scholarly journals Biochemical changes in cotton plants due to infestation by cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

2017 ◽  
Vol 9 (1) ◽  
pp. 382-388 ◽  
Author(s):  
V. S. Nagrare ◽  
J. Annie Sheeba ◽  
Paresh Bhoyar ◽  
Bhausaheb Naikwadi

The study on biochemical changes in cotton plants (Gossypium hirsutum L.) due to infestation by cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) was conducted at CICR Nagpur during 2014-15. Total protein contents estimated from the shoots of the healthy plants (4.29 mg/g) indicated 50.5% increase over the healthy plants (2.85 mg/g). Total phenol content increased by 185.7% in the mealybug infested plants (0.20μg/g) over the healthy plants (0.07μg/g). Insignificant difference in the level of total soluble sugar was observed in mealybug infested plants (1.00μg/g) as compared to healthy plants (0.90μg/g). Total reducing sugar was found to be unaffected with the mealybug infestation. Although there was depletion in all the photosynthetic pigments viz., chlorophyll a (19.1%), chlorophyll b (23.7%), total chlorophyll (21.2%) and carotenoids (20.8%) due to the mealybug infestation, these values were not statistically different in the healthy plants. This is the first report on biochemical changes in cotton plant due to infestation of P. solenopsis.

2016 ◽  
Vol 56 (2) ◽  
pp. 111-115 ◽  
Author(s):  
El-Zahi Saber El-Zahi ◽  
Safwat Abd El-Salam Aref ◽  
Samy Kamal Mohammad Korish

Abstract The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a polyphagous sap sucking insect with a wide geographical and host range causing severe losses in economically important crops. This study represents the first record of P. solenopsis as a new insect attacking cotton plants (Gossypium barbadense var. Giza 86) in Kafr El-Sheikh governorate, Egypt. The insect was noticed on cotton plants for the first time during its growing season of 2014. The mealybug specimens were collected from infested cotton plants and identified as P. solenopsis. In an attempt to control this pest, eight toxic materials viz., imidacloprid, thiamethoxam, flonicamid, emamectin-benzoate, chlorpyrifos, methomyl, deltamethrin and mineral oil (KZ-oil), belonging to different chemical groups, were tested for their influence against P. solenopsis on cotton under field conditions. Methomyl, imidacloprid, thiamethoxam and chlorpyrifos showed the highest efficacy against P. solenopsis recording 92.3 to 80.4% reduction of the insect population. Flonicamid, emamectin-benzoate and KZ-oil failed to exhibit sufficient P. solenopsis control.


2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Om Prakash Meena ◽  
Rishi Kesh Meena

Mitragyna parvifolia is a medicinally important plant which is native to India and Shrilanka. This plant is widely used by tribal people of Rajasthan and other Ayurvedic practitioners. Normal leaf and leaf gall of Mitragyna parvifolia were collected and categorized in normal (healthy leaf) and galled leaf. In the present investigation an attempt was made to study the changes of some metabolites during the gall formation on leaf of Mitragyna parvifolia attacked by gall insect. Total soluble sugar, total phenol and peroxidase activities were found high in gall leaves as compared to healthy leaves but chlorophyll contents and polyphenol oxidase activities were recorded high in normal leave and protein contents were slightly high in normal leaves as compared to infected or gall leaves.


Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Pedro García-Caparrós ◽  
Cristina Velasquez Espino ◽  
María Teresa Lao

The reuse of drainages for cultivating more salt tolerant crops can be a useful tool especially in arid regions, where there are severe problems for crops water management. Dracaena deremensis L. plants were cultured in pots with sphagnum peat-moss and were subjected to three fertigation treatments for 8 weeks: control treatment or standard nutrient solution (D0), raw leachates from Chrysalidocarpus lutescens H. Wendl plants (DL) and the same leachate blending with H2O2 (1.2 M) at 1% (v/v) (DL + H2O2). After harvesting, ornamental and biomass parameters, leaf and root proline and total soluble sugar concentration and nutrient balance were assessed in each fertigation treatment. Plant height, leaf and total dry weight had the highest values in plants fertigated with leachates with H2O2, whereas root length, leaf number, RGB values and pigment concentration declined significantly in plants fertigated with leachates from C. lutescens with or without H2O2. The fertigation with leachates, regardless of the presence or absence of H2O2 increased root and leaf proline concentration. Nevertheless, root and leaf total soluble sugar concentration did not show a clear trend under the treatments assessed. Regarding nutrient balance, the addition of H2O2 in the leachate resulted in an increase in plant nutrient uptake and efficiency compared to the control treatment. The fertigation with leachates with or without H2O2 increased nitrogen and potassium leached per plant compared to plants fertigated with the standard nutrient solution. The reuse of drainages is a viable option to produce ornamental plants reducing the problematic associated with the water consumption and the release of nutrients into the environment.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1313
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Md. Soyel Rana ◽  
...  

The effect of exogenously applied putrescine (Put) on salt stress tolerance was investigated in Panax ginseng. Thirty-day-old ginseng sprouts were grown in salinized nutrient solution (150 mM NaCl) for five days, while the control sprouts were grown in nutrients solution. Putrescine (0.3, 0.6, and 0.9 mM) was sprayed on the plants once at the onset of salinity treatment, whereas control plants were sprayed with water only. Ginseng seedlings tested under salinity exhibited reduced plant growth and biomass production, which was directly interlinked with reduced chlorophyll and chlorophyll fluorescence due to higher reactive oxygen species (hydrogen peroxide; H2O2) and lipid peroxidation (malondialdehyde; MDA) production. Application of Put enhanced accumulation of proline, total soluble carbohydrate, total soluble sugar and total soluble protein. At the same time, activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase in leaves, stems, and roots of ginseng seedlings were increased. Such modulation of physio-biochemical processes reduced the level of H2O2 and MDA, which indicates a successful adaptation of ginseng seedlings to salinity stress. Moreover, protopanaxadiol (PPD) ginsenosides enhanced by both salinity stress and exogenous Put treatment. On the other hand, protopanaxatriol (PPT) ginsenosides enhanced in roots and reduced in leaves and stems under salinity stress condition. In contrast, they enhanced by exogenous Put application in all parts of the plants for most cases, also evidenced by principal component analysis. Collectively, our findings provide an important prospect for the use of Put in modulating salinity tolerance and ginsenosides content in ginseng sprouts.


2019 ◽  
Vol 47 (4) ◽  
pp. 1252-1257
Author(s):  
Ying JIAN ◽  
Guolin WU ◽  
Donghui ZHOU ◽  
Zhiqun HU ◽  
Zhenxuan QUAN ◽  
...  

Wax apple (Syzygium samarangense) is an important tropical fruit tree cultivated in Southeast Asian. It produces red pear-like shape fruits. The fruit flesh is considered high in antioxidants, phenolics, and flavonoids that have a potential to contribute to the human healthy diet, and was proved to have anti-inflammatory and antimicrobial characteristics. To allow year-round marketing of high quality wax apple fruit, growers always perform shading to inhibit new flushes so as to repress vegetative growth and promote reproductive growth. To investigate the effect of shading on carbohydrates, wax apple trees were shaded with sun shade nets under field conditions. The effects of shading on shoot growth were studied and leaf carbohydrate levels of the trees were determined. The results showed that shading inhibit the the growth of the terminal shoots and promoted bud dormancy. Shading also reduced total soluble sugar, sucrose, glucose, fructose, and starch levels of leaves. The results suggested that shading reduced carbohydrate accumulation and repressed vegetative growth.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********


2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza

Quantification of total soluble sugars (as glucose) in plant tissue extracts via the sulfuric phenol method adapted for 96 well plates.


2003 ◽  
Vol 60 (2) ◽  
pp. 239-244 ◽  
Author(s):  
José Carlos da Silva ◽  
José Donizeti Alves ◽  
Amauri Alves de Alvarenga ◽  
Marcelo Murad Magalhães ◽  
Dárlan Einstein do Livramento ◽  
...  

One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and sucrose synthase. In plants with high level of carbon reserve, no increments in total soluble sugar levels or in enzymatic activity were observed. Regardless of treatments or plants physiological state, no differences in transpiration or stomatal conductance were observed, demonstrating the stomatal control of transpiration. Photosynthesis was stimulated with the use of 0.5 and 1.0 % sucrose only in depauperate plants. Coffee seedling spraying with sucrose is only efficient for depauperate plants, at the concentration of 1.0%.


2012 ◽  
Vol 32 (2) ◽  
pp. 334-343 ◽  
Author(s):  
Clarissa Damiani ◽  
Eduardo Ramirez Asquieri ◽  
Moacir Evandro Lage ◽  
Rodrigo Almeida de Oliveira ◽  
Flavio Alves da Silva ◽  
...  

The objective of this study was to add value to araça and marolo fruits by developing jams and verifying changes in their physical, chemical, and microbiological parameters during storage. The analyses were carried out every 2 months. From the results, it was shown that the levels of moisture (35.89 - 26.34%), lipids (0.43 - 0.27%), sucrose (30.62 - 28.98%), total pectin (0.83 - 0.50%), soluble pectin (0.52 - 0.38%), total phenolic compounds (180.31 - 135.52 mg.GAE 100 g-1), and organic acids (401.1 - 68.5 µg.g-1 citric acid) decreased during storage. However, the levels of protein (0.83 - 0.95%), carbohydrate (62.52 - 72.5%, calories (257,11 - 295,931 kcal), fiber (0.72 - 1.4%), total soluble sugar (62.52 - 70.44%), reducing sugar (32.05 - 41.41%), soluble solids (68.4 - 72.18 °Brix), consistency (0.33 - 0.44 N), total antioxidant potential (11.3 - 22.63%), and color (a* 7.56 - 9.49, and b* 8.63 - 10.49) increased during 1-year storage. The quality of the fruit jams studied was in accordance with the microbiological standards established by the Brazilian legislation. It was concluded that the mixed araça and marolo jam can be stored for 1 year without the addition of additives.


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