scholarly journals Optimization of polyphenols extraction process with antioxidant properties from wild Vaccinium myrtillus L. (bilberry) and Vaccinium vitis-idaea L. (lingonberry) leaves

2020 ◽  
Vol 3 (2) ◽  
pp. 149
Author(s):  
Radka Vrancheva ◽  
Ivan Ivanov ◽  
Ilian Badjakov ◽  
Ivayla Dincheva ◽  
Vasil Georgiev ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Vaccinium Myrtillus ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Polyphenol Compounds ◽  
Extraction Solvent ◽  
Ethanol Extracts ◽  
Vaccinium Myrtillus L

The aim of the current study was to optimize the extraction condition of polyphenol compounds with antioxidant properties from leaves of natural grown Vaccinium myrtillus L and Vaccinium vitis-idaea L. The extractions were carried out in ultrasonic bath at 40 ºC for 20 minutes with different solvents (water, 20 % ethanol, 40 % ethanol, 60 % ethanol, 80 % ethanol and 96 % ethanol) and different hydro module of samples and solvents used (1:50, 1:100 and 1:200). The highest total phenol content in the leaves of V. myrtillus L. and V. vitis-idaea L. was found when 40% ethanol extract was used (90.50 ± 0.05 mg GAE/g DW and 96.68 ± 0.05 mg GAE/g DW, respectively). The highest total flavonoid content of the leaves of both species was obtained with 80 % ethanol as extraction solvent. The highest level of total proanthocyanidins were in the 60 % ethanol extract of V. myrtillus L. and in the 80 % ethanol extract of V. vitis-idaea L (13.12 ± 0.11 mg LE/g DW and 24.22 ± 0.21 mg LE/g DW, respectively). The highest ability to scavenge DPPH radicals possessed the 40 % ethanol extracts from the leaves of both species (693.99 ± 4.05 mM TE/g DW for V. myrtillus L. and 1083.18 ± 8.48 mM TE/g DW for V. vitis-idaea L). Data analysis showed that the maximal amount of polyphenols was extracted at a hydro-module of 1: 100. HPLC analysis revealed that the dominant phenolic acid in the leaves of V. myrtillus L was chlorogenic acid (13.45 mg/g DW), while ferulic acid (49.48 mg/g DW) was present at the highest concentration in the leaves of V. vitis-idaea L.

scholarly journals Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

2020 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Shreya Udaya ◽  
Nishith Babu ◽  
Dechamma Pandyanda Nanjappa ◽  
Krithika Kalladka ◽  
Gunimala Chakraborty ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Stem Bark ◽  
Oxygen Species ◽  
Cassia Fistula ◽  
Potential Applications ◽  
Ethanol Extracts ◽  
Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 142 ◽  
Author(s):  
Chloé Placines ◽  
Viana Castañeda-Loaiza ◽  
Maria João Rodrigues ◽  
Catarina G. Pereira ◽  
Azzurra Stefanucci ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
High Performance ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Docking Studies ◽  
In Silico Studies ◽  
Cakile Maritima ◽  
Southern Portugal ◽  
Kaempferol Glycosides ◽  
Ethanol Extracts

Cakile maritima Scop. (sea rocket) is an edible halophyte plant with several ethnomedicinal uses. This work reports the chemical profile and bioactivities of food grade extracts from sea rocket organs. Toxicity was determined on mammalian cells, and phenolic profiling and the quantitation of the main metabolites were made by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Enzymatic inhibition was determined towards acetyl- and butyrylcholinesterase (AChE, BuChE), α-glucosidase, α-amylase, and tyrosinase. Docking studies were performed to tyrosinase, on the major metabolites, and samples were tested for antioxidant properties. Extracts were not toxic, were constituted mainly by flavonoids, and some compounds (roseoside and oleuropein) are here described for the first time in the species. The aerial organs’ ethanol extract had relevant activity towards 2,2-diphenyl-1-picrylhydrazyl [DPPH, half maximal inhibitory concentration (IC50) = 0.59 mg/mL], and ferric-reducing activity power (FRAP, IC50 = 0.99 mg/mL). All samples were more active towards AChE than on BuChE. The ethanol fruits’ extract inhibited α-glucosidase [2.19 mmol of equivalent of acarbose (ACAE)/g]. Samples were active against tyrosinase, especially the aerial organs’ ethanol extracts [25.9 mg of equivalent of kojic acid (KAE)/g]. Quercetin and kaempferol glycosides fit well into the enzymatic pocket of tyrosinase. Our results suggest sea rocket as a candidate to be further explored as a source of bioactive products.

scholarly journals Isolation of Betulinic acid from the stem bark and root of Uapaca guineensis

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Abdulrazaq Ogunmoye
Keyword(s):  
Betulinic Acid ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Stem Bark ◽  
Evergreen Forest ◽  
Spectra Analysis ◽  
Pentacyclic Triterpenoid ◽  
Ethanol Extracts ◽  
Isolated Compound

Uapaca guineensis Mull. Arg. (Euphorbiaceae) commonly called false mahogany and “Ewe akun” in the western part of Nigeria grows in humid localities, mixed evergreen forest and sea-level up to 1100 m altitude. The study was designed to isolate Betulinic acid (3?-hydroxylup-20-(29)-en-28-oic acid) which is a naturally occurring pentacyclic triterpenoid that exhibited antibacterial, antimalarial, anti-inflammatory, anthelmintic and antioxidant properties from the stem bark and root of Uapaca guineensis. The air-dried and pulverized stem bark and root of U. guineensis (0.5 kg each) were separately extracted with aqueous ethanol (80%) for 72 hour at room temperature, filtered, concentrated on rotatory evaporator at 37 oC and then stored in a desiccator. The ethanol extracts of the stem bark was partitioned successively with n-hexane, dichloromethane and methanol, while ethanol extract of the root was chromatographed directly. The dichloromethane and methanol extracts of the stem bark was also chromatographed. These results in the formation of whitish powder that are UV inactive and also gave single spot each with cerium sulphate. They are coded as DBG-D13 (25.0 mg), MBG-Q3 (6.1 mg) and ERG-E7 (20.0 mg) for dichloromethane, Methanol and Ethanol extracts respectively. The structure was unequivocally established by 1HNMR, 13CNMR, 2D-NMR, IR, UV and MS spectra analysis as well as by comparison of the spectra data with previously reported values. The dichloromethane, methanol and the ethanol extracts all yielded betulinic acid in substantial quantity. The isolated compound is a confirmation to existing information and an addition to the library of organic compounds.

scholarly journals Antioxidant properties of Rubus discolor leaf extracts

2016 ◽  
pp. 189-196 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Petar Marin
Keyword(s):  
Antioxidant Properties ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Aqueous Extracts ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Dry Extract

In this work were examined aqueous, methanol, ethanol and acetone leaf extracts of Rubus discolor, wild growing blackberry, for their antioxidant properties and total phenol and flavonoid content. The total phenol content (TPC) varried from 250.05 to 446.61 mg GAE/g of dry extract, while total flavonoid content (TFC) was in range between 22.44 and 61.15 mg QE/g of dry extract. Aqueous extracts were the richest in phenols, as well as in flavonoids. In vitro antioxidant capacity of leaf extracts was evaluated by 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging procedures and ferric reducing ability of plasma (FRAP) assay. Aqueous extracts were the most effective through all antioxidant tests. The total phenol content highly correlated with antioxidant activity of extracts. Moreover, weak correlation was established between total phenol and total flavonoid content. The results presented in this work indicate that phenol compounds contribute to antioxidant ability of extracts.

scholarly journals Correlation between total flavonoid content and total phenolic content on antioxidant activity of ethanol extracts from three cultivars of papaya leaves

2020 ◽  
Vol 11 (2) ◽  
pp. 1883-1887
Author(s):  
Prakit Chaithada ◽  
Praweena Whenngean ◽  
Radchada Fungfueng ◽  
Saowanee Maungchanburee
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ethanol Extracts ◽  
Dpph Radical Scavenging

The aim of this study was to elucidate the correlation between total flavonoid content and total phenolic content on antioxidants activity of ethanol extracts from three cultivars of papaya leaves: 'Holland,' 'Khak Dam' and 'Red Lady.' All crude extracts were investigated to find antioxidant capacity in DPPH radical scavenging. The result indicated that the ethanol extract of ‘Red Lady’ papaya leaves exhibited the highest level of DPPH radical scavenging activity with the IC50 of 0.18 mg/mL, followed by the ‘Khak Dam’ and ‘Holland’ papaya leaves having an IC50 value of 0.24 and 0.44 mg/mL, respectively. The ethanol extract from 'Red Lady' papaya leaves showed the highest level of total flavonoid content (TFC) of 276.72 ± 1.04 µgQE/g DW and total phenolic content (TPC) of 169.85 ± 6.54 mgGAE/g DW. All three cultivars showed a distinctive correlation between IC50 and the content of both total flavonoid and total phenolic with a negative relationship of Pearson’s correlation of -0.922 and -0.940, respectively.

scholarly journals Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

2010 ◽  
Vol 46 (4) ◽  
pp. 715-722 ◽  
Author(s):  
Cláudia Alexandra de Andrade ◽  
João Luiz de Souza Carvalho ◽  
Miriam Machado Cunico ◽  
Ana Luísa Lacava Lordello ◽  
Carmen Etsuko Kataoka Higaskino ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Ethyl Acetate Fraction ◽  
Total Phenol Content

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL-1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL-1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL-1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.

scholarly journals Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

2014 ◽  
Vol 22 (3) ◽  
pp. 296-302 ◽  
Author(s):  
Quy Diem Do ◽  
Artik Elisa Angkawijaya ◽  
Phuong Lan Tran-Nguyen ◽  
Lien Huong Huynh ◽  
Felycia Edi Soetaredjo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Extraction Solvent

scholarly journals COMPARATIVE STUDY OF THE INFLUENCE OF ULTRASONIC INFLUENCES ON THE EXTRACTION OF ANTI-OXIDANT COMPOUNDS OF BLACKBERRY BERRIES (VACCINIUM MYRTILLUS L.)

2020 ◽  
pp. 167-177
Author(s):  
Nadezhda Viktorovna Makarova ◽  
Natal'ya Borisovna Yeremeyeva
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Vaccinium Myrtillus ◽  
Ultrasonic Extraction ◽  
Biologically Active ◽  
Extraction Technology ◽  
High Biological Activity ◽  
Plant Materials ◽  
Dpph Method ◽  
Vaccinium Myrtillus L

The interest in berries from food manufacturers is huge, which is explained by good organoleptic characteristics and high biological activity of berries. One way to preserve the properties of berries throughout the year is to obtain extracts. Determining the conditions and type of optimal extraction technology to obtain biologically active substances from plant materials is an important stage in the production of new natural preventive additives. The aim of this work is to study the chemical composition and antioxidant activity of blueberry extracts (Vaccinium myrtillus L.) obtained by various methods: maceration, ultrasonic and microwave extraction. The use of ultrasonic extraction as a method of intensifying the process of extracting biologically active compounds from blueberries is very effective. This proves an almost 2-fold increase in the flavonoid content in the extracts. However, the content of anthocyanins in the extracts of blueberries is not the highest for ultrasonic extraction (a decrease by 3.3 times compared with maceration), in all likelihood due to the instability of this class of compounds in this type of processing. Among the extracts of blueberries obtained by three technologies (maceration, microwave and ultrasound treatment), it is the ultrasound extract that has the highest antioxidant activity (9.5±0.1 mg/cm3, 18.18 mmol±0.24 Fe2+/1 kg, 58.6%), determined by three methods: DPPH method, FRAP method, method for assessing antioxidant properties using a model system with linoleic acid. Ultrasonic extraction was chosen as a technology for obtaining a concentrated blueberry extract.

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