scholarly journals Antibiotic resistant and phagosensitivity of regional strains-causative agents of pyo-inflammatory diseases (S. aureus and P. aeruginosa).

2020 ◽  
Vol 24 (1) ◽  
pp. 6-11
Author(s):  
S.А. Dеrкаch ◽  
N.I. Gorodnicka ◽  
N.M. Kutsaj ◽  
L.S. Gabisheva ◽  
О.V. Коtsar
Keyword(s):  
Inflammatory Diseases ◽  
Antibiotic Sensitivity ◽  
Bacteriological Examination ◽  
Commercial Preparation ◽  
Antibiotic Resistant ◽  
Causative Agents ◽  
Student’S T ◽  
Antibiotics Sensitivity ◽  
Student’S T Test

Annotation. The actual issue is studying of sensitivity to antibiotics of regional strains-causative agents when there is increasing circulation of poly-resistant microorganisms. They were isolated from different biotopes. Their sensitivity to various commercial bacteriophage preparations was carried out in parallel. The purpose of the study was to determine the antibiotics of choice for starting therapy of purulent-inflammatory diseases and the prospects of using bacteriophage preparations relative to the most etiologically significant regional pathogen strains. Common methods of investigation were used: disco-diffusion to determine antibiotics sensitivity and drop method to study phagosensitivity. The results were processed by the method of variational statistics using M. Excel 2000 Biostat with Student's t test. It has been shown that antibiotics of choice for starting therapy of purulent-inflammatory diseases, in the absence of bacteriological examination of patients and determination of antibiotic sensitivity of pathogens, it is advisable to consider fluoroquinolones (sensitivity of S. aureus and P. aeruginosa to ciprofloxacin, lomefloxacin, norfloxacin about 90%), amikacin and gentamycin (78,3–39,7%), imipenem (57,4%). The conducted studies confirm the prospect of using existing bacteriophage drugs, including “Pyofag” and “Intestyfag”, LLC “Farmaxgroup” (Ukraine). The sensitivity of regional strains was 50,0–60,0%. It remains promising to study the effectiveness of the complex using of antibiotics and bacteriophages and to improve the action of commercial preparation of bacteriophages.

scholarly journals The nasal index in brazilian human skeletons

2018 ◽  
Vol 17 ◽  
pp. e18337 ◽  
Author(s):  
Larissa Chaves Cardoso Fernandes ◽  
Marcus Vitor Diniz de Carvalho ◽  
Eduardo Daruge Júnior ◽  
Luiz Francesquini Júnior ◽  
Patrícia Moreira Rabello ◽  
...  
Keyword(s):  
Mixed Race ◽  
Cross Sectional Study ◽  
Brazilian Population ◽  
Cross Sectional ◽  
Significance Level ◽  
Human Skulls ◽  
Student’S T ◽  
Digital Caliper ◽  
Student’S T Test

Aim: To investigate the percentage of correctness (PC) of the Nasal Index (NI) in human skeletons for determination of sex, ancestry and estimation of age in the Brazilian population. Methods: This was a cross-sectional study of 173 human skulls belonging to the Piracicaba Dental School. 93 skeletons were males and 80 females; 34 were aged up to 39 years, 56 between 40 and 59 years, and 83 60 years or older; 96 were from white individuals, 49 were from mixed-race and 28 from black individuals. High-precision digital caliper was used to measure nasal height (NH=ANS-nasion) and the maximum nasal width (NW) values, which were applied into the formula NI=NW/NHx100. The data were submitted to discriminant analysis and Student’s t test with equal variances, Mann-Whitney, F (ANOVA), Tukey and Kruskal Wallis, 5% significance level. Results: Dominant nasal type in the Brazilian population was the mesorine. Males showed nasal height and width values greater than those of females, with statistically significant differences in all measurements (p≤0.021) and PC of 76.6%. Similar nasal measurements were found regardless of age (p>0.05), with PC of 41.7%. Ancestral analysis revealed that black individuals have greater nasal width (26.35) and nasal index (53.67) than white ones (24.60 and 49.25), while mixed-race individuals showed intermediate values (25.36 and 52.13) (p<0.05). Nevertheless, these measurements presented an estimated PC of 54.3%. Conclusion: The Nasal Index can be better used for sex determination than for estimation of age and ancestry in the Brazilian population, as the latter showed intermediate and low percentage of correctness, respectively.

scholarly journals SEQUENTIAL MULTI-ELEMENT DETERMINATION OF TRACE-METALS IN BIODIESEL SAMPLES USING HR-CS FAAS

Química Nova ◽  
2020 ◽  
Author(s):  
Alexandre Jesus ◽  
Ariane Zmozinskia ◽  
Diane Laroque ◽  
Márcia Silva
Keyword(s):  
Absorption Spectrometry ◽  
Element Determination ◽  
Continuum Source ◽  
Flame Atomic Absorption ◽  
Student’S T ◽  
Different Sources ◽  
Certified Values ◽  
Good Agreement ◽  
Student’S T Test

In this work the feasibility of sequential multi-element determination of Zn, Fe, Mg, Cu, Na, K, Cr, Al and Ca in biodiesel samples by high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS) was investigated. Biodiesel samples obtained from different sources and different chemical processes were analysed. The samples were diluted with n-propanol and water (1.4 mol L-1 HNO3) to form a microemulsion (ME) before its introduction in the HR-CS FAAS using a flow injection mode. The sample B-01 (obtained from soybean oil) was used for optimisation of the instrumental parameters. The accuracy of the proposed method was checked by analysis of certified reference material (CRM) Conostan BDM2A (for K and Na) and BDM2B (for Ca and Mg) and by comparison with acid digestion and recovery tests (for Zn, Fe, Cu, Cr and Al). The obtained results for CRM analysis showed good agreement with certified values within 95% of confidence (Student’s t-test). Recovery tests values ranged from 87-114%. The method developed for the analysis of biodiesel samples is accurate, simple, fast, and suitable for routine application.

CuSO4-TiO2 as Kjeldahl Digestion Catalyst in Manual Determination of Crude Protein in Animal Feeds

1986 ◽  
Vol 69 (4) ◽  
pp. 664-666
Author(s):  
Peter F Kane
Keyword(s):  
Standard Deviation ◽  
Crude Protein ◽  
T Test ◽  
Environmental Concerns ◽  
Animal Feeds ◽  
Significant Difference ◽  
Kjeldahl Digestion ◽  
Student’S T ◽  
Student’S T Test

Abstract The official AOAC manual Kjeldahl methods for determining crude protein in animal feeds have several disadvantages. For the HgO catalyst method, there are environmental concerns and a lengthy digestion. For the CuS04 catalyst method, the digestion period is shorter, but still 90 min. A different catalyst combination, CuS04-Ti02, makes 40 min digestion feasible. Comparison of these catalysts on a group of representative feeds resulted in a mean difference, Cu-Ti minus HgO, of 0.034% protein. Standard deviation of the differences was 0.36. A Student’s t-test showed no significant difference. The method will be collaboratively studied.

scholarly journals Development of an Ultraviolet Spectrophotometric Method for the Determination of Ceftiofur Sodium Powder

2009 ◽  
Vol 92 (6) ◽  
pp. 1673-1680 ◽  
Author(s):  
marinês J E Souza ◽  
Natália Canedo ◽  
Paulo S Souza Filho ◽  
Ana M Bergold
Keyword(s):  
Spectrophotometric Method ◽  
Spectrophotometric Analysis ◽  
Uv Spectrophotometry ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Student’S T ◽  
Ceftiofur Sodium ◽  
Interday Precision ◽  
Student’S T Test

Abstract A UV spectrophotometric method was developed for determination of ceftiofur sodium in the drug substance and sterile powder for injection. The method validation, which yielded good results, included evaluation of the range, linearity, intraand interday precision, accuracy, recovery, specificity, robustness, LOQ, and LOD. The UV spectrophotometric determinations were performed at 292 nm. Good linearity was obtained between 2.5 and 20.0 g/mL. A prospective validation showed that the method is linear (r = 0.9999) and precise, with RSD values of 0.3 for product A and 0.4 for product B. The intraand interday precision values were &lt;2 for all samples analyzed. Comparison of UV spectrophotometry and LC by analysis of variance and Student's t-test showed no significant difference between methodologies. Moreover, the accuracy and precision obtained with the UV method correlated well with the values obtained with the LC method, and this correlation suggests that UV spectrophotometric analysis can be an inexpensive, reliable, and less time-consuming alternative to chromatographic analysis. The results demonstrated the validity of the proposed method as a simple and useful alternative for the determination of ceftiofur in routine QC analyses.

scholarly journals Dviejų krūtį tausojančių operacijų – pusmėnulinės segmentektomijos ir naviką adaptuojančios mastopeksijos palyginimas: monocentrinis tyrimas

2012 ◽  
Vol 11 (3-4) ◽  
pp. 72-78
Author(s):  
S. Kravalis ◽  
J. Mayer ◽  
M. Hippach ◽  
B. Reichel-Mayer ◽  
G. Drąsutienė
Keyword(s):  
Surgical Techniques ◽  
Breast Conserving Surgery ◽  
Oncoplastic Surgery ◽  
Chi Square ◽  
Advantages And Disadvantages ◽  
Duration Of Surgery ◽  
Student’S T ◽  
Periareolar Mastopexy ◽  
Student’S T Test

Tyrimo tikslasPalyginti krūtį tausojančias operacijas: periareolinę naviką adaptuojančią mastopeksiją su įprasta pusmėnuline segmentektomija, nustatyti ypatumus, metodų pranašumus ir trūkumus, pasirenkant kurį nors operacijos metodą.Tyrimo metodaiAtlikta retrospektyvioji analizė pacienčių, operuotų „Ammerland“ klinikų Krūties centre Vesterštėdėje (Vokietija) 2008–2009 m. dėl pirminio krūties vėžio, atliekant pusmėnulinę segmentektomiją (n = 136) bei periareolinę naviką adaptuojančią mastopeksiją (n = 131). Abiejų operacijų metodikos buvo nagrinėjamos atsižvelgiant į įvairius kriterijus: naviko ir krūties dydį, kūno masės indeksą, rezektato svorį ir kitus. Duomenys nagrinėti naudojant Stjudento t bei chi kvadrato (χ2) testus, Pearsono koreliaciją ir Kendallo tau-b (r), kai p < 0,05.RezultataiPašalinto audinio svoris (36,96 g ± 20,68 vs. 53,13 g ± 33,03, p = 0,0001, p < 0,05) ir naviko skerspjūvis (17,63 mm ± 11,94 vs. 25,77 mm ± 17,25, p = 0,0001, p < 0,05) buvo didesni pacienčių, kurioms buvo atlikta naviką adaptuojanti mastopeksija. Mažesnes krūtis turinčios moterys buvo dažniau operuojamos naudojant mastopeksijos metodiką, p = 0,008, p < 0,05.Pakartotinių operacijų skaičius (apie 30 %, p = 0,351) ir naviko atstumas iki rezektato kraštų abiejose grupėse nesiskyrė (segmentektomija – 5,52 mm ± 2,59, mastopeksija – 5,88 mm ± 2,87, p = 0,377). Operacijos laikas buvo 1,7 karto ilgesnis mastopeksijos grupėje (113,4 min. ± 29,42 vs. 67,61 min. ± 26, p = 0,001, p < 0,05). Komplikacijų skaičius abiejų operacijų grupėse nesiskyrė, p = 0,34.IšvadaTaikant naviką adaptuojančių periareoliarinės mastopeksijos operacijų metodiką, galima pašalinti didesnius navikus nei įprastos pusmėnulinės segmentektomijos būdu. Mastopeksija taip pat gali būti taikoma moterims, turinčioms mažesnį kūno masės indeksą bei mažesnes krūtis.Reikšminiai žodžiai: krūtį tausojanti operacija (KTO), onkoplastinė chirurgija, naviką adaptuojanti mastopeksija.Comparison of tumor-adapted mastopexy with semilunar segmentectomy – monocentric study ObjectiveTumor-adapted periareolar mastopexy is a breast-conserving oncoplastic surgery method in the breast cancer treatment. The aim of this study was a comparison of breast-conserving surgeries, tumor-adapted periareolar mastopexy vs. the usual semilunar segmentectomy, determination of characteristic properties, advantages and disadvantages when choosing one or another surgery method.MethodsData on patients from 2008–2009 were retrospectively examined. All patients underwent surgery for primary breast carcinoma by semilunar segmentectomy (n = 136) or by pariareolar tumor-adapted mastopexy (n = 131) at the Breast Center of the Ammerland Clinic in Westerstede, Germany. Both surgical techniques were analyzed on various criteria, such as tumor / breast-relation, BMI, resection weight, etc. The data analysis was performed using Student’s t-test or the Chi-square (χ2) test, the correlation by Pearson and Kendall’s Tau-b (r) when p < 0.05.ResultsThe removed tissue weight (36.96 ± 20.68 g vs. 53.13 ± 33.03 g, p = 0.0001, p < 0.05) and the diameter of the tumor (17.63 ± 11.94 mm vs. 25.77 ± 17.25 mm, p = 0.0001, p < 0.05) were larger in the tumor-adapted mastopexy patient group than in the semilunar segmentectomy group. For women with small breasts, mastopexy was used more frequently, p = 0.008, p < 0.05.The number of secondary surgeries (both groups about 30 perc., (p = 0.351) and the distance between the tumor and the resection margin were the same for both surgical procedures (segmentectomy – 5.52 ± 2.59 mm and mastopexy – 5.88 ± 2.87 mm, p = 0.377). The duration of surgery was 1.7 times longer in mastopexy than in the other technique (113.4 ± 29.42 min vs. 67.61 ± 26 min, p = 0.001, p < 0.05). The complication rate was the same in both methods, p = 0.34.ConclusionPeriareolar tumor-adapted mastopexy allows removing larger tumors than the semicircular segmentectomy. Mastopexy can be indicated for women with a lower BMI and smaller breasts.Key words: breast-conserving surgery (BCS), oncoplastic surgery, tumor-adapted mastopexy.

scholarly journals Antibiotic resistance in Acinetobacter baumannii as agent of surgery infection and approaches to its overcoming by means of deca-methoxinum antiseptic

2019 ◽  
Vol 1 (2) ◽  
pp. 18-22
Author(s):  
O A Nazarchuk ◽  
V I Nahaichuk
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Antibiotic Resistant ◽  
Clinical Strains ◽  
Minimal Inhibitory Concentrations ◽  
Hospital Acquired Infections ◽  
Causative Agents ◽  
Resistant Strains ◽  
Burn Disease ◽  
Hospital Acquired

Introduction. Non-fermenting Gram-negative bacilli are known as one of the most frequent causative agents of hospital-acquired infections. Acinetobacter baumannii, as causative agent of infection complications of different localization, has obtained recently high resistance to anti-biotics and has belonged to ESKAPE group of pathogens. Antimicrobials, recommended for the prophylaxis and therapy of hospital-acquired infections, have been failing in their effectiveness and lead to selection of antibiotic resistant strains of A. baumannii. The aim of this research was to substantiate the way of overcoming of resistance in clinical strains of A. baumannii, by means of synergic antimicrobial activity of antibiotics and antiseptic decamethoxinum®. Material and methods. The research was carried out on 190 clinical strains of A. baumannii, isolated from patients with burn disease during the period 2011–2015. The sensitivity of clinical strains of A. baumannii was determined to such antibiotics as ampicillin/sulbactam, cefoperazone, cefoperazone/sulbactam, meropenem, imipenem, amikacin, ciprofloxacin, gatifloxacin and antiseptic decamethoxinum® (DCM; Registration certificate No UA/14444/01/01 since 24.06.2015. Order of the Ministry of Health of Ukraine No 373). The sensitivity of A. baumannii to antibiotics and DCM was determined by means of disk diffusion test and serial dilution (Order of the Ministry of Health of Ukraine No167 since 05.04.2007; EUCAST expert rules).The study of the influence of antiseptic DCM on the sensitivity of acinetobacteria to antibiotics was studied on 35 clinical strains of A. baumannii, drafted from the general number of isolates enrolled in the research. For this, the sensitivity of A. baumannii to antibiotics in the presence of sub-minimal inhibitory concentrations (subMIC) of DCM was identified. The received experimental data were analyzed by “Statistica 6.0”. Results and discussion. The changes of antibiotic sensitivity profile of A. baumannii for five years were shown. It was found that the sensitivity of A. baumannii to majority of antibiotics, selected for study, decreased significantly. But the only ampicillin/sulbactam was found to have vice versa tendency. We found the rising quantity of antibiotic resistant strains of A. baumannii. At the same time, high resistance of acinetobacteria to fluoroquinolones (ciprofloxacin– 96,1%; gatifloxacin– 95,8%) was found in 2015. The in vitro research of combined activity of DCM antiseptic remedy and early mentioned antibiotics against clinical strains of A. baumannii demonstrated the reveal antibiotic effectiveness. As follows, minimal inhibitory concentrations of antibiotics decreased in 1.5–4 times in the mediums which contained subMIC of DCM. Especially this tendency was found in resistant clinical strains. Conclusion. Under selective influence of antibiotics protected by β-lactamase inhibitors, carbapenems, fluoroquinolones aminoglycosides increase the antibiotic resistance in A. baumannii, causative agents of infectious complications in patients with burn disease. The antiseptic remedy decamethoxinum® helps to improve antibiotic sensitivity in resistant A. baumannii.

Analysis of Sulfites in Shrimp Using Rapid Distillation Followed by Redox Titration1

1988 ◽  
Vol 51 (2) ◽  
pp. 137-138 ◽  
Author(s):  
MARIAN V. SIMPSON ◽  
W. STEVEN OTWELL ◽  
MAURICE R. MARSHALL ◽  
JOHN A. CORNELL
Keyword(s):  
Statistical Analysis ◽  
Steam Distillation ◽  
Accurate Determination ◽  
T Test ◽  
Distillation Method ◽  
Student’S T ◽  
Student’S T Test

The use of rapid steam distillation followed by redox iodine titration provides a rapid and accurate determination of total sufite residual in shrimp. Values obtained for sulfite-treated shrimp using the rapid distillation method gave comparable results to those of the officially recognized Monier-Williams method. Values for the rapid distillation method ranged from 6 to 212 ppm while those of the Monier-Williams procedure ranged from 6 to 241 ppm for untreated and treated shrimps, respectively. Statistical analysis using two-sample Student's t-test indicated that there were no significant differences (p&gt;0.05) for residual levels below 100 ppm but the values obtained by the rapid distillation method and the Monier-Williams procedure were significantly different (p&lt;0.05) at concentrations near and above 100 ppm.

Comparison of Automated Method and Improved AOAC Kjeldahl Method for Determination of Protein in Meat and Meat Products

1981 ◽  
Vol 64 (1) ◽  
pp. 29-31 ◽  
Author(s):  
Debra L McGill
Keyword(s):  
Paired Comparison ◽  
Meat Products ◽  
Protein Determination ◽  
Automated Method ◽  
Significant Difference ◽  
Kjeldahl Method ◽  
Student’S T ◽  
Meat Samples ◽  
Student’S T Test

Abstract The Kjel-Foss automated method for protein determination in meat and meat products was compared with the improved AOAC Kjeldahl method. Meat samples were separated into 3 categories based on fat content and analyzed in duplicate by both methods. No significant difference was found in a paired comparison of the 2 methods in each of the 3 meat categories, using Student’s t-test at the 99% confidence level. A number of additional meat samples analyzed 6-9 times by the automated method showed an overall average range of 0.55% protein and average standard deviation of 0.20. The Kjel-Foss automated method was applicable for total protein determination in a wide variety of meat and meat products.

scholarly journals MAIN AGENTS OF OPPORTUNISTIC INFECTIONS OF GENITOURINARY TRACTS AND THEIR ANTIBIOTIC RESISTANCE

2017 ◽  
Vol 3 ◽  
pp. 18-25
Author(s):  
Gleb Bondarenko ◽  
Svetlana Dzhoraeva ◽  
Anna Kondakova ◽  
Iuliia Shcherbakova ◽  
Nataliya Sobol ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Opportunistic Infections ◽  
Inflammatory Diseases ◽  
Antibiotic Sensitivity ◽  
Genitourinary Tract ◽  
Pathogenic Microorganisms ◽  
Infectious Agents ◽  
The One ◽  
Taxonomic Groups

Today the one of most important medical-social problems is a growing antibiotic resistance of opportunistic infectious agents. Aim of research: the dynamic observance of antibiotic resistance of opportunistic pathogens – infectious agents of genitourinary tracts under conditions of venereological clinic. The study included 2205 patients with inflammatory diseases of genitourinary tract, who were on the stationary treatment at the department of venereology of SI “IDV NAMS of Ukraine”. Bacteriological studies for isolation of conventionally pathogenic microorganisms with determination of sensitivity of separated laboratory strains to antibacterial drugs of the different groups were carried out using the methods of classic bacteriology. It was demonstrated, that the composition of microbiocenosis of genitourinary tract in patients with infections, caused by conventionally pathogenic microorganisms was characterized by the prevalence of aerobic representatives of Enterobacteriaceae and Staphylococcaceae families that formed associations both between each other and with representatives of other taxonomic groups. The analysis of antibiotic sensitivity of separated strains testifies to the necessity of permanent microbiological monitoring of opportunistic infectious agents for elaborations of the regimes of rational antibacterial therapy.

EVALUATION OF THE EFFECTIVENESS OF EMPIRICAL ANTIBIOTIC THERAPY IN PATIENS WITH CHRONIC CALCULOUS PYELONEPHRITIS

2020 ◽  
pp. 111-115
Author(s):  
Андрей Владимирович Кузьменко ◽  
Владимир Васильевич Кузьменко ◽  
Тимур Асланбекович Гяургиев ◽  
Иван Иванович Баранников
Keyword(s):  
Urine Culture ◽  
Antibiotic Sensitivity ◽  
Empirical Therapy ◽  
Bacteriological Examination ◽  
Antibacterial Drugs ◽  
Wide Availability ◽  
The Moment ◽  
Almost All ◽  
Near Future

На сегодняшний день проблема возросшей антибиотикорезистентности патогенных микроорганизмов существенно возросла и является одной из самых обсуждаемых в научном сообществе. Это связано с частобесконтрольным назначением антибиотиков врачами, а такжеширокой доступностью практически всех видов существующих антибактериальных препаратов для пациентов, что дает им возможность самостоятельно приобрести и принимать их, без соответствующего контроля специалиста.На настоящий момент на фармацевтическом рынке отсутствуют существенно новые антибактериальные препараты с принципиально новым механизмом действия и появления их в ближайшем будущем не предвидится. В связи с тем, что пациенты, имеющие заболевания вызванные микроорганизмами нуждаются в назначении антибиотиков с первого дня начала болезни, а бактериальный посев мочи с определением чувствительности микроорганизмов к антибактериальным препаратам занимает от 3 до 7 дней, проблема эмпирической терапии становится ещё более актуальной. Постоянно меняющийся спектр антибиотикочувствительности микроорганизмов обуславливает необходимость постоянного его мониторинга и регулярной оценки эффективности проводимой эмпирической терапии. Целью данной работы стало изучение соответствия чувствительности микроорганизмов, выявляемых при бактериологическом исследовании мочи у больных уролитиазом, осложненным пиелонефритом, антибиотикам, назначаемым пациентам в качестве эмпирической терапии To date, the problem of increased antibiotic resistance of pathogens has increased significantly and is one of the most discussed in the scientific community. This is due to the often uncontrolled prescribing of antibiotics by doctors, as well as the wide availability of almost all types of existing antibacterial drugs for patients, which gives them the opportunity to independently purchase and take them, without the appropriate supervision of a specialist. At the moment, there are no significantly new antibacterial drugs with a fundamentally new mechanism of action on the pharmaceutical market, and their appearance is not expected in the near future. Due to the fact that patients with diseases caused by microorganisms need to prescribe antibiotics from the first day of the onset of the disease, and bacterial urine culture with the determination of the sensitivity of microorganisms to antibacterial drugs takes from 3 to 7 days, the problem of empirical therapy becomes even more urgent. The constantly changing spectrum of antibiotic sensitivity of microorganisms requires constant monitoring and regular evaluation of the effectiveness of empirical therapy. The aim of this work was to study the compliance of the sensitivity of microorganisms detected during bacteriological examination of urine in patients with urolithiasis complicated by pyelonephritis with antibiotics prescribed to patients as an empirical therapy

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