scholarly journals Histopathological Changes caused by The Chronic Effect of Nitrofurantoin Drug in The Testes of Albino Mice

2015 ◽  
Vol 12 (1) ◽  
pp. 20-26
Author(s):  
Baghdad Science Journal
Keyword(s):  
Germ Cell ◽  
Cell Layer ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Sperm Number ◽  
Chronic Effect ◽  
Albino Mice ◽  
High Concentrations

The present study was conducted to determine histopathological changes caused by chronic effect of Nitrofurantoin(NFT) in The albino mice Testes. The Study included 40 mice were divided on the five groups: the first group taken distilled water and become control group . the remaining group which are exposure with NFT drug in concentration (100-150-200-250) mg / kg, respectively, Doses were given orally for a period (month and two months). The results of histopathological changes included occurrence of congestion in the blood vessel and degeneration of spermatogonia and aggregation of spermatids in the lumen of semineferous tubules and inhibition of spermatogensis process and decrease of sperm inside the lumen of semineferous tubule as well as necrosis and atrophy within germ cell layer , this changes be greatest in groups with high concentrations (200-250) mg / kg. NFT drug have side effect on testes by inhibition the spermatogenesis process and decrease of mature sperm number and necrosis found within in germ cell layer lining the seminiferous tubules.

scholarly journals METRONIDAZOLE INDUCES SIGNIFICANT PATHOLOGICAL ALTERATIONS IN THE MALE REPRODUCTIVE SYSTEM OF MICE

2021 ◽  
Vol 52 (6) ◽  
pp. 1375-1381
Author(s):  
Z. K. Al – Timimi
Keyword(s):  
Reproductive System ◽  
Sperm Morphology ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Negative Effects ◽  
The Mean ◽  
Water Treated Control

The present study was aimed to investigate the possible effects caused by the antibiotic, metronidazole, on the histology of the testis and the morphology of the sperms of mice. To achieve this,  adult male albino Swiss Mice were orally administrated with 0.1 ml (100 mg\ kg ) of the commercially used metronidazole (Flagyl) for 60 consecutive days. The results showed significant decreases (p<0.05) in the mean weight of body and testis in the treated mice as compared to the distilled water-treated control group. The treatment also caused several histopathological changes in the testis which included necrosis, congestion, hemorrhage, edema, germ cell downfall  and rupture in the seminiferous tubules. The tests of the sperm morphology revealed a number of deformations as a result of the treatment, such as the appearance of headless,  bend, broken-tailed, hook-tailed, and two-headed sperms, as well as sperms with cytoplasmic droplets. We conclude that from these results that orally administered Metronidazole exerts negative effects on the male reproductive system of mice .

scholarly journals Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes

2009 ◽  
Vol 78 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Hayati Yuksel ◽  
Erkan Karadas ◽  
Hikmet Keles ◽  
Hasan Huseyin Demirel
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Male Rats ◽  
Control Group ◽  
High Dose ◽  
Histopathological Changes ◽  
Proliferation And Apoptosis ◽  
Group A ◽  
Higher Doses ◽  
Group 1

In this study, experimentally lindane-induced histopathological changes and proliferation and/or apoptosis in germ cells in the rat testes were investigated. A total of 40 healthy fertile 3-month-old male rats were used. Animals were divided into 4 groups, each containing 10 rats. Group 1 (control) was given only pure olive oil, Groups 2, 3 and 4 were administered lindane at 10, 20 and 40 mg/kg/bw, respectively, by gastric gavage for 30 days. Microscopically, degenerative changes were observed in the lindane-treated groups. For proliferative activity PCNA immunolabelling and for germ cells apoptosis TUNEL methods were performed. Although a strong PCNA positivity in the control group was observed, a gradual decrease was noted in the lindane-treated groups especially at higher doses. Significant increases of apoptosis were seen in the lindane-treated groups compared to the control group. A decrease in testosterone concentrations was observed in lindane-treated groups compared to the control group. The study indicates that high-dose lindane intoxication contributes to the suppression of spermatogenesis through a reduction of germ cell proliferation and an increase of germ cell death in rat testes.

scholarly journals Arsenic induced genotoxic and histopathological changes in male Swiss albino mice, Mus musculus

2011 ◽  
Vol 3 (2) ◽  
pp. 329-339
Author(s):  
Animesh K. Mohapatra ◽  
Deepika Rai ◽  
Anika Tyagi
Keyword(s):  
Arsenic Trioxide ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Hydropic Degeneration ◽  
Swiss Albino Mice ◽  
Cytoplasmic Vacuolization ◽  
Liver And Kidney ◽  
Albino Mice

The present study was carried out to investigate the effect of arsenic trioxide on the DNA and histomorphology of testis, liver and kidney of Swiss albino mice, Mus musculus. Oral administration of arsenic trioxide induced DNA damage in the testis, liver and kidney marked by light pink staining of nuclei after Feulgen’s reaction with reduced fine chromatin. Simultaneously severe histological changes were noted like distortion of seminiferous tubules, disorganization of spermatogonia, spermatocytes and spermatids with cytoplasmic vacuolization and nuclear pycnosis in testis. There was almost disappearance of sinusoids due to disruption of hepatic plates, inflammatory cellular infiltration around central veins and cytoplasmic vacuolization in hepatocytes with large irregular nuclei in liver of treated mice. Disorganized glomeruli with distorted Bowman’s capsules and mild to severe multifocal cloudy and hydropic degeneration with necrosis of tubules were observed in the kidney of treated mice. Inference drawn from the study indicated that arsenic induced both genotoxic histotoxic lesions.

Effects of Garcinia Cambogia on the Weights of Normal Albino Mice

2021 ◽  
Vol 15 (11) ◽  
pp. 2956-2957
Author(s):  
Gul Afshan ◽  
Ponum Mirani ◽  
Imtiaz Aslam ◽  
Sobia Ibrahim
Keyword(s):  
Control Group ◽  
P Value ◽  
Distilled Water ◽  
Garcinia Cambogia ◽  
Group Iii ◽  
Smart Drugs ◽  
Group I ◽  
Final Weight ◽  
Albino Mice ◽  
Experimental Group

Aim: Effects of available weight reducing drugs on the weights of a normal albino mice. Methodology: In this study, total 39 adult albino mice were used and were divided in three groups containing 13 animals in each. Group I served as control and was given 1 ml of distilled water once a day for 8 weeks. Group II and Group III served as experimental group and mice in these groups were given 0.5 mg of Slim Smart and Ultra Slim Plus drugs dissolved in 1 ml of distilled water respectively once a day for 8 weeks. To support these results weights of kidneys were also measured and relative tissue width index was calculated and compared with control group. Results: The weight of the animals increased in the experimental groups as compared to the control group. The overall difference for final weight among three groups was highly significant with p-value <0.01. When final weight compared group wise, the experimental groups had significantly higher weight as compared to control with p-values <0.01and 0.028 Conclusion: Both Ultra Slim Plus and Slim Smart drugs cause weight to increase in the individuals who are not obese and have BMI in normal range Keywords: Weight loss, Garcinia Cambodia, kidney, Albino mice

scholarly journals Histological Effects of Titanium Dioxide Nanoparticles Size 10 Nm in Mice Testes

2017 ◽  
Vol 5 (2) ◽  
pp. 158 ◽  
Author(s):  
Imad Taher Abdulla
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Testicular Tissue ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histological Changes ◽  
Low Concentrations ◽  
High Concentrations ◽  
Histological Effect

In the present study, the histological effect of titanium dioxide nanoparticles (TDN) on testicular tissue of mature Mus musculus mice was investigated. The animals were divided into six groups, control group treated with TDN free saline and five groups treated with TDN as follow 5, 10, 50, 100 and 150 mg/kg B.W. The results showed that TDN has histological effects on testicular tissue like sever congestion, mild edema between seminiferous tubules (STs) and decrease the thickness of germinal epithelium at low concentrations, While, the histological changes at high concentrations involved disturbance in STs diameters, sever edema between STs, sever vaculation in the germinal epithelium and necrosis in spermatogonia, germinal epithelium and Sertoli cells.

scholarly journals Effect glucosamine sulfate drug on liver tissue of male Albino mice

2015 ◽  
Vol 12 (2) ◽  
pp. 266-272
Author(s):  
Baghdad Science Journal
Keyword(s):  
Liver Tissue ◽  
Glucosamine Sulfate ◽  
The Other ◽  
Control Group ◽  
Distilled Water ◽  
Histological Changes ◽  
Albino Mice

The current study designed to determine the effect of Glucosamine sulfate on the liver tissue of Albino mice .the study included (40)mice divided in to 4 groups(control group had distilled water orally ).The other groups treated with(1000,2000,3000)ml/k .respectively for 8 week .the liver have been taken from dissected animal for microscopic preparation to study the histological changes .Frequently histopathologicale changes appeared in the liver tissue of the exposure groups during (4-8)week .This changes depends on (Dose and Time ). The effects were Congestion ,Infiltration ,Swelling ,Vaculation ,Hyalinization , Amyloid and Necrosis.

scholarly journals Effect of the Silver Nanoparticles on the Histology of Albino Mice Ovaries

2017 ◽  
Vol 14 (4) ◽  
pp. 662-668
Author(s):  
Baghdad Science Journal
Keyword(s):  
Silver Nanoparticles ◽  
Blood Vessels ◽  
Normal Saline ◽  
Fatty Degeneration ◽  
Control Group ◽  
Histopathological Changes ◽  
Follicular Cells ◽  
Histological Changes ◽  
Adult Females ◽  
Albino Mice

The aim of study is to identify the histological changes in ovaries of the albino mice exposed to silver nanoparticles. Sixty adult females were collected and exposures by 4000 p.p.m. and 50-150 nm in size, Females were divided into 3treated groups. The concentration dosage was (1, 1.5 and 2) p.p.m. of silver nanoparticles for 7, 14 and 21 days as exposure periods as well as control group which treated by normal saline. Treated groups appeared different histopathological changes, it is depending on the concentration of silver nanoparticles and the period of exposure. These changes were included congestion in the blood vessels, hemorrhage, hyaline degeneration, fatty degeneration, pyknosis, necrosis as well as fusion of cells in follicular cells, amyloid proteins in the blood vessels and Fatty necrosis.

scholarly journals Histopathological Changes in Some Internal Organs Of White Mice Due To Treatment With Pentoxifylline

2011 ◽  
Vol 35 (2) ◽  
pp. 14-21
Author(s):  
Salema L . Hassan
Keyword(s):  
Mononuclear Cells ◽  
Liver Parenchyma ◽  
Lymphoid Hyperplasia ◽  
Control Group ◽  
White Pulp ◽  
Interstitial Tissue ◽  
Renal Tubules ◽  
Histopathological Changes ◽  
Internal Organs ◽  
Albino Mice

The aim of the study was to make knowledge on the histopathological changes in some internal organs ( liver, kidney and spleen) of albino mice after treatment with therapeutic dose(16mg/kg BW/day) of pentoxifylline (PTX). Thirty albino mice which are approximately at same age (8week) and body weight were, randomly divided into three equal groups, group 1:Received tape water along the period of experiment and considered as a control group, Group 2:Treated with Pentoxifylline ( 16 mg /kgBW/day)for 30 days Group 3:Treated with Pentoxifylline ( 16 mg/kgBW/days)for 60 day. The histopathological findings of liver, kidney and spleen, showed infiltration of mononuclear cells within the liver parenchyma and portal areas and in the interstitial tissue of the kidney with perivascular lymphocytic cuffing and mild degenerative changes represented by acute cellular swelling of hepatocytes and epithelial cells lining the cortical renal tubules in addition to congestion of blood vessels Spleen showed lymphoid hyperplasia of white pulp with congestion and infiltration of lymphocytes in red pulp.

scholarly journals Cytarabine Induced Reproductive Histopathological Changes in Albino Male Mice

2017 ◽  
Vol 11 (1) ◽  
pp. 6-12
Author(s):  
Niran A. Ibrahim ◽  
Hanady S. Al-Shmgani ◽  
Ruqaya Ibrahim
Keyword(s):  
Myeloid Leukemia ◽  
Leydig Cells ◽  
Chemotherapeutic Agent ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Histopathological Changes ◽  
Sperm Abnormality ◽  
Experimental Group

Cytarabine (Ara-C) is widely used as an effective chemotherapeutic agent, essentially in the treatment of acute myeloid leukemia. Albino male mice (Mus musculus) are used in this study to determine in vivo toxicity of the drug on spermatogenesis and sperm abnormality. Mice are divided into two groups; the experimental group received a dose of 0.33 mg/kg/day Ara-C for seven days, while the control group received normal saline for 7 days. Results indicated a significant increase in head abnormalities percentage (p≤ 0.05) in comparison to control group. Testosterone hormone concentration was significantly decreased (1.14 ± 0.12) ng/ml in treatment group in comparison with control group (2.41± 0.22) ng/ml. Histopathological changes revealed detectable spermatogenesis degeneration. Treatment caused spermatocytes disruption, presence of necrotic cells and appearance of spermiophages with slought cells in several seminiferous tubules. Leydig cells morphology are mostly intact; however, interstitial area appeared hypercellular. It is concluded that Ara-c exposure highly destructive to seminiferous tubule epithelium, which may lead in depressed mice fertility.

Histopathological effects of organometallic maneb on testis in rats: a light and electron microscopic study

2006 ◽  
Vol 22 (9) ◽  
pp. 395-398 ◽  
Author(s):  
Engin Deveci
Keyword(s):  
Endoplasmic Reticulum ◽  
Treatment Time ◽  
Male Rats ◽  
Whole Body ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Cell Organelles ◽  
Electron Microscopic ◽  
Orogastric Tube

Maneb is a dithiocarbamate fungicide that contains manganese. Dithiocarbamates are considered to be chemicals of low toxicity. An experimental group (n=10) received maneb (obtained from Hoecht-Shering) at a concentration of 250 ppm in distilled water five days a week (treatment time is three weeks). It was orally administered by an orogastric tube. Acute oral LD 50 for male rats has been found to be 6750 mg/kg. The control group (n=10) received distilled water. The whole body and testis weights of male rats were taken, and significant differences in body weight appeared between the control and treated groups (P<0.01). No statistical difference was found in the testis weights between the control and treated groups (P<0.05). Degeneration in spermatic cells can be seen in a few seminiferous tubules and vascularization. Transmission electron microscopy, interstitial cell and blood vessels of maneb-treated rat testis did not show any changes. Leydig cells with their large, excentric nuclei, cytoplasm with granular endoplasmic reticulum and other cell organelles appeared to be normal. Sertoli cells appeared unusually as a well developed rough endoplasmic reticulum and Golgi complexes had irregular compacted chromatin. Changes in Sertoli cell mitochondria included swelling and loss of cristae. Toxicology and Industrial Health 2006; 22: 395-398.

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