Flavonoids ◽

Phytochemical Screening ◽

Mangrove Species ◽

Sonneratia Caseolaris ◽

Bruguiera Gymnorhiza

Background: Mangrove plant extracts are used in folkloric medicine as aphrodisiac, sedative, antioxidant, antimicrobial and antimalarial. Screening for antimicrobial, phytochemical and antioxidant activities of fourteen mangroves plant species (Aegiceras corniculatum, Acanthus ilicifolius, Avicennia alba, Avicennia marina, Avicennia officinalis, Bruguiera cylindrica, Bruguiera gymnorhiza, Ceriops tagel, Excoecaria agallocha, Kandelia candel, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, and Sonneratia caseolaris) in various solvents are reported in this work. Methods: The antimicrobial screening was carried out using agar well diffusion method. In this study, nine pathogenic strains were used, including three Gram-positive and six Gram-negative bacteria. Phytochemical screening, total flavonoids, total phenolic and antioxidant activity was tested by DPPH radical scavenging assay. Four phenolic compounds (gallic acid, vanillin, tannic acid and quercetin) were quantified by LC-MS/MS in selected mangrove species. Results: Antimicrobial screening showed Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa were most susceptible to ethyl acetate extract as compared to ethanol and methanol extract. Ethyl acetate extract of Avicennia marina and Bruguiera gymnorhiza showed strong antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of saponins, phenolics, flavonoids, alkaloids, tannins, and terpenoids, which was found to be variable as per the solvent used for extraction. In addition, total phenolics and total flavonoids content with different solvents were found in the range of 11.08 to 196.76 mg GAE/g and 12.92 to 110.3 mg QE/g of extract respectively. Moreover, antioxidant capacities expressed in terms of IC50 (mg/mL) showed that methanol extract exhibited higher antioxidant capacity followed by ethanol extract. LC-MS/MS analysis showed gallic acid and tannic acid are present in higher concentration in Aegiceras corniculatum and Sonneratia caseolaris than other species. The vanillin and quercetin were found in the least concentration. Conclusion: Mangroves species are rich source of antioxidant, phenolics and antimicrobial compounds.

Download Full-text

Metabolite fingerprinting of novel Streptomyces UK-238 from the Himalayan forest

Current Pharmaceutical Analysis ◽

10.2174/1573412916666200206160836 ◽

2020 ◽

Vol 16 ◽

Author(s):

Nidhi Srivastava ◽

Indira P. Sarethy

Keyword(s):

Ethyl Acetate ◽

16S Rdna ◽

Retention Index ◽

Ethyl Acetate Extract ◽

Similarity Index ◽

Antimicrobial Drug ◽

16S Rdna Sequencing ◽

Metabolite Fingerprinting ◽

Streptomyces Sp ◽

Rdna Sequencing

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

Download Full-text