scholarly journals Efektivitas Waktu Persilangan Tiga Genotipe Cabai (Capcicum sp) pada Persilangan Dialel

2021 ◽  
Vol 17 (1) ◽  
pp. 9-14
Author(s):  
Retno D Andayani ◽  
Navita Maharani

Chili is an important horticultural commodity in Indonesia. However, its production is still not optimal because there are not many superior seeds available, which causes low productivity. One solution to this problem is to improve planting material (seeds) by a plant breeding program through a hybridization process. Crossing different types of chili requires the right time to increase the chances of success. The purpose of this study was to determine the effectiveness of crossing three genotypes of chili (two introduced and one local). The study was carried out with a factorial randomized design. The first factor was a combination of crosses of three parents, namely Sweet Italian, Garda and Katokkon by dialel crosses. The second factor was the time of crossing, namely at 04.00, 05.00, 06.00, 07.00, 08.00. The results of the analysis showed that the combination of parents had an interaction with crossing time in increasing the dsuccess of the crosses. Each parent combination hah a specific time for crossing. If the female parent was Sweet Italian, the time of crossing early in the morning (low temperature) was more optimal, which was between 04.00-07.00. For Katokkon×Garda crosses and their reciprocals, the percentage of successful crosses increased if it was done at 06.00-08.00. None of the crosses between Garda×Sweet Italian were successful or were entirely lethal. Keywords: chili, crossing time, dialele , introduction genotype   ABSTRAK Cabai merupakan komoditas hortikultura yang penting di Indonesia. Namun produksinya masih belum optimal karena belum banyak tersedia benih unggul, yang menyebabkan rendahnya produktivitas. Salah satu solusi masalah tersebut adalah dengan perbaikan bahan tanam (benih) melalui program pemuliaan tanaman dengan proses persilangan (hibridisasi). Persilangan cabai yang berbeda jenis memerlukan waktu yang tepat untuk dapat meningkatkan peluang keberhasilannya. Tujuan penelitian ini adalah untuk mengetahui efektivitas waktu persilangan tiga genotipe cabai (dua introduksi dan satu lokal). Penelitian dilaksanakan dengan rancangan acak kelompok faktorial, Faktor pertama adalah kombinasi persilangan dari tiga tetua, yaitu Sweet Italian, Garda dan Katokkon secara dialel. Faktor kedua adalah waktu persilangan, yaitu pada pukul 04.00, 05.00, 06.00, 07.00, 08.00. Hasil analisis menunjukkan bahwa kombinasi tetua memiliki interaksi dengan waktu persilangan dalam meningkatkan keberhasilan persilangan. Tiap kombinasi tetua memiliki waktu yang spesifik untuk persilangan. Jika tetua betina Sweet Italian, waktu persilangannya semakin pagi (suhu rendah) semakin optimal, yakni di antara pukul 04.00-07.00. Untuk persilangan Katokkon×Garda maupun resiproknya, persentase keberhasilan persilangan semakin meningkat jika dilakukan pada pukul 06.00-08.00. Tidak ada penyerbukan silang antara Garda×Sweet Italian yang berhasil persilangan atau seluruhnya letal. Kata kunci: cabai, dialel , genotipe introduksi, waktu persilangan

Agrivet ◽  
2021 ◽  
Vol 25 (2) ◽  
pp. 88
Author(s):  
Rina Srilestari ◽  
Suwardi Suwardi

The economic value of the Abaca banana is on the trunk which contains fiber for valuable textile and paper industry raw materials. The multipurpose fiber and its prospects are quite good, so the abaca plant gets a lot of attention from various community groups both private, state-owned, cooperative and farmers. Propagation through multiplication humps is very low, planting material is easily damaged in transportation, is not durable and requires large space so that transportation costs are high. One alternative to overcome the provision of healthy, large and fast seedlings is to use seedlings from tissue culture. The aim of the study is to get the right thiamin and leaf fertilizer concentrations at the acclimatization stage. The experimental design used was a 2-factor Complete Randomized Design, Thiamin concentration (1 ml / l, 2 ml / l, 3 ml / l) and leaf fertilizer (0.5 ml / l, 1 ml / l, 1.5 ml / l). The data obtained were analyzed for diversity at 5% level and further tests using Duncan Multiple Range Test (DMRT) level of 5%. The results showed no interaction between thiamin and leaf fertilizer, Thiamin 2 ml / l gave the best results on all parameters and 1.5 ml / l leaf fertilizer could increase plant height and the number of leaves of abaca banana plants.Keywords: Thiamin, leaf fertilizer, abaca banana, acclimatization


Omni-Akuatika ◽  
2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Istiyanto Samidjan ◽  
Diana Rachmawati

Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the  weight absolute, survival, FCR,  molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism  treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8)  respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.


Author(s):  
S. Edith Taylor ◽  
Patrick Echlin ◽  
May McKoon ◽  
Thomas L. Hayes

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 520e-520
Author(s):  
Juan E Manzano ◽  
Oswaldo Valor

Mango fruits `Criollo de Bocado' harvested at the mature-green stage were treated with a hydrothermic treatment of 55 °C for 3 min and stored for 20 days at temperatures of 10 ± 2, 15 ± 2 and 28 ± 2 °C. A randomized design 2 × 3 × 4 with three replications was used. Some chemical parameters were analyzed, such as total soluble solids content (% TSS), pH, tritatable acidity, and TSS/tritatable acidity ratio. TSS content increased with storage time at low temperature. The pH increased measurably with storage temperature, while tritatable acidity values results had inconsistent data.


2019 ◽  
Vol 32 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Luma Rayane de Lima Nunes ◽  
Paloma Rayane Pinheiro ◽  
Charles Lobo Pinheiro ◽  
Kelly Andressa Peres Lima ◽  
Alek Sandro Dutra

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.


Land ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 311
Author(s):  
Anna Granath Hansson ◽  
Peter Ekbäck ◽  
Jenny Paulsson

This paper aims to elucidate the sliding scale between usufruct and ownership by applying a property rights framework to three Swedish forms of tenure in multifamily housing. The framework deconstructs the bundles of rights of rental, tenant-ownership and ownership to highlight commonalities and differences connected to the right to use and exclude, the right to transfer and the right to the value. It is concluded that the three tenure forms have many traits in common but that there are distinct differences in some areas, most notably in connection to the right to the value. The property rights framework applied in the study may be applicable also on a general level as a method to analyze and compare tenures of different types in different countries. Further, ways to improve the framework and cover more facets of outcomes of property rights patterns are suggested.


2020 ◽  
Vol 147 ◽  
pp. 01015
Author(s):  
Dimas Fendy Pradana ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.


2014 ◽  
Vol 96 (2) ◽  
Author(s):  
Dominik Perler

Abstract:According to Spinoza, there is no categorical distinction between human and non-human animals: they all belong to the same nature and all consist of bodies with corresponding ideas. This thesis gives rise to two problems. How is it possible to distinguish different types of animals, in particular nonrational and rational ones, if all of them have the same metaphysical structure? And why does Spinoza nevertheless claim that human beings have a privileged status that gives them the right to use non-rational animals? This paper examines these two problems, arguing that the solution to both of them lies in Spinoza’s all-embracing naturalism.


2016 ◽  
Vol 19 (3) ◽  
pp. 432-439
Author(s):  
Melville Saayman ◽  
Waldo Krugell ◽  
Andrea Saayman

The Cape Argus Pick n Pay Cycle Tour is a major event on the road cycling calendar. The majority of cyclists travel significant distances and participation produces a substantial carbon footprint. This paper examines participants’ willingness to pay to offset their carbon footprint. The purpose of this paper is to make a contribution to the literature by linking willingness to pay to attitudes towards or beliefs (green views) about the initiatives in place, to ensure a greener cycle tour. Factor analysis is used to identify different types of cyclists, based on their green views: those with green money, those who prefer green products and the “re-cyclers”. The results of the regression analysis reveal that socio-demographic variables and the right attitude towards the environment are significant predictors of stated willingness to pay for climate change mitigation.


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