scholarly journals Hemato-Biochemical Analyses of Lactating Cross-Bred Jersey Cattle at Kaski District, Nepal

2018 ◽  
Vol 35 (1) ◽  
pp. 243-247
Author(s):  
B. Regmi ◽  
K. R. Pande
Keyword(s):  
Hematological Parameters ◽  
Reference Value ◽  
Normal Range ◽  
Serum Samples ◽  
Jersey Cattle ◽  
Mineral Profile ◽  
Serum Biochemical ◽  
Biochemical Profiles ◽  
Biochemical Analyses ◽  
Set Up

Hemato-biochemical profiles have been used widely to identify the health problems associated with productive and reproductive disorders. The objective of this study was to set up a baseline hematological and serum biochemical values. The study was carried out in 50 lactating cross bred Jersey cattle of Kaski district ranging in age from 3-7 years. Non-pregnant, lactating, apparently healthy and stall-fed cattle were selected for sampling and these animals were regularly dewormed and vaccinated against common diseases. All the hematological parameters were determined on the same day of collection using auto analyzer. The samples were also tested for blood protozoans (Anaplasma, Babesia, Theleria and Trypanosoma), using thin and thick smears. The serum samples were collected in a sterile vial for serum biochemical analysis and preserved at -200C until used for the assays. All the hematological parameters were within the normal range. However, 2% of the total animals tested appeared Theleria positive. Eosinophil and basophils were within the normal range suggesting that the deworming was effective and there was no allergic reaction. Mineral profile particularly glucose (11.75±0.87) was found significantly (P<0.05) low. This suggests that cross Jersey cattle were prone to hypoglycemia related metabolic disorders and corrective measures should be employed for better production. The values obtained in this study within the range can be used cautiously as reference value at lactating stage of cross-bred Jersey cattle. Further detail study is needed to exacting the standard reference value for this breed of cattle.

Evaluation of a Commercial Enzymatic Method for the Determination of Total Serum Bile Acids

1980 ◽  
Vol 17 (6) ◽  
pp. 301-306 ◽  
Author(s):  
Per Tobiasson ◽  
Magnus Källberg
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Reference Range ◽  
Reference Value ◽  
Total Serum ◽  
Enzymatic Method ◽  
Normal Range ◽  
Serum Samples ◽  
Serum Bile Acids

An enzymatic method (Sterognost-3α® Flu, Nyegaard & Co, Oslo, Norway) for the determination of total serum bile acids has been evaluated, and reference values for fasting and postprandial total serum bile acids have been established. Precision was rather low in the lower normal range but satisfactory in the upper reference range and for elevated values. The upper reference value for fasting total serum bile acids was 5·0 μmol/l. Maximal postprandial elevations occurred at varying intervals after a test meal and the upper postprandial reference value was 12 μmol/l. Certain serum samples were also analysed by radioimmunological methods for conjugates of cholic and chenodeoxycholic acids. The values obtained with the enzymatic method correlated satisfactorily with those of the radioimmunoassay methods for most of the serum samples analysed. However, a number of samples with slightly elevated bile acid concentrations, as measured by the comparison radioimmunoassay methods, were found to have normal total serum bile acids when measured by the enzymatic method.

scholarly journals ANÁLISIS DE MINERALES SÉRICOS DE CERDAS EN LACTACIÓN EN DOS GRANJAS COMERCIALES

2021 ◽  
Vol 19 (suplemento) ◽  
Author(s):  
J Bellezze
Keyword(s):  
Atomic Absorption Spectrophotometry ◽  
Santa Fe ◽  
Normal Range ◽  
Serum Samples ◽  
Mean Values ◽  
Absorption Spectrophotometry ◽  
Significant Difference ◽  
Mineral Profile ◽  
Entre Rios ◽  
Mineral Concentrations

Mineral nutrition is essential for profitable production. Health affected is not provided proper levels minerals. Objective of the present work was to analyze mineral profile in serum of sows in lactation, province Santa Fe (A), Entre Rios (B) and fed with diets minerals of inorganic origin. Blood serum samples 68 sows, Farm A (in field) and Farm B (in stabled form) were analyzed. Mineral concentrations were determined by means of atomic absorption spectrophotometry; ANOVA was applied to these data. Mean values and their standard deviations obtained are: Calcium (Ca) (mg/dL): 8,61±3,44/ 8,11± 1,73; Phosphorus (P) (mg/dL): 2,66a±0,90/ 3,53b± 1,16; Magnesium (Mg) (mg/dL): 2,25a±0,30/ 1,89b±0,35; Sodium (Na) (mEq/L): 160,98a±15,47/ 138,53b±9,48; Potassium (K) (mEq/L): 3,42±0,24/ 3,58±0,33; Iron (Fe) (µg/dL): 62,74a±6,07/ 94,82b±19,19; Copper (Cu) (ppm): 1,63a±0,35/ 1,01b±0,32; Zinc (Zn) (ppm): 1,82a±0,5/ 1,50b±0,42, for farms A and B respectively. Na and Zn averages were observed slightly above upper limit of normal range. Copper in farm B was observed below normal range. Significant difference (“a” and “b” P <0.05) in P, Mg, Na, Fe, Cu and Zn was observed between two farms, this is due to difference in gestation system. Cu concentration tended to be less. Is that subject should continue to be studied.

scholarly journals Biochemical parameters of the giant anteater (Myrmecophaga tridactyla Linnaeus, 1758) of the Brazilian Cerrado

2018 ◽  
Vol 38 (1) ◽  
pp. 189-194
Author(s):  
Evelyn de Oliveira ◽  
Laura G. Vila ◽  
Thays de C. Trentin ◽  
Tiago de O. Jubé ◽  
Danieli B. Martins
Keyword(s):  
Forest Fires ◽  
Considerable Increase ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Laboratory Evaluation ◽  
Brazilian Cerrado ◽  
In Captivity ◽  
Serum Biochemical ◽  
Biochemical Profiles ◽  
Biochemical Analyses

ABSTRACT: The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species on Brazil’s list of species at risk of extinction mainly due to deforestation and forest fires. This has contributed to a considerable increase in detailed clinical case records of the treatment of wild species at veterinary institutions. However, the paucity of serum biochemical profiles of healthy giant anteaters has made it difficult to evaluate these animals, preventing diagnosis, treatment and prognosis. The objective of this work was to collect data about the biochemical profile of healthy giant anteaters from the Brazilian Cerrado raised in captivity, in order to better understand the physiological characteristics inherent to this species. Eighteen analytes from 12 healthy giant anteaters were measured. The following means and standard deviations were found in the biochemical analyses: albumin 3.29±0.33g/dL, ALT 15.49±7.98 IU/L, amylase 1037.92±149.04 IU/L, AST 21, 12±7.50 IU/L, total cholesterol 62.79±20.08mg/dL, HDL cholesterol 14.73±4.98mg/dL, LDL cholesterol 26.60±11.05mg/dL, VLDL cholesterol 2.14±1.06mg/dL, CK 111.61±70.16 IU/L, creatinine 1.05±0.37mg/dL, iron 194.64±81.17μg/dL, GGT 65.18±54.57 IU/L, glucose 103.71±29.63mg/dL, globulins 2.76±0.36g/dL, lipase 28.80±5.11 IU/L,TSP 6.05±0.56g/dL, triglycerides 10.71±5.29mg/dL, and urea 53.46±18.28mg/dL. The values found in this study can be used as references for the laboratory evaluation of giant anteaters living in conditions similar to those of this study. This is one of the first reports of biochemical examinations on giant anteaters of the Cerrado biome.

scholarly journals COMPARISON OF HEMATO-BIOCHEMICAL PARAMETERS BETWEEN APPARENTLY HEALTHY AND BOVINE TUBERCULOSIS AFFECTED CATTLE IN CHITTAGONG, BANGLADESH

2018 ◽  
Vol 16 (1) ◽  
pp. 53-57
Author(s):  
M. B. Hossain ◽  
M. M. Khan ◽  
M. A. Rumi ◽  
M. Ahammed ◽  
M. S. Bari
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Cross Sectional Study ◽  
Control Group ◽  
Serum Samples ◽  
Cross Sectional ◽  
Healthy Cattle ◽  
Serum Biochemical ◽  
Biochemical Profiles ◽  
Apparently Healthy

The cross-sectional study was carried out to compare the hematological and serum biochemical profiles between bovine tuberculosis (bTB) affected and apparently healthy cattle during the period from March 2014 to October 2016. A total of 189 blood samples were collected from 93 bTB affected and 96 apparently healthy cattle of 3 Upazilas of Chittagong. The bovine tuberculosis (bTB) was diagnosed primarily by Caudal Fold Tuberculin Tests (CFTT) and confirmed by the Comparative Intradermal Tuberculin Test (CITT). The whole blood was analyzed for hematology, and serum samples were used for biochemical analysis. The study revealed that, the values for TEC, Hb and PCV were higher in infected cohort (based on CFTT) in compare to their counterpart control group. Among these three parameters only PCV values were statistically significant (P ≤ 0.05). Only basophil percentage was significantly (P ≤ 0.05) higher in infected cohort. The values of total protein, albumin, creatinine and SGOT were insignificantly lower and the values of calcium & phosphorus were also insignificantly higher in bTB affected cattle than control group of cattle.

Immunologic Assessment of Patients Treated with Bovine Fibrin as a Hemostatic Agent

1996 ◽  
Vol 76 (06) ◽  
pp. 0925-0931 ◽  
Author(s):  
John F Carroll ◽  
Keith A Moskowitz ◽  
Niloo M Edwards ◽  
Thomas J Hickey ◽  
Eric A Rose ◽  
...  
Keyword(s):  
Coagulation Factors ◽  
Allergic Reactions ◽  
Factor V ◽  
Normal Range ◽  
Serum Samples ◽  
Human Fibrinogen ◽  
Bovine Thrombin ◽  
Thrombotic Complications ◽  
The Mean ◽  
Clinically Significant

SummaryTwenty-one cardiothoracic surgical patients have been treated with fibrin as a topical hemostatic/sealing agent, prepared from bovine fibrinogen clotted with bovine thrombin. Serum samples have been collected before treatment with fibrin and postoperatively between 1 and 9 days, 3 and 12 weeks, and 6 and 8 months. The titers of anti-bovine fibrinogen antibodies, measured by ELISA specific for immunoglobulins IgG or IgM, increased to maximal values after about 8 or 6 weeks, respectively. After 8 months, IgG titers were on average 20-fold lower than the mean maximal value, while IgM titers returned to the normal range. IgG was the predominant anti-bovine fibrinogen immunoglobulin as documented by ELISA, affinity chromatography and electrophoresis. Anti-bovine fibrinogen antibodies present in patients reacted readily with bovine fibrinogen, but did not cross-react with human fibrinogen as measured by ELISA or by immunoelectrophoresis. A significant amount of antibodies against bovine thrombin and factor V has been found, many cross-reacting with the human counterparts. No hemorrhagic or thrombotic complications, or clinically significant allergic reactions, occurred in any patient, in spite of antibody presence against some bovine and human coagulation factors. The treatment of patients with bovine fibrin, without induction of immunologic response against human fibrinogen, appeared to be an effective topical hemostatic/sealing measure.

Effects of X-Ray Exposure on Some CMI Regulatory Cytokines in Technicians Serum Samples

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Weam S Al-Hamadany
Keyword(s):  
Clinical Status ◽  
Cell Mediated Immunity ◽  
Normal Range ◽  
Serum Samples ◽  
X Ray ◽  
Immunosuppressive Factor ◽  
Elisa Technique ◽  
Immunological Process ◽  
Field Workers ◽  
Tomography Scan

Ionizing radiation considered as an immunosuppressive factor upon over dose of exposure. Radiation field workers usually following a periodic checkup to monitor changes in their clinical status. Cell Mediated Immunity (CMI) has an important cytokines that regulate this specific immunological process. This study estimated the Interleukins (IL-2, IL-12 and IL-18) levels in serum samples using ELISA technique. Serum samples were collected from X-ray Technicians (Radiography, Fluoroscopy and Computed Tomography Scan Technicians) working in AL-Muthanna governorate hospitals. A total of (60) technicians and (30) control were involved in this research. Results showed significant decrease in IL-2 levels and increase in IL-18 levels in test groups (technicians) as compared with controls. While, IL-12 levels did not show a difference; all obtained values were within normal range. Overdose of X-ray exposure caused CMI suppression via disturbing the levels of critical cytokines (IL-2 and IL-18) leading to CMI loss regulation.

scholarly journals Detection of Nipah virus in Pteropus medius in 2019 outbreak from Ernakulam district, Kerala, India

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. B. Sudeep ◽  
Pragya D. Yadav ◽  
Mangesh D. Gokhale ◽  
R. Balasubramanian ◽  
Nivedita Gupta ◽  
...  
Keyword(s):  
Index Case ◽  
Nipah Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Genotype I ◽  
Visceral Organs ◽  
Distinct Cluster ◽  
New Genotype ◽  
Kerala State ◽  
Set Up

Abstract Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection.

Normal serum biochemical and hematological parameters in Macaca fascicularis

1991 ◽  
Vol 20 (7) ◽  
pp. 345-351 ◽  
Author(s):  
Gemma Perretta ◽  
Anneo Violante ◽  
Manuela Scarpulla ◽  
Marta Beciani ◽  
Vincenzo Monaco
Keyword(s):  
Macaca Fascicularis ◽  
Hematological Parameters ◽  
Normal Serum ◽  
Serum Biochemical

scholarly journals Effect of Age and Abomasal Puncture on Peritoneal Fluid, Hematology, and Serum Biochemical Analyses in Young Calves

2005 ◽  
Vol 19 (6) ◽  
pp. 899-904 ◽  
Author(s):  
Luiz Claudio N. Mendes ◽  
Juliana R. Peiró ◽  
Francisco Leydson F. Feitosa ◽  
Maria Cecilia R. Luvizotto ◽  
Alexandre S. Borges ◽  
...  
Keyword(s):  
Peritoneal Fluid ◽  
Serum Biochemical ◽  
Biochemical Analyses ◽  
Effect Of Age

scholarly journals Hematological abnormalities in HIV infected individuals in correlation to CD4 counts and ART status

2016 ◽  
Vol 7 (4) ◽  
pp. 14-18 ◽  
Author(s):  
R Raman Thulasi ◽  
D Manimaran ◽  
G Hemanathan ◽  
Tameem Afroz ◽  
Radha Sagar
Keyword(s):  
Opportunistic Infections ◽  
Hematological Parameters ◽  
Cd4 Count ◽  
Health Concern ◽  
Medical Sciences ◽  
Cd4 Counts ◽  
Medical Centers ◽  
Hematological Abnormalities ◽  
Set Up ◽  
One Year

Background: HIV is pandemic and remains as a public health concern for many decades. This infection though associated with many opportunistic infections and neoplasms, it is further complicated with marked hematological abnormalities. The aim of this study is to determine the magnitude & severity of hematological abnormalities in HIV infected individuals and also to analyze these abnormalities in correlation with the CD4 counts. We also compared these hematological abnormalities in patients on ART and those not on ART.Materials and Methods: The study was conducted for a period of one year, on 120 HIV positive cases including both patients on ART & not on ART. Controls with similar age and sex distribution was set up. The blood samples were collected and processed in an automated cell counter. The parameters were tabulated and analyzed with respect to CD4 count & ART status.Results: Among the total of 120 HIV cases, 77% had anemia, 21% had leucopenia and 5% had thrombocytopenia. The magnitude and severity of anemia, leucopenia, thrombocytopenia and other parameters was found to be more in patients not on ART, when compared to patients on ART. Similarly, the magnitude and severity of most of hematological abnormalities were inversely proportional to the CD4 count in non-ART cases but not with cases on ART.Conclusion: The basic hematological parameters can be used as a prospective screening test to assess the severity and progression of HIV infection when CD4 count is not available. These parameters can also be used to assess the response to anti-retroviral treatment. Therefore, these basic hematological investigations readily available at all medical centers are of great use while treating HIV infected patients.Asian Journal of Medical Sciences Vol.7(4) 2016 14-18 

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