scholarly journals Pengaruh Pemberian Ekstrak Alga Merah (gracilaria verrucosa) terhadap Aktivitas SGPT pada Tikus Putih (rattus norvegicus) Jantan Galur Wistar yang Diinduksi Parasetamol Dosis Tinggi

2018 ◽  
Vol 7 (1) ◽  
pp. 75
Author(s):  
Akbar Maharudin Fikri ◽  
Sihning EJ Tehupuring
Keyword(s):  
Red Algae ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Gracilaria Verrucosa ◽  
High Dose ◽  
Test Results ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
High Doses

Background: Administration of high dose paracetamol may increase the metabolic pathways that produce N-acetyl-p-benzoquinonimin (NAPQI). NAPQI is a reactive substance, resulting liver tissue damage and increase SGPT activity. Red algae extract contains flavonoids that act as antioxidants.Objectives: This research aimed to determine the effect of red algae (Gracilaria verrucosa) extract to the activity of serum glutamic pyruvic transaminase (SGPT) in male Wistar rats (Rattus norvegicus) induced by high dose paracetamol.Methodology: This research used 24 animal of Male Wistar rats. These rats were divided into 3 groups: 1) group of rats fed with standard food; 2) group of rats given paracetamol 1750 mg/kg on day 10; and 3) group of rats given red algae extract 200 mg/kg/day on day 1-11 and paracetamol 1750mg/kg on day 10. On day 11, all rats were sacrificed and SGPT activities were measured by spectrophotometry method.Result: Mann-Whitney test results showed significant difference (p = 0.021) between SGPT activity of group of rats fed with standard food (=81.95±10.103 U/L) and group of rats induced by high dose paracetamol (=97.06±11.19 U/L). Furthermore, there was significant difference (p = 0.016) between SGPT activity of group of rats induced by high dose paracetamol (=97.06±11.19 U/L) and group of rats induced by high doses of paracetamol and fed with red algae extract (=82.73±11.233 U/L).Conclusion: The conclusion of this research showed that high dose paracetamol significantly increased SGPT activity and red algae extract significantly decreased SGPT activity because it contains flavonoids that act as antioxidants.

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (9) ◽  
pp. 879-882
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

scholarly journals THE EFFECT OF SINGLE- AND REPEATED- EXCESSIVE SWIMMING EXERCISE ON KIDNEY HISTOPATHOLOGY OF MALE RATS WISTAR STRAIN (Rattus norvegicus)

2020 ◽  
Vol 1 (1) ◽  
pp. 33
Author(s):  
Wenni Juniarni Tripani ◽  
Muhammad In’am Ilmiawan ◽  
Willy Handoko
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Exercise Group ◽  
Proximal Tubules ◽  
Swimming Exercise ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Mean

Background: Physical exercise aims to improve or maintain physical fitness. However, excessive physical exercise may cause increase of oxidative stress which leads to cellular injury, including in the proximal tubules of kidney. This research aims to find out the effect of single- and repeated- excessive swimming exercise to the kidney histopathology of male wistar rats (Rattus norvegicus). Methods: This research was an experimental study with complete random and post test only control group design. Twenty seven male wistar rats were divided into three treatment groups: control group, single- excessive swimming exercise group (45 minutes for one day), and repeated- excessive swimming exercise group (45 minutes/day for seven days). In the end of the treatment, the kidney of the rats were taken to be analyzed by using hematoxylin-eosin stain. The mean of proximal tubules injury of kidneys were counted by two observers using blinded method. Data were analyzed by using one way anova test. Result: The analysis showed there was a significant difference in the mean percentage of proximal tubules injury between control group and single swimming group as well as the repeated swimming group (Anova p=0,000), also there was significant difference between the treatment groups (p=0,020). The highest average of proximal tubules injury was in the single swimming group. Conclusion: Single- and repeated- excessive swimming exercise causes the proximal tubules injury of kidney.

scholarly journals Perbedaan efek pemberian tunggal vitamin E dengan pemberian kombinasi vitamin e dan zink terhadap kualitas spermatozoa tikus wistar (Rattus Norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Iis A.H. Silalahi ◽  
Grace L.A. Turalaki ◽  
Janette M. Rumbajan
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Enzymatic Antioxidant ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Smoking can increase free radicals in sperm, which can damage sperm. Vitamin E as non-enzymatic antioxidant and zinc as enzymatic antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin E and zinc on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. The research used a completely randomized experimental design. Samples were 9 male wistar rats (Rattus norvegicus) which were randomly divided into 3 groups. Each group was given exposure to cigarette smoke from 2 bars of cigarette/day, which treatment group (P1) also was given vitamin E 1,44 mg/day and treatment group (P2) also was given combination of vitamin E 1,44 mg/day and zinc 10 mg/day. The treatment was given for 50 days. The results of this research showed a significant difference in the motility and morphology of spermatozoa between the treatment group (P1) which received exposure the cigarette smoke and vitamin E and treatment group (P2) which received exposure to cigarette smoke and combination of vitamin E and zinc (p<0,05) compared with the group which received exposure only cigarette smoke without vitamin E or zinc. The motility of spermatozoa treatment group (P2) which received exposure the cigarette smoke and combination vitamin E and zinc was found significant difference with the treatment group (P1) which received exposure the cigarette smoke and vitamin E (p <0,05). Conclusion: The conclusion from this research showed that there is difference between spermatozoa quality especially motility and morphology of group treated with vitamin E and combination of vitamin E and zinc after exposure to cigarette smoke, with combination of vitamin E and zinc had an higher average on spermatozoa quality especially motility.Keywords: vitamin E, zinc, cigarette smoke, quality of spermatozoa Abstrak: Merokok dapat menyebabkan peningkatan radikal bebas yang dapat merusak sperma. Vitamin E sebagai antioksidan non enzimatis dan zink sebagai antioksidan enzimatis merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin E dan kombinasi vitamin E dengan zink terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak legkap. Sampel yang digunakan adalah 9 tikus wistar (Rattus norvegicus) jantan yang dibagi secara acak menjadi 3 kelompok. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi vitamin E 1,44 mg/hari, dan kelompok perlakuan (P2) diberi kombinasi vitamin E 1,44 mg/hari dan zink 10 mg/hari. Perlakuan diberikan selama 50 hari. Hasil penelitian menunjukkan adanya perbedaan signifikan pada motilitas dan morfologi spermatozoa kelompok perlakuan (P1) dengan perlakuan paparan asap rokok dan vitamin E dan kelompok perlakuan (P2) dengan perlakuan paparan asap rokok dan kombinasi vitamin E dengan zink dibandingkan dengan kelompok control (P0) yang hanya mendapat paparan asap rokok (p<0,05). Motilitas spermatozoa kelompok perlakuan (P2) didapati hasil yang berbeda signifikan dibandingkan dengan kelompok perlakuan (P1) (p<0,05). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan signifikan kualitas spermatozoa yaitu pada motilitas dan morfologi antara pemberian tunggal vitamin E dan kombinasi vitamin E dengan zink setelah pemaparan asap rokok, dengan kombinasi vitamin E dan zink memiliki rata-rata kualitas spermatozoa lebih tinggi khususnya motilitas.Kata kunci: vitamin E, zink, asap rokok, kualitas spermatozoa

scholarly journals Pengaruh Pemberian Ekstrak Daun Mahkota Dewa pada Gambaran Histopatologi Paru Tikus Putih yang Dipapar Asap Rokok

2019 ◽  
Vol 17 (1) ◽  
pp. 48
Author(s):  
NGURAH BAGUS RADITYA SRI DAMAR ◽  
JANTO POERNOMO HADI ◽  
EDIJONO EDIJONO ◽  
TROEF SOEMARNO
Keyword(s):  
Inflammatory Response ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
White Male ◽  
Significant Difference ◽  
Lung Histopathology ◽  
Male Wistar Rats ◽  
Phaleria Macrocarpa

<p><strong>Background</strong>: Smoking is a bad habit for health. There are many dangerous components inside a cigarette. The smoke that comes out from the cigarette contains many harmful components. Smoking and getting exposed to cigarette smoke in a long period of time can cause inflammatory response on respiratory tract. Mahkota dewa leaf contains beneficial biological component, such as <em>flavonoid</em>, <em>alkaloid</em>, and<em>saponin </em>that have anti-inflammatory, anti-cancer, and many others.</p><p><strong>Aim :</strong>The purpose of this research was to determine the effect of administration of mahkota dewa leaf (<em>Phaleria macrocarpa</em>) extract onlung histopathology in white male Wistar rats (<em>Rattus norvegicus</em>) exposed to cigarette smoke.</p><p><strong>Methods : </strong>This research is a true laboratory experimental research using <em>Post-Test Only Control Group Design</em>. The subject in this research were 30 male Wistar Rats (<em>Rattus norvegicus</em>) which were divided into 4 groups, a group without given any treatment (K-), a group where they only getting exposed to cigarette smoke (K+), a group where they exposed to cigarette smoke and given mahkota dewa leaf extract at 1500 mg/kgBW/day dose (Eg1), and the group exposed to cigarette smoke and a mahkota dewa leaf extract at a 2500 mg/kgBW/day dose (Eg2).</p><p><strong>Result            : </strong>1.) There was a significant difference on lung histopathology between K- and K+, Eg1, and Eg2. 2.) There was no significant difference on lung histopathology between K+, Eg1 and Eg2.</p><p><strong>Conclusion : </strong>Exposure to 5 cigarette smoke in 21 days caused lung histopathology changes. However, there was no effect of the administration of mahkota dewa leaves extract at 1500 mg/kgBW/day and 2500 mg/kgBW/day on lung histopathology of white male Wistar rats exposed to 5 cigarette smoke in 21 days.</p><p><strong>Keyword : </strong><em>Phaleria macrocarpa leaf, </em>Flavonoid, Alkaloid, cigarette smoke, inflammatory response, Infiltration of Inflammatory cells.</p>

scholarly journals Pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pretty P. Makasenda ◽  
Janette M. Rumbajan ◽  
Grace L.A. Turalaki
Keyword(s):  
Reactive Oxygen Species ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Cigarettes are composed of hazardous chemicals such as nicotine, tar and carbon monoxide (CO) to name a few. In just a single puff of a cigarette, there are 1014 free radical molecules also known as Reactive Oxygen Species (ROS) that can cause sperm damage. Various natural ingredients native to Indonesia were found to contain various antioxidants, one of them is honey. The effects of honey as antioxidant may protect body cells in neutralizing free radicals caused by smoking and reducing the damage to spermatozoa cell that is caused by ROS and thereby avoiding the declining quality of spermatozoa. This study aimed to determine the effect of honey on the quality of spermatozoa of Wistar rats (Rattus norvegicus) that had been exposed to cigarette smoke. Subjects of this study were nine male Wistar rats (Rattus norvegicus) randomly divided into three groups weighing 150-200 g with the age range of 12-14 weeks. Each group of mice was given exposure to the smoke of 2 cigarettes a day in which the treatment group (P1) is also given 0.5 ml of honey per day, and the treatment group (P2) honey 1 ml / day. The results showed that honey treatment can improve concentration, motility, and morphology of spermatozoa Wistar rats (Rattus norvegicus) by exposure to cigarrets smoke. Occurred a significant difference of concentration, motility, and morphology of spermatozoa group given only the exposure to cigarette smoke and the group given exposure to cigarette smoke and honey. This results showed that 1 ml of honey per day could improve the quality of spermatozoa.Keywords: honey, cigarrets smoke, spermatozoa Abstrak: Rokok mengandung bahan kimia yang berbahaya, yaitu nikotin, tar dan gas karbon monoksida (CO). Dalam satu kali hisapan rokok terdapat 1014 molekul radikal bebas atau Reactive Oxygen Species (ROS) yang dapat merusak spermatozoa. Berbagai bahan alam asli Indonesia banyak mengandung antioksidan, salah satunya pada madu. Efek madu sebagai antioksidan dapat melindungi sel-sel tubuh termasuk menetralisir radikal bebas yang disebabkan oleh rokok dan mengurangi kerusakan sel spermatozoa yang disebabkan oleh ROS sehingga menghindari menurunnya kualitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Subjek penelitian ini menggunakan 9 ekor tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok secara acak dengan berat badan 150-200 gram dan berumur 12-14 minggu. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi madu 0.5 ml / hari, dan kelompok perlakuan (P2) diberi madu 1 ml / hari. Hasil penelitian memperlihatkan bahwa pemberian madu dapat meningkatkan konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Terjadi perbedaan yang bermakna dari konsentrasi, motilitas, dan morfologi spermatozoa kelompok yang hanya diberi paparan asap rokok dan kelompok yang diberi paparan asap rokok dan madu. Hasil tersebut menunjukan bahwa madu 1 ml / hari dapat meningkatkan kualitas spermatozoa. Kata kunci: madu, asap rokok, spermatozoa

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (4) ◽  
pp. 851-854
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

Pengaruh Pemberian Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak

2020 ◽  
Vol 17 (2) ◽  
pp. 192
Author(s):  
RONALDO LAU ◽  
SULISTIANA PRABOWO ◽  
RIAMI RIAMI
Keyword(s):  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
White Male ◽  
Standard Diet ◽  
High Fat ◽  
Caulerpa Racemosa ◽  
Significant Difference ◽  
Male Wistar Rats

<p align="justify"><strong>ABSTRACT</strong><strong></strong></p><p align="justify"><strong>Background</strong>: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (<em>Caulerpa racemosa</em>) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract  on decreasing LDL cholesterol of white male Wistar rats (<em>Rattus norvegicus</em>) fed with high fat diet.</p><p align="justify"><strong>Method</strong>:  24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15<sup>th</sup>-28<sup>th</sup> days. Then the blood LDL cholesterol level measured on the 29<sup>th</sup> day.</p><p align="justify"><strong>Result :</strong> One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).</p><p align="justify"><strong>Conclusion: </strong>high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (<em>Caulerpa racemosa</em>) significantly decrease blood LDL cholesterol level.</p><p align="justify"> </p><p align="justify"><strong>Keywords :</strong>Sea grapes extract, LDL cholesterol, high fat diet</p>

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

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