scholarly journals Engineering Technology Fish Farming of Snakeheads Channa striat ,Bloch, 1793) Based Feed Vitamin C Increase to Superior Quality Using Microsatellite

Omni-Akuatika ◽  
2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Istiyanto Samidjan ◽  
Diana Rachmawati
Keyword(s):  
Molecular Weight ◽  
Vitamin C ◽  
Fish Farming ◽  
Engineering Technology ◽  
Randomized Design ◽  
Different Types ◽  
Data Arrangement ◽  
Completely Randomized Design ◽  
The Right ◽  
Different Doses

Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the  weight absolute, survival, FCR,  molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism  treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8)  respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.

scholarly journals Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Daun Kejompot (Crassocephalum crepidioides) Menggunakan Metode Maserasi

2021 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Maria Christinela Domithesa ◽  
I Nengah Kencana Putra ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Design Method ◽  
Total Flavonoid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right ◽  
Range Test ◽  
Treatment Water

This study aims to determine the effect of various solvent towards antioxidant activity of kejompot (crassocephalum crepioides) extract and to know the right solvent to obtained extract with the highest antioxidant activity. This study used a Completely Randomized Design Method with 4 treatment (water, methanol 70%, ethanol 70%, acetone 70%,) using maseration method. The treatment was repeated four times to obtain 16 experimental units. If there was a significant effect on the observed parameters, then followed by Duncan multipple range test. The result showed that the treatment significantly effected (P<0.05) on each of parameters such as : yield, tanin total, flavonoid, vitamin C, antioxidant activity. This study showed that methanol 70% as the best treatment that obtained yield, tannin, flavonoid, Vitamin C and antioxidant activity respectively 18.76 %, 1.05 mg TAE/g, 7.59 mg QE/g, 8.68 mg AAE/g, and 103.1 ppm antioxidant activity based on IC50  .

scholarly journals Pengaruh Perbandingan Tepung Singkong Dan Puree Daun Kelor Terhadap Karakteristik Farfalle Basah

2021 ◽  
Vol 10 (1) ◽  
pp. 131
Author(s):  
I Putu Eka Putra Sentana ◽  
Putu Timur Ina ◽  
I Dewa Gede Mayu
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Water Content ◽  
Cassava Flour ◽  
Randomized Design ◽  
Total Antioxidant ◽  
Completely Randomized Design ◽  
Dark Green ◽  
The Right ◽  
Range Test

The purpose of this research were to know the comparison effect of cassava flour and puree of moringa leaves on the characteristics of wet farfalle and to know the right comparison on the best characteristics of wet farfalle.The experimental design used in this research was a completely randomized design (CRD) with comparison treatment of cassava flour and puree of moringa leaves consisting of 6 level of treatment namely 95%:5%; 90%:10%; 85%:15%; 80%:20%; 75%:25%; 70%:30%. All treatments were repeated three times so that there were obtained 18 experimental units. The data obtained were analiyzed by Analysis of Variance and if the treatment had significant effect then followed by Duncan Multiple Range Test. The result showed that the comparation of cassava flour and puree of moringa leaves had significant effect on the water content, vitamin C, chlorophyll total, antioxidant activity, color preference (scoring test) and overall acceptance (hedonic test) of wet farfalle. Comparison of 70 % cassava flour and 30 % puree of moringa leaves had the best characteristics with water content of 58.62%, vitamin C of 2.57 mg.AAE/g, chlorophyll total of 9.19 mg/100g, antioxidant activity of 72.90%, and value IC50 of 40,283 ppm, dark green color and rather liked, aroma rather liked, flavour, texture and overall acceptance liked. 

scholarly journals THE EFFECT OF POLYPROPYLENE THICKNESS AND TEMPERATURE ON THE QUALITY OF CAYENNE PEPPER DURING STORAGE

2020 ◽  
Vol 8 (10) ◽  
pp. 278-284
Author(s):  
Tri Luchi Proklamita ◽  
Aloysius Ng. Lende
Keyword(s):  
Weight Loss ◽  
Vitamin C ◽  
Moisture Content ◽  
Combination Treatment ◽  
Temperature Treatment ◽  
Storage Quality ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right

This study aims to determine the effect of different combinations of plastic thickness and temperature treatment combinations on the quality of cayenne pepper during storage, to determine the combination of plastic thickness and the right temperature to maintain the quality of cayenne pepper during storage. This study used a completely randomized design (CRD) with 9 treatments. The treatments tried in this study were thickness and temperature, namely Polypropylene 0.3 mm + tissue at 5°C, Polypropylene 0.3 mm + tissue at 10°C, Polypropylene 0.3 mm + tissue at 15°C, Polypropylene 0.4 mm + tissue at 5°C, Polypropylene 0.4 mm + tissue at 10°C, Polypropylene 0.4 mm + tissue at 15°C, Polypropylene 0.5 mm + tissue at 5°C, Polypropylene 0.5 mm + tissue at 10°C, Polypropylene 0.5 mm + tissue at 15°C. The treatment was repeated 3 times so that there were 27 experimental units. The results that showed the combination treatment of packaging thickness and temperature had a very significant effect on the storage quality of cayenne pepper on weight loss, vitamin C, texture, moisture content, and color, but had no significant effect on the color of the 8th day of the watershed. The combination treatment of plastic packaging thickness of 0.5 mm and temperature of 5°C can give the best effect on texture, vitamin C, moisture content, weight loss, and color, but at a thickness of 0.3 mm plastic and a temperature of 15°C does not give the best results on color 8th day.

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals ANALISIS PEMBERIAN VITAMIN C PADA ROTIFER DAN ARTEMIA TERHADAP SINTASAN, RASIO RNA/DNA, KECEPATAN METAMORFOSIS DAN KETAHANAN STRES LARVA RAJUNGAN (Portunus Pelagicus) STADIA ZOEA

2020 ◽  
Vol 2 (2) ◽  
pp. 30-34
Author(s):  
Faidar Faidar ◽  
Sutia Budi ◽  
Erni Indrawati
Keyword(s):  
Vitamin C ◽  
Stress Resistance ◽  
Brine Shrimp ◽  
Optimum Dose ◽  
Ratio Analysis ◽  
Chemical Physics ◽  
Portunus Pelagicus ◽  
Crab Larvae ◽  
Randomized Design ◽  
Completely Randomized Design

Penelitian ini bertujuan untuk menganalisis kandungan vitamin C rotifer, artemia dan larva rajungan setelah diperkaya dengan vitamin C dan menganalisis pemberian vitamin C terhadap sintasan, rasio RNA/DNA, kecepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea. Serta menentukan dosis optimum vitamin C yang menghasilkan sintasan, rasio RNA/DNA, ke-cepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea yang terbaik. Penelitian ini dilaksanakan pada bulan Mei sampai Juni 2020 di unit pembenihan kepiting dan rajungan Balai Perikanan Budidaya Air Payau (BPBAP) Takalar. Analisis Rasio RNA/DNA dilakukan di Laboratorium Uji Fisika Kimia BPBAP Takalar. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa pemberian vitamin C pada rotifer dan artemia dapat meningkatkan kandungan rotifer, artemia dan larva rajungan. Pemberian vitamin C pada rotifer dan artemia terhadap larva rajungan stadia zoea dapat meningkatkan sintasan, kecepatan metamorfosis dan ketahanan stres larva rajungan dan Dosis vitamin C 250 ppm yang terbaik pada pemeliharaan larva rajungan stadia zoea. This study aimed to analyze the content of vitamin C rotifer, artemia and small crab larvae after enriched with vitamin C and to analyze the provision of vitamin C to survival, RNA / DNA ratio, metamorphosis rate and stress resistance of crab larvae in zoea stage. Also, it aimed to determine the optimum dose of vitamin C which produces the best survival rate, RNA/DNA ratio, metamorphosis speed and stress resistance of crab larvae in zoea stage. This research was conducted from May to June 2020 in the hatchery unit of the Brackish Water Cultivation Fishery Center (BPBAP) Takalar. RNA / DNA ratio analysis was carried out at the Laboratory of Chemical Physics Testing of BPBAP Takalar. The research design used a completely randomized design (CRD). The results showed that giving vitamin C to rotifers and brine shrimp increased the content of rotifers, brine shrimp and crab larvae. Giving vitamin C to rotifers and artemia to larvae of zoea stadia crabs can increase survival, metamorphosis speed and stress resistance of crab larvae and the best dose of vitamin C 250 ppm in the maintenance of zoea stadia crab larvae.

scholarly journals PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Oreochromis Niloticus ◽  
Specific Growth ◽  
Good Combination ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Completely Randomized Design ◽  
Total Bacteria ◽  
Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase,  total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian  ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK MENIRAN (Phyllanthus niruri L.)

2020 ◽  
Vol 9 (4) ◽  
pp. 458
Author(s):  
Rizky Amalia Rachmawati ◽  
Ni Wayan Wisaniyasa ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Phyllanthus Niruri ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Effect On Yield ◽  
The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of gale of the wind extract and to find out the right type of solvent used to extract gale of the wind with the highest antioxidant activity using maceration method. The experimental design used in this study was a completely randomized design (CRD) with a type of solvent as a treatment. The treatment consisting of four levels, namely methanol, ethanol, acetone, isopropanol. All treatments were repeated four times so that they were obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. The results showed that the type of solvent treatment had a very significant effect on yield, total phenol, total flavonoids, total tannin, and antioxidant activity (IC50). The results showed that acetone solvent had the best treatment which produced a yield of 22.58%, total phenol content of 188.77 mg GAE/g, total flavonoid content of 247.60 mg QE/g, total tannin content of 297.51mg TAE/g, and antioxidant activity (IC50) of 15.19 ppm.

scholarly journals Produktivitas Rumput Hamil (Panicum maximum cv Hamil) yang Ditanam Menggunakan Benih Berbeda

2021 ◽  
Vol 26 (3) ◽  
pp. 444-449
Author(s):  
Ahmad Fanindi ◽  
Endang Sutedi ◽  
Harmini Harmini
Keyword(s):  
Reproductive Traits ◽  
Morphological Characters ◽  
Selection Method ◽  
Panicum Maximum ◽  
Plant Materials ◽  
Randomized Design ◽  
Different Types ◽  
Completely Randomized Design ◽  
The Difference ◽  
Generative Phase

Reproductive traits determine the selection method in plant breeding. The benggala grass of the Hamil cultivar was thought to be apomictic; thus, a study was conducted to determine its reproduction. The research began by studying the morphological characters, continued with observing the generative phase and seed production of the cultivar planted from seeds (generative) and from pols (vegetative). The experiment was conducted in the greenhouse of the Research Institute of Animal Production. The experimental design was a completely randomized design with ten replications, and the treatments were different types of plant materials: seeds and pols. The Hamil cultivar taken from RIAP Collection was planted in pots with a diameter of 40 cm and a height of 30 cm. The results showed that most of the morphological characters of the Hamil cultivar planted from seeds and from pols were not different (P >0.05), so it was presumed that they were apomictic. The difference in morphology was only in the length and width of the flag leaves and the length of the internodes, where the cultivars planted using seeds were higher. The production of seeds, pithy seed weight, and seed germination of cultivars planted using seeds were higher (P <0.05). Further research is needed to determine the apomixis properties based on cytologically Hamil cultivars.   Keywords: apomixis, benggala grass, morphology, plnting material; seed

scholarly journals PENGARUH PERBANDINGAN KENTANG KUKUS DAN TEPUNG KACANG HIJAU (Phaseolus radiatus L.) TERHADAP KARAKTERISTIK FLAKES

2019 ◽  
Vol 8 (1) ◽  
pp. 66
Author(s):  
Lina Nindyawati ◽  
Putu Timur Ina ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Protein Content ◽  
Water Content ◽  
Fat Content ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Green Bean ◽  
Bean Flour ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right

This study aims to determine the effect of steamed potatoes and green bean flour on the characteristics of flakes, and to know the right ratio of steamed potatoes and green bean flour which produced flakes with the best characteristics. The was used in this research completely randomized design with the treatment ratio of steamed potatoes and green bean flour, which consists of 6 levels such as: 35% : 65%, 40% : 60%, 45% : 55%, 50% : 50%, 55% : 45%, 60% : 40%. The treatment was repeated 3 times to obtained 18 units of the experiment. The data were analysed by variance analysis  and if the treatment had an effect on the variable then continued with Duncan test. The result showed steamed potatoes and green bean flour ratio had a significant effect on water content, ash content, fat content, protein content, carbohydrate content, color (hedonic), aroma (hedonic), texture (hedonic and scoring), taste (hedonic) and overall acceptance (hedonic). Ratio of 35% steamed potatoes and 65% green bean flour produced flakes with the best characteristics, with 2.41 % water content, 4.57 % ash content, 19.16% protein content, 10.20% fat content, 63.66% carbohydrate content, color liked, aroma liked, texture crunchy and liked, taste netral and overall acceptance rather liked.

Sign in / Sign up

Export Citation Format

Share Document