Influence of Trichoderma inoculation on four crucial defense-related enzymes and leaf soluble protein level of poplar

2019 ◽  
Vol 52 (3) ◽  
Author(s):  
Yue-Feng Wang ◽  
Abdul Majeed Baloch ◽  
Jun-Jie Deng ◽  
Abdul Wahid Baloch ◽  
Xue-Yue Hou ◽  
...  
2014 ◽  
Vol 6 (1) ◽  
pp. 31-37 ◽  
Author(s):  
V. Rajagopal ◽  
H.L. Ramesh ◽  
V.N. Yogananda Murthy ◽  
K.N. Ninge Gowda

Silkworm Bombyx mori L. is prone to be attacked by pathogen and more notably Beauveria bassiana L. Bed disinfectant Kavach was inoculated in different dosages (0.2%-1.6%) to IV and V instar silkworms of both bivoltine and cross breeds. Potency of disinfectant was assessed for the parameters such as survivability, larval duration, physiological, chemo and bio-assay tests. It was revealed that, bivoltine (NB4D2) silkworms were highly susceptible to diseases compared to cross breeds (PMxNB4D2). In bivoltine silkworms, survivability was found to be 61.15% at 1.6% of Kavach, when dusted twice during IV and V instar and crossbreed silkworms exhibited better resistance of 63.10% with the same treatments. Kavach treated silkworms showed decreased larval duration compared to control worms. Crossbreed silkworms were capable of maintaining high level of soluble proteins in spite of infection on 3rd day (17.40%), 4th day (20.50%) and 5th day (21.55%) whereas in bivoltine silkworms soluble protein level was brought down on 3rd day (19.30%), 4th day (22.40%) and 5th day (23.40%). Total soluble sugarsvaried from third day till fifth day in both the races. Kavach dusted twice at 1% proved to be very useful in the improvement of various commercial cocoons characters.


2009 ◽  
Vol 33 (2) ◽  
pp. 315-324 ◽  
Author(s):  
Andre Rodrigues Reis ◽  
José Laércio Favarin ◽  
Luiz Antônio Gallo ◽  
Eurípedes Malavolta ◽  
Milton Ferreira Moraes ◽  
...  

Nitrate reductase is the first enzyme in the pathway of nitrate reduction by plants, followed by glutamine synthetase, which incorporates ammonia to glutamine. The purpose of this study was to evaluate the nitrate reductase and glutamine synthetase activity, total soluble protein content, N and Ni content in coffee leaves during fruit development under field conditions to establish new informations to help assess the N nutritional status and fertilizer management. The experimental design was in randomized complete blocks, arranged in a 3 x 6 factorial design, with five replications. The treatments consisted of 3 N rates (0 - control, 150 and 300 kg ha-1) and six evaluation periods (January, February, March, April, May, and June) in six-year-old coffee (Coffea arabica L.) plants of Catuaí Vermelho IAC 44 cv. The nitrate reductase and glutamine synthetase activities, leaf soluble protein, and N concentrations increased linearly with the N rates. During fruit development, the enzyme activity, leaf soluble protein and N content decreased, due to the leaf senescence process caused by nutrient mobilization to other organs, e.g, to the berries. Leaf Ni increased during fruit development. Beans and raisin-fruits of plants well-supplied with N had higher Ni contents. Enzyme activities, total leaf N and leaf soluble protein, evaluated during the green fruit stage in March, were significantly correlated with coffee yield. These variables can therefore be useful for an early assessment of the coffee N nutritional status as well as coffee yield and N fertilization management.


1969 ◽  
Vol 47 (7) ◽  
pp. 1051-1054 ◽  
Author(s):  
William Habig ◽  
David Racusen

The level of certain enzymes was compared in albino, etiolated, and normal corn leaves. It was found that malic dehydrogenase and peroxidase were highest in light-grown albinos. Ribulose-1,5-diphosphate carboxylase, considered a chloroplast enzyme, was present in increasing amounts in the order albino < etiolated green < green. The level of this enzyme corresponded to the degree of chloroplast ultrastructure as determined by electron microscopic examination and insoluble protein. Only light-grown normal leaves had high catalase activity. All plants responded to light by an increased level of soluble protein, but only normal (i.e. non-albino) leaves showed an increase in insoluble protein. At least part of the higher soluble protein level could be accounted for by an electrophoretically distinct protein found only in light-grown plants.


2020 ◽  
Vol 4 (2) ◽  
pp. 106
Author(s):  
Wahyu Vera Wardani

Saponin in black tea is predicted having potential to reduce formaldehyde level in preserved fresh tuna (Auxis thazard) using formaldehyde. This research used experimental design, post-test only control group. Variables examined were formaldehyde level, organoleptic values (eye appearance, gill appearance, color, smell, and texture), and soluble protein level. Formaldehyde level was analyzed using Nash reagent by spectrophotometric method. Organoleptic value was conducted using questionnaire by 16 panelists. Soluble protein level was analyzed using Lowry reagent by spectrophotometry. Result showed significant difference between formaldehyde level in control and treatment gorup, but did not significantly difference between formaldehyde level that was immersed in water and black tea solution. Soluble protein level showed no significant difference between samples. There were significant differences in appearance and eye color, while there were no differences in gill appearance, smell and texture. The best treatment was samples immersed in black tea 1,5% for 90 minutes with average levels of formaldehyde 55,76 ug/g, soluble protein content of 0,042 mg/g, eyes appearance 1,59 (slightly white orange), the appearance of gills 2,41 (less crimson), 2,78 body color appearance (slightly orange white), the smell of 2,63 (pungent), the texture of 1,69 (slightly soft). Keyword : black tea, formaldehyde, saponin


HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 539e-539
Author(s):  
Bruce D. Kelley ◽  
J. Scott Cameron

Several anatomical and physiological parameters were measured in 32 genotypes of fragaia, including the cultivated strawberry (frapria × ananassa) and its progenitor speck F. chiloensis and F. virginiana Measurements were made using potted. runner-propagated, &month-old greenhouse-grown plants growing under long day (14/10) conditions. Significant differences in CO2 assimilation rates (leaf area and dry weight bases). leaf chlorophyll content, leaf soluble protein content, and leaf anatomy were found between subspecies of F. virginana as well as among species. Recessed stomata and greater water use efficiency were observed in F. virginana ssp glauca as well as in F. chiloensis genotypes. In addition, it appears that leaf anatomy characteristics, as studied using light microscopy, may he useful in corroborating taxonomic decisions based upon gross morphology in Fragaria.


Agriculture ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 207
Author(s):  
Jie Kuai ◽  
Xiaoyong Li ◽  
Yan Xie ◽  
Zhen Li ◽  
Bo Wang ◽  
...  

Four nitrogen rates (0, 90, 180, and 270 kg ha−1) were applied to the waterlogging-tolerant variety ZS 9 and the sensitive variety GH01. Seedlings with five leaves were waterlogged for 0 (control) or 10 days to investigate the effects of nitrogen on the quality of waterlogged rapeseed. Compared with controls, the seed oil content of waterlogged rapeseed increased slightly in GH01 and significantly in ZS 9 with nitrogen application, which can be explained by the following. (1) after waterlogging, the biomass distribution in roots and leaves of ZS 9 decreased, which alleviated physiological water shortage. Conversely, biomass distribution in roots of GH01 increased, which was not synchronized with the leaf biomass change. (2) After waterlogging at 90–270 kg N ha−1, the leaf number at bolting and flowering was increased in ZS 9 but decreased in GH01 compared with the control. The decrease in leaf area and SPAD value were greater for GH01 after waterlogging, which limited photosynthesis. (3) The leaf soluble protein at bolting was highest in ZS 9 and lowest in GH01. The sensitive variety showed poor growth. The inhibition of seed protein synthesis resulted in an increase in the oil content of waterlogged rapeseed with nitrogen. The seed oil of the waterlogging-tolerant variety was most significantly negatively correlated with leaf soluble protein content at the flowering stage, while the protein content showed the opposite correlation. The seed oil of the waterlogging-sensitive variety was most significantly negatively correlated with the number of leaves at the bolting and flowering stage, while the seed protein content had opposite correlations.


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