Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

Laxmi Shrestha; Amit Shrivastava; Bishal Joshi; Buddhi Raj Pokhrel; Shanti Gurung; Nabin Rayamajhi; Narayan Gautam

doi:10.3126/jucms.v8i02.34284

Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v8i02.34284 ◽

2020 ◽

Vol 8 (02) ◽

pp. 35-39

Author(s):

Laxmi Shrestha ◽

Amit Shrivastava ◽

Bishal Joshi ◽

Buddhi Raj Pokhrel ◽

Shanti Gurung ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Aqueous Extract ◽

Nigella Sativa ◽

Negative Control ◽

Hypoglycemic Effect ◽

Control Group ◽

Oral Glucose ◽

Standard Group

INTRODUCTION Diabetes mellitus is the most common metabolic disease worldwide. Nigella sativa also known as black cumin, is an emerging miracle herb with a rich historical and religious background. In the present study, we have attempted to study the hypoglycemic effect of aqueous extract of Nigella sativa on diabetes-induced rats. MATERIAL AND METHODS This preclinical study was conducted in Department of Pharmacology from August to October 2019. In this study, hyperglycemia was induced by a single intraperitoneal dose of 60 mg/kg streptozotocin in 0.1 M citrate buffer (pH 4.5). Animals were divided into five groups containing normal control, negative control, standard, test-1, and test-2. The aqueous extract of Nigella sativa was administered 400 and 800 mg/kg per oral in both the test groups for 18 days. Blood glucose level was measured at three-days interval by glucometer. Oral glucose tolerance test was done by administrating 2 gm/kg per oral glucose and the blood glucose was recorded every two-hours interval for eight hours. RESULTS An oral glucose tolerance test showed a decrease in blood glucose level at the dose of 400 and 800 mg/kg compared to the negative control group. The administration of aqueous extract of Nigella sativa for 18 days showed significant decrease in blood glucose level (p=0.001) compared to negative control group. The blood glucose level in test 1 (400 mg/kg) was significantly reduced compared to the standard group (p***<0.001). The blood glucose level in test 2 at 18th day was less significant (p*< 0.05) compared with standard group and test 1 (p**<0.01, p***<0.001). CONCLUSION The findings of the present study indicate that the aqueous extract of Nigella sativa has significant hypoglycemic effect in Wistar rats.

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Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3490 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

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Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

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PENGARUH EKSTRAK ETANOLIK BUAH PARIJOTO (Medinilla speciosa Blume) TERHADAP KADAR GULA DARAH DAN FUNGSI SEKSUAL TIKUS JANTAN GALUR WISTAR MODEL DIABETES MELLITUS KRONIK

JIFFK : Jurnal Ilmu Farmasi dan Farmasi Klinik ◽

10.31942/jiffk.v15i2.2558 ◽

2018 ◽

Vol 15 (2) ◽

pp. 1

Author(s):

Rina Wijayanti ◽

Asih Puji Lestari

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Sexual Function ◽

Glucose Level ◽

Ethanolic Extract ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Negative Group ◽

One Way Anova

ABSTRACTChronic diabetes mellitus is a disease of increased blood glucose levels in a long time so that it can affect sexual function such as damage spermatogenesis and aphrodisiac function. Parijoto has flavonoid content. This study aimed to determine the effect of ethanolic extract of parijoto (Medinilla speciosa Blume) fruit in blood glucose level and the sexual function in alloxan-induced diabetic male rats. The design of the study was post test only control group design, 25 male wistar rats aged 2 months with BB 100-200 gram divided into 5 groups. Group 1 (normal control), group 2 (negative control) induced alloxan 100mg / kgBB, groups 3, 4, and 5 were alloxan induced and administered EEBP at doses of 100 mg / kgBW, 250 mg / kgBW, and 500 mg / kgBW. Alloxan-induced rats were left for 4 weeks to achieve chronic DM. Data analysis of blood glucose level and spermatozoa quality using one way anova followed by LSD post hoc. While aphrodisiac of introducing and climbing using one way anova continued by LSD and coitus using kruskal wallis. The results showed that there were significant differences between negative control with normal group, III, IV, and V. The effect of ethanolic extract of parijoto fruit on blood glucose level and sexual function in alloxan-induced diabetic male rats showed no difference between treatment group of 100 mg / kgBW with negative group, and there were significant differences between treatment group of 250 mg / kgBW and 500 mg / kgBW with negative group. Ethanolic extract of parijoto fruit at dose 250 mg / kgBB and 500 mg / kgBB decrease blood glucose level, improve and increase sexual function in alloxan-induced diabetic male rats.

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Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

Nusantara Bioscience ◽

10.13057/nusbiosci/n090312 ◽

2017 ◽

Vol 9 (3) ◽

pp. 312-317

Author(s):

KARTIAWATI ALIPIN ◽

EMA PURNAMA SARI ◽

MADIHAH MADIHAH ◽

TIA SETIAWATI ◽

NINING RATNINGSIH ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Positive Control ◽

Negative Control ◽

Kidney Damage ◽

Diabetic Rat ◽

Control Group ◽

Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level â‰¥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

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PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculata) TERHADAP KADAR GLUKOSA DARAH MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN (Effect of Long Bean (Vigna unguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3983 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Vigna Unguiculata ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water

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Efektifitas Pemberian Minyak Zaitun Ekstra Virgin Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Dengan Induksi Hiperglikemia

Interest : Jurnal Ilmu Kesehatan ◽

10.37341/interest.v7i2.25 ◽

2018 ◽

Vol 7 (2) ◽

Author(s):

Sri Lestari Dwi Astuti ◽

Yeni Tutu Rohimah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Olive Oil ◽

Glucose Level ◽

Virgin Olive Oil ◽

Extra Virgin Olive Oil ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Standard Diet

Background: The global population of diabetes mellitus tends to increase every year. This is related to the increased of population numbers, lifestyle, increased prevalence of obesity and less physical activity (Smeltzer & Bare, 2002). Olive oil is a functional diet because it is rich of monounsaturated fatty acids (MUFA), especially oleic acid (70% - 80%), contains antioxidants and polyphenol compounds. Polyphenols are α-amylase enzyme inhibitors that have function in the breakdown of carbohydrates, so that the level of blood glucose in hyperglycemia can be lowered (Astrawan made, Wresdiyati Tutik, Nasution Nurlayla Amas, 2015). The goal of this study is to examine the effectiveness of extra virgin olive oil to blood glucose level. Method:This study used experimental laboratory study with post test only control group design. The independent variable was the extra virgin olive oil, while the dependent variable was the blood glucose level. The samples were 32 healthy 8 weeks old white male wistar strain rats with the weigh between 180 mg - 200 mg. It divided into 3 groups, the negative control group was fed with standard diet, the positive control group was fed with standard diet and 1 ml fructose for 4 weeks, the treatment group was fed with standard diet, 1 ml fructose and 0.5 gr extra virgin olive oil for 4 weeks, on the fifth week the fructose was increased into 4 ml/day for the next 4 weeks, on the 52nd day the blood glucose level was checked after it was fasted for 6 hours. The data analysis used t-test and Mann Withney test with α = 0.05. Result: The blood glucose level average of the positive control group was 309.57 ± 120.12, the blood glucose level average of the negative control group was 111.85 ± 10.57, the blood glucose level average of the treatment group was 115.12 ± 12.25. Conclusion: Giving the extra virgin olive oil can effectively lower the blood glucose level on the male wistar rats.

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Effect of administration of Ehretia anacua aqueous extract on blood glucose level in alloxan - induced diabetic rat

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.11.2.0068 ◽

2021 ◽

Vol 11 (2) ◽

pp. 001-009

Author(s):

Oyelade Waheed Abimbola ◽

Oyebode Joseph Ademola ◽

Fajilade Temilade Olawande

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Aqueous Extract ◽

Diabetic Rats ◽

Diabetic Rat ◽

Control Group ◽

Albino Rats ◽

Group I ◽

Histopathological Studies

The effects of crude aqueous extract of Ehretia anacua on alloxan induced diabetic rats was investigated. Male albino rats of weighing between 120 to 150 were used, divided into 6 groups of five animals per group. Group I received distilled water throughout of the experiment and served as the control. Group II received 110 mg/kg of alloxan interperitoneally. Groups III, IV, V and VI received 110 mg/kg of alloxan and in addition administered with aqueous Ehretia anacua extract daily for 14 days. Blood glucose level was monitored at five days interval for fourteen days. Target organs (pancrease) was taken from each rat. The histopathological studies of the pancrease were examined. In alloxan - induced diabetic rats, blood glucose level was significantly increased compared with the control rats. Treating diabetic rats with 50, 100 and 200 mg/kg bw Ehretia anacua caused a significant decrease in the blood glucose level. The Photomicrograph of the histopathology examination of the pancrease (× 100) of the groups treated with alloxan showed poor architecture was destroyed whereas those treated with Ehretia nancua showed normal architecture. This illustrates the amelliorative effects of the extract on the alloxan-induced tocicity. It could be concluded from these results that, Ehretia nancua extract should be used in manufacture processes of the natural products as functional foods or as a dietary supplement with anti-diabecretic activity as hypoglycemic effect.

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COMPARISON OF ANTIHYPERGLYCEMIC ACTIVITY OF DIFFERENT PARTS OF KLUTUK BANANA (MUSA BALBISIANA COLLA)

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021.v13s3.12 ◽

2021 ◽

pp. 57-61

Author(s):

TITA NOFIANTI ◽

AHMAD MUHTADI ◽

IRDA FIDRIANNY

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Negative Control ◽

Antidiabetic Activity ◽

Oral Glucose ◽

Musa Balbisiana ◽

Antihyperglycemic Activity ◽

Peel Extract ◽

Different Parts

Objective: Banana is a plant that grows in Indonesia and is widely consumed by Indonesian people. One of banana type is klutuk banana (Musa balbisiana Colla). There were many types of researches about klutuk banana for antidiabetic activity; however, antidiabetic activity of its peel and pulp are still unknown. Methods: The objective of the study was to determine of antihyperglycemic activity of different parts of klutuk banana. Animals were divided into 15 groups, namely normal control, negative control, positive control (glibenclamide 0.65 mg/kg body weight (bw), and 12 sample groups. All animals were given 2 g/kg bw glucose monohydrate and blood glucose level was measured every 30 min for 120 min. Results: The results of the oral glucose tolerance test (OGTT) showed that KPE2 (klutuk peel extract 350 mg/kg bw) gave higher activity to decrease blood glucose level compared to the other groups at the minute of 30 (-24.83%; p. 0.00), 60 (-33.93%; p. 0.000), 90 (-46.29%; p. 0.000) and 120 (-35.44%; p. 0.000). Conclusion: The klutuk peel extract has very strong antioxidant activity and antihyperglycemic activity at a dose of 350 mg/kg bw.

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Evaluation of the Hypoglycemic Potential of Leaves Extract of Spondias pinnata (L.f.) Kurz. from Nepal

The Scientific World JOURNAL ◽

10.1155/2021/3230351 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Kusum Sai ◽

Sumit Bahadur Baruwal Chhetri ◽

Shankar Raj Devkota ◽

Deepa Khatri

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Hypoglycemic Activity ◽

Hypoglycemic Agents ◽

Deciduous Tree ◽

Control Group ◽

Oral Glucose ◽

Phytochemical Screening ◽

Traditional Use

Spondias pinnata (L.f.) Kurz. (family: Anacardiaceae) is a wild deciduous tree indigenous to southeast Asian countries. Different parts of this plant are used traditionally for the treatment and cure of various disorders and illnesses. S. pinnata leaves are used to prevent and treat diabetes in traditional Balinese medicine. However, scientific study on the antihyperglycemic effect of its leaves has not been reported yet. Therefore, this study aims to perform phytochemical screening and investigate the hypoglycemic potential of S. pinnata leaves extract. Preliminary phytochemical screening of the hydroethanolic extract was performed following the standard tests. In vivo hypoglycemic activity of the leaves extract was evaluated using normal and glucose-loaded rats. The results displayed the presence of phytochemical constituents such as saponins, phenolic compounds, flavonoids, and terpenoids. S. pinnata (500 mg/kg) and metformin (100 mg/kg) exhibited a significant ( p < 0.05 ) decrease in blood glucose level at 1, 2, and 3 h in normal rats when compared to the control group. Metformin- (100 mg/kg)- and S. pinnata- (500 mg/kg)- treated groups showed a maximum decrease in the blood glucose level at 3 h after single-dose administration in the oral glucose tolerance test (OGTT). In conclusion, S. pinnata leaves possess a significant hypoglycemic activity in the animal model and thus support its traditional use to treat diabetes. Therefore, a detailed mechanism-based study and isolation of bioactive compounds from S. pinnata leaves would be beneficial in the future for the search of new hypoglycemic agents.

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Study of the oral hypoglycemic activity of Moringaoleifera leaves alone and in combination with Glibenclamide in streptozotocin induced diabetic albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20174378 ◽

2017 ◽

Vol 6 (10) ◽

pp. 2470

Author(s):

Sushma V. Naidu ◽

Suresha R. N. ◽

Jayanthi M. K. ◽

Satish A. M. ◽

Kalabharathi H. L. ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Diabetic Control ◽

Hypoglycemic Activity ◽

Control Group ◽

Albino Rats ◽

Standard Group ◽

Gum Acacia ◽

Diabetic Control Group

Background: Oringaoleifera is a widely used plant with high medicinal value, well known for its pharmacological actions and is used in various conditions. It has been reported to have many biological properties like anti-inflammatory, antimicrobial, antispasmodic, antitumour including antidiabetic activity.Methods: The study was carried out in Wistar albino rats with body weight 150-250gms. Diabetes was induced by injecting Streptozotocin intraperitoneally- dose 55 mg/kg BW. Animals were divided into 5 groups with 6 animals in each group. First group (Control) was given 2% gum acacia. Other 4 groups were induced diabetes by giving Streptozotocin. Diabetic control group received gum acacia (0.5 ml), Standard group received Glibenclamide (0.5mg/kg BW), Test group received Moringaoleifera extract (300mg/kg) and Test+ Standard group receiving combination of Moringaoleifera and glibenclamide at half the above doses. All drugs were given orally for 28 days and blood glucose levels analyzed using Glucometer on Day 0 before drug and on D1, D3, D7, D14, D21, and D28. Data were statistically analyzed by ANOVA and Tukey‘s Post Hoc test.Results: Hypoglycemia produced by Moringaoleifera extract was significant (p<0.001) when compared to diabetic control group from day 7 to day 28. The percent reduction of blood glucose level was 52.9% as compared to Glibenclamide group 61.3%. The combination group also showed significant hypoglycemic activity the percentage reduction being 56.44%.Conclusions: Thus, Moringaoleifera decreased blood glucose level efficaciously as compared to diabetic control group and similar to standard group at p<0.001.

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