Sodium Nitroprusside-Mediated Modulation of Growth and Antioxidant Defense in the InVitro raised Plantlets of Peanut Genotypes
ABSTRACT As a bioactive signaling molecule, nitric oxide (NO) is involved in multiple plant physiological responses. It regulates diverse biochemical processes in a concentration-dependent manner in plants. Different NO generators viz. sodium nitroprusside (SNP), S-nitroso-N-acetyl penicillinamine (SNAP) and S-nitroso-L-glutathione (GSNO) have been reported, but SNP is the most widely used and effective NO donor. Research was conducted to investigate the in vitro effects of an NO donor, SNP, on biochemical and physiological characteristics such as multiple shoots, chlorophyll content, and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and others in Arachis hypogaea genotypes (M-13 and PBS24030). In vitro impact of SNP on shoot multiplication potential and chlorophyll content increase upto 100 µM SNP alone in peanut cultivars (M-13 and PBS24030). Rhizogenesis was noticed in the presence of SNP alone. Treatment with SNP and 6-Benzyl adenine (BA) was effective in enhancing the antioxidant enzyme activities, total soluble carbohydrates and proteins as compared to SNP alone in for both cultivars. These data indicate that in vitro establishment of peanut cultivars in the presence of SNP alone and in combination with BA will affect various growth promontory physiological and biochemical parameters. A more complete understanding of plant growth regulator (PGR) mediated responses will be instrumental in designing effective strategies for engineering crops for biotic and abiotic stresses.