Antioxidative status as assessed by enzymatic activity in the tear fluid and by the levels of sulfur-containing protein groups in the blood and tear fluid of myopes before and after treatment with a thiol agent

2017 ◽  
Vol 68 (4) ◽  
pp. 31-39 ◽  
Author(s):  
E. Surova ◽  
◽  
I. Boichuk ◽  
S. Kolomiichuk ◽  
◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Tear Fluid ◽  
Antioxidative Status ◽  
Before And After ◽  
Sulfur Containing

Determination of Phenolic Compounds from Jimma Drinking Water Using 4-Aminoantipyrine

2020 ◽  
Vol 12 (5) ◽  
pp. 690-694
Author(s):  
Kassahun Dejene Belayneh ◽  
Khalid Siraj ◽  
Mengesha Tigist
Keyword(s):  
Drinking Water ◽  
Phenolic Compounds ◽  
Disease Transmission ◽  
Raw Water ◽  
Treated Water ◽  
Before And After ◽  
Sulfur Containing ◽  
Jimma Town

The quality of drinking water in Ethiopia is an influential environmental factor of health and water can use as a medium for disease transmission in countries on all continents; all are affected from the poorest to the richest. Moreover, in our globe millions of people are unprotected to unsafe concentration of chemical pollutants in the drinking water. Dichlorohenols are considered as key water pollutants that are destructive to creatures at stumpy concentrations and many of them have been clustered as detrimental pollutants because of their probable to damage human health even in low concentration. This research aimed to determine phenolic compounds from drinking water of Jimma town which is found south west of Ethiopia, by using 4-Aminoantipyridine (AAP). The sample of drinking water before and after treatment was collected for determination of pollutants which classified as raw water (RW), treated water (TW) and system distributed water (SDW). The sample of water was preserved by phosphoric acid and sulfuric acid under pH 4 and distillation was carried out in order to remove sulfur containing compounds by addition of CuSO4 solution. In the analysis of water using 4-Aminoantipyrine the treated water and the system distributed water have been contained 2.73 ppb and 3.64 ppb which had more phenolic compounds as compared to raw water of 1.18 ppb. Therefore, the existence of phenolic compound in both treated and system distributed water of Jimma town drinking water has more phenolic compounds as compared to the permissible level of both Ethiopian Standard, 2 ppb and EPA Standards, 1 ppb.

scholarly journals Estimation of lacrimal dysfunction indices in patients with recurrent pterygium

2020 ◽  
Vol 13 (1) ◽  
pp. 11-16
Author(s):  
Erkin N. Bilalov ◽  
Azamat F. Yusupov ◽  
Ahmadjon E. Nozimov ◽  
Okilkhon I. Oripov
Keyword(s):  
Tear Film ◽  
Tear Fluid ◽  
Fluid Composition ◽  
Optimal Correction ◽  
Number Of Patients ◽  
Before And After ◽  
Pathogenetic Therapy ◽  
Recurrent Pterygium ◽  
The Impact ◽  
Tear Dysfunction

The rationale of the research is driven by the severity of dry eye syndrome (DES) in the pterygium recurrencies development as well as by the necessity to investigate tear dysfunction and methods for its optimal correction in this patient population. Purpose of the study. To assess the impact of tear dysfunction indices on the development of recurrent pterygium. Materials and methods. We observed 60 patients (67 eyes) with recurrent pterygium. Patients were divided into four observation groups depending on the number of recurrencies. In order to study the dynamics of the DES manifestations during the postoperative period, pathogenetic therapy was used, which included a tear fluid substitute. All patients underwent a comprehensive assessment of subjective and objective DES indices before and after surgery. Results. A positive dynamics of subjective manifestations and objective indices of DES under the action of a tear substitute after surgery was reliably confirmed. A decrease in the number of patients with type III and IV crystallization after surgery was confirmed. Conclusion. The obtained data indicate an increase in the mucin content in the tear fluid composition, which leads to a stabilization of the tear film and to a decrease in the DES intensity.

scholarly journals High-level of circulating progranulin and its relationship with plasma glycosaminoglycans, as biochemical indicators of proteolytic and oxidative aggrecan modification, in the course of juvenile idiopathic arthritis

2018 ◽  
Vol 72 ◽  
pp. 906-912
Author(s):  
Katarzyna Winsz-Szczotka ◽  
Kornelia Kuźnik-Trocha ◽  
Katarzyna Komosińska-Vassev ◽  
Wojciech Lemski ◽  
Krystyna Olczyk
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Healthy Children ◽  
Biochemical Indicators ◽  
Antioxidative Status ◽  
Matrix Metalloproteinase 3 ◽  
Reactive Protein ◽  
Before And After ◽  
Total Antioxidative Status ◽  
A Disintegrin And Metalloproteinase ◽  
High Level

Aim: Progranulin (PGRN) plays an important role in cartilage metabolism. The disturbed interaction between PGRN and glycosaminoglycans (GAGs), as biochemical indicators of aggrecan modification, may contribute to articular damage observed in the course of juvenile idiopathic arthritis (JIA). Hence, the aim of this study was to assess quantitatively the level of progranulin in children with JIA as well as to evaluate the correlation between PGRN and GAGs, MMP-3 (matrix metalloproteinase 3), ADAMTS-4 (a disintegrin and metalloproteinase with thrombospondin motifs 4) as well as the total oxidative status (TOS) and the total antioxidative status (TAS). We have also evaluated interactions between PGRN and inflammatory and anemia indicators, i.e. C-reactive protein (CRP), and hemoglobin (Hb), respectively. Material/Methods: The PRGN level was measured using the immunoenzymatic method, in blind tested coded plasma samples, obtained from both JIA patients before and after treatment and from healthy children. Results: Increased (p<0.001) plasma progranulin in JIA patients before treatment was observed. Therapy resulted in a decrease in the PRGN level. However, the plasma PRGN level still remained higher (p<0.05) than in the controls. In both groups of patients, we have revealed an insignificant correlation between plasma PGRN and GAGs levels. Moreover, a significant correlation between plasma PGRN level and MMP-3, ADAMTS-4, TOS, TAS, CRP and Hb levels, was stated in untreated JIA patients. Conclusions: The obtained results may indicate that PGRN has antioxidative properties in the course of JIA, but do not confirm the protective roles of this glycoprotein in respect to the destructive effects of proteolytic factors.

scholarly journals Management of Achatina fulica (Bowdich, 1822) (Pulmonata: Achatinidae) in lettuce (Lactuca sativa L.)

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Lindinalva Santos ◽  
Carla Barbosa Negrisoli ◽  
Maciel Bispo Santos ◽  
Aldomario Negrisoli Junior
Keyword(s):  
Enzymatic Activity ◽  
Lipase Activity ◽  
Protein Concentration ◽  
Lethal Dose ◽  
Alcoholic Extract ◽  
Achatina Fulica ◽  
Before And After ◽  
Important Pest ◽  
Lethal Time

ABSTRACT: The giant African snail Achatina fulica was introduced in Brazil and since then has become an important pest, because of its resistance to abiotic conditions, hermaphroditism, polyphagia, and absence of natural predators. This study aims to evaluate the control of A. fulica in lettuce, in Alagoas, Brazil. Bioassays for the determination of lethal dose and lethal time to adults of A. fulica and the egg mortality were performed in the laboratory by applying commercial synthetic products, commercial and non-commercial alcoholic botanical extracts on mollusk adults. Additionally, the protein concentration, lipase activity and enzyme acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE), in the stomach, intestine, nervous ganglion and liver were determined. The alcoholic extract of Capsicum frutescens caused higher mortality of A. fulica, and the alcoholic extract of C. frutescens and Piper tuberculatum oil can prevent the hatching of A. fulica. The lipase activity was present and in greater quantities in tissues, stomach, intestine, liver and ganglia of A. fulica, before and after exposure of the alcoholic extract of C. frutescens. The enzymatic activity of BuChE was present in the ganglia and liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The enzymatic activity of AChE was present only in the ganglion and absent in liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The concentration of 10% of the alcoholic extract of C. frutescens caused 84% mortality of adult A. fulica in lettuce in field conditions.

Myocardial enzymatic activity of renin and cathepsin D before and after bilateral nephrectomy

2001 ◽  
Vol 96 (6) ◽  
pp. 659-668 ◽  
Author(s):  
Stephen A. Katz ◽  
John A. Opsahl ◽  
Lynn M. Forbis
Keyword(s):  
Enzymatic Activity ◽  
Cathepsin D ◽  
Bilateral Nephrectomy ◽  
Before And After

scholarly journals PROTEOMIC DIAGNOSTIC TECHNOLOGIES AND THEIR APPLICATION FOR THE REFRACTIVE CORRECTION OF AMETROPIA

2017 ◽  
Vol 12 (4) ◽  
pp. 193-199
Author(s):  
M. A Kovalevskaya ◽  
I. V Chernikova ◽  
V. S Podoprigora ◽  
Mariya I. Sergeeva
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Dry Eye Syndrome ◽  
Tear Fluid ◽  
Antioxidant Protection ◽  
Before And After ◽  
Group 3 ◽  
Group 2 ◽  
High Degree ◽  
Group 1

Aim. The objective of the present study was the analysis of the tear fluid proteomic status and the eye surface of the patients presenting with ametropia before and after laser-assisted in situ keratomileusis (LASIK). Material and methods. The study included 163 patients (326 eyes) divided into three groups. Group 1 was comprised of 51 adult patients (102 eyes)at the age from 35 to 54 years suffering from high-degree myopia and astigmatism. Group 2 was composed of 26 adult patients (52 eyes) at the age from 35 to 55 years suffering from moderate or high-degree hyperopia and astigmatism. Group 3 consisted of 86 younger patients (172 eyes) at the age from 18 to 54 years suffering from high-degree myopia and astigmatism. The dry eye syndrome and the state pf the cornea were diagnosed with the use of the Lipkof’s test, Schirmer’s test, TBUT test, and corneal fluorescence staining. The antioxidant activity of the tears was estimated based on the results of measurements of peroxyredoxin-6 (PEDX6) expression. Results. The level of PEDX6 expression after LASIK increased in the patients presenting with myopia by 1.4 times and in those with hyperopia by 1.07 times, that is it changed but slightly after the operation and was 6.2 times less pronounced in comparison was the myopes. Conclusion. The study has demonstrated that two methods proved reliable for the evaluation of the tear fluid proteomic status, viz. the Lipkof’s test (1.82 ± 0.2mm, stage 3) and the TBUT test (8.0 ± 1.46 seconds), suggesting the presence of grade 2 dry eye syndrome. Te same refers to the patients of group 2: the results of the Lipkof’s test (1.31 ± 0.2mm) and the TBUT test (8.23 ± 0.87 seconds). The level of antioxidant protection of the ocular surface (PEDX6 expression) depended on the type of the refractive error: in the patients of group 1 before and after LASIK, PEDX6 expression was 4.7 times (13.57 ± 0.83) and 6.2 (19.31 ± 0.71) higher 2.88 ± 0.38 and 3.09 ± 0.47) than in those of group 2, respectively. In addition, it was shown that the level of antioxidant protection of the ocular surface (PEDX6 expression) depended on the age of the patients prior to LASIK: it was 1.3 lower in group 1 than in group 3 (1.35 ± 0.83 and 17.77 ± 1.03 respectively). The clinical significance of the tests for the tear fluid production and proteomic status changed appreciably after LASIK. The TBUT test for diagnostics of dry eye syndrome remained reliable in all the groups.

Renin in the Mouse Kidney Has a Molecular Weight of 40 000

1981 ◽  
Vol 60 (1) ◽  
pp. 41-46 ◽  
Author(s):  
K. Poulsen ◽  
A. H. Nielsen
Keyword(s):  
Molecular Weight ◽  
Enzymatic Activity ◽  
High Molecular Weight ◽  
Enzyme Inhibitors ◽  
Polyacrylamide Gel Electrophoresis ◽  
Limited Proteolysis ◽  
Gel Filtration ◽  
Mouse Kidney ◽  
Antigenic Properties ◽  
Before And After

1. Mouse kidney was homogenized in a mixture of serine-metallo- and thiol-enzyme inhibitors. The homogenate proteins were separated with respect to size and charge by gel filtration, agarose electrophoresis and polyacrylamide-gel electrophoresis. 2. Renin was localized by its enzymatic activity by using the antibody trapping radioimmunoassay for angiotensin I, before and after acid treatment and limited proteolysis. Renin was also localized by its antigenic properties by using antirenin antibodies elicited against pure mouse submaxillary renin. The antibody cross-reacted fully with mouse kidney renin and with high-molecular-weight renin forms in mouse plasma. 3. In the kidney only fully enzymically active 40 000 renin could be detected enzymically and antigenically. No high-molecular-weight renin or inactive renin was demonstrable. 4. Two electrophoretically different renin forms were seen in accordance with renin being a glycoprotein. They were both fully enzymically active with identical specific enzymatic activities. 5. The mouse kidney renin had a specific enzymatic activity identical with that of pure mouse submaxillary renin, being 0.4 Goldblatt unit/μg.

scholarly journals Antioxidative status of saliva before and after non-surgical periodontal treatment

2013 ◽  
Vol 141 (3-4) ◽  
pp. 163-168 ◽  
Author(s):  
Nada Novakovic ◽  
Sasa Cakic ◽  
Tatjana Todorovic ◽  
Biljana Andjelski-Raicevic ◽  
Ivan Dozic ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Colorimetric Method ◽  
Periodontal Treatment ◽  
Sod Activity ◽  
Clinical Attachment Loss ◽  
Bleeding On Probing ◽  
Antioxidative Status ◽  
Before And After ◽  
Commercial Kits ◽  
Non Surgical Treatment

Introduction. Oxidative stress and antioxidants play an important role in the pathogenesis of inflammatory disease, including chronic periodontitis (CP). Saliva contains enzymatic (glutathione peroxidase - GPx, superoxide dismutase - SOD, etc.) and non-enzymatic (albumin - ALB, uric acid - UA, glutathione, etc.) antioxidants. Objective. The aims of this study were to investigate: a) level of SOD, GPx, UA, ALB and total antioxidative status (TAS) of saliva in CP patients before and after non-surgical treatment, and b) correlations between clinical periodontal parameters and levels of salivary antioxidants. Methods. Saliva was collected from 21 CP patients before and after non-surgical treatment. The condition of periodontium was assessed by plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss. Level of investigated antioxidants (except GPx) and TAS was determined using colorimetric method and commercial kits. GPx activity was determined using UV method and commercial kits. Results. After the treatment significant increase of UA, ALB, Gpx, TAS was detected (p<0.01) and decrease of SOD activity (p>0.05). A significant correlation was observed between GPx and PI (r=0.575, p=0.008), SOD and GI (r=0.525, p=0.017) before therapy, and SOD and bleeding on probing (BP) (r=0.59, p=0.006), TAS and BP (r=0.453, p=0.045) after therapy. Conclusion. These data suggest that levels of salivary antioxidants generally increase after non-surgical periodontal treatment. Correlation between some clinical periodontal parameters and level of salivary antioxidants was found.

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