scholarly journals Sero-Detection of Leptospira hardjo in Cattle of Bhaktapur District of Nepal

2019 ◽  
Vol 7 (3) ◽  
pp. 378-381 ◽  
Author(s):  
Gaurav Rawal ◽  
Denusha Shrestha
Keyword(s):  
Antibody Test ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Sample Collection ◽  
Serum Samples ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Multiple Comparison Test ◽  
Test Kit ◽  
Elisa Data

Leptospira hardjo is the most commonly reported cause of leptospirosis among cattle globally. The objective of this study was to determine sero-detection of Leptospira hardjo in cattle of Bhaktapur district of Nepal. A cross-sectional study was conducted in cattle pockets located at 4 different village development committees (VDCs) in the Bhaktapur district of Nepal. The sample collection was done in cattle to determine the sero-detection of Leptospira hardjo from February 2014 to June 2014. A total of 176 serum samples were collected from four VDCs of Bhaktapur district namely Sipadol, Dhadikot, Duwakot and Nangkhel, selected purposively. Forty samples from Sipadol, 46 Dhadikot, 42 Duwakot and 48 from Nangkhel were collected. 5 ml of blood was collected aseptically from jugular vein using 5 ml sterile disposable syringe. After that blood was transferred to the plain vacutainer. The harvested sera were transferred to serum vials and stored at in -20˚c deep freeze of Central Veterinary Laboratory until used for ELISA test. For screening of Leptospira hardjo, the Leptospira hardjo antibody test kit, ELISA (Prionics, Netherlands) was used. ANOVA along with multiple comparison test Tukey was used to compare frequency of detection across different locations in Bhaktapur district using SAS 9.4. MS-Excel was used to manage ELISA data from four different VDCs and to extract information regarding frequency of detection. There was 5.11% sero-detection in cattle of Bhaktapur district. Location wise sero-detection was 5% in Sipadol, 4.3% in Dhadikot, 4.76% in Duwakot and 6.25% in Nangkhel. The study showed that the detection of Leptospira hardjo in cattle. There was no statistical difference (P>0.05) between location suggesting that cattle in all areas are equally at risk of this pathogen. Further study is suggested on isolation and identification of disease in Nepal. Int. J. Appl. Sci. Biotechnol. Vol 7(3): 378-381

scholarly journals SEROPREVALENCE OF SALMONELLA SPP. ON IMPORT BOARS FROM DENMARK TO SERBIA

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Nenad Stojanac ◽  
Ognjen Stevančević ◽  
Božidar Savić ◽  
Ivan Stančić ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Time Span ◽  
Sectional Study ◽  
Serum Samples ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
One Year ◽  
Swine Herds

The sera from 120 boars from 3 farrow-to-finish swine herds in Serbia were examined for Salmonella spp. antibodies in a cross-sectional study using an ELISA test. A total of 120 blood serum samples from boars imported from Denmark were examined. All boars were seronegative to Salmonella during the import, while after one year Salmonella spp. seroprevalence ranging from 0% to 45% was found in 3 herds using two tests in the time span of one year. Only on one farm during the import in 2011, after a year boars were still seronegative. A year later on the same farm, in boars imported in 2012 the seroprevalence was 30%. Seroprevalence of Salmonella spp. in boars tested on all farms was 24,17%.

scholarly journals Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for AssayingLeishmania infantumAntibodies in Dogs

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Daniela Proverbio ◽  
Eva Spada ◽  
Luciana Baggiani ◽  
Giada Bagnagatti De Giorgi ◽  
Roberta Perego
Keyword(s):  
Roc Curve ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Elisa Test ◽  
Standard Test ◽  
Confirmatory Test ◽  
Canine Leishmaniosis ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Test Kit

This study compares a rapid Immunospecific Kalazar Canine Rapid Spot IF with the gold standard test (indirect fluorescent antibody test (IFAT)) for detection ofLeishmania infantumspecific IgG serum antibodies in naturally exposed dogs. Serum samples were obtained from 89 healthy dogs and dogs affected by canine leishmaniosis (CanL). IgG-IFAT titers ≥80 were considered positive. Anti-L. infantumIgG antibodies were found in 54 samples with titers ranging from 1 : 80 to 1 : 5120. The performance of the rapid Immunospecific Kalazar was evaluated using a ROC curve. The area under the ROC curve of 0.957 was significantly different from 0.5 (), and therefore it can be concluded that the rapid Immunospecific Kalazar has the ability to distinguish canine sera with and withoutL. infantumIgG. The best performance of the test was at a cutoff >0 (sensitivity 92.6%, specificity 97%). The test can be used for disease screening if the cutoff is >0 (highest sensitivity, 92.6%) and is recommended as confirmatory test for the presence ofL. infantumIgG antibodies if the cutoff is set >2 (highest specificity, 100%).

scholarly journals Correlation between Proinflammatory cytokines and severity of COVID-19 within Palestinian Population

2021 ◽  
Author(s):  
Walid Basha ◽  
Zaher Nazzal ◽  
Yousef El-Hamshary ◽  
Anwar Odeh ◽  
Lama Hijjawi ◽  
...  
Keyword(s):  
Serum Level ◽  
Proinflammatory Cytokines ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Hospitalized Patients ◽  
Serum Samples ◽  
Cross Sectional ◽  
Tnf Α ◽  
Ifn Γ ◽  
Palestinian Population

AbstractCOVID-19 was characterized by cytokine storm and endothelial dysfunction in severely ill patients. As the severity of the infection was corelated with ethnicity, this study aimed to assess the correlation of proinflammatory cytokine serum level and COVID-19 symptoms within the Palestinian population. In cross-sectional study, serum samples of 27 non-hospitalized patients and 63 hospitalized patients SARS-CoV-2 infected patients, were tested for total antibodies, IL-6, TNF-α, IFN-γ and IL-1β using the ELISA test. Results showed that most common symptoms within patients were Joint pain, cough, and fever (73.3%, 69.7% and 50% respectively). Serum total antibodies (IGs) levels in non-hospitalized patients were higher than hospitalized patients ((44.7 COI and 9.2 COI). TNF-α and IL-6 were lower in non-hospitalized patients compared to hospitalized patients (48±17.9 pg/ml, 193.3±350.5 pg/ml respectively). On the other hand, IFN-γ, in non-hospitalized patients (1±2 IU/ml) was significantly higher than hospitalized patients (0.4±0.26 IU/ml). IL-1β was slightly lower in hospitalized patients (8.8±13.6 pg/ml) compared to non-hospitalized patients (12.5±24.5 pg/ml). Common mild symptoms of COVID-10 were negatively associated with proinflammatory cytokines serum level. In conclusion as it with other populations worldwide, IL-6 and TNF-α are playing a major role in the complications of SARS-CoV-2 infection. Monitoring the two cytokines is crucial for management and treatment of complicated consequence of COVID-19.

scholarly journals Seroprevalence of Human Fascioliasis Using ELISA in Sistan and Baluchestan Province, Southeast of Iran

Thrita ◽  
2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Hosnie Hoseini ◽  
Soudabeh Etemadi
Keyword(s):  
Fasciola Hepatica ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Chi Square ◽  
Cross Sectional ◽  
Human Fascioliasis ◽  
Chi Square Test ◽  
Statistical Estimations

Background: Fascioliasis is a zoonotic disease caused by the liver parasites, Fasciola hepatica, and F. gigantica. Objectives: The aim of this study was to determine the seroprevalence of fascioliasis in Zahedan and the surrounding towns (i.e., Zabol, Chabahar, Iranshahr, etc.) in the Southeast of Iran. Methods: This cross-sectional study was conducted in 2017. Based on statistical estimations, 251 human serum samples were collected randomly in Zahedan and surrounding towns (Zabol, Chabahar, Iranshahr, and others), then the samples were analyzed using indirect enzyme-linked immunosorbent assay (ELISA) test. A questionnaire was obtained from each individual. Statistical analysis was performed using the chi-square test in SPSS, version 20. Results: Of the 251 samples, the anti-fasciola antibody was detected in 6 (2.40%) samples. There was a significant relationship between the use of local freshwater vegetables and seropositivity to fasciolosis. Conclusions: This study showed that the prevalence of human fascioliasis was higher among women (2.70%) compared to men (0.0%), and raising health levels and health education can reduce the prevalence of human fascioliasis disease.

scholarly journals Mongooses (Urva auropunctata) as reservoir hosts of Leptospira species in the United States Virgin Islands, 2019–2020

2021 ◽  
Vol 15 (11) ◽  
pp. e0009859
Author(s):  
Hannah M. Cranford ◽  
A. Springer Browne ◽  
Karen LeCount ◽  
Tammy Anderson ◽  
Camila Hamond ◽  
...  
Keyword(s):  
Predictive Value ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The United States ◽  
Virgin Islands ◽  
Cross Sectional Study ◽  
Rt Pcr ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Predictive Values

During 2019–2020, the Virgin Islands Department of Health investigated potential animal reservoirs of Leptospira spp., the bacteria that cause leptospirosis. In this cross-sectional study, we investigated Leptospira spp. exposure and carriage in the small Indian mongoose (Urva auropunctata, syn: Herpestes auropunctatus), an invasive animal species. This study was conducted across the three main islands of the U.S. Virgin Islands (USVI), which are St. Croix, St. Thomas, and St. John. We used the microscopic agglutination test (MAT), fluorescent antibody test (FAT), real-time polymerase chain reaction (lipl32 rt-PCR), and bacterial culture to evaluate serum and kidney specimens and compared the sensitivity, specificity, positive predictive value, and negative predictive value of these laboratory methods. Mongooses (n = 274) were live-trapped at 31 field sites in ten regions across USVI and humanely euthanized for Leptospira spp. testing. Bacterial isolates were sequenced and evaluated for species and phylogenetic analysis using the ppk gene. Anti-Leptospira spp. antibodies were detected in 34% (87/256) of mongooses. Reactions were observed with the following serogroups: Sejroe, Icterohaemorrhagiae, Pyrogenes, Mini, Cynopteri, Australis, Hebdomadis, Autumnalis, Mankarso, Pomona, and Ballum. Of the kidney specimens examined, 5.8% (16/270) were FAT-positive, 10% (27/274) were culture-positive, and 12.4% (34/274) were positive by rt-PCR. Of the Leptospira spp. isolated from mongooses, 25 were L. borgpetersenii, one was L. interrogans, and one was L. kirschneri. Positive predictive values of FAT and rt-PCR testing for predicting successful isolation of Leptospira by culture were 88% and 65%, respectively. The isolation and identification of Leptospira spp. in mongooses highlights the potential role of mongooses as a wildlife reservoir of leptospirosis; mongooses could be a source of Leptospira spp. infections for other wildlife, domestic animals, and humans.

scholarly journals Hepatitis E Virus Infection Among Iranian Patients with Beta-Thalassemia

2020 ◽  
Vol 7 (3) ◽  
Author(s):  
Farahnaz Bineshian ◽  
Najmeh Dalvand ◽  
Seyed Masoud Hosseini ◽  
Zohreh Sharifi
Keyword(s):  
Hepatitis E Virus ◽  
Rna Extraction ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Beta Thalassemia ◽  
Serum Samples ◽  
Cross Sectional ◽  
Emerging Virus ◽  
Precautionary Measures

Background: The main transmission route of hepatitis E virus (HEV) is fecal-oral by contaminated water and food. Besides, it can be transmitted through infected blood of persons with subclinical infection. Objectives: The current study aimed to estimate the prevalence of HEV infection as an emerging virus among thalassemic patients. Methods: In this cross-sectional study, 110 thalassemia patients who were living in Tehran Province were selected. All serum samples were assessed for the presence of total anti-HEV antibodies (IgG and IgM). After RNA extraction from sera, cDNA was synthesized, and RT-PCR was used to determine the HEV-RNA. Results: The results of the ELISA test showed that 4.5% of thalassemia patients were positive for anti-HEV Ab. Also, the prevalence of HEV-RNA was 0.0% among thalassemia patients. Conclusions: Our findings may confirm that the risk of HEV infection via blood transmission among thalassemia patients is low in Iran. However, to prevent the possibility of HEV transmission, precautionary measures should be considered in blood recipients.

scholarly journals Second round statewide survey for estimation of the burden of active infection and anti-SARS-CoV-2 IgG antibodies in the general population of Karnataka, India

2021 ◽  
Author(s):  
M Rajagopal Padma ◽  
Prameela Dinesh ◽  
Rajesh Sundaresan ◽  
Siva Athreya ◽  
Shilpa Shiju ◽  
...  
Keyword(s):  
General Population ◽  
Population Survey ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Natural Immunity ◽  
Active Infection ◽  
Cross Sectional ◽  
Healthcare Facilities ◽  
Clinical Sensitivity ◽  
Test Kit

Objective: The second round of the serial cross sectional sentinel based population survey to assess active infection, seroprevalence, and their evolution in the general population across Karnataka was conducted. Additionally, a longitudinal study among participants identified as COVID 19 positive in the first survey round was conducted to assess the clinical sensitivity of the testing kit used. Methods: The cross sectional study of 41,228 participants across 290 healthcare facilities in all 30 districts of Karnataka was done among three groups of participants (low, moderate, and high risk). Consenting participants were subjected to real time reverse transcription polymerase chain reaction (RTPCR) testing, and antibody (IgG) testing. Results: Overall weighted adjusted seroprevalence of IgG was 15.6% (95% CI: 14.9 to 16.3), crude IgG prevalence was 15.0% and crude active prevalence was 0.5%. Statewide infection fatality rate (IFR) was estimated as 0.11%, and COVID 19 burden estimated between 26.1 to 37.7% (at 90% confidence). Clinical sensitivity of the IgG ELISA test kit was estimated as equal or more than 38.9%. Conclusion: The sentinel based population survey helped identify districts that needed better testing, reporting, and clinical management. The state was far from attaining natural immunity during the survey and hence must step up vaccination coverage and enforce public health measures to prevent the spread of COVD 19.

scholarly journals Role of human epididymis protein 4 for detection of ovarian carcinoma in adnexal masses: A pilot study

2016 ◽  
Author(s):  
Nidhi Bansal ◽  
A. Suneja ◽  
K. Guleria ◽  
N. B. Vaid ◽  
K. Mishra ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Cross Sectional Study ◽  
Ca 125 ◽  
Serum Samples ◽  
Cross Sectional ◽  
Adnexal Masses ◽  
Good Discriminator ◽  
Risk Of Malignancy ◽  
Risk Of Malignancy Index ◽  
Benign Tumours

Introduction: HE4 is a novel tumour biomarker used for early diagnosis of ovarian cancer. This study evaluated the diagnostic accuracy of HE4 alone and in combination with CA125, risk of malignancy index (RMI), risk of malignancy algorithm (ROMA). Methods: It was a cross sectional study conducted recruiting 88 women with adnexal masses who were planned for surgery. After baseline work up and ultrasound examination, serum samples were collected for estimation of CA 125 and HE4 levels. Serum HE4 levels were estimated using ELISA kit. RMI and ROMA score were calculated and diagnostic accuracy of HE4, CA 125, RMI, ROMA and their combination were compared. Cut off for HE4 and ROMA score were calculated using ROC curve. Results: Of 88 subjects, 66 were analyzed with 19 malignant (including 5 LMP) and 47 benign cases. The median value of HE4 among malignant cases was found to be significantly higher than among the benign cases. PPV and NPV of HE4 at a cut off 130.8 pMol/ml was 85.7% and 77.9% respectively. Highest PPV (88.9%) with acceptable NPV (80.7%) was found with ROMA followed by HE4 (PPV 85.7%; NPV 77.97%), RMI (PPV 76.92%; NPV 83%) and CA125 (PPV 52%; NPV 80.85%). Conclusion: HE4 levels were lower in Indian population both in malignant and benign tumours as compared to other studies. HE4 is a good discriminator and gives best accuracy when it is combined with CA125 in a logistic algorithm, ROMA.

scholarly journals The characteristics and risk factors of human papillomavirus infection: an outpatient population-based study in Changsha, Hunan

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bingsi Gao ◽  
Yu-Ligh Liou ◽  
Yang Yu ◽  
Lingxiao Zou ◽  
Waixing Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Protective Factor ◽  
Hpv Infection ◽  
Population Based ◽  
Cross Sectional Study ◽  
Categorical Variables ◽  
Chi Square ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Test Kit

AbstractThis cross-sectional study investigated the characteristics of cervical HPV infection in Changsha area and explored the influence of Candida vaginitis on this infection. From 11 August 2017 to 11 September 2018, 12,628 outpatient participants ranged from 19 to 84 years old were enrolled and analyzed. HPV DNA was amplified and tested by HPV GenoArray Test Kit. The vaginal ecology was detected by microscopic and biochemistry examinations. The diagnosis of Candida vaginitis was based on microscopic examination (spores, and/or hypha) and biochemical testing (galactosidase) for vaginal discharge by experts. Statistical analyses were performed using SAS 9.4. Continuous and categorical variables were analyzed by t-tests and by Chi-square tests, respectively. HPV infection risk factors were analyzed using multivariate logistic regression. Of the total number of participants, 1753 were infected with HPV (13.88%). Females aged ≥ 40 to < 50 years constituted the largest population of HPV-infected females (31.26%). The top 5 HPV subtypes affecting this population of 1753 infected females were the following: HPV-52 (28.01%), HPV-58 (14.83%), CP8304 (11.47%), HPV-53 (10.84%), and HPV-39 (9.64%). Age (OR 1.01; 95% CI 1–1.01; P < 0.05) and alcohol consumption (OR 1.30; 95% CI 1.09–1.56; P < 0.01) were found to be risk factors for HPV infection. However, the presence of Candida in the vaginal flora was found to be a protective factor against HPV infection (OR 0.62; 95% CI 0.48–0.8; P < 0.001). Comparing with our previous study of 2016, we conclude that the subtype distribution of HPV infection is relatively constant in Changsha. Our data suggest a negative correlation between vaginal Candida and HPV, however, more radical HPV management is required in this area for perimenopausal women and those who regularly consume alcohol.

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