Shadow enhancers' objective seems to be to establish robust growth patterns independent of genetic or environmental stress
Shadow enhancers' objective seems to be to establish robust growth patterns, independent of genetic or environmental stress. Multiple enhancers give more ability to encode intricate biological functions. Shadow enhancers are more conserved than other enhancers after being identified in the genome. Most shadow enhancer knockout research was undertaken in lab conditions that do not reflect natural scenarios. Many interactions across shadow enhancers (whether synergistic or repressive) are comparable to interactions inside a single enhancer between TF-binding sites. Because the length of DNA that can function as an enhancer is likely to be limited, multiple enhancers can be useful in regulating gene expression in complex ways.Despite its widespread presence in animal genomes, the evolutionary origins of shadow enhancies remain unknown.Most alterations in genome-wide association studies are projected to alter enhancer sequences. Patient-wide genome sequencing showed an increasing number of unusual non-coding mutations that change developmental genes and are linked to disease. It is possible that disease-causing non-Coding mutations preferentially impact genes without shadow enhancers or increase enhancer activity not dampened by the availability of enhancers. Alternatively, shadow enhancer changes may have some impact on target gene expression, which may be amplified by other mutations or the environment.