scholarly journals Investigation of enteral nutrition products for Mesophilic Aerobic Bacteria and Cronobacter sakazakii contamination

2021 ◽  
Vol 7 (1) ◽  
pp. 39-44
Author(s):  
Burcu İrem Omurtag Korkmaz
Keyword(s):  
Enteral Nutrition ◽  
Health Risk ◽  
Aerobic Bacteria ◽  
Microbiological Analysis ◽  
Cronobacter Sakazakii ◽  
Microbiological Contamination ◽  
Plate Method ◽  
Ph Values ◽  
Hospital Pharmacies ◽  
Contamination Levels

In this study, it is aimed to analyze for the presence of Mesophilic Aerobic Bacteria (MAB), C.sakazakii, and the pH values of the enteral nutrition products, which are categorized under the special medicinal dietary products in the Turkish Food Codex. 20 enteral nutrition products were obtained from various hospital pharmacies in the province of Istanbul, and the spread plate method was carried out on suitable media. According to the results of microbiological analysis, no MAB and C.sakazakii were found in any of the samples (100%; <1 log cfu/mL). The average pH of the products was found to be 6.68 ±0.09. As a result of the research, it has been seen that all the microbiological contamination levels (100%) of enteral nutrition products are within the values suitable for use and do not pose a health risk.

scholarly journals Microbial Safety of Low Water Activity Foods: Study of Simulated and Durban Household Samples

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
O. A. Ijabadeniyi ◽  
Y. Pillay
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Health Risk ◽  
Water Activity ◽  
Elevated Temperatures ◽  
Aerobic Bacteria ◽  
Cronobacter Sakazakii ◽  
Public Health Risk ◽  
Household Level ◽  
High Sucrose

Sixty household low water activity foods were examined and a simulative study was conducted in a high sugar, low aw almond and macadamia butter to determine the survival of Bacillus cereus and Staphylococcus aureus ATCC 25923. Results obtained from 60 low aw samples collected at household level had some significant differences (P≤0,05) within food categories amongst the various tests. Spices had the highest number of aerobic bacteria, aerobic spore-formers, anaerobic spore-formers, and S. aureus. Mean aerobic colony counts for nuts and spices were 2.30 log CFU/g and 4.40 log CFU/g, respectively. Pathogens such as Escherichia coli and Cronobacter sakazakii were present in nuts, whilst Salmonella spp. was present in chocolates. This implies that certain low aw foods may present a public health risk. In the simulative study, temperature and high sucrose concentrations played a significant role in the survival of B. cereus and S. aureus ATCC 25923. B. cereus was found to be more osmotolerant at both reduced and elevated temperatures (18°C and 25°C) in the 12% sucrose sample in both butters, whilst S. aureus ATCC 25923 seemed to grow better in sucrose-free samples at both temperatures in both butters. This implies that certain low aw foods may present a public health risk. Also, B. cereus, being a spore-forming bacterium, can be osmotolerant at both reduced and elevated temperatures.

Domestic Kitchen Microbiological Contamination and Self-Reported Food Hygiene Practices of Older Adult Consumers

2019 ◽  
Vol 82 (8) ◽  
pp. 1326-1335 ◽  
Author(s):  
ELLEN W. EVANS ◽  
ELIZABETH C. REDMOND
Keyword(s):  
Older Adults ◽  
Aerobic Bacteria ◽  
Food Hygiene ◽  
Microbiological Contamination ◽  
Negative Attitudes ◽  
Hygiene Practices ◽  
Inadequate Knowledge ◽  
Cleaning Equipment ◽  
Increased Risk ◽  
Contamination Levels

ABSTRACT Because of increased susceptibility, older adults have an increased risk of foodborne infection, and data suggest elevated incidence; therefore, food hygiene is essential to reduce the risk. Research suggests older adults' inadequate knowledge and negative attitudes toward food hygiene may increase implementation of unsafe food practices. Data on microbiological contamination of domestic kitchens of older adults are lacking. Therefore, this study aimed to determine microbiological contamination of domestic kitchens of older adults. Food contact surfaces and equipment (n = 1,292) in domestic kitchens (n = 100) of older adults (≥60 years) were analyzed to isolate aerobic bacteria, Enterobacteriaceae, Staphylococcus aureus, and Listeria spp.; self-reported hygiene practices were also recorded. Highest contamination levels were determined on in-use cleaning equipment (dish brushes, dishcloths, sponges) with aerobic bacteria &lt;9.3 log CFU per item, Enterobacteriaceae &lt;8.8 log CFU per item, and S. aureus &lt;7.0 log CFU per item. Reported usage length of dish brushes was significantly correlated (P &lt; 0.05) with Enterobacteriaceae contamination. Significant correlations (P &lt; 0.05) were determined between contamination and reported cleaning frequency of refrigerators. Contamination of hand towels in single-occupant households was significantly greater (P &lt; 0.05) than in multioccupant households. The study facilitates novel comparison between reported hygiene practices with microbial contamination, suggesting older adults fail to implement adequate and regular hygiene practices that may increase the possibility of cross-contamination in the domestic kitchen and the associated risk of foodborne illness. Data from this study have determined a need for older adults to improve food hygiene practices in the domestic kitchen.

Effect of frozen storage on some characteristics of kefir samples made from cow’s and goat’s milk

2021 ◽  
pp. 108201322110037
Author(s):  
Ercan Sarica ◽  
Hayri Coşkun
Keyword(s):  
Organic Acids ◽  
Frozen Storage ◽  
Aerobic Bacteria ◽  
Ph Values ◽  
Flavor Components ◽  
Goat’S Milk ◽  
Volatile Flavor ◽  
Goat's Milk ◽  
Lactobacillus Spp ◽  
Oxalic Acids

This study was aimed to determine the changes in kefir samples (CK and GK) made from cow’s and goat’s milk during frozen storage. The CK and GK samples were first stored at +4 °C for 14 and 21 days. Thereafter, all the samples were frozen at –35 °C for 24 h and kept at –18 °C for 45 days. There was no significant change in the fat, protein, acidity and pH values in both samples during the storage. The values of viscosity, WI and C* were higher in the CK samples while the syneresis value was higher in the GK samples throughout the frozen storage. The microorganisms ( Lactococcus spp., Lactobacillus spp., Leuconostoc spp., total mesophilic aerobic bacteria and yeasts) found in kefir made from goat's milk were more affected from the frozen storage. In both samples, the changes in organic acids and volatile flavor components were not significant during frozen storage, except acetic, citric and oxalic acids and acetaldehyde in GK sample. In addition, CK samples were preferred sensorially more by the panellists during frozen storage.

scholarly journals Microbiological Stability of Rice Tart Stored at Ambient Temperature after Baking

2017 ◽  
Vol 6 (5) ◽  
pp. 41
Author(s):  
Nguessan Elise ◽  
Cisse Mohamed ◽  
Niyonzima Eugene ◽  
Sindic Marianne
Keyword(s):  
Staphylococcus Aureus ◽  
Storage Conditions ◽  
Aerobic Bacteria ◽  
Microbiological Analysis ◽  
End User ◽  
European Regulation ◽  
Bacteria Growth ◽  
Safety Standards ◽  
Consumer Safety ◽  
Microbiological Stability

Very popular in Belgium, rice tart is a hot pastry sold in bakeries. It is then consumed at home, for dessert or snack. This study is conducted to investigate the microbiological stability of this foodstuff, from the end of baking to end user by consumers. In this purpose, 108 rice tart samples were collected from each of seven bakeries in five Belgium provinces. Physico-chimical analysis in addition to microbiological analysis were carried out in accordance with the European Regulation EC 2073/2005 and with references methods, to enumerate the total microorganisms count, Staphylococcus aureus, Bacillus cereus as well as Enterobacteria, susceptible likely to contaminate the tarts during the production or after baking. Even when the results meet the microbiological safety standards immediately after baking in all bakeries implicated, a significant (p <0,05) increase of mesophilic aerobic bacteria (ranging from <1 to 7 log cfu/g) and B. cereus (> 3,7 log cfu/g) was observed during the storage at nonrefrigerated temperature (28 to 30°C). A post-baking recontamination and other parameters like an insufficient baking time or a non uniform distribution of the oven heat could explain the observed bacteria growth. The present study shows that most rice tarts investigated are microbiologically safe. However, the possible increase of bacteria load in this foodstuff attributable to the favorable aw, pH and temperature conditions, highlight the importance of applying good hygienic practices and compliance with storage conditions after baking to ensure consumer safety. 

Behavioral Observation and Microbiological Analysis of Older Adult Consumers' Cross-Contamination Practices in a Model Domestic Kitchen

2018 ◽  
Vol 81 (4) ◽  
pp. 569-581 ◽  
Author(s):  
ELLEN W. EVANS ◽  
ELIZABETH C. REDMOND
Keyword(s):  
Older Adults ◽  
Food Safety ◽  
Behavioral Observation ◽  
Microbiological Analysis ◽  
Cross Contamination ◽  
Microbiological Contamination ◽  
Food Handling ◽  
Safety Practices ◽  
Handling Practices ◽  
Food Handling Practices

ABSTRACT The incidence of foodborne illness is higher in older adults because of their increased susceptibility; therefore, food safety practices are important. However, inadequate knowledge and negative attitudes toward food safety have been reported, which may increase use of unsafe food handling practices. Data on the actual food safety behaviors of older adults are lacking. In this study, food safety practices of older adults were observed and linked to microbiological analysis of kitchen surfaces to identify suspected routes of contamination. Older adults (≥60 years, n = 100) prepared a set meal in a model domestic kitchen sanitized according to a validated protocol to ensure minimal and consistent microbiological loads. Food safety behaviors were observed using ceiling-mounted cameras and recorded using a predetermined behavioral checklist. Surface microbiological contamination also was determined after food preparation. Overall, older adults frequently implemented unsafe food handling practices; 90% failed to implement adequate hand decontamination immediately after handling raw chicken. For older adults who used a larger number of adequate hand decontamination attempts, microbiological contamination levels in the kitchen following the food preparation session were significantly lower (P &lt; 0.001). The novel utilization of behavioral observation in conjunction with microbiological analysis facilitated identification of potentially unsafe food handling practices as suspected routes of microbiological cross-contamination in a model domestic kitchen. Findings indicate the potential impact on domestic food safety of unsafe food handling practices used by older adult consumers. This innovative approach revealed that a large proportion of older adults implement behaviors resulting in microbiological cross-contamination that may increase the risk of foodborne illness in the home.

scholarly journals Microbiological Analysis of Dried Goose Carcasses

2016 ◽  
Vol 39 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Ufuk Kamber ◽  
Hilmi Yaman
Keyword(s):  
Public Health ◽  
Health Risk ◽  
Clostridium Perfringens ◽  
Health Risks ◽  
Viable Count ◽  
Microbiological Analysis ◽  
Potential Health ◽  
Potential Health Risk ◽  
Public Health Risks ◽  
Meat Samples

AbstractMicrobiological studies on chicken meat and carcasses are well documented, but very few studies exist on goose meat and carcasses. Therefore, in this study, dried goose carcass samples were collected from the local households in Kars/Turkey and microbiologically analyzed in terms of public health risks. The total mesophilic viable count was found to be 6.58 (mean log10 CFU g−1) (100%). The number of Enterobacteriaceae was 4.85 (92.8%). Coliform bacteria was counted at the numbers of 2.98 (67.8%), while it was 3.95 (91.1%) for the enterococci, 0.42 (26.7%) for the clostridia, 0.04 (3.5%) for the Clostridium perfringens, and 0.41 (12.5%) for the coagulase positive staphylococci. The numbers of mould and yeast were 0.93 (25%) and 4.81 (94.6%), respectively. Salmonellae and Bacillus cereus could not be isolated in the samples. The results indicate that the dried goose meat samples had poor hygienic quality, contained some of the pathogen microorganisms that are likely to pose a potential health risk.

P.7 Microbiological contamination of enteral nutrition in critically ill patients

1996 ◽  
Vol 15 ◽  
pp. 24
Author(s):  
E. Ferrari Baliviera ◽  
M. Maresca ◽  
V. Vitalone ◽  
L. Spagnesi
Keyword(s):  
Enteral Nutrition ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Microbiological Contamination

MICROBIOLOGICAL ANALYSIS OF TILAPIA AND WATER IN AQUACULTURE FARMS FROM SINALOA

Biotecnia ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 20-26
Author(s):  
José Antonio Valenzuela-Armenta ◽  
Sylvia Páz Díaz-Camacho ◽  
Julio Adalberto Cabanillas-Ramos ◽  
Magdalena de Jesus Uribe-Beltrán ◽  
Mária del Carmen De la Cruz-Otero ◽  
...  
Keyword(s):  
Water Samples ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Aerobic Bacteria ◽  
Microbiological Analysis ◽  
Enterobacter Agglomerans ◽  
E Coli ◽  
The World ◽  
Fishery Products

Tilapia is the most cultivated fish around the world. The fish’s environment can harbor bacteria, especially coliforms, which are not normal biota of fish. These microorganisms are contamination indicator groups (IGs) reflecting the possible presence of pathogens, which can compromise the safety of fishery products. The IGs prevalence was estimated in tilapia and water from 29 aquaculture farms. Total and fecal coliforms (TC, FC), mesophilic aerobic bacteria (MAB), S. aureus, E. coli, Salmonella sp., and Streptococcus sp., were investigated. In tilapia, TC and FC were detected in 64% and 48% of the samples, ranging from 4.0 x 101 to 9.8 x 105, and from 1.0 x 101 to 6.4 x 103 CFU/g, respectively; MAB were detected in 90% of the samples. In water, TC and FC were detected in 57% and 35% of the samples, oscillating from 1.0 x 101 to 2.28 x104, and from 1.0 x 101 to 1.2 x 103 CFU/mL, respectively. MAB were detected in 89% of the water samples. Mostly E. coli and Enterobacter agglomerans were detected. Generally, IGs concentrations meet the Mexican regulations; however, the tilapia’s microbiological quality must be continuously monitored.

Effect of pH and Water Activity on the Growth Limits of Listeria monocytogenes in a Cheese Matrix at Two Contamination Levels

2011 ◽  
Vol 74 (11) ◽  
pp. 1805-1813 ◽  
Author(s):  
M. S. SCHVARTZMAN ◽  
C. BELESSI ◽  
F. BUTLER ◽  
P. N. SKANDAMIS ◽  
K. N. JORDAN
Keyword(s):  
Listeria Monocytogenes ◽  
Water Activity ◽  
Pulsed Field Gel Electrophoresis ◽  
Scale Model ◽  
Ph Values ◽  
Initial Ph ◽  
Growth Initiation ◽  
Lower Effect ◽  
Contamination Levels ◽  
Growth Limits

Listeria monocytogenes can proliferate at the beginning of cheesemaking as the conditions favor growth. The objective of this study was to establish the growth limits of L. monocytogenes in a cheese matrix, in case of potential contamination of the milk prior to cheese manufacture. A semisoft laboratory scale model cheese system was made at different initial pH and water activity (aw) levels with a mix of two strains of L. monocytogenes. A factorial design of five pH values (5.6 to 6.5), four aw values (0.938 to 0.96), and two L. monocytogenes inoculation levels (1 to 20 CFU/ml and 500 to 1,000 CFU/ml) was carried out. Each combination was evaluated in six independent replicates. In order to determine if there was a dominant strain, isolated colonies from the cheeses were analyzed by pulsed-field gel electrophoresis. The data relating to growth initiation were fitted to a logistic regression model. The aw of milk influenced the probability of growth initiation of L. monocytogenes at both low and high contamination levels. The pH, at the concentrations tested, had a lower effect on the probability of growth initiation. At pH 6.5 and aw of 0.99 for low contamination levels and pH 6.5 and aw of 0.97 for high contamination levels, increases in population of up to 4 and 2 log were observed at low and high contamination levels, respectively. This shows that if conditions are favorable for growth initiation at the early stages of the cheesemaking process, contamination of milk, even with low numbers, could lead to L. monocytogenes populations that exceed the European Union's microbiological limit of 100 CFU/g of cheese.

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