scholarly journals Effect of different spawn rates and substrate supplementation on yield of Indian Oyster mushroom, Pleurotus pulmonarius (Fr.) Quel.

2017 ◽  
Vol 9 (3) ◽  
pp. 1406-1410
Author(s):  
Joginder Pal ◽  
Ranjna Sharma ◽  
Manohar Lal ◽  
B. C. Suman

A study was conducted to evaluate the effect of different spawn rates and substrate supplementation on yield of Pleurotus pulmonarius (Fr.) Quel. Among six spawn rates viz., 0.5%, 1%, 2%, 4%, 6% and 8%, respectively tried on wheat straw substrate, the spawn run was fastest (10.50 days) when spawn dose was 8%, followed by 6%, 4%, 2%, 1% and 0.5%, respectively. The pinheads appeared in 12.27 days by using spawn @ 8%, which proved to be the best spawn dose followed by 6%, 4%, 2%, 1% and 0.5%, respectively. Highest yield of 168.7 per 200g dry substrate was achieved @ 8% spawn rate. Lesser yields were recorded when spawn rate was reduced. The results also reveals the significantly highest biological efficiency of 84.33% at 8% spawn rate followed by 6%, 4%, 2%, 1% and 0.5%, respectively. It was concluded that spawn run was rapid at higher spawn rate but there was not much difference in yield when spawn dose was increased from 4 to 8%. Considering spawn cost and performance shown by different doses, 2-4% was found optimum dose for its cultivation.In case of substrate supplementation, wheat straw supplemented with cotton seed meal supported maximum mycelial growth (10.50 days of inoculation) and took mini-mum time for pinheads initiation (13.67 days). Similarly, maximum yield (155.3g) with biological efficiency of 77.65% was recorded on wheat straw supplemented with cotton seed meal followed by supplementation of saw dust, wheat straw (control), calcium ammonium nitrate (CAN), ammonium nitrateand urea, respectively. These studies will help to mushroom growers for selecting the most suitable spawn rate and also opens viable option of supplementation as wheat straw + cotton seed meal for better growth behaviour and optimum yield potential of Pleurotus pulmonarius as well as other oyster mushrooms cultivation.

1982 ◽  
Vol 99 (2) ◽  
pp. 241-248 ◽  
Author(s):  
N. Sriskandarajah ◽  
R. C. Kellaway

SUMMARYEffects of treating wheat straw with caustic soda solution and of providing a cottonseed meal supplement were studied with 40 Friesian heifers (280 kg live weight) in individual pens. Responses were measured in terms of food intake, growth rate and food conversion ratio.Wheat straw was coarsely-milled and fedad libitum, untreated (U) or alkali-treated (T); both types of straw were supplemented with urea and minerals. Half the animals on each type of straw were fed 500 g cotton-seed meal (C/day.Straw intake was higher with T than with U (P< 0·01) and unaffected by the cotton-seed meal supplement, being 4·53, 4·71, 6·58 and 6·56 kg/day on treatments U, UC, T and TC respectively. Alkali treatment and the cotton-seed meal supplement improved live-weight change (P< 0·001), being –6, 189, 334 and 495 g/day respectively.In vivodigestible organic matter in the dry matter, calculated from lignin ratios, was 59, 60, 64 and 64 respectively.Rumen VFA concentrations were significantly higher, molar proportions of acetic acid lower, propionic andn-butyrie acids higher in animals on T than on U. Plasma urea and rumen ammonia concentrations were lower on treatment T than on U.It was concluded that wheat straw which is coarsely-milled, NaOH-treated and sprayed with urea and minerals provides sufficient nutrients to allow cattle to grow and that additional gain obtained by feeding cotton-seed meal was predictable in terms of its energy contribution to the diet.


1987 ◽  
Vol 108 (1) ◽  
pp. 211-219 ◽  
Author(s):  
K. Amaning-Kwarteng ◽  
R. C. Kellaway

SummaryGrowth and digestibility trials were conducted to investigate responses of young cross-bred sheep to alkali-treated straws and supplements. Oat and wheat straws were coarsely milled, sprayed with NaOH solution and then sprayed with a solution containing N, S, P, Cu and Co, and mixed with powdered limestone. The treated straws were fedad libitumwith no supplement (0) or with 150 g/day of either cotton-seed meal (CSM) or whole barley grain (B). In a preliminary trial to study the effect of frequency of ort collection on intake of treated straw, consumption decreased (P< 0·05) when frequency of ort collection was reduced from once daily to once weekly. This was associated with an increase in the neutral detergent fibre content of the orts. During the growth trial, which lasted 105 days, intake of treated straw increased by 34%.When no supplement was fed, digestibility of treated oat straw was higher (P < 0·05) and dry-matter intake lower (P< 0·05) than that of treated wheat straw. However, calculated intakes of metabolizable energy (ME) and measurements of growth and wool production were similar on the two straws (P> 0·05).When supplements were fed, there was no effect on intake of the treated straws. Mean live-weight gains were 51·4, 80·2 and 77·3 g/day on treatments 0, CSM and B respectively (P< 0·01). This was associated with higher intakes of ME (P< 0·01) which accounted for 73% of the variation in live-weight gains. Mean clean wool growth was 7·6, 10·5 and 9·3 g/day on treatments 0, CSM and B respectively (P< 0·01). This effect was associated more with nitrogen (N) absorption than with ME intake (80 and 48% of the variation in wool growth respectively).It was concluded that the treated straws provided sufficient nutrients for modest growth in young sheep, that the supplements had no effect on intake of the treated straws and that additional live-weight gain and wool growth obtained by feeding CSM and B were associated with energy intake and nitrogen absorbed respectively.


Author(s):  
Pinkal Patel ◽  
Ratna Trivedi

The Milky mushroom, Calocybe Indica was cultivated on different agricultural substrate, paddy straw, wheat straw, sugarcane trace and mango dry leaves. The spawning was done by sterilization of all the four substrate. The bags were kept in mushroom growing room with the maintenance of temperature and humidity 30̊ c-35̊ c and 70-80 % respectively. The minimum days requires for completion of spawn run (18.4 days), primordial formation (25.2 days) and days for first harvest (32.4 days) was first observed on cultivation with Paddy straw.  The maximum yield on fresh weight basis and biological efficiency (134.86 %) was also found to be as the same treatment with the Paddy straw as a substrate. The biological efficiency of wheat straw was at par with Sugarcane trace as substrate which was 85.07 % and 85.02 % respectively.


1998 ◽  
Vol 130 (4) ◽  
pp. 515-537 ◽  
Author(s):  
O.N. Morris ◽  
V. Converse ◽  
P. Kanagaratnam ◽  
J.-C. Coté

AbstractOne hundred and twoBacillus thuringiensisBerliner strains isolated from six different types of Canadian soil and dust from different grain storage bins were cultured in shake flasks containing Great Northern White Bean (GNWB) protein concentrate (48.6% protein) as the main nitrogen source and dextrose as the main carbohydrate source. The resulting endotoxins were bioassayed against the bertha armyworm,Mamestra configurataWlk. Thirty-three percent of soil and 66% of grain dust samples were positive forB. thuringiensis. The bacterium was found most frequently in organic-rich soil. The four most toxic soil isolates (which were seven to 15 times more toxic than the international standard, HD1-S-1980), and two nontoxic grain dust isolates were characterized by serological typing, biochemical analysis, carbohydrate utilization, plasmid profile analysis, protein profile analysis using sodium dodecyl sulfate – polyacrylamide gels, and polymerase chain reaction. Four isolates were determined to be subsp.kurstakicontaining 130–140 and 63–65 kDa proteins, and two isolates (tested for comparison) were subsp.canadensiscontaining 31 and 38 kDa proteins. Nonpyramidal-crystal-producing strains did not grow well in culture media containing GNWB, degossypellized cotton seed meal (61% protein), defatted soy flour (55% protein), or peptone as nitrogen sources. Excess of GNWB protein concentrate in shake flask culture media (30 g/L) inhibited bacterial growth and reduced the toxicity of isolate A1.2/72 subsp.kurstaki, which was the most toxic soil isolate. Isolate A1.2/72, which was 15 times more toxic for bertha armyworm larvae than the international standard (HD1-S-1980), contained threecry1Agenes (cry1Aa,cry1Ab, and cry1Ac), whereas HD-1 lacked thecry1Abgene. This strain was synergistic with strain HD-551 subsp.kenyae(cry1A,cry2A, andcry1Bgenes) but not with HD-133 subsp.aizawai(cry1Ab,cry1B,cry1C, andcry1Dgenes) when the strains were cultured together in a cotton seed meal medium and fed toM. configurata. The growth rate, economic yield, and toxicity of the new isolate, A1.2/72, produced in a 14-L laboratory fermenter declined when the fermentation ingredients were tripled. We believe that the indigenous strain A1.2/72 warrants further research development for bertha armyworm control.


1986 ◽  
Vol 55 (1) ◽  
pp. 179-186 ◽  
Author(s):  
Leibholz Jane

1. Pigs (n 195) between 21 and 49 d of age were given a basal diet containing wheat, wheat gluten and cotton-seed meal which was supplemented with one of nine protein concentrates or free L-lysine. The diets were given ad lib. in two experiments.2. Increasing the lysine content of the diets from 6.9 to 10.1 g/kg increased the weight gains of the pigs from 89 to 317 g/d in the first experiment. In the second experiment the weight gains were increased from 68 to 213 g/d by increasing the lysine content of the diets from 5.9 to 9.5 g/kg.3. The utilization of lysine from the protein concentrates was compared with the utilization of free L-lysine.4. The utilization of lysine for weight gain compared with free lysine was 0.86–0.88 for meat meals, 0.95–0.99 for soya-bean meal, 0, 69–0.75 for cotton-seed meal, 0.90 for lupins (Lupinus augustifoh) and 0.99 for milk.


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