scholarly journals Histopathological Changes in Some Internal Organs Of White Mice Due To Treatment With Pentoxifylline

2011 ◽  
Vol 35 (2) ◽  
pp. 14-21
Author(s):  
Salema L . Hassan
Keyword(s):  
Mononuclear Cells ◽  
Liver Parenchyma ◽  
Lymphoid Hyperplasia ◽  
Control Group ◽  
White Pulp ◽  
Interstitial Tissue ◽  
Renal Tubules ◽  
Histopathological Changes ◽  
Internal Organs ◽  
Albino Mice

The aim of the study was to make knowledge on the histopathological changes in some internal organs ( liver, kidney and spleen) of albino mice after treatment with therapeutic dose(16mg/kg BW/day) of pentoxifylline (PTX). Thirty albino mice which are approximately at same age (8week) and body weight were, randomly divided into three equal groups, group 1:Received tape water along the period of experiment and considered as a control group, Group 2:Treated with Pentoxifylline ( 16 mg /kgBW/day)for 30 days Group 3:Treated with Pentoxifylline ( 16 mg/kgBW/days)for 60 day. The histopathological findings of liver, kidney and spleen, showed infiltration of mononuclear cells within the liver parenchyma and portal areas and in the interstitial tissue of the kidney with perivascular lymphocytic cuffing and mild degenerative changes represented by acute cellular swelling of hepatocytes and epithelial cells lining the cortical renal tubules in addition to congestion of blood vessels Spleen showed lymphoid hyperplasia of white pulp with congestion and infiltration of lymphocytes in red pulp.

scholarly journals COMPARATIVE STUDY OF PATHOGENICITY BETWEEN ASPERGILLUS FUMIGATUS AND PENICILLIUM CHRYSOGENUM IN MICE

2020 ◽  
Vol 51 (Special) ◽  
Author(s):  
S. N. Yassein
Keyword(s):  
Aspergillus Fumigatus ◽  
Penicillium Chrysogenum ◽  
Control Group ◽  
Histopathological Changes ◽  
Active Components ◽  
Internal Organs ◽  
Opportunistic Fungi ◽  
Liver And Kidney ◽  
Kidney Functions ◽  
Intraperitoneal Inoculation

This study was conducted to compare between two types of opportunistic fungi (Aspergillus fumigatus and Penicillium chrysogenum) in concerning their pathogenicity after intraperitoneal inoculation of mice. A total of twenty four male albino mice were used in this study which divided equally into 3 groups, The first and second groups were inoculated with 0.2ml of 1x 107spores/ml of A. fumigatus and P. chrysogenum  intraperitonially respectively, while the third group was inoculated with normal saline which served as control group. All animals were monitored for 2 weeks after infection. The blood samples were collected by heart puncture after 18 days post infection to isolate of serum that used for biochemical analysis of liver and kidney functions. After that, all animals were sacrificed. Some internal organs of infected groups (liver, kidney, intestine, heart, spleen and lung) were taken to study the histopathological changes. It was found that there was severe histopathological changes in studied organs of infected mice particularly liver, kidney, spleen and intestine which corresponding with significant variation (p<0.01) in enzyme activities of liver and kidney like (Alanine Aminotransferase (ALT), Urea and Creatinine). Also, It was found that P. chrysogenum had more impact on these enzymes (15.65 ± 0.78, 135.23 ± 8.75 and 0.928 ± 0.02 respectively) than A. fumigatus (21.70 ± 1.04, 57.91 ± 5.99 and 0.587 ± 0.03 respectively). Therefore, the present study indicated that fungi present in the environment can induce severe inflammation reach to tissue damage in most vital internal organs So, further studies should be performed to determine the specific virulence factors and active components, which are responsible for pathogenesis of A. fumigatus and P. chrysogenum in spite of the fact that P. chrysogenum can produce antibiotic.

scholarly journals Study of histopathological changes in brian of mice infected with Toxoplasma gondii isolated from domestic rabbit

2015 ◽  
Vol 39 (1) ◽  
pp. 90-93
Author(s):  
Raghad Ibraheam Khalil AL-Mahdawi
Keyword(s):  
Toxoplasma Gondii ◽  
Inflammatory Cells ◽  
Control Group ◽  
Histopathological Changes ◽  
Internal Organs ◽  
Domestic Rabbit ◽  
Group 2 ◽  
The Brain ◽  
Dark Blue ◽  
Intraperitoneal Inoculation

     The study was designed to determine the infection rate of Toxoplasma gondii in the rabbits and histopathological changes of infected internal organs in mice. A total of 60 blood samples were collected from clinically healthy rabbits from different region of Baghdad city for the serological detection of T. gondii infection. Biological assay in mice was performed by intraperitoneal inoculation of 0.1 ml digested organs suspension, (lungs, heart, liver, spleen, kidney, muscles, and brain) during the period from October 2013 until July 2014. The results revealed histopathological changes in bioassay mice infected by T. gondii isolated from domestic rabbit, no histopathological changes in the brain of animals of first group1 (control group), but in the group 2 (killed at day 15) the main pathological changes were perivascular perineuronal edema with presence of some degenerated neurons characterized  by shrunken dark blue stained (basophilic) cell bodies. In the animals of the group 2 (killed at day 21) the main brain histopathological changes were Sever congestion of meningeal blood vessels with infiltration of inflammatory cells and focal aggregation of microglial cells with diffuse obvious focal gliosis, whereas, in the same group animals that killed at day 28 the main brain histopathological changes were focal encephalomalacia and edema between molecular and granular layer.

scholarly journals Histopathological Effects of Prazosin Drug on Lung of Rats

2021 ◽  
Vol 2 (1) ◽  
pp. 25-28
Author(s):  
Dr. Zainab Sajid Mohammed
Keyword(s):  
Pulmonary Artery ◽  
Degenerative Change ◽  
Male Rats ◽  
Control Group ◽  
White Pulp ◽  
Histopathological Changes ◽  
Tunica Media ◽  
Graduate Department ◽  
Severe Hemorrhage

This study was conducted at the laboratory of histology and anatomy, Faculty of Medical and Health Techniques/Kufa, and laboratory of post Graduate/ Department of biology, Faculty of Science/University of Kufa, The present study was conducted to investigate the effect of Prazosin hydrochloride on some organs in male rats (Rattus norvegicus), about 25  mature male rats with the average body weight of 210-290gram and three months age were randomly divided into four groups (5rats / group). The first group was given orally with distilled water as a control group and the other groups (second, third, and fourth) were also given orally with three doses of Prazosin (25, 50,75 mg /kg. b.wt.) daily for a period of eight weeks.  At the end of the treatment period (eight weeks), rats were sacrificed, blood samples obtained, and organs lung, and spleen. The histopathological changes of lungs in the rats treated with prazosin at dose 25 mg/kg.b.wt for 8-weeks showed emphysema and dilation in some of the alveoli, hemorrhage distributed inside the tissue of the lung, polymorphic nuclear infiltration due to pneumonia, the pulmonary artery revealed degenerative changes in the tunica media structure (smooth muscle) and hyperplasia in the connective tissue around pulmonary artery and alveoli. These symptoms which occur in rats treated with prazosin at dose 50 and 75 mg/kg b.wt. as well as the histopathological changes of rat lung demonstrate severe hemorrhage, emphysema, thickening in the wall of some alveoli, pneumocyte necrosis (pneumocyte type 1 and pneumocyte type 2), and showed exudate among lung tissue. Histopathological changes of Spleen in the rats treated with prazosin at doses(50 and75)mg/kg b.w. for 8-weeks revealed histopathological changes, which represented by proliferation in the white pulp lead to fused white pulp together and destruction of some components of red pulp, stenosis in many splenic venous sinuses, the germinal artery show thickening in the tunica media and stenosis occurs, degenerative change in many nuclei of lymphocytes, and proliferation in the component of the white pulp.

scholarly journals Study of important moulds isolated from milk and its products in Baghdad – with experimental study the pathogenesis of Aspergillus Fumigatus moulds in mice

2005 ◽  
Vol 29 (2) ◽  
pp. 67-80
Author(s):  
Bushra I. Al-Kaisie
Keyword(s):  
Experimental Study ◽  
Dairy Products ◽  
Dairy Product ◽  
Clinical Signs ◽  
Raw Milk ◽  
Control Group ◽  
Histopathological Changes ◽  
Pathological Study ◽  
Internal Organs ◽  
Group Control

This study was designed to isolate and identify the type of moulds and theirpercentage of distribution with pathological study of highly moulds isolated from(130)samples were included in this study with divided into (30)samples driedmilk,(30).samples baby food ,(30)samples raw milk , (40) samples dairy products .The results revealed the percentage of infection according to total count ofisolation in raw milk were 100 % dairy product 65 %. The most common fungiisolated from raw milk were Asp.niger of 16.2 % Asp. fumigatus of 15 % Asp.flavusof 13.7 % Pencillium spp. 12.5 % . From dairy products were isolated withpercentage of Asp. Fumigatus 34.9% Geotrichum spp . of 23 % ,Asp. nager of 11.5% . Pathological study was carried out to investigate the clinical signs with grosslyand Histopathological changes inoculated with most pathogenic moulds Asp.fumigatus (30)male white swiss mice were used in this study and divided into fourgroups . The first group were injected I/P with 0.2 ml of Asp. fumigatus containing1X105 spores / ml , 4th group (control)given orally 0.2 ml of PBs.Two mice fromeach treated groups and one from each control group were scarified on week (1,2,3,4).the clinical manifestation of the 1st group were at 2nd week showed dyspnea .Mouldisolated from blood and internal organs at third week , with no isolation from 3rdgroup. The Histopathological observations showed presence of degeneration ,necrosis and diffused granuloma in liver, lung , spleen , kidney and hemorrhage withthrombosis in brain and intestine .

scholarly journals The effect of usage of Artemisia herba alba and Urtica dioica herbs extracts on the histopathological changes of Eimeria tenella parasite in the broiler chick.

2012 ◽  
Vol 36 (0A) ◽  
pp. 165-173
Author(s):  
Haider Mohammad Ali Al-Rubaie1
Keyword(s):  
Mononuclear Cells ◽  
Histopathological Examination ◽  
Biological Effects ◽  
Water Extract ◽  
Treated Group ◽  
Eimeria Tenella ◽  
Urtica Dioica ◽  
Control Group ◽  
Epithelial Layer ◽  
Histopathological Changes

This study was conducted to determine the biological effects of both Artemisia herba alba andUrtica dioica on the pathogenesis of Eimeria tenella, so 150 broilers, 7days old (Ross breed)were used, divided randomly into 6 groups. The first fourth groups include 30 chicks for eachgroup, while fifth and sixth groups have 15 chicks for each, consider as positive and negativegroups.The first five groups infected orally with 1000 oocysts of E.tenella directly into the cropat 17 days old. Histopathological examination of cecae in the treated groups with Urtica dioicarevealed moderate mononuclear cells (MNCs) infiltration, while sever lymphocytic aggregationswere seen in the treated group of water extract comparing with less cellular inflammatoryinfiltration was seen in the treated group with Artemisia herba alba .Severe degeneration andnecrotic changes were appeared in epithelial layer of mucus glands as well as appearance ofdegenerated schizonts in the section of the control group.

scholarly journals Histostructure of the internal organs and skin of rats for the long-term effect of the drug “VetMikoDerm”

2019 ◽  
Vol 21 (94) ◽  
pp. 136-141
Author(s):  
V. P. Martynyshyn
Keyword(s):  
Skin Toxicity ◽  
Total Surface Area ◽  
Control Group ◽  
Histological Structure ◽  
Renal Tubules ◽  
Internal Organs ◽  
Laboratory Rats ◽  
Long Term Effect ◽  
Long Time ◽  
Experimental Group

The investigation of the skin toxicity of the liniment “VetMikoDerm” for prolonged use was performed on young and healthy laboratory rats with intact skin, body weight 200–220 g. The investigated environment was applied to a clean, non-woolly area (dorsal/lateral surface) of at least 10% of the total surface area. The spotted thistle oil was applied to the pre-prepared area of the skin of the animals of the control group, and the other two experimental groups of animals got the studied drug at the doses of 50 (I) and 500 mg/kg (II), respectively. The drug was applied to the skin daily for 28 days. At the end of the experiment, after the decapitation of the rats conducted under the light etheric anesthesia, a complete pathoanatomical dissection was performed, the coefficients of the mass of the internal organs were determined, samples of liver, kidney and skin tissues were taken for their histological examination. According to the results it was established that the liver and kidneys histology, and skin of rats of the 1st experimental group were similar to those that were of the control animals. At the same time, receiving the maximum dose (10 times the therapeutic dose) of the drug “VetMikoDerm”, the majority of rats of the 2nd group showed a discomplication of the lamellar structure of the liver lobules, hepatocytes were placed in separate groups, sinusoidal capillaries were expanded. The presence of hepatocytes with heterogeneous, granular and weakly colored cytoplasm was observed in the central parts of the lobules, the nuclei of individual hepatocytes were increased, indicating the development of granular protein degeneration of the parenchyma. Histologically, under these conditions, foci of granular dystrophy of the epithelium of the vorticular and direct renal tubules with the expansion of their lumen were found in the structure of kidney of rats. The kernels of individual nephropyelitis had signs of karyopicnosis and cariorexis. The histological structure of skin of rats in all experimental groups was of the same type and consisted of the epidermis, dermis and hypodermis. Animals of the 2nd experimental group which repeatedly and for a long time were applied a “VetMikoDerm” lineulation in a dose that exceeded its optimal amount by 10 times showed the epidermal thickening, the presence of small-mesenchymal polymorphocytic infiltration, microcirculatory corneal disorder and sebaceous gland hyperplasia  glands in the dermis that indicated the development of a compensatory-adaptive reaction in places of application of a 10-fold dose of the investigational medicinal product.

scholarly journals Effect of the Silver Nanoparticles on the Histology of Albino Mice Ovaries

2017 ◽  
Vol 14 (4) ◽  
pp. 662-668
Author(s):  
Baghdad Science Journal
Keyword(s):  
Silver Nanoparticles ◽  
Blood Vessels ◽  
Normal Saline ◽  
Fatty Degeneration ◽  
Control Group ◽  
Histopathological Changes ◽  
Follicular Cells ◽  
Histological Changes ◽  
Adult Females ◽  
Albino Mice

The aim of study is to identify the histological changes in ovaries of the albino mice exposed to silver nanoparticles. Sixty adult females were collected and exposures by 4000 p.p.m. and 50-150 nm in size, Females were divided into 3treated groups. The concentration dosage was (1, 1.5 and 2) p.p.m. of silver nanoparticles for 7, 14 and 21 days as exposure periods as well as control group which treated by normal saline. Treated groups appeared different histopathological changes, it is depending on the concentration of silver nanoparticles and the period of exposure. These changes were included congestion in the blood vessels, hemorrhage, hyaline degeneration, fatty degeneration, pyknosis, necrosis as well as fusion of cells in follicular cells, amyloid proteins in the blood vessels and Fatty necrosis.

Effects of toxic Microcystis aeruginosa bloom on liver of nile tilapia (Oreochromis niloticus)

2017 ◽  
Vol 45 (1) ◽  
pp. 1-10
Author(s):  
Sumaiya Ahmed ◽  
Bernd Giese ◽  
Volker Schulz ◽  
Md Sagir Ahmed
Keyword(s):  
Microcystis Aeruginosa ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fish Tissue ◽  
Histopathological Change ◽  
Control Group ◽  
Histopathological Changes ◽  
Internal Organs ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Vascular Proliferation

The effects of toxic Microcystis aeruginosa on the liver of Nile tilapia, Oreochromis niloticus were investigated in the laboratory. Four treatments were setup into aquariums for 15 days to investigate the histopathological changes exposed to Microcystis aeruginosa bloom. Fishes of treatment A1 were treated as a control group and given artificial feed. In the three treatments (A2, A3 and A4), the M. aeruginosa cell concentration were 35 × 102, 72 × 102, 149 × 102 colony/ml, respectively. In the control group, no histopathological change was observed. In exposed fish, histopathological alterations were characterized by swollen and granular cytoplasm, vascular proliferation, bile stasis, fatty change and focal necrosis. Histopathological changes were observed within five days of exposure when fish exposed to moderate (72 × 102 colony/ml) to high (149 × 102 colony/ml) bloom concentration, whereas it took 15 days in lower concentration (35 × 102 colony/ml). Histopathological changes proved that intake of toxic M. aeruginosa bloom by aquatic animals, particularly Nile tilapia has significant effects on its internal organs that may cause of massive mortality. Accordingly, cyanotoxin accumulation in fish tissue my pose a risk to human health through the food chain.Bangladesh J. Zool. 45(1): 1-10, 2017

scholarly journals Histopathological Changes caused by The Chronic Effect of Nitrofurantoin Drug in The Testes of Albino Mice

2015 ◽  
Vol 12 (1) ◽  
pp. 20-26
Author(s):  
Baghdad Science Journal
Keyword(s):  
Germ Cell ◽  
Cell Layer ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Sperm Number ◽  
Chronic Effect ◽  
Albino Mice ◽  
High Concentrations

The present study was conducted to determine histopathological changes caused by chronic effect of Nitrofurantoin(NFT) in The albino mice Testes. The Study included 40 mice were divided on the five groups: the first group taken distilled water and become control group . the remaining group which are exposure with NFT drug in concentration (100-150-200-250) mg / kg, respectively, Doses were given orally for a period (month and two months). The results of histopathological changes included occurrence of congestion in the blood vessel and degeneration of spermatogonia and aggregation of spermatids in the lumen of semineferous tubules and inhibition of spermatogensis process and decrease of sperm inside the lumen of semineferous tubule as well as necrosis and atrophy within germ cell layer , this changes be greatest in groups with high concentrations (200-250) mg / kg. NFT drug have side effect on testes by inhibition the spermatogenesis process and decrease of mature sperm number and necrosis found within in germ cell layer lining the seminiferous tubules.

scholarly journals Histopathological changes in the internal organs of female striped gourami (Trichogaster fasciata) exposed to sub-lethal concentrations of salt water

2021 ◽  
Vol 32 (2) ◽  
pp. 237-244
Author(s):  
WATINI REZA ◽  
MD. TAREQUL ISLAM ◽  
MD. HELAL UDDIN ◽  
KIZAR AHMED SUMON ◽  
HARUNUR RASHID
Keyword(s):  
Water Exchange ◽  
Renal Tubule ◽  
Salt Water ◽  
Kidney Tissue ◽  
Control Group ◽  
Histopathological Changes ◽  
Fish Liver ◽  
Internal Organs ◽  
Hepatic Rupture ◽  
Water Holding

The present study was conducted to evaluate the effects of climate induced salinity on histoarchitecture of liver, kidney and ovary tissue of female striped gourami (Trichogaster fasciata) exposed tothree sub-lethal concentrations (3, 6 and 9 ppt) of crude salt along with a control group (0 ppt) for a periodof 30 days. The 96-h LC50 value of crude salt for striped gourami was previously estimated as 11.107 ppt. Toconduct the experiment 12 plastic tanks each having 70L water holding capacity were used for the fourtreatments with three replications for each. Water exchange was done on every 10th day and crude salt wasused to maintain desired salinity. Several histopathological changes were observed in fish liver, kidney andovary exposed to different concentrations of salinity. Increasing necrosis, hemorrhage, hepatic rupture,melano-macrophage cells, degenerated cell and vacuums in the liver tissue were found in salt water treatedgroups. Similarly, a number of changes such as, vacuole, cellular degeneration, hemorrhage, disintegratedglomeruli, destructed renal tubule and extensive vacuolein were observed in the kidney tissue of salt watertreated groups. Degenerated oocyte, inter-follicular space, disrupted oocytes, etc. were observed in ovarywith increasing concentration of salinity and time of exposures. Physiological and metabolic dysfunctionsmight have happened to female stripped gourami due to changes in vital internal organs like liver, kidney andovary. Therefore, the present research further suggested that climate induced salinity intrusion is capable ofstressing freshwater fishes resulting in deformities of liver, kidney and ovary.

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