scholarly journals PROSPECTING OF PHENYLPROPANOIDS AND TERPENOIDS IN EAST INDIAN SANDALWOOD (SANTALUM ALBUM L.)

2017 ◽  
Author(s):  
Biswapriya Biswavas Misra
Keyword(s):  
Biosynthetic Pathway ◽  
Santalum Album ◽  
Sesquiterpene Synthase ◽  
Young Tree ◽  
East Indian ◽  
Sandalwood Oil ◽  
Santalum Album L ◽  
Dxp Synthase

The East Indian Sandalwood, Santalum album L., a tropical woody tree, deposits sesquiterpenoid rich essential oil in the heartwood. In view of its century-old traditional uses in perfumery and healthcare this investigation in prospecting of phenylpropanoid and terpenoids from in vitro tissues, in comparison to the in vivo trees, was undertaken to throw light on the phytochemistry of the healthcare molecules, explore in vitro tissues as alternate avenues for the supply and profiling of metabolites as a pre-requisite towards future sesquiterpenoid biosynthetic studies in this plant. Important phenylpropanoid enzymes i.e., phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD) and lignin peroxidases (POX) and their products, i.e., phenolic, hydroxycinnamic and benzoic acid, flavonoid, anthocyanin and lignin contents showed concomitant increase with lignification/ vascularization as visualized with histochemical analyses. HPLC analysis revealed identities and quantities of 20 phenolics, whereas LC-MS analysis identified ~52 phenylpropanoids through out development. Differential accumulation of terpenoid biosynthetic pathway enzymes, i.e., DXP synthase, HMGCoA reductase and sesquiterpene synthase and metabolites i.e., sesquiterpenoid hydrocarbons and alcohols, photosynthetic pigments and farnesylated intermediates were evident from GC and HPTLC analyses. A putative sesquiterpene synthase (~60 kDa) was partially purified from leaves. GC-MS/ MS studies revealed the presence of ~47 volatile constituents in sandalwood. The fragrant sandalwood oil constituent, β-santalol was isolated from somatic embryos and characterized by 13C-NMR, ESI-MS, LC-APCI-MS, MALDI-ToF, GC-MS/ MS and FTIR analyses whereas the bioactive constituent α-santalol, bulk-purified from sandalwood oil by HPTLC, RLCCC and column chromatography.Tissue extracts rich in terpenoids and phenylpropanoids revealed antimicrobial, antioxidant, α-amylase inhibition, anti-tyrosinase and neuroprotective potentials in vitro. Sandalwood oil and α-santalol showed strong in vivo antioxidant and anti-hyperglycemic potentials.Homology-based cloning using degenerate primers, yielded partial clones of a housekeeping gene (actin), a phenylpropanoid gene (CAD) and 4 terpenoid biosynthetic pathway genes (DXP synthase, FPP synthase, monoterpene synthase and sesquiterpene synthase), that showed homology and phylogenetic relationships with other plant genes. Homology-based modeling revealed their protein structure-function relationships, i.e., catalytic sites, domains and motifs. Structures were validated by Ramachandran plots. Transcriptional profiling by sqRT-PCR revealed higher expression levels of five transcripts in callus and young tree.

scholarly journals East Indian sandalwood (Santalum album L.) oil confers neuroprotection and geroprotection in Caenorhabditis elegans via activating SKN-1/Nrf2 signaling pathway

RSC Advances ◽  
2018 ◽  
Vol 8 (59) ◽  
pp. 33753-33774 ◽  
Author(s):  
A. Mohankumar ◽  
G. Shanmugam ◽  
D. Kalaiselvi ◽  
C. Levenson ◽  
S. Nivitha ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Signaling Pathway ◽  
Folk Medicine ◽  
Santalum Album ◽  
East Indian ◽  
Beneficial Effects ◽  
Nrf2 Signaling Pathway ◽  
Sandalwood Oil ◽  
Santalum Album L

East Indian Sandalwood Oil (EISO) has diverse beneficial effects and has been used for thousands of years in traditional folk-medicine for treatment of different human ailments.

scholarly journals Comparative Transcriptome Profiling of High and Low oil yielding Santalum album L.

2021 ◽  
Author(s):  
Tanzeem Fatima ◽  
Rangachari Krishnan ◽  
Ashutosh Srivastava ◽  
Vageeshbabu S. Hanur ◽  
M. Srinivasa Rao
Keyword(s):  
Transcriptome Profiling ◽  
Santalum Album ◽  
Rna Seq ◽  
Improvement Program ◽  
East Indian ◽  
Kegg Pathways ◽  
Oil Biosynthesis ◽  
Genome Wide ◽  
A Genome ◽  
Santalum Album L

East Indian Sandalwood (Santalum album L.) is highly valued for its heartwood and its oil. There have been no efforts to comparative study of high and low oil yielding genetically identical sandalwood trees grown in similar climatic condition. Thus we intend to study a genome wide transcriptome analysis to identify the corresponding genes involved in high oil biosynthesis in S. album. In this study, 15 years old S. album (SaSHc and SaSLc) genotypes were targeted for analysis to understand the contribution of genetic background on high oil biosynthesis in S. album. A total of 28,959187 and 25,598869 raw PE reads were generated by the Illumina sequencing. 2.12 million and 1.811 million coding sequences were obtained in respective accessions. Based on the GO terms, functional classification of the CDS 21262, & 18113 were assigned into 26 functional groups of three GO categories; (4,168; 3,641) for biological process (5,758;4,971) cellular component and (5,108;4,441) for molecular functions. Total 41,900 and 36,571 genes were functionally annotated and KEGG pathways of the DEGs resulted 213 metabolic pathways. In this, 14 pathways were involved in secondary metabolites biosynthesis pathway in S. album. Among 237 cytochrome families, nine groups of cytochromes were participated in high oil biosynthesis. 16,665 differentially expressed genes were commonly detected in both the accessions (SaHc and SaSLc). The results showed that 784 genes were upregulated and 339 genes were downregulated in SaHc whilst 635 upregulated 299 downregulated in SaSLc S. album. RNA-Seq results were further validated by quantitative RT-PCR. Maximum Blast hits were found to be against Vitis vinifera. From this study we have identified additional number of cytochrome family in SaHc. The accessibility of a RNA-Seq for high oil yielding sandalwood accessions will have broader associations for the conservation and selection of superior elite samples/populations for further genetic improvement program.

scholarly journals The Utilization of Protoporphyrin 9 in Heme Synthesis

Blood ◽  
1959 ◽  
Vol 14 (4) ◽  
pp. 476-485 ◽  
Author(s):  
MOISES GRINSTEIN ◽  
ROBIN M. BANNERMAN ◽  
CARL V. MOORE
Keyword(s):  
Cell Membrane ◽  
Human Blood ◽  
Biosynthetic Pathway ◽  
Aminolevulinic Acid ◽  
Thalassemia Major ◽  
Heme Synthesis ◽  
Equivalent Quantity ◽  
Chicken Erythrocytes

Abstract The experiments described in this communication demonstrate that C14-tagged protoporphyrin 9 can be incorporated into the heme during the biosynthesis of hemoglobin. 1. In vitro observations: (a) C14 protoporphyrin 9 was found to be incorporated into heme by hemolysates of chicken and human blood incubated at 37 C. The degree of incorporation by washed chicken erythrocytes was less, presumably because the protoporphyrin was not readily transferred across the cell membrane. Incorporation by hemolysates was inhibited completely at 1 x 10-2 M KCN at 4 C., markedly by 1 x 10-2 M KCN at 37 C. and partially by 1 x 10-3 M Pb at 37 C. (b) The degree of incorporation was reduced by the addition of an equivalent quantity of delta-aminolevulinic acid. Furthermore, the incorporation of glycine-2-C14 into heme was reduced by the addition of an equivalent quantity of protoporphyrin 9. 2. In vivo observations: Intravenously administered C14 protoporphyrin was incorporated into the circulating hemoglobin of two rabbits with a phenylhydrazine-induced hemolytic anemia. These observations provide support for the view that protoporphyrin 9 itself is a true direct precursor of hemoglobin, in the biosynthetic pathway between porphobilinogen and heme. Comparative studies of rates of incorporation of C14 protoporphyrin 9 and its precursors into heme in vitro may provide a useful tool for the study of heme synthesis in normal and pathologic conditions. For instance, it was shown that hemolysates from the blood of patients with thalassemia major, with poor iron and glycine utilization, rapidly incorporated the tagged protoporphyrin into heme.

scholarly journals The synthesis of murine ferrochelatase in vitro and in vivo

1988 ◽  
Vol 254 (3) ◽  
pp. 799-803 ◽  
Author(s):  
S R Karr ◽  
H A Dailey
Keyword(s):  
Membrane Protein ◽  
Biosynthetic Pathway ◽  
Integral Membrane Protein ◽  
Mitochondrial Proteins ◽  
Isolated Mitochondria ◽  
Mitochondrial Inner Membrane ◽  
Carbonyl Cyanide ◽  
A Cell

Ferrochelatase (protohaem ferro-lyase, EC 4.99.1.1), the terminal enzyme of the haem-biosynthetic pathway, is an integral membrane protein of the mitochondrial inner membrane. When murine erythroleukaemia cells are labelled in vivo with [35S]methionine, lysed, and the extract is immunoprecipitated with rabbit anti-(mouse ferrochelatase) antibody, a protein of Mr 40,000 is isolated. However, when isolated mouse RNA is translated in a cell-free reticulocyte extract, a protein of Mr 43,000 is isolated. Incubation of this Mr 43,000 protein with isolated mitochondria resulted in processing of the Mr 43,000 precursor to the Mr 40,000 mature-sized protein. Addition of carbonyl cyanide m-chlorophenylhydrazone and/or phenanthroline inhibits this processing. These data indicate that ferrochelatase, like most mitochondrial proteins, is synthesized in the cytoplasm as a larger precursor and is then translocated and processed to a mature-sized protein in an energy-required step.

Plastoquinone-9 biosynthesis in cyanobacteria differs from that in plants and involves a novel 4-hydroxybenzoate solanesyltransferase

2012 ◽  
Vol 442 (3) ◽  
pp. 621-629 ◽  
Author(s):  
Radin Sadre ◽  
Christian Pfaff ◽  
Stephan Buchkremer
Keyword(s):  
Biosynthetic Pathway ◽  
In Vitro Assays ◽  
Transport Chain ◽  
In Vivo Labelling ◽  
Membrane Bound ◽  
Transformation Processes ◽  
Synechocystis Sp

PQ-9 (plastoquinone-9) has a central role in energy transformation processes in cyanobacteria by mediating electron transfer in both the photosynthetic as well as the respiratory electron transport chain. The present study provides evidence that the PQ-9 biosynthetic pathway in cyanobacteria differs substantially from that in plants. We identified 4-hydroxybenzoate as being the aromatic precursor for PQ-9 in Synechocystis sp. PCC6803, and in the present paper we report on the role of the membrane-bound 4-hydroxybenzoate solanesyltransferase, Slr0926, in PQ-9 biosynthesis and on the properties of the enzyme. The catalytic activity of Slr0926 was demonstrated by in vivo labelling experiments in Synechocystis sp., complementation studies in an Escherichia coli mutant with a defect in ubiquinone biosynthesis, and in vitro assays using the recombinant as well as the native enzyme. Although Slr0926 was highly specific for the prenyl acceptor substrate 4-hydroxybenzoate, it displayed a broad specificity with regard to the prenyl donor substrate and used not only solanesyl diphosphate, but also a number of shorter-chain prenyl diphosphates. In combination with in silico data, our results indicate that Slr0926 evolved from bacterial 4-hydroxybenzoate prenyltransferases catalysing prenylation in the course of ubiquinone biosynthesis.

scholarly journals Cendana (Santalum album L.) sebagai tanaman penghasil minyak atsiri

Kultivasi ◽  
2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Mira Ariyanti ◽  
Yenni Asbur
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Genetic Factors ◽  
Economic Value ◽  
Volatile Oil ◽  
Oil Extraction ◽  
Santalum Album ◽  
Functional Value ◽  
Sandalwood Oil ◽  
Santalum Album L

Abstract. Sandalwood is source of essential oil and as non-wood commodity of forest, which is potential and considered luxurious due to its distinctiveness hard wood and containing specific oil scent. Sandalwood oil can be produced from its wood by extracting from its tree trunks, twigs, brances, or root. The economic value of sandalwood plan is derived from its oil content (santalol) which has unique scent. A volatile oil contained in sandalwood is the sesquiterpenoid compound; among them are α-santalol dan b-santalol. Interaction between genetic factors of plant with its environment is a major factor that determines the growth and development of sandalwood plant that eventually affecting the volatile oil that can be produced. Engineering efforts are required against factors that  related with oil extraction in order to obtain its maximum production in both in quantity and quality. Sandalwood oil has high functional value, some of them are as the material for aromatic therapy which is particularly beneficial for human health, as cosmetic material, and as material for medicines.Keywords : sandalwood, essential oil, santalol Sari. Cendana merupakan sumber penghasil minyak atsiri dan merupakan komoditi hasil hutan bukan kayu yang potensial dan tergolong mewah karena sifat kayu terasnya yang khas dan mengandung minyak dengan aroma yang spesifik. Pembuatan minyak cendana dapat dilakukan dengan memanfaatkan batang kayu, ranting, cabang ranting, dan akar pohon cendana. Nilai ekonomi tanaman cendana didapat dari kandungan minyak (santalol) dalam kayu yang beraroma wangi yang khas. Minyak atsiri yang terkandung pada kayu cendana merupakan golongan senyawa sesquiterpenoid diantaranya α-santalol dan b-santalol. Interaksi antara faktor genetik tanaman dengan ling-kungan merupakan faktor utama yang menentukan pertumbuhan dan perkembangan tanaman cendana yang pada akhirnya berpengaruh terhadap produksi minyak atsiri yang dihasilkan. Diperlukan upaya rekayasa terhadap faktor-faktor yang terkait dengan ektraksi minyak cendana sehingga produksi maksimal dicapai baik secara kuantitas maupun kualitas. Minyak cendana memiliki nilai fungsi yang tinggi diantaranya sebagai bahan aroma terapi yang sangat bermanfaat bagi kesehatan manusia, bahan kosmetik, dan bahan untuk obat-obatan.Kata kunci : tanaman cendana, minyak atsiri, santalol

scholarly journals A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Brent Townshend ◽  
Joy S. Xiang ◽  
Gabriel Manzanarez ◽  
Eric J. Hayden ◽  
Christina D. Smolke
Keyword(s):  
Small Molecules ◽  
Biosynthetic Pathway ◽  
De Novo ◽  
Living Cells ◽  
Regulate Gene Expression ◽  
Liquid Handling ◽  
Regulate Gene

AbstractBiosensors are key components in engineered biological systems, providing a means of measuring and acting upon the large biochemical space in living cells. However, generating small molecule sensing elements and integrating them into in vivo biosensors have been challenging. Here, using aptamer-coupled ribozyme libraries and a ribozyme regeneration method, de novo rapid in vitro evolution of RNA biosensors (DRIVER) enables multiplexed discovery of biosensors. With DRIVER and high-throughput characterization (CleaveSeq) fully automated on liquid-handling systems, we identify and validate biosensors against six small molecules, including five for which no aptamers were previously found. DRIVER-evolved biosensors are applied directly to regulate gene expression in yeast, displaying activation ratios up to 33-fold. DRIVER biosensors are also applied in detecting metabolite production from a multi-enzyme biosynthetic pathway. This work demonstrates DRIVER as a scalable pipeline for engineering de novo biosensors with wide-ranging applications in biomanufacturing, diagnostics, therapeutics, and synthetic biology.

scholarly journals In vitro reconstitution of indolmycin biosynthesis reveals the molecular basis of oxazolinone assembly

2015 ◽  
Vol 112 (9) ◽  
pp. 2717-2722 ◽  
Author(s):  
Yi-Ling Du ◽  
Lona M. Alkhalaf ◽  
Katherine S. Ryan
Keyword(s):  
Molecular Basis ◽  
Biosynthetic Pathway ◽  
Therapeutic Agents ◽  
Trna Synthetase ◽  
Ring Assembly ◽  
Coa Ligase ◽  
Biochemical Assays ◽  
In Vitro Reconstitution

The bacterial tryptophanyl–tRNA synthetase inhibitor indolmycin features a unique oxazolinone heterocycle whose biogenetic origins have remained obscure for over 50 years. Here we identify and characterize the indolmycin biosynthetic pathway, using systematic in vivo gene inactivation, in vitro biochemical assays, and total enzymatic synthesis. Our work reveals that a phenylacetate–CoA ligase-like enzyme Ind3 catalyzes an unusual ATP-dependent condensation of indolmycenic acid and dehydroarginine, driving oxazolinone ring assembly. We find that Ind6, which also has chaperone-like properties, acts as a gatekeeper to direct the outcome of this reaction. With Ind6 present, the normal pathway ensues. Without Ind6, the pathway derails to an unusual shunt product. Our work reveals the complete pathway for indolmycin formation and sets the stage for using genetic and chemoenzymatic methods to generate indolmycin derivatives as potential therapeutic agents.

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