〈1048〉 Quality of Biotechnological Products: Analysis of the Expression Construct in Cells Used for Production of r-DNA Derived Protein Products

2021 ◽  
Keyword(s):  
Biotechnological Products ◽  
Expression Construct ◽  
Products Analysis ◽  
In Cells

scholarly journals Influence of the origin on selected determinants of the quality of pork meat products

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 547-552 ◽  
Author(s):  
B. Garbowska ◽  
M. Radzymińska ◽  
D. Jakubowska
Keyword(s):  
Glutamic Acid ◽  
Protein Content ◽  
Acid Content ◽  
Salt Content ◽  
Meat Products ◽  
Fat Content ◽  
Pork Meat ◽  
Significant Difference ◽  
Products Analysis

in recent years, great attention has been paid to the quality of eaten meat and its products. There have been launched a lot of promotional campaigns aimed at providing opportunities for the consumption of traditional products. Based on the experiment, a significantly higher protein content was found in sausages produced by large producers (24.73 ± 1.98%). The fat content was significantly higher in traditional ham (16.25 ± 14.47%), compared with local ham (4.38 ± 2.26%) and the mass (9.29 ± 5.25%). The samples of traditional and local ham had a significantly higher salt content (3.31 ± 0.72 and 2.90 ± 0.54%, respectively). No dye compounds were detected in any of the tested samples. There were no statistically significant differences in hydroxyproline and l‑glutamic acid content between traditional and conventional samples of meat products. Analysis of nitrate (V and III) showed a statistically significant difference in the average contents of these compounds. Significantly higher levels of nitrates were revealed only in traditional ham samples (12.60 ± 8.08 mg NaNO(V)/kg and 17.53 ± 27.91 mg NaNO(III)/kg of the product, respectively), wherein there was a large variation in the content of these compounds in the samples.

The Influence of the Weld Line and the Selection of Mold Gate Location

2016 ◽  
Vol 693 ◽  
pp. 952-958
Author(s):  
Guo Yi Guo
Keyword(s):  
Injection Molding ◽  
Great Influence ◽  
Weld Line ◽  
Simulation Software ◽  
Location Selection ◽  
Gate Location ◽  
Products Analysis ◽  
Important Position ◽  
The Impact

Through injection molding simulation software and examples of injection,This paper analyzes the causes of the weld line when injection molding products, the impact on the quality of products, analysis of the injection mold gate location selection problems and defects produced by principle, think weld line has a great influence on the quality of injection molding, on this basis, put forward in view of the weld line, the influence of the mold gate location selection method in the practical work of is: reasonable choose the location of the gate, to control the degree of weld line, as far as possible to reduce the number of the weld line, or make them produce not important position in the mold. Etc.

Evolution of notion "quality of cartographic work"

2017 ◽  
Vol 922 (4) ◽  
pp. 13-18
Author(s):  
I.I. Lonskij ◽  
I.A. Zaharenko ◽  
V.V. Shlapak ◽  
K.S. Perevoznikov ◽  
K.S. Lotova
Keyword(s):  
Information Content ◽  
Spatial Data ◽  
Systems Approach ◽  
Geometric Accuracy ◽  
Geospatial Information ◽  
Information Product ◽  
Analysis And Evaluation ◽  
Products Analysis ◽  
Mathematical Foundations

The evolution of the approach to understanding the quality of the map, from the authenticity, completeness, details of cartographic images and accuracy to using complex systems approach to the criteria for assessing the quality of cartographic products and use of such concepts as the descriptiveness, usefulness and value is considered at the article. The proposed approach of the authors is based upon the analysis and evaluation of the map’s mathematical foundations, the completeness of the maps’ content, authenticity, the geometric accuracy, modernity, the quality of design, scientific and practical value. The requirements for maps’ images, the principles of the cartographic products’ analysis, a set of properties of interest to the consumer were reviewed. The basic properties characterizing the evaluation of the quality of the map were systematized. It is noted that the card is an information product carrier of geospatial information, which displays spatial data. In this context, it is also advisable to assess the information content of the map, which is directly related to consumer card properties. The value and usefulness of the map are determined by its information content. The descriptiveness of the map is determined by the proportion of the information contained there, through which the user can solve the problem he is facing.

scholarly journals Functional Interactions between Sphingolipids and Sterols in Biological Membranes Regulating Cell Physiology

2009 ◽  
Vol 20 (7) ◽  
pp. 2083-2095 ◽  
Author(s):  
Xue Li Guan ◽  
Cleiton M. Souza ◽  
Harald Pichler ◽  
Gisèle Dewhurst ◽  
Olivier Schaad ◽  
...  
Keyword(s):  
Lipid Analysis ◽  
Sterol Composition ◽  
Convincing Evidence ◽  
Head Group ◽  
Cell Physiology ◽  
Membrane Composition ◽  
Plasma Membrane Fluidity ◽  
Simple Systems ◽  
In Cells

Sterols and sphingolipids are limited to eukaryotic cells, and their interaction has been proposed to favor formation of lipid microdomains. Although there is abundant biophysical evidence demonstrating their interaction in simple systems, convincing evidence is lacking to show that they function together in cells. Using lipid analysis by mass spectrometry and a genetic approach on mutants in sterol metabolism, we show that cells adjust their membrane composition in response to mutant sterol structures preferentially by changing their sphingolipid composition. Systematic combination of mutations in sterol biosynthesis with mutants in sphingolipid hydroxylation and head group turnover give a large number of synthetic and suppression phenotypes. Our unbiased approach provides compelling evidence that sterols and sphingolipids function together in cells. We were not able to correlate any cellular phenotype we measured with plasma membrane fluidity as measured using fluorescence anisotropy. This questions whether the increase in liquid order phases that can be induced by sterol–sphingolipid interactions plays an important role in cells. Our data revealing that cells have a mechanism to sense the quality of their membrane sterol composition has led us to suggest that proteins might recognize sterol–sphingolipid complexes and to hypothesize the coevolution of sterols and sphingolipids.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Lithography below 100 nm

1983 ◽  
Vol 41 ◽  
pp. 96-99
Author(s):  
L. D. Jackel
Keyword(s):  
Spatial Distribution ◽  
Electron Beam ◽  
Electron Scattering ◽  
Electron Beam Lithography ◽  
Substrate Material ◽  
Local Electron ◽  
Electron Dose ◽  
Positive Resist ◽  
Exposure Process

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.

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