Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level â‰¥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

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Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3490 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

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Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

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Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2021-0151 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Solomon E. Owumi ◽

Jeremiah O. Olugbami ◽

Andrew O. Akinnifesi ◽

Oyeronke A. Odunola

Keyword(s):

Blood Glucose ◽

Wistar Rats ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Telfairia Occidentalis ◽

Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

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PENGARUH PEMBERIAN KACANG PANJANG (Vigna unguiculata) TERHADAP KADAR GLUKOSA DARAH MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN (Effect of Long Bean (Vigna unguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3983 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Intan Fitri Aprila ◽

M. Nur Salim ◽

Razali Daud ◽

T. Armansyah ◽

Nuzul Asmilia ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Vigna Unguiculata ◽

Mus Musculus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water

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Uji efek air perasan albedo semangka kuning (Citrilus lanatus (Thunb.) Matsum. & Nakai) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.12149 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Savitri Muhammad Nur ◽

Henoch Awaloei ◽

Jane Wuisan

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

White Layer ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Abstract: Indonesian has various natural resources, second as largest after Brazil. One of the traditional medicine that has been used is watermelon. The part of watermelon that is known to be used as medicine is its white layer between the peel and the fruit, albedo. This study aimed to determine the effect of yellow watermelon albedo juice on blood glucose levels in wistar rats induced by alloxan. Subjects consisted of 18 male Wistar rats divided into 6 groups as follows: a negative control group, 3 groups of hyperglycemic rats induced with alloxan 120 mg/kg body weight; and 2 groups given only yellow watermelon albedo juice. Hyperglycemic rats were given yellow watermelon albedo with dose of 9 g/kg body weight and 4,5 g/kg body weight; positive control group was given novomix; and two other groups were given only yellow watermelon albedo juice. Blood glucose levels of each rat were evaluated on day 1, day 2, and day 3 before the treatment after 6 hours, 12 hours, 18 hours, 24 hours. The results showed that administration of yellow watermelon albedo juice 9 g/kg body weight of Wistar rats reduced the blood glucose level until 126.67 mg/dl, meanwhile the dose 4.5 g/kg body weight reduced the blood glucose level until 173.67 mg/dl. Conclusion: Administration of yellow watermelon albedo juice of 9 g/kg body weight and 4.5 g/kg body weight could reduce blood glucose levels of hyperglycemic Wistar rats induced by alloxan. Keywords: yellow watermelon albedo, hyperglycemia, blood glucose level Abstrak: Indonesia merupakan negara yang memiliki keanekaragaman hayati terkaya kedua didunia setelah Brazil. Salah satu obat tradisional yang digunakan ialah buah semangka. Bagian dari semangka yang digunakan ialah lapisan yang berwarna putih yakni antara kulit dan daging buah semangka disebut albedo. Penelitian ini bertujuan untuk menguji pengaruh pemberian air perasan albedo semangka kuning terhadap kadar glukosa darah pada tikus wistar yang diinduksi aloksan. Subjek penelitian yaitu 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok, yaitu: kelompok kontrol negatif; 3 kelompok tikus Wistar hiperglikemik yang diinduksi aloksan dengan dosis 120 mg/kg BB; dan 2 kelompok tikus Wistar yang hanya diberikan albedo semangka kuning. Kelompok tikus yang hiperglikemik diberi albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB; kelompok kontrol positif diberikan novomix; dan 2 kelompok hanya diberikan albedo semangka kuning. Pemeriksaan kadar glukosa darah dari semua kelompok tikus Wistar dilakukan pada hari ke-1, hari ke-2, dan hari ke-3 pada jam ke 0, 6, 12, 18, dan 24. Hasil penelitian memperlihatkan pemberian air perasan albedo semangka kuning dosis 9 g/kg BB dapat menurunkan kadar glukosa darah sebanyak 126,67 mg/dl sedangkan dosis 4,5 g/kg BB sebanyak 173,67 mg/dl. Simpulan: Air perasan albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB dapat menurunkan kadar glukosa darah tikus Wistar hiperglikemik yang diinduksi aloksan.Kata kunci: albedo semangka kuning, hipeglikemi, kadar glukosa darah

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Anti-diabetic activity of diphenhydramine in diabetic rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3102 ◽

2020 ◽

Vol 11 (4) ◽

pp. 5067-5070

Author(s):

Pang Jyh Chayng ◽

Nurul Ain ◽

Kaswandi Md Ambia ◽

Rahim Md Noah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Insulin Level ◽

Diabetic Rats ◽

Group Iv ◽

Diabetic Rat ◽

Control Group ◽

Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p<0.05) and group IV (11.34 ±3.67, p<0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p<0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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UJI PERBANDINGAN EFEKTIVITAS ANTIDIABETES EKSTRAK POLISAKARIDA DAN SENYAWA POLIFENOL ALGA COKLAT (Sargassum sp.) PADA MENCIT YANG DIINDUKSI ALOKSAN

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v4i1.157 ◽

2018 ◽

Vol 4 (1) ◽

pp. 48

Author(s):

Agung Giri Samudra ◽

Fathur Sani K ◽

Moniq Chintama

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Brown Algae ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Group Iii ◽

Percentage Decrease ◽

Polyphenolic Extract

Diabetes is one of the most common diseases in the world. So there is an effort to prevent or treat the disease. As brown algae (Sargassum sp.) Contains polysaccharides that regulate sugar intake in the body, and polyphenols act as antioxidants that can stimulate insulin secretion in pancreatic β cells. This study aims to determine the effectiveness of Antidiabetes Polysaccharide Extract and Brown Algae Polifenol Compounds (Sargassum sp) On Alloxaninduced Mice. The test animals were grouped into 4 groups I (CMC 1%), Group II (Glibenclamide 0.52mg / 20grBB), Group III (5mg / 20grBB polyphenol extract), Group IV (5mg / 20grBB polysaccharide extract). Blood glucose was measured using Easy touch glucometer on days 0, 3, 7, 14, 21, and 28. Analyze data using one-way ANOVA, followed by LSD test. The percentage decrease in blood glucose level in positive control, polyphenol extract and polysaccharide were 42,86%, 58,12%, and 54,20%. Statistically, the polyphenolic extract and the brown algae polysaccharide lower the blood glucose level significantly compared with the negative control (p≤0,05). Decreased blood glucose in polyphenol extracts and polysaccharides showed statistically significant differences

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Aktivitas Antihiperglikemik dari Ekstrak Etanol dan Heksana Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) pada Tikus Wistar (Rattus norvegicus L.) yang Hiperglikemik

Jurnal MIPA ◽

10.35799/jm.2.2.2013.2999 ◽

2013 ◽

Vol 2 (2) ◽

pp. 109

Author(s):

Sariyana Togubu ◽

Lidya I. Momuat ◽

Jessy E. Paendong ◽

Navila Salma

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Ethanol Extract ◽

Tolerance Test ◽

Negative Control ◽

Hexane Extract ◽

Test Method

Telah dilakukan penelitian kadar glukosa darah tikus wistar yang hiperglikemia pada pemberian ekstrak etanol dan heksana tumbuhan suruhan (Peperomia pellucida [L]. Kunth). Penelitian ini menggunakan metode uji toleransi glukosa, terhadap 16 ekor tikus jantan wistar yang hiperglikemia akibat diinduksi sukrosa. Selanjutnya, tikus dibagi dalam 4 kelompok perlakuan yaitu kelompok kontrol negatif (K-) diberi CMC 0,5%, kelompok kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok ekstrak etanol tumbuhan suruhan (EETS) dosis 40 mg/kgBB, dan kelompok ekstrak heksana tumbuhan suruhan (EHTS) dosis 40 mg/kgBB. Setiap kelompok terdiri dari 4 ekor tikus. Kadar glukosa darah tikus diukur dengan alat Drglukometer pada menit ke-30, 60, dan 120 setelah diberikan perlakuan. Hasil penelitian menunjukkan bahwa pada menit ke-120, kadar glukosa darah tikus pada kelompok EETS dan EHTS masing-masing turun sebesar 54,57% dan 51,25%, dan tidak berbeda nyata dengan K(+). Kadar glukosa darah kelompok K(+) dan EETS pada menit ke-120 mencapai kadar yang sama dengan keadaan basal (normal). Penelitian ini menyimpulkan bahwa ekstrak etanol dan heksana tumbuhan suruhan dengan dosis 40 mg/KgBB memiliki efek hipoglikemik pada tikus jantan wistar yang hiperglikemia.A study on blood glucose level in hyperglycemic wistar rats treated with ethanol and hexane extracts of suruhan (Peperomia pellucida [L]. Kunth) had been done. This study used glucose tolerance test method applied on 16 male wistar rats which were hyperglycemic by sucrose induction. The rats were divided into four treatment groups, each of which contained four rats, which were negative control (K-) group treated with CMC 0.5%, positive control (K+) group treated with glibenclamide 0.45 mg/kg body weight, group treated with ethanol extract of P. pellucida (EETS) 40 mg/kg body weight, and group treated with hexane extract of P. pellucida (EHTS) 40 mg/kg body weight. Glucose level in rat blood was measured using Drglucometer at minute 30, 60, and 120 after treatment. The results showed that at minute 120 the blood glucose level of EETS and EHTS was decreased by 54.57% and 51.25%, respectively, which were not significantly different from K(+). At minute 120, blood glucose of K(+) and EETS reached the same level with that of basal (normal) state. It was concluded that ethanol and hexane extract of P. pellucida 40 mg/kg body weight had hypoglycemic effect on hyperglycemic wistar rats.

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Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v8i02.34284 ◽

2020 ◽

Vol 8 (02) ◽

pp. 35-39

Author(s):

Laxmi Shrestha ◽

Amit Shrivastava ◽

Bishal Joshi ◽

Buddhi Raj Pokhrel ◽

Shanti Gurung ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Aqueous Extract ◽

Nigella Sativa ◽

Negative Control ◽

Hypoglycemic Effect ◽

Control Group ◽

Oral Glucose ◽

Standard Group

INTRODUCTION Diabetes mellitus is the most common metabolic disease worldwide. Nigella sativa also known as black cumin, is an emerging miracle herb with a rich historical and religious background. In the present study, we have attempted to study the hypoglycemic effect of aqueous extract of Nigella sativa on diabetes-induced rats. MATERIAL AND METHODS This preclinical study was conducted in Department of Pharmacology from August to October 2019. In this study, hyperglycemia was induced by a single intraperitoneal dose of 60 mg/kg streptozotocin in 0.1 M citrate buffer (pH 4.5). Animals were divided into five groups containing normal control, negative control, standard, test-1, and test-2. The aqueous extract of Nigella sativa was administered 400 and 800 mg/kg per oral in both the test groups for 18 days. Blood glucose level was measured at three-days interval by glucometer. Oral glucose tolerance test was done by administrating 2 gm/kg per oral glucose and the blood glucose was recorded every two-hours interval for eight hours. RESULTS An oral glucose tolerance test showed a decrease in blood glucose level at the dose of 400 and 800 mg/kg compared to the negative control group. The administration of aqueous extract of Nigella sativa for 18 days showed significant decrease in blood glucose level (p=0.001) compared to negative control group. The blood glucose level in test 1 (400 mg/kg) was significantly reduced compared to the standard group (p***<0.001). The blood glucose level in test 2 at 18th day was less significant (p*< 0.05) compared with standard group and test 1 (p**<0.01, p***<0.001). CONCLUSION The findings of the present study indicate that the aqueous extract of Nigella sativa has significant hypoglycemic effect in Wistar rats.

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