scholarly journals Progesterone Decreased Cell Infiltration in Airways of Systemic Sclerosis Mice Model

2020 ◽  
pp. 1-4
Author(s):  
Nazanin Mojtabavi ◽  
Fatemeh Vafashoar ◽  
Kazem Mousavizadeh ◽  
Hadi Poormoghim ◽  
Pendar Safari ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Sex Steroids ◽  
Lung Fibrosis ◽  
Direct Action ◽  
Inflammatory Cells ◽  
Differential Count ◽  
Mice Model ◽  
Control Groups ◽  
Infiltrating Cells ◽  
Parenchymal Cells

Systemic sclerosis (SSc) is the fibrotic autoimmune disease with a higher incidence in women. Lung fibrosis is the most common cause of death in SSc patients. Sex steroids have crucial role in the induction of autoimmune diseases. Progesterone impacts autoimmunity by direct action on parenchymal cells or through its immunomodulatory effect. This study aimed to investigate the effect of progesterone on the cellularity of airways in an animal model of systemic sclerosis. 6 groups of mice were considered in this study. Systemic sclerosis (SSc) was induced in female BALB/c mice by subcutaneous injection of bleomycin for 28 days. For evaluating the effect of Progesterone in SSc model, Progesterone was administered subcutaneously parallel with bleomycin for 28 days or one week after the first administration of bleomycin for 21 days. Further, three control groups were included in this study. On day 29, under lethal anesthesia bronchoalveolar lavage (BAL) was collected and evaluated for cellularity. Our results indicate the increment of cells in BAL of SSc (P<0.0001) mice. Administration of Progesterone for 28 days significantly reduced the infiltrating cells in BALs (P<0.01) of SSc mice. The differential count of BALs indicates that Progesterone reduced the number of lymphocytes (P˂0.05) in SSc mice but did not affect the number of macrophages. Therefore, we conclude that progesterone reduced the inflammatory cells in airways by decreasing the number of lymphocytes.

scholarly journals The protective effect of hydrogen sulfide on systemic sclerosis associated skin and lung fibrosis in mice model

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Zhi Wang ◽  
Xiaoya Yin ◽  
Luyan Gao ◽  
Sheng Feng ◽  
Kai Song ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Systemic Sclerosis ◽  
Protective Effect ◽  
Lung Fibrosis ◽  
Mice Model

The involvement of leucine-rich α-2 glycoprotein in the progression of skin and lung fibrosis in bleomycin-induced systemic sclerosis model

2021 ◽  
pp. 1-10
Author(s):  
Hideki Nakajima ◽  
Kimiko Nakajima ◽  
Satoshi Serada ◽  
Minoru Fujimoto ◽  
Tetsuji Naka ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Lung Fibrosis

Glycogen content is affected differently in acute pulmonary and extra-pulmonary lung injury

2009 ◽  
Vol 28 (9) ◽  
pp. 583-590 ◽  
Author(s):  
EL Borges ◽  
MB Pinheiro ◽  
A. Eleto-Silva ◽  
MV Caliari ◽  
MG Rodrigues-Machado
Keyword(s):  
Lung Injury ◽  
Gastrocnemius Muscle ◽  
Glycogen Content ◽  
Inflammatory Cells ◽  
Severe Form ◽  
Control Groups ◽  
Biochemical Aspect ◽  
Extra Pulmonary ◽  
Anthrone Reagent ◽  
Different Tissues

Acute respiratory distress syndrome (ARDS) is the most severe form of acute lung injury (ALI). The aim of the present study was to investigate whether paraquat-induced acute pulmonary and extra-pulmonary lung injury (ALI-P and ALI-EX, respectively), in rats, affects glycogen content in different tissues. This measurement could indicate performance limitations of tissues, a new biochemical aspect of ARDS. ALI-P and ALI-EX were induced by injection into the trachea (0.5 mg/kg) and intraperitoneally (20 mg/kg) 24 hours prior to tissue collection. The control groups (CTRL) received the same volume of saline. Glycogen content (mg/g tissue) from different tissues was measured using the anthrone reagent. Glycogen content in the heart and kidney was higher in the ALI-EX group than the CTRL-EX group. Glycogen content in the gastrocnemius muscle was lower in the ALI-EX group than the CTRL-EX group. However, there were no significant differences in glycogen content in the diaphragm in the ALI-EX and ALI-P groups or in the gastrocnemius, heart and kidney in the ALI-P group when compared to the respective controls. ALI-EX caused a greater thickening of the alveolar walls, more areas of atelectasis and a greater abundance of inflammatory cells in comparison to ALI-P. These results demonstrate that glycogen content in ALI, induced by an herbicide that is highly toxic to humans and animals, is altered in different tissues depending on the location of the injury.

Hormonal Control of Fish Reproductive Behavior: Brain–Gonadal Steroid Interactions

1982 ◽  
Vol 39 (1) ◽  
pp. 36-47 ◽  
Author(s):  
L. S. Demski ◽  
P. J. Hornby
Keyword(s):  
Reproductive Behavior ◽  
Sex Steroids ◽  
Anterior Commissure ◽  
Direct Action ◽  
Hormonal Control ◽  
Sufficient Evidence ◽  
Brain Areas ◽  
Behavioral Systems ◽  
Goldfish Carassius Auratus ◽  
Tentative Model

A number of hormones are known to influence reproductive behavior in teleosts. The best studied in this regard are the sex steroids estradiol and testosterone. Sufficient evidence is available to permit the outlining of possible modes of action of these hormones on mechanisms controlling reproductive responses in fishes. Autoradiographic studies using tritium-labeled steroids have revealed several brain areas with neurons that concentrate testosterone and estradiol in both males and females. An area near the anterior commissure (preoptic region and parts of the area ventralis telencephali) is of primary interest for behavioral systems. Electrophysiological experiments in goldfish (Carassius auratus) have demonstrated that both hormones strongly influence olfactory mechanisms. These effects may be mediated by steroid feedback on brain areas involved in centrifugal control of the olfactory bulbs. The neurological substrates for several behaviors which are thought to be androgen dependent have been studied using classical neurobehavioral methods. These include: spawning and sperm release in goldfish and sunfish (Lepomis sp.) and courtship and nestbuilding in sunfish. As of yet, a direct action of sex steroids on these systems has not been demonstrated; however, they all relate to the steroid-concentrating area mentioned above. Some of the systems are incorporated into a tentative model of the neural–hormonal mechanisms controlling reproductive behavior in male goldfish.Key words: reproductive behavior, fishes, brain, hormones, sex steroids, olfaction

Differences in Texture Analysis Parameters Between Active Alveolitis and Lung Fibrosis in Chest CT of Patients with Systemic Sclerosis

2017 ◽  
Vol 24 (12) ◽  
pp. 1596-1603 ◽  
Author(s):  
Christopher Kloth ◽  
Anya C. Blum ◽  
Wolfgang M. Thaiss ◽  
Heike Preibsch ◽  
Hendrik Ditt ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Texture Analysis ◽  
Lung Fibrosis ◽  
Chest Ct

scholarly journals Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Nawar Bahjet Kamil ◽  
Nada M. Al-Ghaban
Keyword(s):  
Healing Process ◽  
Inflammatory Cells ◽  
Control Group ◽  
Facial Skin ◽  
Histological Findings ◽  
Control Groups ◽  
Mean Values ◽  
Significant Difference ◽  
The Right ◽  
New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

scholarly journals Mitochondrial Dysfunction in EAE Mice Brains and Impact of HIF1-α Induction to Compensate Energy Loss

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Mahboobeh Mosavimehr ◽  
Seyed Alireza Mesbah-Namin
Keyword(s):  
Energy Loss ◽  
Mitochondrial Dysfunction ◽  
Demyelinating Disease ◽  
Specific Activity ◽  
Hypoxia Inducible Factor ◽  
Neuronal Loss ◽  
Negative Control ◽  
Potential Candidate ◽  
Mice Model ◽  
Control Groups

Background: Mitochondrial dysfunction may be involved in the process of degradation and death of gray matter cells of the central nervous system (CNS) in patients with multiple sclerosis (MS). MS is known as a chronic, progressive demyelinating disease of the CNS. Objectives: Experimental autoimmune encephalomyelitis (EAE) mouse model of MS is the best method for extracting data trend for diagnosing this disorder. The aim of this study was to evaluate the specific activity of the Cytochrome oxidase (COX), ATP, and hypoxia-inducible factor 1 alpha (HIF-1α) in brain tissues of the EAE mice model. Methods: Twenty-one female mice (C57BL/6) were used, 9 for inducing the EAE model and 6 for each of both negative and sham control groups. The specific activity of the COX, ATP, and HIF-1α levels were evaluated in the whole brain of all 3 mice groups. Results: According to the findings, specific COX activity and ATP levels were decreased significantly, which could be due to the mitochondrial dysfunction and neuronal loss in MS lesions, whereas HIF-1α levels increased significantly in the EAE mice group, compared to the sham and negative control groups. The significant increase of HIF-1α levels reinforces the hypothesis that the HIF-1α induction may provide prevention of neuronal death by compensating energy loss under hypoxia-like conditions in EAE mice brains. Conclusions: The results of this study suggest that HIF-1α induction may also be a potential target for controlling the progression of MS, or the development of HIF-1α inducing compounds could be a potential candidate for the management of this disease and provide a rationale to conduct further research in this area.

scholarly journals Wang-Bi Capsule Alleviates the Joint Inflammation and Bone Destruction in Mice with Collagen-Induced Arthritis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Hua Cui ◽  
Haiyang Shu ◽  
Dancai Fan ◽  
Xinyu Wang ◽  
Ning Zhao ◽  
...  
Keyword(s):  
Bone Destruction ◽  
Joint Inflammation ◽  
Inflammatory Cells ◽  
Clinical Effects ◽  
Micro Ct ◽  
Mice Model ◽  
Collagen Induced Arthritis ◽  
Pharmacological Mechanism ◽  
Osteoclast Number ◽  
Wang Bi

Wang-Bi Capsule (WB), a traditional Chinese medicine- (TCM-) based herbal formula, is currently used in clinic for the treatment of rheumatoid arthritis (RA) with positive clinical effects. However, its pharmacological mechanism of action in RA is still obscure. Therefore, this study established a collagen-induced arthritis (CIA) mice model to examine the efficacy of WB by using arthritis score, histological analysis, and micro-CT examination. Proinflammatory cytokines expression, osteoclast number, OPG/RANKL system, and NF-κB activation were then detected to further investigate the mechanism of WB in RA treatment. The results indicated that WB could alleviate the erythema and swelling of paws in CIA mice. It also inhibited the infiltration of inflammatory cells and bone destruction and increased bone density in joints of CIA mice. Mechanistic studies showed that WB treatment decreased the production of IL-1β, IL-6, and TNF-α in serum and joints of CIA mice. Moreover, it reduced the osteoclast number, increased OPG level, decreased RANKL level, and inhibited the activation of NF-κB in joints of CIA mice. In conclusion, this study demonstrated that WB could effectively alleviate disease progression of CIA mice by decreasing the IL-1β, IL-6, and TNF-α levels, modulating the OPG/RANKL system, and inhibiting the activation of NF-κB.

scholarly journals Histological analysis of the biocompatibility of three different calcium hydroxide-based root canal sealers

2006 ◽  
Vol 14 (5) ◽  
pp. 376-381 ◽  
Author(s):  
Heloisa Helena Pinho Veloso ◽  
Roberto Alves do Santos ◽  
Túlio Pessoa de Araújo ◽  
Denise Piotto Leonardi ◽  
Flares Baratto Filho
Keyword(s):  
Calcium Hydroxide ◽  
Study Design ◽  
Inflammatory Reaction ◽  
Root Canal ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Inflammatory Cells ◽  
Control Groups ◽  
Tissue Reactions ◽  
Root Canal Sealers

OBJECTIVE: To histologically evaluate the behavior of the subcutaneous tissue of rats after placement of polyethylene tubes filled with calcium hydroxide-based root canal sealers (SealapexTM, ApexitTM e Sealer 26TM). STUDY DESIGN: The study employed 60 rats, which were divided into eight groups, being four experimental groups with 10 rats each, and four control groups with five rats. Analysis was conducted at 48 hours, 7, 21 and 60 days. RESULTS: Histological analysis revealed that all sealers were irritant; however, the intensity varied between each sealer and as a function of time. At the initial periods (48 hours and seven days), all materials demonstrated similar outcomes, with inflammatory reaction from severe to moderate, respectively. At the final periods (21 and 60 days), the tissue reactions to the implants containing SealapexTM displayed an active status yet with regression, compared to the ApexitTM and Sealer 26TM. The control groups exhibited less inflammatory cells than the experimental groups, keeping a reaction status with regression. CONCLUSIONS: The results achieved in this study revealed that the SealapexTM sealer was the most compatible, followed by ApexitTM and Sealer 26TM.

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