scholarly journals Formulation and Evaluation of Antimicrobial Herbal Ointment

1970 ◽  
Vol 6 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Himal Paudel Chhetri ◽  
Nisha Shrestha Yogol ◽  
Jyoti Sherchan ◽  
Anupa KC ◽  
S Mansoor ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Dosage Forms ◽  
Herbal Drugs ◽  
Zone Of Inhibition ◽  
Klebsiella Species ◽  
Effective Combination ◽  
Engineering And Technology ◽  
Ethanolic Extracts ◽  
Micro Organisms

Most of the antibiotics were originally derived from micro-organisms while thechemotherapeutic agents are from plants. Herbal medicine refers to the use of anyplant's seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. Alongwith other dosage forms, herbal drugs are also formulated in the form of ointment. Anointment is a viscous semisolid preparation used topically on a variety of bodysurfaces. The objective of the study was to formulate and evaluate the antimicrobialherbal ointment from the local medicinal plants. The ethanolic extracts of the selectedplants were taken in different ratio randomly and the antimicrobial tests of thecombinations were carried out. The most effective combination was then determinedby comparing the results of the zone of inhibition given by the 10 different extractratios on Staphylococcus aureus, Escherichia coli and Klebsiella species. Then theminimum inhibitory concentration of the effective combination was found out. Theointment base was prepared and formulation of ointment was done by incorporatingthe active ingredients in most effective ratio in the base by trituration. After thecompletion of the formulation, quality of the ointment was assessed in terms ofirritancy, spreadability, diffusion and stability.Key words: Herbal ointment; minimum inhibitory concentration; irritancy;spreadability; diffusion; stabilityDOI: 10.3126/kuset.v6i1.3317 Kathmandu University Journal of Science, Engineering and Technology Vol.6(1) 2010, pp102-107

scholarly journals Phytochemical and Antimicrobial Evaluations of some Medicinal Plants of Nepal

1970 ◽  
Vol 4 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Himal Paudel Chhetri ◽  
Nisha Shrestha Yogol ◽  
Jyoti Sherchan ◽  
Anupa K.C. ◽  
S Mansoor ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Reducing Sugar ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Klebsiella Species ◽  
Engineering And Technology ◽  
And Staphylococcus Aureus

Use of plant based drugs and chemicals for curing various ailments and personal adornment is as old as human civilization. Plants and plant-based medicaments are the basis of many of the modern pharmaceuticals we use today for our various ailments. The aim of the study was to find out the bioactive chemical constituents and to evaluate the antimicrobial activity of the ethanolic extract of traditionally used eight medicinal plants of Nepal. A qualitative phytochemical analysis was performed for the detection of alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins and reducing sugar. The highest yield of ethanolic extract was found in Azadiracta indica (29.08%). Ocimum santrum contained all the chemicals except flavonoids and reducing sugar however the Colquhounia coccinea lacked alkaloids and reducing sugar. The antimicrobial activities of these plants extract were also observed. The extract of Rhododendron setosum and the essential oil of Eucalyptus globulus were most effective against Escherichia coli and Staphylococcus aureus respectively. But the extracts of Azadiracta indica and Elsholtzia fructicosa were found to be most effective against Klebsiella species. Key words: Phytochemical screening; plant extracts; zone of inhibition DOI: 10.3126/kuset.v4i1.2883 Kathmandu University Journal of Science, Engineering and Technology Vol.4, No.1, September 2008, pp 49-54

scholarly journals Antimicrobial Activity of Ethanolic Extract of Medicinal Plants against Human Pathogenic Bacteria

2018 ◽  
Vol 5 ◽  
pp. 1-6
Author(s):  
Hira Sakha ◽  
Rejila Hora ◽  
Shilpa Shrestha ◽  
Shreeya Acharya ◽  
Dinesh Dhakal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Human Pathogenic Bacteria ◽  
Neem Leaves ◽  
Ethanolic Extracts

Objectives: To evaluate the antimicrobial activity of medicinal plants against human pathogenic bacteria and perform Minimum Inhibitory Concentration (MIC) of plants extracts. Methods: Rhizome of Curcuma longa, dried buds of Synzygium aromaticum, seeds of Zanthoxylum armatum and leaves of Elaeocarpus ganitrus, Psidium guajava, Azadirachta indica, and Artemisia vulgaris were collected from hilly regions of Nepal. The plant parts were air-dried at room temperature for several days and grinded to powder form. The ethanolic extracts of medicinal plants were prepared by using the percolation process of extraction using separating funnel and tested against human pathogenic bacteria by disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) of the plant extracts were performed. Results: All plants extracts exhibited antibacterial properties against bacteria under study. However, extract from S. aromaticum (Clove), P. guajava (Guava) and E. ganitrus (Rudraksh) leaves showed most promising result against Staphylococcus aureus with zone of inhibition of 14mm, 16mm and 16 mm respectively. Likewise, S. aromaticum (Clove), C. longa (Turmeric) and P. guajava (Guava) showed good antibacterial activity against Escherichia coli with zone of inhibition of 11mm, 11mm and 10mm respectively. A. vulgaris (Titepati) and A. indica (Neem leaves) showed promising activity against Pseudomonas aeruginosa with zone of inhibition of 11mm. Z. armatum (Timur) showed good result against E. coli with zone of inhibition 10mm. MIC values of ethanolic extracts of S. aromaticum and E. ganitrus were found to be at the range of 12.5-25mg/ml. Conclusion: This study has helped to understand the use of these plants as traditional medicine in an economic and safe alternative to treat infectious diseases.

Comparative Pharmacognostic, Physicochemical and Phytochemical Studies of Different Samples of Plumbago zeylanica L. Roots

2017 ◽  
Vol 9 (06) ◽  
Author(s):  
Neha Jain ◽  
Mohan Lal Kori
Keyword(s):  
Phytochemical Analysis ◽  
Local Market ◽  
Herbal Drugs ◽  
Disease Treatment ◽  
Organoleptic Evaluation ◽  
Plumbago Zeylanica ◽  
Qualitative And Quantitative ◽  
Crude Drugs ◽  
Phytochemical Studies

The objective of the present study is to evaluate the quality of the marketed and self collected samples of Plumbago zeylanica L. roots on the standardization parameters. This study is planned mainly to confirm changes with quality of drug. Now a day’s more demand of herbal drugs for disease treatment, lack of knowledge of proper methodology and availability are promoting the practices of adulteration and substitution. Thus, the standardization of the plant crude drugs is necessary to maintain their therapeutic efficacy. Comparative studies were carried out to evaluate the standards of P. zeylanica L. with emphasis on organoleptic evaluation, physicochemical and phytochemical analysis. Samples were procured from local market and self collected to determine the qualitative and quantitative variations. The result indicates that self collected sample showed significant results with comparison to marketed sample.

Microbial Quality of Human Wastes and Treatment Plant Effluent

1986 ◽  
Vol 18 (7-8) ◽  
pp. 257-265 ◽  
Author(s):  
K. Kawamura ◽  
M. Kaneko
Keyword(s):  
Staphylococcus Aureus ◽  
Vibrio Cholerae ◽  
Treatment Plant ◽  
Microbial Quality ◽  
Chlorination Process ◽  
Fecal Streptococci ◽  
Human Wastes ◽  
Micro Organisms ◽  
Spore Forming Bacteria

In order to evaluate the microbial quality of human wastes and effluents from treatment processes, the microbial flora of samples was examined. Total coliforms, fecal coliforms and fecal streptococci were used as indicator micro-organisms, and Vibrio cholerae non O-1, Salmonella, Staphylococcus aureus and Welchii (Clostridium perfrigens) were selected as pathogenic organisms. Salmonella was detected in only the water samples from the night soil treatment plant, while Staphylococcus aureus was detected in the night soil and the samples from the night soil purification tanks. Vibrio cholerae non 0-1 was not detected in any samples, but Welchii existed in almost all samples. Generally, the density levels and distribution patterns of the indicator micro-organisms were similar to those in the raw wastewaters. The microbial flora was not changed remarkably after the primary sedimentation process and the biological treatment process. After the chlorination process, total colonies, spore-forming bacteria, Welchii, moulds and fecal streptococci could survive, and spore-forming bacteria formed the majority of the total colonies in the well-chlorinated effluents. Welchii at the level of 103/100 ml can be used as the indicator micro-organism to ensure a sanitary safe discharge, because it can survive at the level of 103−104/100 ml even if other indicators and pathogenic micro-organisms are inactivated completely by the chlorination process.

The real concept of substitution in Ayurveda literature and adulteration the misleading concept of modern era

2018 ◽  
Vol 3 (3) ◽  
Author(s):  
Ashashri Shinde ◽  
Pankaj Gupta ◽  
Sudipt Rath
Keyword(s):  
Medicinal Plant ◽  
Plant Material ◽  
Genetic Parameters ◽  
Herbal Drugs ◽  
Material Identification ◽  
The Real ◽  
Physical Chemical ◽  
Unfair Trade ◽  
Modern Era

A quality drug is central to the success of any therapeutic plan. The quality of drug is determined right from the collection to delivery to the patients. The commonest problem involving the medicinal plant stating materials is intentional or unintentional substitution and adulteration owing to multiple reasons like unavailability, higher costs, unfair trade etc. This trend was also present in the olden days, as evident from the concept of substitute drugs (Pratinidhi Dravya) as available in Yogratanakara, Bhavaprakasha and Bhaishajyaratnawali. Therefore, Charka and later Acharyas also have dealt with authentication and standardization of herbal drugs and formulations in detail by using four Pramanas (tools of knowledge) Ch.Vi.8/87. Nowadays the concept of substitution is entirely converted into intentional and unintentional malpractices of adulteration. The established authenticity parameters for plant material identification and standardization like organoleptic, physical, chemical and genetic parameters are relatively inaccessible for routine use. Not withstanding the accuracy and usefulness of these lab parameters and delay in the development of easy to perform parameters for reasonable drug authentication. These adulteration malpractices spoils the market of herbal industries. In this article we discuss about concept of substitution in ancient Ayurveda and at present intentional and unintentional adulteration practices.

DRUG REGULATORY PROCEDURES IN TANZANIA: A GLIMPSE

2015 ◽  
Vol 3 (2) ◽  
pp. 1-7 ◽  
Author(s):  
Achin Jain ◽  
M P Venkatesh M P ◽  
Pramod T.M. Kumar
Keyword(s):  
Quality Control ◽  
Medical Devices ◽  
Emerging Market ◽  
Well Being ◽  
New Drugs ◽  
Regulatory Body ◽  
Herbal Drugs ◽  
Drug Products ◽  
Set Up

In Tanzania, Tanzania Food and Drugs Authority (TFDA), is a regulatory body responsible for controlling the quality,safety and effectiveness of food, drugs, herbal drugs, cosmetics and medical devices. The Authority has been ensuringsafety, efficacy and quality of medicines by quality control tests; in addition to other quality assessment mechanisms.The guidelines laid by TFDA have also emanated from commitment to democracy and gives strong emphasis to thefulfilment of the needs of the less privileged rural population.Tanzania is an emerging market; the pharmaceutical market is valued at over US$250 million, and is growing at anannual rate of around 16.5% and is expected to reach approximately US$550 billion in 2020. Currently, the market ishighly dependent on imports, which account for around 75% of the total pharmaceutical market.The procedures and approval requirements of new drugs, variations, import, export and disposal have been set up bythe TFDA, which help in maintaining quality of the drug products that are imported as well being produced locally 

scholarly journals Microbiological quality of non-sterile pharmaceuticals in Egypt

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nourhan Mahrous Ahmed Salem ◽  
Mohamed Abbas Elbarrawy ◽  
Nashwa Fawzy Abd El Moez Azzam
Keyword(s):  
Bacterial Growth ◽  
Stenotrophomonas Maltophilia ◽  
Microbiological Quality ◽  
Pseudomonas Stutzeri ◽  
Dosage Forms ◽  
Aeromonas Salmonicida ◽  
Acceptable Limit ◽  
Organoleptic Characteristics

Abstract Background The context and purpose of the study are as follows: Drug-borne infections may arise from non-adherence to strict microbiological quality of pharmaceuticals products. Moreover, presence of exceeding levels of microorganisms in non-sterile pharmaceuticals may lead to change of their organoleptic characteristics and loss of effectiveness. The aim of the study is to evaluate the microbiological quality of commonly used non-sterile pharmaceuticals in Alexandria, Egypt. Results Average microbiological quality of the studied products, where 17.03% and 19.23 % of samples had exceeded the maximum acceptable limit of TAMC and TYMC, respectively. No E. coli was isolated from oral products. None of S. aureus nor P. aeruginosa were isolated from topical products. Bacterial growth was recovered from 19 (10.44%) of the studied 182 samples, four Bacillus spp. had been recovered from topical products, two P. aeruginosa isolates were recovered from tablets and other two were isolated from syrups dosage forms. Other isolates were Pseudomonas stutzeri, Stenotrophomonas maltophilia, Acinetobacter Achromobacter denitrificans, Ochrobactrum anthropic and Aeromonas salmonicida. Conclusion Average microbiological quality of the tested pharmaceuticals used in Alexandria.

ANTIFUNGAL TOPICAL GEL OF LEAF EXTRACT OF AMARANTHUS VIRIDIS L. FOR TREATMENT OF CUTANEOUS CANDIDIASIS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 39-44
Author(s):  
A. P. Pandit ◽  
◽  
K. S. Khandagale ◽  
V. C Nakhate ◽  
N. N Dharmadhikari
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
High Viscosity ◽  
Good Choice ◽  
Zone Of Inhibition ◽  
Minimum Fungicidal Concentration ◽  
Topical Gel ◽  
Cutaneous Candidiasis

The objective of the study was to prepare antifungal gel using leaves of Amaranthus viridis for the treatment of cutaneous candidiasis. The leaves were studied for pharmacognostic evaluation. The powder of leaves was tested for phytoconstituents. The plant extract was evaluated for the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and antifungal activity. Gel was prepared and evaluated for pH, viscosity, homogeneity and grittiness. MIC and MFC of extract were both found to be 600 mg. The zone of inhibition of extract was obtained at 21.2±0.2mm, which confirmed antifungal activity, due to presence of phenolic compound. Gel exhibited good antifungal activity, good spreadability, extrudability and high viscosity. Thus, gel loaded with leaves extract of A. viridis is a good choice for the treatment of cutaneous candidiasis.

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