scholarly journals A DIFFERENTIATED APPROACH TO INFUSION-TRANSFUSION THERAPY FOR LARGE BLOOD LOSS IN PATIENTS WITH POLYTRAUMA

World Science ◽  
2021 ◽  
Author(s):  
Tyhai Yuliia ◽  
Mykhnevych Kostiantyn
Keyword(s):  
Lipid Peroxidation ◽  
Blood Loss ◽  
Malonic Dialdehyde ◽  
Antioxidant Effect ◽  
Blood Levels ◽  
Energy Status ◽  
Transfusion Therapy ◽  
Oxygen Transport System ◽  
Grade Iii ◽  
Pyruvate Ratio

With polytrauma, more than 30% of victims are diagnosed with severe blood loss, grade III MARINO of the leading components is transfusion therapy, which aims to restore the erythron system and improve the functional state of the oxygen transport system. The addition to intensive care of substances that affect lipid peroxidation and energy status of cells can reduce the number of heterogeneous transfusions and thus reduce the number of complications in the postoperative period. Such substances may be a solution of ceruloplasmin with a leading antioxidant effect and a solution of D-fructose-1,6-diphosphate sodium salt of the hydrate with an energetic effect. Criteria for exposure to these substances should be indicators such as blood levels of hemoglobin, phosphorus, malonic dialdehyde and lactate / pyruvate ratio.

scholarly journals Antioxidant activity of catecholamines as a chane of them antistress effect

2014 ◽  
Vol 12 (2) ◽  
pp. 43-46 ◽  
Author(s):  
Georgii Nolianovich Shilov ◽  
Vladislav Adamovich Ivanyutin
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Brain Cortex ◽  
Malonic Dialdehyde ◽  
Receptor Activation ◽  
Antioxidant Effect ◽  
Water Soluble ◽  
Antioxidant Mechanism ◽  
Antistress Effect ◽  
The Brain

The antioxidant activity of some native catecholamines (adrenaline, noradrenaline, dopamine), water-soluble antioxidant emoxipine, agonist (apomorphine) and antagonist (haloperidol) of dopamine receptors was assessed as their influence on malonic dialdehyde content and products of lipid peroxidation in homogenates and suspension of the rat cortical brain cells. All catecholamines (adrenaline, noradrenaline, dopamine in concentrations of 10-4 M and 10-5 M) possessed a high (compartable with emoxipine) antioxidant activity. The most antioxidant effect was registered in apomorphine. The inhibitory action of apomorphine on lipid peroxidation in the brain cortex can be a result from both dopamine receptor activation and “direct” antioxidant mechanism.

scholarly journals ОСОБЛИВОСТІ РЕАКЦІЇ ГЛУТАТІОНОВОЇ СИСТЕМИ ВНУТРІШНІХ ОРГАНІВ НА ТЛІ ЕКСПЕРИМЕНТАЛЬНОЇ ПОЛІТРАВМИ

2018 ◽  
Author(s):  
N. V. Volotovska ◽  
A. A. Hudyma
Keyword(s):  
Lipid Peroxidation ◽  
Blood Loss ◽  
Ischemia Reperfusion ◽  
Malonic Dialdehyde ◽  
Kidney Tissue ◽  
Male Rats ◽  
Mechanical Trauma ◽  
Control Group ◽  
Glutathione System ◽  
Glutathione Concentration

Nowadays stop the bleeding and fight against its consequences determines further success in the survival and rehabilitation of the victim. It is extremely important to find ways to improve the ischemic metabolism as a result of overlaying a tissue turnstile. Enzymes of the glutathione system are actively involved in combating the effects of hypoxia, therefore, the experimental detection of critical periods of depression of this protectional link is the basis for the further search for effective methods of correction and condition stabilization of the affected organism.The aim of the study – to determine the role of glutathione system of the liver and kidneys in the pathogenesis of experimental ischemia-reperfusion (EIR) in the early and remote post-traumatic periods.Materials and Methods. The object of the study – 196 males of white male rats with a body weight 220–240 g, divided into 4 groups, 10 individuals in each. Animals of the control group were withdrawn from the study without surgical intervention, while in the experimental groups, injuries were simulated: a hemostatic tourniquet on the thigh (J) over two hours; isolated blood loss from the femoral vein (40%) – (K); two-hour ischemia, combined with blood loss (J + K) and two-hour ischemia, combined with blood loss and mechanical trauma (J + K + P). The animals were withdrawn from the experiment at 1, 3, 7 and 14 days after the intervention by total bleeding from the heart.Results and Discussion. According to the activation of the lipid peroxidation, which was represented by the growth of the concentration of malonic dialdehyde, diene and trienic conjugates, an active response from the glutathione system was detected: the activation of glutathioneperoxidase activity, an increase of glutathionereductase activity against the decrease restored glutathione concentration. But in the kidney tissue the activity of glutathione system enzymes was somewhat lower, herewith in some periods, conversly, they exceeded liver parameters. These results, in our opinion, indicate that the reason of such their dividing, mainly, it is total debilitation of the glutathione system in the posttraumatical period. It was 1 and 3 days after trauma and partly depended of the injury severity. At the same time, even an isolated overlay of the hemostatic tourniquet led to significant changes of the glutathione system and was represented by response to the activation of lipid peroxidation. This confirms the necessity to continue the series of experiments.Conclusions. The obtained results of the experiments revealed a significant inhibition of the reduced glutathione concentration in the liver, and no so expressed in the kidneys. Especially depression of its activity was on the background of bleeding associated with ischemia, and bleeding associated with ischemia and mechanical trauma. Decrease of glutathioneperoxidase and glutathionereductase activity coincided with increase of lipid peroxidation.

ECOLOGICAL AND HYGIENIC INVESTIGATION OF THE ADAPTOGENIC HERBAL PRODUCTS TO INCREASE RESISTANCE OF THE ORGANISM TO CONDITIONS OF LOW AND HIGH TEMPERATURES

2016 ◽  
Vol 1 (61) ◽  
pp. 46-51
Author(s):  
Литовченко ◽  
Ekaterina Litovchenko ◽  
Коршунова ◽  
Natalya Korshunova ◽  
Доровских ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
High Temperatures ◽  
Malonic Dialdehyde ◽  
Antioxidant Effect ◽  
The Body ◽  
Diene Conjugates ◽  
Herbal Products ◽  
Experimental Animals ◽  
White Rats ◽  
Methodological Approaches

The purpose of this research was to study the possibility of using of food products from a mixture of Hypericum perforatum (HP) and Rhodiola rosea (RR) for the stimulation of the body´s compensatory responses to the conditions of high and low temperatures. Experiments have been conducted on experimental animals (150 white mongrel rats) in accordance with generally accepted methodological approaches. Antioxidant effects in the organism of white rats have been studied at the initiation of the processes of lipid peroxidation by the introduction of carbon tetrachloride. We were determined the products of lipid peroxidation (diene conjugates, hydroperoxides of lipids, malonic dialdehyde) for the evaluation of antioxidant effect in accordance with generally accepted methodological approaches. Researches of cold and heat adaptation reactions of experimental animals have been conducted by using the model of a long cold and heat action with appropriate climatic chambers. It was established experimentally that in doses of 150-300 mg/kg daily of the studied mixture has a strong antioxidant effect in the conditions of cold and heat stress on warm-blooded organism. The research allows to recommend a mixture of HP and RR as а regulator of adaptive reactions of the organism when exposed to low and high temperatures.

scholarly journals RESEARCH OF THE EFFECT OF A NEW ANTIHYPOXANT AND ANTIOXIDANT AGENT IN EXPERIMENTAL HYPOXIA CAUSED BY ACUTE BLOOD LOSS

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
Zh Khuzhakhmedov ◽  
L Shevchenko ◽  
H Karimov ◽  
T Alimov ◽  
R Rakhmanberdieva
Keyword(s):  
Lipid Peroxidation ◽  
Blood Loss ◽  
Blood Plasma ◽  
Experimental Model ◽  
Hypoxia Inducible Factor ◽  
Blood Substitute ◽  
Antioxidant Effect ◽  
Acute Blood Loss ◽  
Antioxidant Agent ◽  
Peroxidation Processes

Aim. To study the antihypoxic and antioxidant effect of the new blood substitute «Rheoambrasol» in acute blood loss.Material and methods. The experiments were carried out on 60 rats, in which the experimental model of acute blood loss was reproduced. The effectiveness of using the new blood substitute «Rheoambrasol” in acute blood loss was determined in comparison with the drug “Rheopolyglyukin”.Results. The results obtained, it can be seen that blood loss is accompanied by hypoxia, as evidenced by the concentration of hypoxia-inducible factor (HIF-1α), which increased in the second group by 4.1 times. After the infusion of “Rheoambrasol”, the content of hypoxia-inducible factor HIF-1α in blood plasma decreased 2.9 times, compared with the value after blood loss, which was 44.4% lower than after the infusion of the drug “Rheopolyglucin”. After the use of the drug “Rheoambrasol”, the balance of lipid peroxidation activity/ antioxidant system activity (LPO/AOS) in the blood, myocardium and liver was restored. Conclusion. The new drug “Rheoambrasol” has a pronounced antihypoxant effect in acute blood loss, which is manifested in the ability to reduce the content of HIF-1α in the blood of experimental animals.The introduction of “Rheoambrasol” in acute blood loss effectively inhibits lipid peroxidation processes in the blood, myocardium and liver and increases the activity of AOS enzymes, which indicates its antioxidant effect.

Decreased Cysteine and Glutathione Levels: Possible Determinants of Liver Toxicity Risk in Ghanaian Subjects

1994 ◽  
Vol 22 (3) ◽  
pp. 171-176 ◽  
Author(s):  
N-A Ankrah ◽  
T Rikimaru ◽  
F A Ekuban ◽  
M M Addae
Keyword(s):  
Vitamin A ◽  
Liver Toxicity ◽  
Malonic Dialdehyde ◽  
Serum Levels ◽  
Blood Levels ◽  
Liver Inflammation ◽  
Body Tissues ◽  
Group 2 ◽  
Β Carotene ◽  
Group 1

Cysteine, methionine, vitamin A, β-carotene and glutathione (GSH) are known to protect body tissues against oxidative damage and inflammation but their value as protection against liver inflammation in tropical areas has received little attention. Blood levels of these nutrients were measured in Ghanaian volunteers with (Group 2) or without (Group 1) increased lipid peroxidation and signs of liver inflammation, as indicated by blood malonic dialdehyde, serum α1-antitrypsin and triglyceride levels, and the α1-acid glycoprotein: pre-albumin ratio. Serum levels of cysteine and blood glutathione were significantly lower ( P < 0.02) in group 2 than in group 1 volunteers. In contrast, serum levels of methionine, vitamin A and β-carotene were similar in both groups. Deficits in cysteine and glutathione may increase the risk of liver toxicity from oxidants in Ghanaians.

scholarly journals Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

2012 ◽  
Vol 1 (1) ◽  
pp. 18
Author(s):  
Amrit Kaur Bansal ◽  
Ranjna Sundhey Cheema ◽  
Vinod Kumar Gandotra
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Acrosome Reaction ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Sperm Capacitation ◽  
Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

scholarly journals Analysis of the relationship between hemoglobin-oxygen affinity and lipid peroxidation during fever.

1995 ◽  
Vol 42 (1) ◽  
pp. 69-74 ◽  
Author(s):  
M V Borisiuk ◽  
V V Zinchuk
Keyword(s):  
Lipid Peroxidation ◽  
Malonic Dialdehyde ◽  
Oxygen Affinity ◽  
Alpha Tocopherol ◽  
Diene Conjugates ◽  
Tocopherol Concentration ◽  
Hemoglobin Oxygen Affinity ◽  
The Relationship ◽  
Free Radical Lipid Oxidation ◽  
Oxyhemoglobin Dissociation

Endogenous hyperthermia was induced in rabbits by i.v. pyrogenal administration. Hemoglobin-oxygen affinity and parameters of free radical lipid oxidation in plasma and red blood cells were measured. The content of diene conjugates, malonic dialdehyde and Schiff bases were determined at a pyrogenal dose of 4 minimal pyrogenic doses/kg, and iron-initiated chemiluminescence, catalase activity and alpha-tocopherol concentration were determined at 6 minimal pyrogenic doses/kg. A rightward shift of the real oxyhemoglobin dissociation curve and activation of lipid peroxidation were observed. Relationships between the parameters measured were analyzed. Decreased hemoglobin-oxygen affinity is considered to be a possible mechanism of activation of free radicals during fever.

MEDICAL PREVENTION OF POST-TRANSFUSION COMPLICATIONS AFTER TRANSFUSION THERAPY APPLIED IN CASE OF SEVERE OBSTETRIC BLOOD LOSS

2020 ◽  
Vol 4 (2) ◽  
pp. 1048-1056
Author(s):  
F.N. Karpenko ◽  
◽  
A.V. Novik ◽  
E.D. Rasyuk ◽  
V.V. Pasyukov ◽  
...  
Keyword(s):  
Blood Loss ◽  
Emergency Obstetric Care ◽  
Coagulation Factor ◽  
Obstetric Care ◽  
Coagulation Factors ◽  
Blood Components ◽  
Obstetric Hemorrhage ◽  
Transfusion Therapy ◽  
Modern Approach ◽  
Golden Hour

The article presents an analysis of the modern approach to the treatment of acute obstetric hemorrhage. Some features of the preparation of leukodepleted and pathogen-reduced blood components are shown and indications for use in severe obstetric blood loss are determined. It has been shown that the pathogen reduction of blood components leads to a decrease the level of coagulation factors (coagulation factor VIII, fibrinogen in fresh frozen plasma) by 20-30 %, the activity and number of platelets by 15-20 % in platelet concentrate, does not affect the morphological usefulness of platelets. A "package" of blood components for the provision of emergency transfusion therapy for obstetric bleeding has been calculated. The need for a given quantity of blood components was determined – 2.3 "packages" per 1000 births. The proposed "emergency obstetric care packages" and the organization of their centralized delivery to medical healthcare organizations ensure a high degree of readiness of the blood service to comply with the "golden hour" rule for treating acute severe obstetric hemorrhage and minimize post-transfusion reactions and complications when using them. Pathogen-reduced blood components are expensive. Therefore, their use in clinical practice is indicated for the decreed contingents of recipients: for organ and tissue transplantation, in neonatology, oncohematology and for recipients with “multiple transfusions of blood, its components”, in cardiac surgery and obstetric practice.

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