scholarly journals PCR based detection and evaluation of fermenting capability of local strains of Saccharomyces cerevisiae

2019 ◽  
Vol 2 (2) ◽  
pp. 23-26
Author(s):  
GUL GHUTAI ABDULLAH ◽  
Naseer Ahmed ◽  
Arsalan Ahmed Shah ◽  
Abdul samad ◽  
Tauseef M Asmat
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reference Strain ◽  
Raw Materials ◽  
Molecular Techniques ◽  
Alcoholic Beverages ◽  
Fruit Samples ◽  
Reaction Test ◽  
Close Relationship ◽  
Long Time ◽  
Growth Shape

On Earth there are several organisms that share close relationship with humankind and Saccharomyces cerevisiae is one of them. Saccharomyces cerevisiae is also known as the brewer´s or baker´s yeast and has been used for fermentation and production of bread and alcoholic beverages since long time. The purpose of this study was to evaluate the fermentation capability of Saccharomyces cerevisiae local isolates. For this purpose 100 fruit samples (mango, sugarcane, orange, honey melon, grapes) were collected from local fruit markets and subjected to identification by standard microbiology tests and advance molecular techniques like Polymerase reaction test (PCR). The results revealed the typical growth, shape and color of S. cerevisiae on YPD media. The growth culture subjected to microscopy showed the budding characteristic of S. cerevisiae.  The initial identified strains by virtue of YPD selective media and microscopy were subjected to PCR. The PCR results revealed the presence of S. cerevisiae in 28 samples among all analyzed samples. Importantly, the presence of S. cerevisiae was detected 30% in oranges, in mango 27.77%, in sugarcane 35% while in honey melon and grapes it was 13.63%, 31.81% respectively. This study concludes that S. cerevisiae was more abundant in sugarcane and grapes analyzed samples. The collected strains of present study may be further used in future studies like ethanol production from raw materials and also used as reference strain when working with samples of unknown nature

scholarly journals Ogo: A Cultural System Moves and Damage of the Environment

2020 ◽  
Vol 1 (3) ◽  
pp. 183-191
Author(s):  
Zainur Wula ◽  
Hadjrah Arifin
Keyword(s):  
Natural Environment ◽  
Shifting Cultivation ◽  
Raw Materials ◽  
Water Infiltration ◽  
Environmental Damage ◽  
Residential Areas ◽  
Cultural System ◽  
Study Approach ◽  
Close Relationship ◽  
Long Time

Humans and the natural environment have a very close relationship; it can even be called interdependence. The relationship is very active and interactive because indeed humans have a very high dependence on fulfilling the necessities of life, the most important of which are clothing, food and shelter which have the main source of raw materials from the natural environment, especially in communities whose farmers depend on the natural environment and land. Therefore, humans have an important role in preserving natural resources and the environment so that the community's life will last for a long time. The culture of people's lives in the shifting cultivation system called ogo is one of the main factors in forest and environmental damage in a broad sense because forests are not only related to grass and timber trees but also land, rocks, water, fauna. The research method used is qualitative with a case study approach. The data were collected through documents, in-depth interviews and observations, and data analysis was carried out descriptively. The results showed that forest and environmental damage due to excessive use with the ogo culture of shifting cultivation with a period of three to four years resulted in reduced water reserves due to damage to water infiltration, floods and landslides as well as damage to residential areas. Roads and bridges in the village of Nuanaga in February 2016. Ogo as a socio-cultural system of shifting cultivation is an act of rational choice by farmers in increasing income and the dignity of family life, despite frequent floods and landslides in the rainy season with high intensity.

scholarly journals GAT1 Gene, the GATA Transcription Activator, Regulates the Production of Higher Alcohol during Wheat Beer Fermentation by Saccharomyces cerevisiae

2021 ◽  
Vol 8 (5) ◽  
pp. 61
Author(s):  
Ya-Ping Wang ◽  
Lin Liu ◽  
Xue-Shan Wang ◽  
Kun-Qiang Hong ◽  
Li-Hua Zhang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Target Genes ◽  
Raw Materials ◽  
Amino Nitrogen ◽  
Alcoholic Beverages ◽  
Higher Alcohols ◽  
Transcription Activator ◽  
Higher Alcohol ◽  
Alcohol Production ◽  
Double Copy

Uncoordinated carbon-nitrogen ratio in raw materials will lead to excessive contents of higher alcohols in alcoholic beverages. The effect of GAT1 gene, the GATA transcription activator, on higher alcohol biosynthesis was investigated to clarify the mechanism of Saccharomyces cerevisiae regulating higher alcohol metabolism under high concentrations of free amino nitrogen (FAN). The availability of FAN by strain SDT1K with a GAT1 double-copy deletion was 28.31% lower than that of parent strain S17, and the yield of higher alcohols was 33.91% lower. The transcript levels of the downstream target genes of GAT1 and higher alcohol production in the double-copy deletion mutant suggested that a part of the effect of GAT1 deletion on higher alcohol production was the downregulation of GAP1, ARO9, and ARO10. This study shows that GATA factors can effectively regulate the metabolism of higher alcohols in S. cerevisiae and provides valuable insights into higher alcohol biosynthesis, showing great significance for the wheat beer industry.

scholarly journals Impact of Fear of Contracting COVID-19 and Complying with the Rules of Isolation on Nutritional Behaviors of Polish Adults

2021 ◽  
Vol 18 (4) ◽  
pp. 1631 ◽  
Author(s):  
Iwona Kowalczuk ◽  
Jerzy Gębski
Keyword(s):  
Eating Behaviors ◽  
Eating Habits ◽  
Food Product ◽  
Alcoholic Beverages ◽  
Online Study ◽  
Food Purchases ◽  
Product Consumption ◽  
Close Relationship ◽  
Two Factors

The aim of the study was to examine whether, and to what extent, fear of contracting Covid-19 and compliance with the mandatory rules of isolation affected Polish adults’ nutritional behaviors. The online study was carried out during the first wave of the pandemic on a sample of 926 adults. Through cluster analysis, three groups of respondents were isolated: 1. People who fear a Covid-19 infection and follow the isolation rules (FFR), 2. People moderately afraid of the disease and following the rules loosely (MFFR), 3. People who are not afraid of the infection and do not follow the rules of isolation. (NFFR). The clusters were profiled with consideration of different aspects of eating behaviors as well as socio-demographic and economic features. The results of the study show a close relationship between the level of fear of contracting Covid-19 and the degree to which isolation rules are followed. These two factors were found to have a significant impact on eating behaviors, such as food purchases, eating patterns, and levels of consumption. It was stated that the FFR group changed their eating behaviors the most in terms of food purchasing, eating habits (excluding diversity and quality of diet), and food product consumption. The greatest stability in the majority of the analyzed areas of nutritional behaviors was observed in the MFFR cluster. The NFFR group shown the greatest decrease both in regularity and quality of their meals. This group also exhibited a significant increase in the consumption of alcoholic beverages. The results of the study can be useful in the decision making process when introducing restrictions or managing information. They also point to the need for extensive nutritional education focused on explaining the relationship between nutrition and health during a pandemic.

Synthesis and Characterization of Y2O3:Eu3+ Film Derived by Sol-Gel Processing

2007 ◽  
Vol 336-338 ◽  
pp. 593-596
Author(s):  
Chun Yang ◽  
Jun Ying Zhang ◽  
Hai Bing Feng ◽  
Wei Chang Hao ◽  
Tian Min Wang
Keyword(s):  
Raw Materials ◽  
Sol Gel ◽  
Emission Spectra ◽  
Luminescent Properties ◽  
Dip Coating ◽  
Organic Additive ◽  
Excitation Spectra ◽  
Excitation Peak ◽  
Close Relationship ◽  
Dip Coating Technique

Y2O3:Eu3+ thin film was synthesized by sol-gel method with inorganic salt raw materials, and the crystal structure and luminescent properties were investigated. By adding organic additive to the sol, a homogeneous film with high luminescent intensity could be obtained by dip-coating technique on the surface of alumina sheet and quartz glass. Structures of the films were studied by XRD and SEM. The excitation spectra of the films showed a wide excitation peak from 200nm to 260nm, and the emission spectra had a strongest emission peak at 611nm which revealed a close relationship with the calcining temperature.

scholarly journals CRISPR-Cas9: A Powerful Tool to Efficiently Engineer Saccharomyces cerevisiae

Life ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 13
Author(s):  
João Rainha ◽  
Joana L. Rodrigues ◽  
Lígia R. Rodrigues
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Genome ◽  
Specific Method ◽  
Complex Processes ◽  
Biological Studies ◽  
Precise Tool ◽  
Long Time ◽  
Gene Knockouts ◽  
Heterologous Pathway ◽  
A Current

Saccharomyces cerevisiae has been for a long time a common model for fundamental biological studies and a popular biotechnological engineering platform to produce chemicals, fuels, and pharmaceuticals due to its peculiar characteristics. Both lines of research require an effective editing of the native genetic elements or the inclusion of heterologous pathways into the yeast genome. Although S. cerevisiae is a well-known host with several molecular biology tools available, a more precise tool is still needed. The clustered, regularly interspaced, short palindromic repeats–associated Cas9 (CRISPR-Cas9) system is a current, widespread genome editing tool. The implementation of a reprogrammable, precise, and specific method, such as CRISPR-Cas9, to edit the S. cerevisiae genome has revolutionized laboratory practices. Herein, we describe and discuss some applications of the CRISPR-Cas9 system in S. cerevisiae from simple gene knockouts to more complex processes such as artificial heterologous pathway integration, transcriptional regulation, or tolerance engineering.

scholarly journals Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production

2017 ◽  
Vol 39 (2) ◽  
pp. 189
Author(s):  
Flávia Deffert ◽  
Bruna Carla Agustini ◽  
Geraldo Picheth ◽  
Tania Maria Bordin Bonfim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Maximum Concentration ◽  
Lower Cost ◽  
Yeast Species ◽  
Molecular Techniques ◽  
Free Cells ◽  
Influence Of Ph

Fructooligosaccharides are catalyzed by β–fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L-1 sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v-1), and Saccharomyces cerevisiae 222 produced 1-kestose and nystose. 

scholarly journals TECHNICAL AND TECHNOLOGICAL SOLUTIONS FOR PREPARING FLAX RAW MATERIALS FOR PROCESSING

2021 ◽  
pp. 227-237
Author(s):  
Yu. Вerezovsky ◽  
T. Kuzmina ◽  
M. Yedynovych ◽  
G. Boyko ◽  
N. Lyalina ◽  
...  
Keyword(s):  
Raw Materials ◽  
Hollow Structure ◽  
Weather Conditions ◽  
Raw Material ◽  
Hollow Structures ◽  
Adverse Weather Conditions ◽  
Adverse Weather ◽  
Long Time ◽  
Experimental Researches

The article contains theoretical and experimental researches in the field of the preservation of flax raw material of high moisture content. In the article, factors that are worsening the quality of flax raw material, resulting in non-observance of agrotechnical and technological requirements of preparing, collecting, harvesting the stem material, adverse weather conditions and other factors, are considered. The objective of this paper is to study the influence of preservation agents’ concentrations and of hollow structure device on the quality of flax raw material during long-time storage. In the article, the influence of aqueous preservatives’ concentration, humidity, storage length on the quality of fibrous products, obtained as a result of processing stem material, is analyzed. The influence of the device of hollow structures, as an alternative to preservatives, on the storage process of bast crops stem material was evaluated. The method of flax retted straw storage is described, actions of the main factors influence on the strength of fibers are analyzed. It is demonstrated, that prolongation of flax raw material preservation time can be made by using preservatives without considerable wastes of quality.

scholarly journals Plans d’eau glaciaires et isostasie dans les bassins de la haute Ware et de la rivière Otter, Massachusetts (2e partie)

2005 ◽  
Vol 17 (41) ◽  
pp. 251-264
Author(s):  
Gilbert Cestre
Keyword(s):  
New England ◽  
Eastern Canada ◽  
Low Area ◽  
Proglacial Lakes ◽  
Close Relationship ◽  
Long Time ◽  
History Of ◽  
Connecticut Valley ◽  
The City ◽  
Detailed Work

The present study is one of the unpublished research projects which are known to have been conducted in New England and in Eastern Canada under the guidance of the late Richard J. LOUGEE, long-time professor of Geomorphology at Clark University. Over a number of years, this writer has worked in close relationship with Lougee and much evidence in the field was studied together. It is believed that here has been recorded a most detailed work of surveying, and this undoubtedly accounts for the somewhat exceptional results that will be presented. The area selected for this study (about 80% of it is woodland) is located in the highlands of Central Massachusetts in Worcester County, about twenty miles (32 kilo-meters) northwest of the city of Worcester. It consists of the valley of the Otter River draining north, and of a small portion of the East Branch of the Ware River draining south. Since completion of this study, parts of the low area which held the ancient glacial lakes have been flooded to become water reservoirs. That proglacial lakes, though temporary they may have been, once submerged much of the area under study, is shown by an abundance of deltas, kames, eskers and deltaic kames terraces. It is believed that all of these were built under water in such lakes. Other features, such as kettle-holes and glacial outlets, especially ice-marginal channels cut diagonally down the slope, have also been studied. By plotting on a profile of the most characteristic elevations (often carefully surveyed), it is possible to find the water planes of ancient proglacial lakes. To this must be added experiments conducted in a sedimentation tank as also measurements of both the imbrication of cobbles in eskers and the « smoothness indexes » of such stones and pebbles, using A. Cailleux' methods. Thus were obtained results which tend to show that : 1- the area under study probably was in a deep interlobate space created between the Connecticut Valley lobe to the west and the Boston Basin lobe to the east ; 2— ice-marginal channels are an indication of the existence of a thick, fast-retreating ice border ; 3- an isostatic balance restored itself by sometimes quick and strong adjustments of the crust of the earth ; 4— an early upwarping, made up of various zones of tilting articulated on hinge lines, has been referred to as Hubbard Uplift and is the earliest known in the post-Glacial history of New England.

scholarly journals Influence of organic acids and organochlorinated insecticides on metabolism of Saccharomyces cerevisiae

2005 ◽  
pp. 207-215 ◽  
Author(s):  
Dusanka Pejin ◽  
Vesna Vasic
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Sugar Beet ◽  
Specific Growth Rate ◽  
Glycogen Content ◽  
Specific Growth ◽  
Raw Materials ◽  
Stress Factors ◽  
Adaptive Mechanism ◽  
Ribonucleic Acids

Saccharomyces cerevisiae is exposed to different stress factors during the production: osmotic, temperature, oxidative. The response to these stresses is the adaptive mechanism of cells. The raw materials Saccharomyces cerevisiae is produced from, contain metabolism products of present microorganisms and protective agents used during the growth of sugar beet for example the influence of acetic and butyric acid and organochlorinated insecticides, lindan and heptachlor, on the metabolism of Saccharomyces cerevisiae was investigated and presented in this work. The mentioned compounds affect negatively the specific growth rate, yield, content of proteins, phosphorus, total ribonucleic acids. These compounds influence the increase of trechalose and glycogen content in the Saccharomyces cerevisiae cells.

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